Often in laboratory experiments you will want to amplify regions of the genome from several different preparations of gDNA. To do this, a mastermix will be created with all the common reagents to ensure easier pipetting, proper mixing, and controlling for variations in pipetting. Create a mastermix for running 8 identical reactions, including one negative control, each with different gDNA templates (7 total gDNA templates).
Answer:
Mastermix in PCR helps in providing faster and easier setup with pipetting, proper mixing, reduction in contamination and controlling fir variation in pipeting or tube to tube variability. Contents of PCR master mix for the desired reaction are as follows:
dNTPsPrimersthermostable DNA polymerasecofactor (MgCl2)Template DNA (for 7 samples)PCR-grade waterThe only difference in negative control for the PCR master mix is template or the different primer rest the components are the same in the master mix.
The Rh factor is also known as the
antigen.
A. monoclonal
B. polyclonal
C. D
D. X
E. AB
Answer:
Explanation:
Option E is the correct answer
Answer:
C. D
Explanation:
RBCs that are Rh positive express the one designated D (RhD antigen).
-TheUnknownScientist
HELP!!!! Two characteristics of most plants are a root system and a shoot system. Most of the structures in these systems
are common to all plants; however, there are some variations. Which structure can sometimes be found as part of
the shoot system?
stems
leaves
roots
flowers
Answer:
Leaves, and flowers
Explanation:
The shoot system is above ground and includes the organs such as leaves, buds, stems, flowers (if the plant has any), and fruits (if the plant has any).
Answer:
ROOTS
Explanation:
ROOT ROOT ROOT ROOT ROOT ROOT because it root
The graphs show the reaction rate for an enzyme across a range of temperatures and pH. Based on these data, this enzyme functions best at what temperature and pH? *
The graph is attacged and the options is as follows:
Temperature of 27oC and a pH of 4
Temperature of 37oC and a pH of 6
Temperature of 40oC and a pH of 8
Temperature of 50oC and a pH of 10
Answer:
The correct answer would be - temperature of 37oC and a pH of 6
Explanation:
The graph 1 shows the relation between the rate of reaction for an enzyme and the temperature of reaction is been represented. In this graph the rate of eaction is highest (point from the slope starts decreasing) at the So, by the options and graph the temperature 37°C(Below 40°C) rate of reaction is maximum.
Similarly, in other graph rate of reaction and and pH has been represented in which rate of reaction is maximum at pH 6. after 6 pH the rate of reaction started decreasing.
Cells use different processes at different times to provide an organism with the energy that it needs. Spirulina is an autotrophic bacteria that can be found in freshwater and marine environments. Which process takes place in the cells of Spirulina that does not occur in the cells of a human? photosynthesis aerobic respiration anaerobic respiration cellular respiration
photosynthesis
aerobic respiration
anaerobic respiration
cellular respiration
Answer:
I believe it is d, cellular respiration.
Explanation:
Answer:
D
Explanation:
Wave energy causes sound energy and light energy
True or False
please help me :(((((
Please help!!!!!!!!!!!!!
Some organisms, such as bacteria, reproduce asexually; whereas, other organisms reproduce
sexually. Which statement correctly states why one type of reproduction is beneficial over the
other?
A. Asexually reproduced offspring are more genetically diverse.
B. Asexual reproduction produces offspring genetically different from parents.
C. Sexual reproduction can produce a large number of offspring very quickly.
D. Sexual reproduction produces organisms that are genetically diverse.
Answer:D
Explanation: A isn't right because during asexual reproduction there is no exchange of genetic characteristics thus the offsprings retain the genetic characteristics of their parents and there is no diversity.
Sexual reproduction on the other hand involves the fusion of gametes and the exchange of genetic characteristics thus the offsprings are diverse and are not identical to their parents as is the contrary in asexual reproduction.
Sexual reproduction produces organisms that are genetically diverse.
Sexual reproduction involves the combination of chromosomes from both parents. This combination of chromosomes introduces variation into the population. This combination does not occur in asexual reproduction.
Hence, the statement that correctly states why one type of reproduction is beneficial over the other is that sexual reproduction produces organisms that are genetically diverse.
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Brainliest Which of the following best explains how most plants grow? *
Plants store the excess oxygen they produce, resulting in them gaining mass
Plants store the excess glucose they produce, resulting in them gaining mass
Plants store the excess carbon dioxide they produce, resulting in them gaining mass
Plants store the excess water they produce, resulting in them gaining mass
Answer:
plants store the glucose they produce, resulting in them gaining mass. that should be the correct answer
what must happen before miosis can begin?
Answer:
Before meiosis actually begins, the DNA that is packaged into chromosomes must be fully copied. ... DNA replication occurs in the same fashion as it does during mitosis. After replication, the homologues are doubled, and each chromosome now has a homologous pair
The width of a rectangle is 3 inches less than the length. The perimeter is 38 inches. Find the length and the
width.
The length is___
inches and the ____ width is
inches.
Answer:
25
10
Explanation:
Hope this helps:)
Which describes a eukaryotic cell, but not a prokaryotic cell?
A: is surrounded by a cell membrane
B: makes and uses proteins
C: contains DNA in a nucleus
Answer:
c?
Explanation:
eukaryotes have a nucleus and prokaryotes do not
Answer:
C.) Contains DNA in a nucleus
Explanation:
There are several differences between the two, but the biggest distinction between them is that eukaryotic cells have a distinct nucleus containing the cell's genetic material.
Energy is defined as the ability to
A. reproduce.
B. grow.
C. do work.
Answer:
its c
Explanation:
Answer:
C. to do work
Explanation:
true or false , the mRNA codon UGG is the only codon that codes for the amino acid tryptophan
Answer:
true
Explanation:
how do i turn off the internet while choking bart simpson
Answer:
good question, you don't. Or simply wait untill you finish choking bart
Which type of speciation is based on a small number of individual being
introduced into a new environment?
Answer:
peripatric speciation
Explanation:
When small groups of individuals break off from the larger group and form a new species, this is called peripatric speciation
1 point
A man with type A blood marries a woman with type AB blood. Based on
what you know about blood types, what is the only blood type not possible
in any of their children? (remember, the 4 types are A, B, AB, and O)*
A
B
AB
0
They can't have a child with blood type O(I) because one of the parents has blood group AB(IV)
!!!!PLEASE HelP!!!!
A farmer has decided to dig an artesian well into the unconfined aquifer below his crops. However, water does not fill his well. Why isn't his well working?
A. An artesian well needs the pressure of a confined aquifer
B. An artesian well must be near a lake
C. An artesian well must be near the site of groundwater infiltration
D. An artesian well only works in areas that are going through a drought
Answer:
it is a
Explanation:
i did the test
A farmer has decided to dig an artesian well into the unconfined aquifer below his crops. His well is not working because an artesian well needs the pressure of a confined aquifer. The correct option is A.
What is an aquifer?An artesian well is a deep tube well drilled underground to gather water for human usage. This good type is found in restricted aquifers.
The artesian well differs from the others in that it does not require the use of a pump to raise the water, as the cistern does. The sort of subsurface reservoir causes the water that travels through the tube to naturally spurt to the surface.
The underground reservoir with the qualities required for drilling an artesian well is the product of extremely specialized geological conditions. Gushing wells are caused by the restricted aquifer, as this sort of deep groundwater is known. This type of reservoir is found between impermeable subsurface layers.
Therefore, the correct option is A. An artesian well needs the pressure of a confined aquifer.
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Please help me on this history please I’m begging also it’s number one not number 2
Answer:
3 Or 2 I think?
Explanation:
So Sorry If It isn't right!
would an electric eel be able to shock predators if its cells run low on ATP?
Answer: no.
Explanation:
A scientist is developing a potential treatment for cancer. She grows cells in hundreds of petri dishes and exposes the dishes to environments that are known to cause cancer. After exposure, the scientist determines that some of the petri dishes have cancer cells.What factor do all the petri dishes that contain cancer cells have in common?
Answer:
they are normal mutations you can never have a cure to cancer you would have to throw a lot of your DNA and genes away to have a less chance of dying but we could slow or kill cancer but no way to prevent it from happning as it happens everyday to you but those cells have a higher chance to become cancer becuase of those thing that help cells to become cancer.
Explanation:
Horses with genotype (BB) have a brown coat, horses with genotype (bb) have a white coat,
and horses with genotype (Bb) have a tan colat. Based on the information provided, the
alleles controlling coat color in horses show:
Answer: Incomplete dominance
Which organelle is used for transportation?
A.nucleus
B. Golgi bodies
C. Cell membrane
D. Endoplasmic reticulum
Can you combine an apple and an orange to make a cross hybrid fruit? (Not sure this classify as biology)
Answer:
No
Explanation: Apples and Oranges are different species, therefore they are unable to interbreed. This would only be possible by gene splicing (Gene splicing is a form of genetic engineering where specific genes or gene sequences are inserted into the genome of a different organism) which is incredibly difficult.
what is meant by the term VO2 max
Answer:
VO2 max is the maximum volume of oxygen you can consume per minute per kilogram of body weight at your maximum performance. VO2 max is an indication of aerobic fitness and should increase as your level of fitness improves.
Answer:
VO2 is the maximum oxygen uptake.
Explanation:
VO2 is the maximum oxygen consumption during incremental exercise.
Ann is looking at the night sky. There is a first-quarter moon. What does she
see? *
a moon shaped like a crescent
a moon shaped like a half circle
a moon shaped like a circle, shining brightly
no moon in the sky
Answer:
A Moon shaped like a half circle
Explanation:
Answer:
a moon shaped like a half circle
Explanation:
WILL MARK BRAINLIEST! :)
Diversity occurs in many places in meiosis. In what process is the first place where the pieces of DNA switch positions with the homologous sister chromatids?
A. Interphase
B.Independent Assortment
C. Crossing Over
D.Anaphase
E. Randomness of egg and sperm meeting
Answer:
crossing over
Explanation:
I had it on a bio exam. i got it right
In what part of the body would you find the fibula?
Answer:
you can find it in the leg!! The fibula is a bone located within the lateral aspect of the leg!!
Explanation:
Answer:
The fibula is a bone located within the lateral aspect of the leg.
Explanation:
Zacharias Jansen was known for being a spectacle maker and is credited with making the 1st
microscope.
True or False?
Answer:
True
Explanation:
Zacharias Jansen was known for being a spectacle maker and is credited with making the 1st microscope!
Convection is the movement of material that distributes heat in the Earth's mantle. What arrangement of material may lead to convective motion?
A. Denser material on top of less dense material
B. Less dense material on top of denser material
C. Mixed material with a uniform density
D. Mixed material with a uniform temperature
Answer:
it is less dense on top of denser material. B
Answer:
A. Denser material on top of less dense material
Explanation:
Took a quiz and got it right.
Which of the following base pairs would be found in a cell
The base pair cytosine and guanine is found in the cell. The correct option is D.
What are base pairs?A base pair is a primary component of double-stranded nucleic acids that comprises two nucleobases that are hydrogen-bonded to one another.
They are the foundations of the DNA double helix and make a contribution to both DNA and RNA's folded structure.
Purines and pyrimidines are nitrogen bases that form hydrogen bonds between DNA strands.
Predicated by Chargaff's Rule, they associate together via complementary pairing A::T and G::C. The purines in DNA are the same as those in RNA: adenine and guanine.
The 2 significant pyrimidine bases in DNA are cytosine and thymine, which base pair with guanine and adenine. Uracil supplants thymine and base pairs to adenine in RNA.
Thus, the correct option is D.
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Your question seems incomplete, the probable missing options are:
A) adenine-guanineB) cytosine-thymineC) uracil-cytosineD) cytosine-guanine