You decide to focus first on Mutation 1 and Mutation 2.
The F' plasmid appears to "rescue" mutant strain 1 by restoring wild-type inducible expression.
The F' plasmid does not change the phenotype of mutant strain 2; there is still no expression of the lac operon.
There are three possible mutations that could lead to lack of expression: a super-repressor (Is), a promoter mutation (P-) or a mutation in the lacZ gene (Z- ). To determine the effect of introducing a wild-type F' plasmid into a bacterial cell with each of these mutations, you draw out the three possible scenarios.
F' l+ cap+
Determine the expression of the lac operon for each possible partial diploid and for the "unknown" mutation partial diploids (Mutation 1 and Mutation 2).
1. F' lac+/ ts
2. F' lac+/ P
3. F' lac+/ Z
4. F' lac+/ Mutation 1
5. F lac+/ Mutation 2
A. Inducible (wild type)
B. No expression (mutant)
Answer:
Super-repressor (Is) - Uninducible dominant
Binding region is modified by the protein called repressor which prevents the lactose to bind with it. Thus, even the lactose is present the repressor protein is bound to the promoter and can not be detach from it. Transcription is not inducible.
Promoter mutation (P)- - Uninducible recessive
Mutated promoter region inhibits the binding of RNA polymerase that leads to trranscription.
The lacZ gene ( Z-) is mutated - recessive while the normal LacZ gene (Z +) - dominant .
Inducible (Wild type) No expression (Mutant)
F' lac+/ Is No Yes
F' lac + / P- Yes No
F' lac + / Z- Yes No
F' lac +/ Mutation1 Yes No
F' lac+ / Mutation2 No Yes
Mutation 1 and F' plasmid - Inducible wild type
Mutation 2 and F' plasmid - Mutant no expression
In which of the following ways is DNA replication different from DNA transcription?
Answer:
Thymine is used in replication while uracil is used in transcription.
Explanation:
New cells can only be created after transcription has taken place. Replication results in mRNA strands while transcription creates new DNA. Transcription happens outside the nucleus and replication happens inside the nucleus. Thymine is used in replication while uracil is used in transcription.
DNA replication is a process whereby DNA makes a copy of itself while DNA transcription is a process whereby a messenger RNA is synthesized from a DNA strand.
Both processes involve the building-up of nucleotides or purine.pyrimidine bases. Replication involves building up of cytosine, guanines, adenine, and thymine bases while in transcription, the thymine base is replaced by uracil base.
Hence, the correct answer is that Thymine is used in replication while uracil is used in transcription.
y= -14x + 1.2 5 can be x or y
Answer:
Slope= 2.0002.400=1.200
x−intercept=−60=−0.00000
y−intercept=50=0.00000
Hoped I helped
S100A8 sex chromosome? yes or no
Answer:
yes !!
Explanation:
because it's yes !!
Answer:
YES
Explanation:
I know this because i looked it up
g Riboswitch is a mechanism of gene activity regulation by mRNA conformation. Riboswitch can regulate ______.
Answer: c. both transcription and translation
Explanation:
Riboswitchs are mRNA elements that influence the activity of genes certain genes by regulating same and are found in other organisms apart from humans such as bacteria, plants and fungi.
They regulate activity by binding to specific small molecules known as ligands thus causing a structural rearrangement that enables either transcription or translation to be modulated in the gene.
Which statement explains how a DNA mutation causes sickle-cell anemia? (point)
The DNA mutation increases the number of amino acids in hemoglobin, which causes a change in the shape of red blood cells.
The DNA mutation causes a change in the amino acid sequence for hemoglobin, which causes a change in the shape of red blood cells.
The DNA mutation decreases the number of amino acids in hemoglobin, which causes a change in the shape of red blood cells.
O The DNA mutation prevents formation of hemoglobin, the absence of wirich causes a change in the shape of red blood cells.
Answer:
The DNA mutation causes a change in the amino acid sequence for hemoglobin, which causes a change in the shape of red blood cells.
Explanation:
Sickle cell anemia is one of a group of disorders known as sickle cell disease. Sickle cell anemia is an inherited red blood cell disorder in which there aren't enough healthy red blood cells to carry oxygen throughout your body.
Normally, the flexible, round red blood cells move easily through blood vessels. In sickle cell anemia, the red blood are shaped like sickles or crescent moons. These rigid, sticky cells can get stuck in small blood vessels, which can slow or block blood flow and oxygen to parts of the body.
Sickle cell anemia is caused by a mutation in the gene that tells your body to make the iron-rich compound that makes blood red and enables red blood cells to carry oxygen from your lungs throughout your body (hemoglobin). In sickle cell anemia, the abnormal hemoglobin causes red blood cells to become rigid, sticky and misshapen.
The sickle cell mutation reflects a single change in the amino acid building blocks of the oxygen-transport protein, hemoglobin. This protein, which is the component that gives red cells their color, has two subunits. The alpha subunit is normal in people with sickle cell disease. The beta subunit has the amino acid valine at position 6 instead of the glutamic acid that is normally present. The alteration is the basis of all the problems that occur in people with sickle cell disease.
The DNA mutation causes a change in the amino acid sequence for hemoglobin, which causes a change in the shape of red blood cells.
What is mutation?A mutation is an alteration in the nucleic acid sequence of the genome of an organism, virus, or extrachromosomal DNA. Viral genomes contain either DNA or RNA.
Moreover, a mutation is a change in the DNA sequence of an organism. Mutations can result from errors in DNA replication during cell division, exposure to mutagens or a viral infection.
Therefore, when a gene mutation occurs, the nucleotides are in the wrong order which means the coded instructions are wrong and faulty proteins are made or control switches are changed. The body can't function as it should. Mutations can be inherited from one or both parents.
Learn more about mutations:
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How can particle interactions explain the properties and behavior of dry ice?
Answer:
Dry ice is the solid form of carbon dioxide (CO2). The particle interaction in dry ice is due to collision between inter-particles which results in agglomeration and shows the properties and behavior of dry ice.
The inter-particle collision in dry ice keeps the high temperature of dry-ice and make it sublimate (solid to gas) at room temperature and normal pressure.
The collision of carbon atoms in dry ice also makes it highly reactive and shows chemical properties of dry ice. When dry ice is added with magnesium, it left black powder, which is carbon
In prokaryotic cells, regulator proteins bind to a section of DNA called alan (1 point)
O repressor
o promoter
O chromatin.
o operon.
Answer:
promoter
Explanation:
Answer: Operon
Explanation:
When a liquid becomes a gas, _____
The molecules move closer together
The molecules move farther apart
The molecules are unchanged
There are no molecules
Answer:
The molecules move farther apart.
Saprobic microorganisms are important decomposers of plant litter, animal matter, and dead microbes. This is an example of a(n) ______________.
Answer:
The correct answer would be - heterotrophs (chemoheterotrophs)
Explanation:
Heterotrophs are the living organisms that are directly or indirectly depend on the autotrophs or the organisms that can make their own food for energy and growth.
Saprobic microorganism are free living organisms that get their energy from decomposing rotten or dead organic matter such as plant litter, animal matter and dead microbes. It is performed with the help of enzymes or chemicals they release to decompose such matter. they also known as chemoheterotrophs.
Thus, the correct answer would be - heterotrophs (chemoheterotrophs).
If a mother is affected by an X-linked dominant condition and the father is not, which children can inherit the condition?
only males
neither males nor females
both males and females
only females
Answer:
both males and females
Explanation:
The sex chromosomes in male and female humans are X and Y chromosomes. X-linked traits are those traits which are associated with the X- chromosome.
According to the question, the trait is passed on a X-linked dominant condition, which means that the possession or not of the condition is dependent on the presence of the dominant X-chromosome. The dominant X-chromosome will always express itself even when in an heterozygous state with a normal X-chromosome i.e Xx. Hence, only a individual recessive for the X-chromosome will be normal.
A mother that is affected by the X-dominant condition will either possess a; XX or Xx genotype while a father that is normal (not affected) will possess a (xY) genotype. Since the mother passes one of her X chromosomes to her sons and the other to her daughters, both male and female children will be affected by the condition.
N.B: If the mother is heterozygous for the affected X-chromosome (Xx), half of her sons and half of her daughters will inherit the condition but if the mother contains two dominant X-chromosomes (XX), all of her sons and daughters will inherit the condition.
Mandy and Pat are discussing a movie that showed someone being injured by radio waves. Mandy says that's not likely because
radio waves are low frequency and low energy, and Pat says that it is likely because otherwise they would not put it in a movie.
What is the best way to help them decide if the information is true?
They can write a letter to the director.
Movies are reliable sources, so they can assume Pat is correct.
They can use reliable web sites or books to get more information.
They can watch another movie in the series to see what happens next.
Answer:
Its C, They can use reliable web sites or books.
Explanation:
In pea plants there is a dominant allele (A) for green pods and a recessive allele (a) for yellow pods. Suppose a heterozygous plant is crossed with a plant that has yellow pods. Complete the sentences about this cross with the correct terms.
1. The phenotype of the heterozygous plant is_________.
2. The genotype of the heterozygous plant is_________.
3. The genotype of the plant with yellow pods is________.
4. The genotypes of gametes produced by a heterozigous plant is______.
5. The genotypes of gametes produced by a yellow plant is_______.
A. Green pods
B. 75% AA and 25% Aa
C. AA
D. 100% A
E. Yellow pods
F. 50%
G. 25%
H. 100% a
I. 50% A and 50% a
J. Aa
K. 0%
L. 75%
M. 75% A and 25% a
Answer:
1. Green
2. Aa
3. aa
4. A and a
5. a and a
Explanation:
1. The phenotype of the heterozygous plant (Aa) would be green since the allele responsible for green color (A) is dominant over the allele responsible for yellow color (a).
2. The genotype of a heterozygous plant would be Aa. Heterozygous individuals have different alleles for the same gene.
3. The genotype of the plant with a yellow pod would be aa. Since the allele for green color (A) is dominant over the allele for yellow color (a), the yellow color can be expressed only in the absence of the (A) allele. Hence, the genotype fo yellow color has to be aa.
4. The genotypes of gametes produced by a heterozygous plant would be (A) and (a) because heterozygous individuals have two different alleles for a gene.
5. The genotypes of gametes produced by a yellow plant would be (a) and (a) because a yellow plant has the genotype (aa).
Given the gene sequence GGACCGTCGATCTTC, which of the following choices would
represent an inversion mutation?
A. CCACCGTCGATCTTC
B. GGACCG TCGATC TTC
C. GGACCGTCGATCCTT
D. GGAC TCGATC TTC
Answer:
I would go with A maybe,am not really sure though.
DNA and RNA are structurally similar in some ways, but different in others. Identify whether each of the following statements applies to DNA, RNA. both or neither.
1. It can contain the purine adenine.
2. It can contain the pyrimidine uracil.
3. This contains the pyrimidine thymine.
4. In terms of base composition, the %T = %G.
5. This contains the sugar ribose.
6. The sugars are connected with a 3'-5' phosphodiester link.
7. It contains equal amounts of guanine and cytosine.
8. Bases are attached to sugars in an alpha N-glycosides linkage.
Answer:
DNA
It can contain the purine adenine
This contains the pyrimidine thymine
The sugars are connected with a 3'-5' phosphodiester link.
It contains equal amounts of guanine and cytosine. The percentage of A-T ,C-G are the same in DNA,,they are linked by hydrogen bonds.But varies in RNA.
Explanation:
.RNA
It can contain the purine adenine.
It can contain the pyrimidine uracil
This contains the sugar ribose. This is a 5C sugar with one oxygen atom deficient.The forms the lever for holding the bases and phosphate group
The sugars are connected with a 3'-5' phosphodiester link.This is the backbone of both DNA and RNA.It is formed between the sugars and the phosphate groups in both DNA and RNA.
None is made of base composition, the %T = %G. This is wrong because purines must pair with pyrimidine,but not two purines or pyrimidine pairing as shown,
Bases are attached to sugars in an alpha N-glycosides linkage.This is wrong the beta N-glycisidic bonds are the linkages
o help create these gradients, there are a series of capillaries, branching from the peritubular capillaries and associated with the loop of Henle, collectively called the
Answer:
Vasa recta.
Explanation:
Vasa recta are a group of capillaries, branching from the peritubular capillaries and moves parallel with the loop of Henle. Vasa recta is responsible for the supply of blood to renal medulla in order to purify the blood from the waste materials such as salts, excess water and nitrogenous waste etc. Due to its movement with the loop of Henle, it slows down the blood flow which helps in the re-absorption of water.
Two of the many cycles in nature's web of life are the carbon cycle and the A. energy cycle B. water cycle C. food cycle D. heat cycle
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▹ Answer
B. water cycle
▹ Step-by-Step Explanation
Water is something that is dependent on amongst living things. Without the water cycle, there wouldn't be a source of water and living things wouldn't be able to survive.
Hope this helps!
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4. Unlike a scientific theory, a scientific law describes an observed pattern in nature without
attempting to explain it.
O true
Ofalse
Answer:
False
Any discovery must first be tested and have an explanation
how gravity plays a role in the parts of the water cycle shown in this model.
Answer:
look dear i will explain
Explanation: It pulls precipitation down from clouds and pulls water down hill . gravity also moves air and ocean water. Gravity pulls denser air and water downwards,forcing less dense air and water o move upwards.
okay hope it works
Hit like
Answer:
Gravity causes precipitation in the clouds to fall to Earth. On the ground, gravity influences surface runoff, as water travels from higher to lower elevations. Gravity also influences groundwater because it pulls elements toward the center of Earth. Water, under the influence of gravity, seeps into the ground to form groundwater.
Explanation:
there u go guys thats the sample answer but change it up please. have a good day
Which is the best way to describes the Biodiversity of an areas
Answer:
Biodiversity is defined as “the variability among living organisms from all sources including, inter alia, terrestrial, marine and other aquatic ecosystems and the ecological complexes of which they are part; this includes diversity within species, between species and of ecosystems.” The importance of this definition ...
on terms of genetic similarity, which list is ordered from most similar to least similar b. cousin, uncle, mother, non twin sibling, identical twin
Answer:
Identical Twin, non twin sibling, Mother, Cousin, Then Uncle
Explanation:
According to kirk smith a professor of environmental health at the university of california berkley indoor fires increase risks of pneumonia tuberculosis lung cancer and low birth weight in babies born of women expose during pregnancy. What simple solution is being widely promoted to reduce the risk of death?
a) preparing meals using solar cookers.
b) switching from wood to burning crop waste as a fuel source.
c) adding more windows to houses as a source of ventilation.
d) passing a green tax to make homeowners pay for their pollution.
e) providing asthma inhalers to children under the age of 12 years.
Answer:
preparing meals using solar cookers.
Explanation:
solar cookers radiates at low rates.Therefore the most of the nutrients in the foods are conserved.Thus most micro nutrients for biochemical activities are retained. Vitamins which can not withstand high temperature are preserved.
Most importantly carcinogens which are usually associated with high heat foods are avoided ,when cook with low heat of solar cookers
The solar cooker is smoke free,therefore irritation of the lungs,lung cancer associated with high heat cookers is avoided.
Specifically,local Mayan women exposed to high heat smoke cookers, suffers lung cancer,and those with pregnancy gives to infants with low birth weights. Children exposed to theses high heat also experienced acute lower respiratory infections.
Thus the smokeless,low heat solar cooker is safer.
An area classified as a temperate deciduous forest begins to experience a lack of rainfall that lasts for many years. Given enough time, if the area continues to experience a lack of rainfall, it will eventually become:__________a. savanna.b. temperate rainforest.c. shrubland, grassland, or woodland.d. taiga.
Answer:
Savanna
Explanation:
Lack of rainfall over a period of time may convert a temperate deciduous forest into a Savanna.
Grasslands are characterized by a domination of grasses rather than large shrubs or trees. Savanna is a grassland with a few scattered individual trees. Savannas are always found in areas that are quite hot. The annual rainfall in a typical savanna may range from about 76.2-101.6 cm. Some savanna regions may receive as low as 15.24 cm -25.4 cm of rain in a year, followed by a long period of drought when fires can occur.
What term is used to describe a retrograde flow of urine from the urinary bladder into the ureters that is the cause of recurrent urinary tract infections (UTIs)?
Answer:
Vesicoureteral reflux (VUR)
Explanation:
Answer:
The condition is known as Vesicoureteral reflux (VUR)
Explanation:
Vesicoureteral reflux (VUR) is a pathological condition caused by Urinary Tract Infections (UTIs) where urine flows back from the bladder into one or both ureters, and sometimes back into the kidneys. The condition is commonly seen in young children under 2 years of age, and infants.
Most children with primary VUR were born with an abnormal ureter, which is mainly seen as a defective valve between the kidneys and the ureters that does not close well, hence resulting in refluxes. Secondary VUR develops due to some pathological conditions affecting the urinary tract, like blockage of the urethra, causing reflux of urine. Defective bladder nerves can also lead to VUR.
The ion composition of perilymph is like that of interstitial fluid; high sodium and low potassium levels. If someone ruptured the vestibular membrane, the membrane between the vestibular duct (Scala vestibuli) and the cochlear duct (Scala media), what would be the effect of such a rupture on the concentration of potassium in the endolymph in the cochlear duct
Answer:
The concentration of potassium will decrease
Explanation:
The cochlear ducts are composed of two distict types of fluids: 1-perilymph, rich in sodium (140 mM) and low in potassium (5 mM), and 2- endolymph, rich in potassium (1 mM) and low in sodium (150 mM). The perilymph fluid has a similar composition to the rest of the extracellular fluids, while endolymph present a unique ion composition that is not found in other fluids of the body. It is believed that the cochlea can regulate potassium flow without using energy (i.e., without use ATP), since potassium enters into the cells through passive pathways, while this ion ion leaves the cells by an active mechanism.
Which of the following is NOT a factor of sustainability?
Group of answer choices
economics
ethics
biodiversity
natural capital
solar energy
Answer:
natural capital
HOPE IT HELPS!
PLS MARK AS BRAINLIEST!!!
You believe that the cytosolic protein p007 is involved in mediating growth hormone-stimulated proliferation in cells. You wish to establish a cell model using your cultured fibroblasts. Provide clear explanations to the following, each worth 2.5 pts each. a) Describe how you would transfect the growth hormone receptor DNA into your cells to create stable cell lines, and include a description of the benefits of using stable cell lines versus transiently transfected cell line. b) Describe how you would measure specific receptor binding of GH to your stable cell line. c) Describe how you would measure growth hormone-induced proliferation in your stable cell line. Make sure to include controls. d) Describe how you would use RNAi to demonstrate the requirement for p007 in growth-hormone stimulated proliferation. Make sure to include controls.
Answer:
Following are the answer to this question:
Explanation:
In option a:
There are many ways, which can be defined as follows:
It keeping cells "qualified," use heat shock and handle ions like Ca and ice incubating.It uses Micro-injection with DNA. It uses the biolistics or even the process of gene-gun.The stable cell charts indicate simple and definitive outcomes when their relationship to specific results. Moreover, relative to temporary cell lines, findings are more reliable and error-free. These lines also easily break and much less.
In option b:
Radio tests including linking tests could be used
The Ligand Binding Assays is an analysis, that is based on the binding of ligands to antibodies, or other macromolecules. Its existence and distance of formed ligand-receptor complexes are defined using a system of measurement that is usually electrical or fluorescent.
In option c:
The method for measuring the levels of cell growth is accessible and the Another way is to measure the metabolism within the same cell. So many color pigments were also accessible that can permeate an organism as well as react with some proteins as well as other factors, and that can effectively identify a color final product.
Throughout relation to the amount of metabolism exercise, cell proliferation could be evaluated by researching one or even more cell markings. The BrdU limited liability assessment is a very well-published illustration.
Its specific antibody can detect BrdU and high BrdUincorporations levels are linked to high levels of cell proliferation. Command cell without DNA receptor growth hormone.
In option d:
The cell growth will stop after each use of RNA interference ( RNAi). The p007 formation as well as the effect on growth caused by the intrusion will be a goal. If development is delayed effectively, the theory will be tested successfully to a cell without any of the required DNA. The RNAi is the free cell
How do animals survive along the deeper parts of the seafloor? A They feed at the surface. B They feed on each other. C They photosynthesize.
Answer:
They photosynthesize..
Explanation:
There are several ways deep ocean animals survive in such an enviournment..Food is scare in much of the deep sea, in parts because photosynthesis only thakes place at the ocean's surface where there's sunlight. Most animals cope with this by begin very small and meeding less to eat of by growoing very slow..
Answer:
the answer is They photosynthesize..
Explanation:
If an experimenter wants to use the GFP method but needs to detect the presence or absence of several proteins at the same time, he can take advantage of mutational variants of GFP that emit what light?
Answer:
different colors
Explanation:
The green fluorescent protein (GFP) is a type of protein widely used in molecular biology laboratories because this protein can be used to detect the expression of proteins and to identify cellular structures. This protein displays green fluorescence when it is excited by blue light and, in the last years, many variants of the GFP protein have been developed. The altered GFP proteins react to distinct wavelengths of light, thereby emitting light to different colors. The mutants forms of the GFP protein are produced by genome engineering techniques that generate modifications capable of altering the folding of the normal GFP protein.
Under normal conditions glomerular filtration depends on three main pressures. What pressures is a pressure that favors the process of filtration?
Answer:
Glomerular Hydrostatic pressure .
Explanation:
The basic function of the kidney is the formation of urine for elimination through the urinary excretory system. Two different processes determine this formation: the filtration of fluid through the glomerular capillaries into Bowman's space and the modification of the volume and composition of the glomerular filtrate in the renal tubules. The fluid passes from the glomerular capillaries to Bowman's capsule due to the existence of a pressure gradient between these two areas. This process is favored by two structural characteristics that make renal corpuscles particularly effective filtration membranes: glomerular capillaries have a much higher number of pores than other capillaries, and the efferent arteriole has a smaller diameter than the afferent arteriole, causing greater resistance to outflow of blood flow from the glomerulus and increasing glomerular hydrostatic pressure. Increased glomerular hydrostatic pressure (due to increased blood flow through the glomerulus) increases filtration, while increases in Bowman's hydrostatic pressure or urinary space (which remains constant, unless there is disease at that level, usually due to fibrosis) and plasma P. oncotic (determined by proteins, which tend to "drag" plasma into the glomerulus) decrease filtering. Resulting in a filtering pressure of 10 mmHg.
