Identify the first step in preparing a spectrophotometer for use.
A. Make sure all samples and the blank are ready for measurement.
B. Prepare a calibration curve.
C. Measure the absorbance of the blank.
D. Turn on the light source and the spectrophotometer.

Answers

Answer 1

Answer:

D. Turn on the light source and the spectrophotometer.

Explanation:

A spectrophotometer is a machine used to measure the presence of any light-absorbing particle in a solution as well as its concentration. To prepare a spectrophotometer for use, the first step is to turn on the spectrophotometer and allow it to warm up for at least 15 minutes. After this is done, the next step will be to ensure that the samples and blank are ready. Next, an appropriate wavelength is set for the solute being determined. Finally, the absorbance is measured of both the blank and samples.


Related Questions

A signalized intersection has a sum of critical flow ratios of 0.72 and a total cycle lost time of 12 seconds. Assuming a critical intersection v/c ration of 0.9, calculate the minimum necessary cycle length.

Answers

Answer:

[tex]T_o=82.1sec[/tex]

Explanation:

From the question we are told that:

Lost Time [tex]t=12secs[/tex]

Sum of critical flow ratios [tex]X=0.72[/tex]

Generally the Webster Method's equation for Optimum cycle time is is mathematically given by

 [tex]T_o=\frac{1.5t+5}{1-x}[/tex]

 [tex]T_o=\frac{1.5*12+5}{1-0.72}[/tex]

 [tex]T_o=82.1sec[/tex]

Imagine a cantilever beam fixed at one end with a mass = m and a length = L. If this beam is subject to an inertial force and a uniformly distributed load = w, what is the moment present at a length of L/4?

Answers

Answer:

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Explanation:

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The propeller shaft of the submarine experiences both torsional and axial loads. Draw Mohr's Circle for a stress element on the outside surface of the solid shaft. Determine the principal stresses, the maximum in-plane shear stress and average normal stress using Mohr's Circle.

Answers

Answer: Attached below is the missing detail and Mohr's circle.

i) б1 =  9.6 Ksi

б2 = -10.7 ksi

ii) 10.2 Ksi

iii)  -0.51Ksi

Explanation:

First step :

direct compressive stress on shaft

бd = P / π/4 * d^2

      = -20 / 0.785 * 5^2  = -1.09 Ksi

shear stress at the outer surface due to torsion

ζ = 16*T / πd^3

  = (16 * 250 ) / π * 5^3  = 010.19 Ksi

Calculate the Principal stress, maximum in-plane shear stress and average normal stress

Using Mohr's circle ( attached below )

i) principal stresses:

б1 = 4.8 cm * 2 = 9.6 Ksi

б2 = -5.35 cm * 2 = -10.7 ksi

ii) maximum in-plane shear stress

ζ  = radius of Mohr's circle

   = 5.1 cm = 10.2 Ksi   ( Given that ; 1 cm = 2Ksi )

iii) average normal stress

 = 9.6 + ( - 10.7 ) / 2

  = -0.51Ksi

Do you know who Candice is

Answers

Answer: Can these nuts fit in your mouth?

Explanation:

im just here for the points >:)

Draw a sinusoidal signal and illustrate how quantization and sampling is handled by
using relevant grids.

Answers

Answer: Your mums gay

Explanation: Your mums gay

A flow inside a centrifuge can be approximated by a combination of a central cylinder and a radial line source flow, giving the following potential function:
Ø= a2/r -cosØ + aßlnr = r
Where a is the radius of the central base of the centrifuge and ß is a constant.
a) Provide expressions for the velocities Vr and vo .
b) Find the expression for the stream function.

Answers

Answer:

a)  Vr = - a^2/r cosθ  + aß / r

    Vθ = 1/r [ -a^2/r * sinθ ]

b) attached below

Explanation:

potential function

Ø= a^2 /r  cosØ + aßlnr ----- ( 1 )

a = radius ,  ß = constant

a) Expressions for Vr and Vθ

Vr =  dØ / dr  ----- ( 2 )

hence expression : Vr = - a^2/r cosθ  + aß / r

Vθ = 1/r dØ / dθ ------ ( 3 )

back to equation 1

dØ / dr = - a^2/r sinθ + 0  --- ( 4 )

Resolving equations 3 and 4

Vθ = 1/r [ -a^2/r * sinθ ]

b) expression for stream function

attached below

ow Pass Filter Design 0.0/5.0 points (graded) Determine the transfer function H(s) for a low pass filter with the following characteristics: a cutoff frequency of 100 kHz a stopband attenuation rate of 40 dB/decade. a nominal passband gain of 20 dB, which drops to 14 dB at the cutoff frequency Write the formula for H(s) that satisfies these requirements:

Answers

Answer:

H(s) = 20 / [ 1 + s / 10^5 ]^2

Explanation:

Given data:

cutoff frequency = 100 kHz

stopband attenuation rate = 40 dB/decade

nominal passband gain = 20 dB

new nominal passband gain at cutoff = 14 dB

Represent the transfer function H(s)

The attenuation rate show that there are two(2) poles

H(s) = k / [ 1 + s/Wc ]^2  ----- ( 1 )

where : Wc = 100 kHz = 10^5 Hz , K = 20 log k = 20 dB ∴ k = 20

Input values into equation 1

H(s) = 20 / [ 1 + s / 10^5 ]^2

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