In the shotgun approach to whole-genome sequencing (shotgun sequencing), random DNA fragments of a chromosome are sequenced. The fragment sequences are then assembled into a continuous sequence that represents the DNA of the entire chromosome.
What are the steps in the shotgun approach to whole-genome sequencing?
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Answer 1

Nucleotides are the chemicals that make up DNA fragments. A sugar group, a phosphate group, and a nitrogen base make up each nucleotide.

WGS typically comprises six steps: library building, paired-end sequencing (PE sequencing), random fragmentation of the genomic DNA, size selection using electrophoresis, and genome assembly. The whole-genome shotgun (WGS) approach involves the parallel sequencing of a huge number of overlapping DNA fragments, which are subsequently stitched together by a computer to form bigger contigs and, eventually, chromosomes (Figure 1). Shotgun sequencing involves randomly slicing the genome into small, sequencing-ready pieces (1 to 2 kbp in length). After being ligated into an appropriate vector, the fragments are partly sequenced. In a single sequencing run, each fragment can provide 400–500 bp of sequence.

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Related Questions

Which of the following are abiotic factors that determine the organisms that can exist in marine environments?
O amount of oxygen and salt concentration
O amount of carbon dioxide and amount of decomposers
O amount of moisture and variety of consumers
O amount of algae and range of temperature

Answers

Answer:

O amount of moisture and variety of consumers

Abiotic factors include sunlight, temperature, moisture, wind or water currents, soil type, and nutrient availability. 

Which of the following PCR modifications was developed to allow for the amplification of an RNA template ? A ) Multiplex B ) Nested C ) Reverse transcriptase D ) Real - time

Answers

Transcriptase in reverse To enable the amplification of an RNA template, PCR modifications were created.

The relatively straightforward polymerase chain reaction (PCR) technology amplifies a DNA template to generate certain DNA fragments in vitro. Amplification of DNA sequences by PCR requires much less time than traditional procedures, which can take days or weeks to clone a DNA sequence into a vector and replicate it in a living cell.

Contrary to conventional biochemical tests, which include nucleic acid detection with radioisotopes, the PCR procedure only needs a little amount of biological material. As a result, PCR can amp up individual sequences at higher levels and with greater sensitivity than earlier techniques did.

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which of the following terms are used to describe the appearance of the edge or margin of a colony growing on an agar surface? A. Turbid B. Smooth C. Wavy D. Convex E. Irregular

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Irregular is the following terms are used to describe the appearance of the edge or margin of a colony growing on an agar surface.

1. Start by looking at the colony on the agar surface.

2. Observe the shape, size and color of the colony.

3. Pay close attention to the edge or margin of the colony.

4. Look for any irregularities in the shape, size and color of the edge or margin.

5. If any irregularities are present, the edge or margin of the colony can be described as irregular.

6. An agar surface is a type of laboratory media that is used to grow microorganisms. It is a gel-like substance composed of various nutrients and minerals.

7. When a colony of microorganisms is growing on an agar surface, the edge or margin of the colony can be observed.

8. The appearance of this edge or margin can be described as irregular. This means that the edge is not even or uniform in shape. It may have bumps, ridges, or other irregularities.

9. The irregular edge of a colony on an agar surface is usually caused by the growth of different types of organisms in the colony. This can make the edge appear uneven or irregular.

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T/F: Kelps with air bladders are common along the western and northwestern Pacific coasts of the United States

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Kelps with air bladders are common along the western and northwestern Pacific coasts of the United States it is a true statement

The primary moisture-bearing and rain-producing air mass throughout the year is the maritime Tropical (mT). It moves toward the pole in the winter and is cooled by the earth's surface. As a result, it has low stratus or stratocumulus clouds, drizzle, and poor visibility.

Due to the high-pressure areas that are prominent there, warm, humid maritime tropical (mT) air develops over tropical and subtropical ocean waters. On the other hand, just south and east of the substantial winter oceanic low-pressure regions, cool, moist maritime polar (mP) air develops over the colder subpolar ocean waters.

Humans have long been concerned with forecasting and predicting future weather conditions as a result of the variability of weather phenomena.

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The correct answer is True with air bladders are common along the western and northwestern Pacific coasts of the United States.

The middle intertidal zone is where the tides ebb and flow twice daily and where a wider range of flora and animals, such as sea stars and anemones, may be found. The intertidal zone is home to a variety of creatures, many of which have evolved to thrive in this continuously shifting environment. Mollusks (clams and mussels) and arthropods (crabs) have shells to prevent drying out and being crushed against the rocks by waves. TRUE: The majority of soft-bodied creatures and marine algae live in the low tide zone. The majority of creatures with shells are located in the spray zone.

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If a patient is having a hard time digesting a certain lipid known as triacylglycerol. What organ(s)
effect the digestion of this lipid? Why could this be happening?

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Triacylglycerol, also known as a fat molecule, is primarily digested by enzymes produced by the pancreas, called lipases. These enzymes break down the fat molecules into fatty acids and glycerol, which are then absorbed by the small intestine. The process of fat digestion also requires the presence of bile, which is produced by the liver and stored in the gallbladder. In some cases, a person may have difficulty digesting fats due to a lack of these enzymes or bile, which can be caused by conditions such as pancreatitis or gallbladder disease. Other factors that can affect fat digestion include certain medications, food intolerances, and diseases that affect the small intestine, such as Crohn's disease or celiac disease.

WILL MARK BRAINLIEST
The presence of the mineral iridium in Earth's rocks gives evidence to what events in Earth's history?

Question 3 options:

Solar storm activity


Increased volcanic eruptions


Asteroid impacts


Reversed polarity

Answers

The presence of the mineral iridium in Earth's rocks gives evidence to asteroid impacts. Option C

What is Iridium?

The element that is called iridium is one of the elements that are contained in the earth's crust. Many of the isotopes of iridium have been found to be radioactive. This is why we can be able to talk about iridium as we are looking at the history of the earth.

The mineral iridium is often present in large amounts and has been part of the geological history of the earth. It has taken part in all the earth events that have been taken place over the geological time scale of the earth and this explains why a study of the element is important as we are looking at the geological timescales of the earth in which we live and carry out activities.

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Besides increasing blood pressure and causing drinking, angiotensin II stimulates the release of ______, which is crucial to Na+ conservation

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Besides increasing blood pressure and causing drinking, angiotensin II stimulates the release of aldosterone.

A steroid hormone made by means of the adrenal cortex. It allows for managing the balance of water and salts within the kidney by using preserving sodium and liberating potassium from the frame. Too much aldosterone can purpose excessive blood strain and a build-up of fluid in body tissues.

In hyperaldosteronism, overproduction of the hormone aldosterone leads to fluid retention and improved blood pressure, weak point, and, rarely, periods of paralysis. Hyperaldosteronism can be a result of a tumor in the adrenal gland or can be a response to some illnesses.

Aldosterone reasons sodium to be absorbed and potassium to be excreted into the lumen through principal cells.

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There are two types of systems: open and closed. An open system is one in which energy can be transferred between the system and its surroundings. The stovetop system is open because heat can be lost into the air. A closed system is one that cannot transfer energy to its surroundings. Biological organisms are open systems.

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Biological organisms are open systems. Through the consumption of energy-storing molecules and the release of energy to the environment through labor, they exchange energy with their surroundings.

Energy is governed by the rules of physics, just like everything else in the physical universe. The movement of energy inside and between all systems in the universe is governed by the principles of thermodynamics. Cells are biological systems on their own. One way to think about systems is as having some degree of order open systems. A system needs energy to be brought into order. The lower the entropy, the more organized a Biological organisms.

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if tyrosine levels in a cns neuron were reduced, the synthesis of which of the following molecules might also be reduced

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If tyrosine levels in a CNS neuron were reduced, the synthesis of dopamine molecules might also be reduced.

Dopamine is a chemical ejected in the brain that causes euphoria. Having the appropriate amount of dopamine is essential both for your body and your brain. Dopamine assists nerve cells in communicating with one another.

Tyrosine is a nonessential amino acid the body produces from another amino acid called phenylalanine. It is an essential element for the production of many crucial brain chemicals known as neurotransmitters, which would include epinephrine, norepinephrine, and dopamine.

Because tyrosine aids in the production of the mood-altering chemical dopamine, and because individuals who are depressed frequently have low tyrosine levels, tyrosine can be given to treat depression

Tyrosine is thought to boost levels of the neurotransmitters dopamine, adrenaline, and norepinephrine. It may actually boost memory and effectiveness in stressful conditions by enhancing these neurotransmitters 

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Complete question :

If tyrosine levels in a CNS neuron were reduced, the synthesis of which of the following molecules might also be reduced?

A: beta-endorphins

B: acetylcholine

C: GABA

D: dopamine

which of the following represents the path taken by food after leaving the mouth? question 34 options: pharynx, esophagus, stomach, small intestine, large intestine pharynx, larynx, esophagus, small intestine, large intestine larynx, esophagus, stomach, small intestine, large intestine larynx, esophagus, stomach, large intestine, small intestine

Answers

The right answer is option a. Food first enters our bodies through the mouth before moving through the oesophagus and ending up in the stomach. After leaving the stomach, it moves into the small intestine and then the large intestine.

The GI tract is made up of multiple hollow organs that link to one another from the mouth to the anus. The hollow organs that make up the GI tract are the anus, small intestine, large intestine, and stomach. The part of your digestive process that receives food from your mouth is called the pharynx, which is located in your throat. The pharynx is connected to the oesophagus, which transports food to the stomach, and the trachea, also known as the windpipe, which transports air to the lungs.

So, we can conclude that option (a) is the appropriate response.

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The first detailed and quantitative studies on inheritance were carried out by an Austrian monk named _____. A. Mendel B. Darwin C. Mendeleev D. Benedict

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The first detailed and quantitative studies on inheritance were carried out by an Austrian monk named Gregor Mendel.

Mendel is known as the "father of genetics" for his pioneering work on the inheritance of traits in pea plants, which laid the foundation for the field of genetics. His work, published in the mid-1800s, showed that traits are inherited through the transmission of discrete units of information, now known as genes, from parents to offspring.

Mendel's work was largely ignored at the time, but it was rediscovered in the early 20th century and has had a profound impact on the understanding of how traits are passed down from generation to generation.

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please select all of the locations where coccidioidomycosis is endemic.check all that apply semi-arid desert areas semi-arid desert areas the western hemisphere the western hemisphere the south atlantic states of the united states the south atlantic states of the united states the mississippi and ohio river drainage areas of the united statesthe mississippi and ohio river drainage areas of the united states

Answers

The locations where coccidioidomycosis is endemic are a) Semi-arid deserts and b) The western hemisphere

Coccidioidomycosis is most common in the southwestern United States' semi-arid desert climate. Acute disease is brought on by the organisms being breathed in as spores, resulting in fever, chills, chest pain, hemoptysis, and dyspnea. The study of chest imaging reveals both condolidation and caitation, as well as widespread hilar lymphadenopathy. Patients with a previous infection are susceptible to reactivation and dissemination, regardless of their immunocompromised status. The semiarid regions of the United States have long been identified as the Coccidioidomycosi epidemic region, which is also located in the Western hemisphere. The activities that allow the fungus to enter the air and be inhaled by the susceptible host are the cause of the infection.

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Categorize the following neurotransmitter/neuromodulator as to its ef belong to more than one category fect on the postsynaptic neuron. Some neurotransmitters may GABA Excitatory Inhibitory Glutamate 00-59-47 Dopamine Glycine Norepinephrine Nitric Oxide Acetylcholine Substance P

Answers

A neurotransmitter is a signaling molecule secreted by way of a neuron to have an effect on any other cell across a synapse. The mobile receiving the sign, any major body element or goal cellular, can be any other neuron, but could also be a gland or muscle cell.

Excitatory Inhibitatory

Glutamate - Helps in learning and memory. GABA - Reduces the Activity of neurons.

Dopamine - When it binds to D1 receptors Dopamine - When it binds to D2 receptors.

Glycine Glycine

Norepinephrine - Raises heartbeat and alertness.  

Nitric Oxide - Increases blood flow to the brain and reduces oxidative stress there.  

Acetylcholine - Activates the motor neurons to move and control muscles.  

Substance P - Activates the vomiting center in the Medulla, regulates mood disorders and stress in the body.  

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The biological and biosocial theories seem to have the most immediate promise in dealing with problems of _____

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The biological and biosocial theories seem to have the most immediate promise in dealing with problems of postpartum depression.

People of both sexes may have postpartum depression (PPD), also known as postnatal depression, which is a type of mood disorder connected to childbirth. Extreme melancholy, low energy, concern, weeping spells, irritability, and changes in eating or sleeping habits are just a few symptoms. Between one week and one month is the typical postpartum onset period. PPD can be harmful to a newborn infant as well.

Although the precise origin of PPD is unknown, a confluence of biological, psychological, genetic, and social variables is probably to blame. These could include things like hormone adjustments and lack of sleep.

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are hermit crab affected by changes in water level (if it even is affected)​

Answers

Answer:

Hermit crabs are intertidal organisms, hermit crabs already possess strong adaptions to large fluctuactions in temperature, and are largely unaffected by it because they experience the changing conditions daily.

Explanation:

What is the main benefit of an upwelling in the ocean?
OA.
OB.
O C.
OD.
It creates strong tides in coastal regions.
It increases the salinity of the water in an area.
It brings new nutrients into an underwater region.
It wipes out an ecosystem, allowing for secondary succession.

Answers

Answer: C.

Explanation:

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A 21-year-old woman delivered a normal term infant a week ago and is now nursing the infant. She now notes a lump in her right axilla that has increased in size over the past week. On physical examination there is a rubbery, mobile, 1.5-cm mass beneath the skin at the right anterior axillary line. The mass is excised and the microscopic appearance is shown in the figure. Which of the following hormones most likely pro- duced the greatest effect upon this tissue?
A Cortisol
B Growth hormone
C Oxytocin
D Prolactin
E Testosterone

Answers

On physical examination there is a rubbery, mobile, 1.5-cm mass beneath the skin at the right anterior axillary line.

Oxytocin

The peptide hormone and neuropeptide oxytocin (Oxt or OT) is typically made in the hypothalamus and released by the posterior pituitary. It contributes to social interaction, reproduction, childbirth, and the time following childbirth. During labor and in response to sexual activity, the hormone oxytocin is released into the bloodstream. Additionally, it is accessible in pharmaceutical form. Oxytocin induces uterine contractions in both forms, hastening the labor process. In its natural state, it also contributes to the formation of the mother-baby relationship and milk production. A positive feedback loop governs the creation and release of oxytocin, with the first release of the hormone stimulating subsequent production and release. For instance, when oxytocin is released throughout a uterine contraction

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In which brain structure does short term memory information processing take place?
A. brainstem
B. medulla
C. hypothalamus
D. hippocampus

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In which brain structure does short term memory information processing take place hippocampus

A sophisticated brain structure located deep within the temporal lobe is the hippocampus. It plays a significant part in memory and learning. It is a malleable and delicate structure that is susceptible to various stimuli. According to studies, it is also impacted by a number of neurological and mental problems.What three activities does the hippocampus regulate?

The hippocampus, which is a crucial component of the limbic system, controls learning, memory encoding, memory consolidation, and spatial navigation.According to Schumacher et al. (2018), the hippocampus, which is part of the medial temporal lobe and connected to the amygdala, regulates emotional memory recall and regulation. It has improved functional connectivity with the anterior cingulate or amygdala during emotional regulation and recall of positive memory.

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Place the biochemical name and enzymatic activity corresponding to each complex within the respiratory chain. Complex 1 Complex II Complex III Complex IV Answer Bank cytochrome bo, complex facilitates electron transfer from ubiquinol to cytochromec facilitates electron transfer from cytochrome o to 0, facilitates electron transfer from spyinate to ubiquinone succinate dehydrogeonse complex facilitates electron transfer from NADH to ubiquinone cytochrome oxidase NADH dehydrogenase complex

Answers

The series of electron flow in the respiratory chain in the mitochondria membrane is-

NADH -------> Q --------> Cyt b ------> Cyt c1 --------> Cyt c -------> Cyt (a + a3) -------> O2

Complex I ; NADH dehydrogenase Complex Facilitated the electron transfer NADH to ubiquinone

Complex II; Succinate dehydrogenase Complex Facilitated the electron transfer succinate to ubiquinone

Complex III; Cytochrome bc1 complex or Ubiquinone: cytochrome c oxidoreductase Facilitated the electron transfer from ubiquinol to cytochrome c.

Complex VI; Cytochrome oxidase Facilitated the electron transfer from cytochrome c to O2​​​​​​.

In the electron transport chain, four protein complexes (designated complex I-IV) are involved in transporting electrons from NADH and FADH2 to molecular oxygen.

Complex I creates the hydrogen ion gradient by pumping four hydrogen ions from the matrix into the intermembranous region across the membrane.

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(50 points)Diurnal temperature range is the difference between ________ and ________ temperature each _________. high, low, hour high, low, day beginning, ending, hour beginning, ending, day Normal cells have a contact inhibition feedback mechanism that prevents the cellsfrom replicating when other cells are touched. A diagram of cells with contactinhibition and cells without contact inhibition is shown.Which statement best describes what will happen to cells in an organism that lackcontact inhibition?ANSWER CHOICESThe cells will stop replicating once one layer is produced as other body cells will crowd them out.The cells replicate into the food supply for each cell is used, causing each cell to die The cells will continue to grow causing a tumor in the body The cells will replicate normally replacing body cells that die A physics student walks 6 meters east, 4 meters north, then 9 meterswest. What is the student's magnitude of displacement from startingposition? Whereas hearing is a physiological process, attending is a ____________ one.a. psychologicalb. mediatedc. relationald. socialAnswer: a. psychological Hello can someone help me out with these two questions!?!?! This will help me a lot thank you! :D while investigating a case involving a stolen dog, police receive a tip about a woman on the street who has been seen trying to lure dogs into her yard with treats. the police go to the woman's house to speak to her. when she answers the door, police hear barking from the back of the house. they ask the woman to show them who is barking. before she can answer, the stolen dog bounds out of the kitchen toward the officers. they arrest the woman for theft and take her to the police station. immediately, officers begin to question the woman, who eventually confesses to stealing the dog. once she is charged, though, she pleads not guilty. her lawyer says that her confession does not matter because the police missed an important procedure during the case. what procedure did the police most likely forget? question 6 options: they forgot to confirm that a crime had occurred. they did not have probable cause to question the woman. they did not read the woman her miranda warning when arresting her. they did not offer the woman bail. question in screenshot (need help ASAP) alphabet company, which uses the periodic inventory method, purchases different letters for resale. alphabet had no beginning inventory. it purchased a thru g in january at $9.00 per letter. in february, it purchased h thru l at $11.00 per letter. it purchased m thru r in march at $12.00 per letter. it sold a, d, e, h, j and n in october. there were no additional purchases or sales during the remainder of the year. if alphabet company uses the weighted average method, what is the cost of its ending inventory? 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