Two different enzymes catalyze the same reaction and both exhibit the same Vmax. When Enzyme A was run with a 40 uM substrate, the initial rate (Vo) was 10 uM/min, and when g it was run with a 4 mM substrate, the Vo was 20 uM/min. Estimate the approximate Vmax and Km of Enzyme A. When Enzyme B was run with 80 uM substrate, the initial rate (Vo) was 10 uM per minute. Estimate the Km of Enzyme B.
Answer:
a) k_m = 4.08 uM
V_{max} = 20.07 uM/min
b) k_m = 8.16 uM
Explanation:
Given that:
For Enzyme A:
the substrate concentration [S] = 40 uM
the initial velocity rate v = 10 uM/min
when it was 4mM, v = 20 uM/min
i.e.
at 4mM = 4000 uM;
Using Michealis -menten equation;
when v = 10
[tex]V = \dfrac{V_{max}[S]}{k_m+[S]}[/tex]
∴
[tex]10 = \dfrac{V_{max}\times 40}{k_m + 40}[/tex]
[tex]10 (k_m + 40) = V_{max}40[/tex]
[tex]40V_{max} -10k_m = 400 --- (1)[/tex]
when v= 20
[tex]20= \dfrac{V_{max}\times 4000}{k_m + 4000}[/tex]
[tex]20 (k_m + 4000) = V_{max}4000[/tex]
[tex]4000V_{max} -20k_m = 8000 --- (2)[/tex]
equating equation (1) and (2):
[tex]40V_{max} -10k_m = 400 --- (1)[/tex]
[tex]4000V_{max} -20k_m = 8000 --- (2)[/tex]
let multiply equation (1) by 100 and equation (2) by 1
4000V_{max} - 1000K_m = 4000
4000V_{max} - 20 k_m = 8000
0 -980k_m = 4000
k_m = 4000/-980
k_m = 4.08 uM
replacing the value of k_m into equation (1)
40{V_max } - 10(4.08) = 400
40{V_max } - 40.8 = 400
40{V_max } = 400 + 40.8
40{V_max } = 440.8
V_{max} = 440.8/40
V_{max} = 11.02 uM/min
b)
Since V_{max} of A ie equivalent to that of B; then:
V_{max} of B = 11.02 uM/min
Here;
[S] = 80 uM
V = 10 uM/min
∴
[tex]10 = \dfrac{11.02 \times 80}{k_m + 80 }[/tex]
10(k_m +80) = 881.6
10k_m = 881.6 - 800
10k_m = 81.6
k_m = 81.6/10
k_m = 8.16 uM
Dehydration synthesis leads to WICH formation
Answer:
Polymers...........
Explanation:
what will most likely happen if a population is large and no migration, mutation, or environmental change occurs?
1. natural selection will increase
2. nonrandom mating will start to occur
3. the rate of evolution will increase
4. gene frequencies will remain constant
Science is concerned with explaining natural phenomena, based on observation, experiments, and evidence. Other questions are best answered by using other forms of knowledge and understanding, such as art, philosophy, or religion. Read each topic and select the topics for Science.
Select ALL that apply.
behavior of alligators
meaning of existence
black holes
deep-sea vents
the Aurora Borealis (Northern Lights)
ghosts, goblins, and other creatures in stories
Answer:
These:
Explanation:
behavior of alligators
black holes
deep sea vents
the Aurora Borealis
Any event or an occurrence that happens on its own without any human intervention is termed as a natural phenomena.
Natural phenomena harms the nature as well as life on earth.
Common examples of natural phenomena include thunder storms, earthquakes, landslides, volcanic eruptions, hurricane etc.
Some majestic natural phenomena include blood rain, Northern lights and luminous water in the world.
Natural phenomena can sometimes be destructive in nature and are therefore responsible for loss of lives and property.
It is almost impossible to predict the occurrence of any natural phenomena.
However, the negative outcome of natural phenomena can be managed with special disaster management operation
Thus, natural phenomena could only be observed and understood in science.
Learn more about natural phenomena, here:
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economic importance of mosquito larva list five
Explanation:
economic importance of mosquito larva are
Mosquito larvae grow by consuming microorganisms such as algae and microbes that decompose decaying plant material. Larval mosquitoes contribute to aquatic food chains by serving as food sources for many predators, including fish and birds.i hope it will help you
