Biology College

test binding of a transcription regulator on specific dna sequences, which of the following techniques could be used?

Answers

Answer 1

There are various techniques that can be used to test the binding of a transcription regulator on specific DNA sequences. These techniques are discussed below: Electrophoretic Mobility Shift Assay (EMSA): It is a technique that is used to analyze protein-DNA interactions. TThe correct option is (A).

In this technique, a DNA sequence that binds to a transcription factor of interest is labeled with a radioactive or fluorescent marker. The labeled DNA is mixed with the protein of interest and then electrophoresed. The protein-DNA complexes move more slowly than unbound DNA and can be visualized and quantified by autoradiography or fluorescence imaging. Surface Plasmon Resonance (SPR): This technique is used to study biomolecular interactions. SPR is based on detecting changes in the refractive index caused by the binding of molecules to a metal-coated sensor surface. The sensor surface contains ligands that specifically bind to the molecule of interest. When the molecule of interest binds to the ligands, the refractive index changes and is detected by a detector. Signal Amplification System (SAS): This is a technique that can be used to detect the binding of a transcription factor to DNA. It involves the amplification of the signal generated by the binding of a transcription factor to DNA. The signal is amplified by the binding of a secondary antibody to the primary antibody that is bound to the transcription factor. The secondary antibody is conjugated with an enzyme that catalyzes a reaction to produce a detectable signal.

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Note the full question is

which of the following techniques could be used? test binding of a transcription regulator on specific dna sequences.

A)  Electrophoretic Mobility Shift Assay (EMSA)

B) GC rich regions help anchor transcription factor binding

Related Questions

How does water flow?

Answers

water flows down due to gravity.
Water will always form a flat surface unless it is acted on by another force, such as wind. Objects can sink, float, or stay suspended in water. Air takes up space in water and will float to the top. In nature, water flows down due to gravity.

as characteristized in lecture, an oil spill is an example of: question 12 options: competition antagonism ammensalism commensalism

Answers

Antagonism can take one of two forms: competitive and non-competitive. Ketamine, on the other hand, is a non-competitive foe of the NMDA-glutamate receptor, whereas naloxone is a competitive foe of all opioid receptors. The correct answer is Antagonism.

Competitive antagonism is based on the idea that an agonist or antagonist can bind to the same recognition site(s) on the receptor and compete for those sites when they are present simultaneously.

A competitive antagonist blocks the agonist's action by binding to the same site as the agonist but not activating it. To prevent the receptor from being activated, a non-competitive antagonist binds to an allosteric (non-agonist) site on the receptor.

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