The correct sequence for western blotting is:

1. Proteins are denatured by sodium dodecyl sulfate (SDS), and by boiling.
2.Transfer proteins to the nitrocellulose membrane.
3. Performing the polyacrylamide gel electrophoresis (PAGE).
4. Incubate the nitrocellulose membrane to primary antibodies and secondary antibodies subsequently.

a. 1,2,3,4
b. 4,1,3,2
c. 1,3,2,4
d. 1,3,2,4

Answers

Answer 1

Answer:

3.page is this that answer

Explanation:

a.1,2,3,4

Answer 2

The correct sequence for western blotting is: C. 1,3,2,4

Blotting can be defined as an analytical technique that is designed for the analysis of biological macromolecules such as:

Lipids.Nucleic acid.Proteins.Carbohydrates.

For western blotting, the correct sequence of analyzing biological macromolecules such as proteins is:

1. The denaturation of protein through the use of sodium dodecyl sulfate (SDS) and by boiling.

2. The performance of polyacrylamide gel electrophoresis (PAGE).

2.The transfer of proteins to the nitrocellulose membrane.

4. Lastly, the incubation of the nitrocellulose membrane to subsequently form primary antibodies and secondary antibodies.

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Related Questions

which type of soil is likely to be found in horizon E

Answers

Answer:

A layer of pale,Sandy soil lacking clay and iron is likely to be found in horizon E

Answer:

bedrock

Explanation: A layer or bedrock is the type of soil is likely to be found in horizon E. Hence the correct answer is option A among the options. The bed rocks can be regarded very hard and it cannot be breakable.


Evidence without_______
increases its reliability.
a
bias
b
question
с
repetition
d
replication

Answers

Answer:

I'll say its B ) Question

Explanation:

because when your proving something with evidence then you start asking questions (or still have questions) makes it seem like you don't really know.

A red flower producing snapdragon plant is crossed with a white flower producing snapgragon plant. Resulting F1 generation is crossed with one another to produce F2 generation.

This is incomplete dominance of genes.

Q1 What type of a breeding is this? (monohybrid, dihybrid or interspecific)

Q2 Draw a genetic chart to show P, F1 and F2 generations clearly indicating genotypes, phenotypes and generations.



Answers

Answer:

See the answer below

Explanation:

1. This is an example of monohybrid breeding. A monohybrid breeding is the type of breeding that involves parents with a pair of contrasting characters. On the other hand, a type of breeding involving a single gene is what is known as monohybrid breeding.

2. When a red flower snapdragon is crossed with a white flower snapdragon, the resulting offspring are usually pink - an indication of incomplete dominance of the gene responsible for flower color. Assuming the red flower's genotype is AA and that of the white flower is aa:

                       AA      x      aa

                 Aa      Aa      Aa      Aa

F1 genotype = all Aa

F1 phenotype = pink flower

At F2:

                          Aa      x      Aa

                 AA         2Aa           aa

F2 Genotype/phenotype:

       1AA - red color

       2Aa - pink flower color

        1 aa - white flower color.

Which is incorrect descriptions of the genetic event initiated by the HIV reverse transcriptase (RT) upon the HIV infection?
a. RT catalyzes initial synthesis of ssDNA using viral genomic RNA as the template.
b. The first RNA template is degraded after ssDNA synthesis.
c. The whole process is completed after synthesis of dsDNA.
d. tRNAs are adopted as the first primers.
e. None of these

Answers

Answer:

e. None of these

Explanation:

The immune deficiency viruses (HIV) are retroviruses that use a reverse transcriptase (RT) enzyme to produce a single-stranded DNA (ssDNA) from an RNA template. The reverse transcription allows retroviruses to replicate their genetic material, which is integrated into the host's genome as a double-stranded linear DNA molecule in a similar way to the mechanism of insertion used by endogenous retrotransposons. The synthesis of DNA is started by cellular tRNAs (tRNA3Lys) that are packaged into the virion. After reverse transcription, the HIV DNA enters the nucleus of CD4 immune cells (also known as CD4+ T cells), and then it integrates into the genome to coopt the host's cell machinery for its own replication.

Which of the following is NOT a factor of sustainability?
Group of answer choices

economics

ethics

biodiversity

natural capital

solar energy

Answers

Answer:

natural capital

HOPE IT HELPS!

PLS MARK AS BRAINLIEST!!!

The levels of nitrogen and ___ in the atmosphere remain fairly constant but the levels of other gases may vary.

Answers

Answer:Oxygen

Explanation:

1. A star is 520 light years from Earth. During what event in history did the
light now arriving at Earth leave the star?

Answers

Answer:

A light year is the distance which is equal to 9,460,730,472,580.8 km, so:  

= 4.91957985 X [tex]10^{15}[/tex]km  

which is distance travels by the light.  Now what time it takes light to travel distance we found.  

A year has 365.25 days, so,

[tex]1 (\frac{365.25)}{1 year}) (\frac{24}{1 day}) (\frac{3600 s}{1 hr} )[/tex] = 31557600 seg/year

The light speed in the space is equal to 299,792.458 km/s, so:  

4.91957985 x [tex]10^{15} (\frac{1 seg}{29792.458}) \frac{1 year}{31557600}[/tex] = 520 years

if today, August, 2020, then

2020 - 520 = 1500

Spanish and Portuguese spread out over the southern part of the Western Hemisphere and bring in America brought to Spanish colony of Santo Domingo in year 1500.

Therefore, during the  period 1499 AD Columbus discovered JamaicaA light-year is a distance traveled by light in space during a period of one year from a celestial object to another celestial object. The distance between stars and Earth is 520 light-years. So, the light leaving the star is 520 years ago. The present year is 2019. Light from the star left 520 years ago. The time period on Earth is,

[tex]t=2019-520\\ t=1499 AD[/tex]

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Which is a feature of a tonic receptor? Select one: a. The action potential occurs when there is a change in response to a change in condition. b. For this receptor, the stimulus begins with a burst of action potentials. c. They are normally inactive. d. When the stimulus changes the action potential generation changes. e. Provides information about the rate and change of the stimulus. f. The action potential is generated for a short time period.

Answers

Answer:

For this receptor, the stimulus begins with an explosion of action potentials.

that would be the correct option.

Explanation:

A tonic receptor is one that is activated when the action potentials were maintained over time and during the signaling of the receptor.

Tone receptors require continuous stimulation over a period of time to trigger a response and deliver it to the central nervous system.

It keeps the nervous system constantly active in the environment that surrounds it.

They are slowly adaptable, an example of these receptors are the merkel and ruffini receptors.

As a substance is eaten, trace its path through the digestive system. Include one of the basic processes each step of the way: digestion, absorption, motility, secretion, and excretion.

Answers

Answer:

....... motility

Explanation:

4
THE EAR
4.1
4.1.1 Which ONE of the following is a part of the ear where grommets
are inserted?
A Oval window
B Semi-circular canal
C Tympanic membrane
Pinna
noreen with middle ear infection is usually advised not to fly in an

Answers

Answer:

C

Explanation:

Zlrozdzidkzzkxhkcgkxtkckgcgkckzxtixkgx

what are biogeochemical ?​

Answers

Biogeochemical cycle, any of the natural pathways by which essential elements of living matter are circulated. The term biogeochemical is a contraction that refers to the consideration of the biological, geological, and chemical aspects of each cycle.

Without this, many cycles such as the water cycle and photosynthesis would not exist. What could all these cycles not exist without?

Answers

Answer:

This question appears incomplete

Explanation:

This question appears incomplete. However, one similar substance that, if missing, many cycles (particularly the two cycles/processes provided in the question) will not exist/proceed is the sun/sunlight.

In the water cycle for instance, if there is no sun/sunlight, there will be no heat to allow for evaporation of water from the water-body (ocean, sea, stream or lake) hence there will be no cloud of water droplets in the atmosphere. The implication of this is that, the first process of the water cycle will not proceed, hence the cycle will not exist.

During photosynthesis, carbondioxide reacts with water in the presence of sun/sunlight to produce glucose and oxygen. The absence of sun in this reaction will not lead to the production of glucose which is the useful product of photosynthesis for plant.

From the explanation above, it can be deduced that the absence of the sun/sunlight will prevent the two cycles from existing.

Under normal conditions glomerular filtration depends on three main pressures. What pressures is a pressure that favors the process of filtration?

Answers

Answer:

Glomerular Hydrostatic pressure .

Explanation:

The basic function of the kidney is the formation of urine for elimination through the urinary excretory system. Two different processes determine this formation: the filtration of fluid through the glomerular capillaries into Bowman's space and the modification of the volume and composition of the glomerular filtrate in the renal tubules.  The fluid passes from the glomerular capillaries to Bowman's capsule due to the existence of a pressure gradient between these two areas. This process is favored by two structural characteristics that make renal corpuscles particularly effective filtration membranes: glomerular capillaries have a much higher number of pores than other capillaries, and the efferent arteriole has a smaller diameter than the afferent arteriole, causing greater resistance to outflow of blood flow from the glomerulus and increasing glomerular hydrostatic pressure. Increased glomerular hydrostatic pressure (due to increased blood flow through the glomerulus) increases filtration, while increases in Bowman's hydrostatic pressure or urinary space (which remains constant, unless there is disease at that level, usually due to fibrosis) and plasma P. oncotic (determined by proteins, which tend to "drag" plasma into the glomerulus) decrease filtering. Resulting in a filtering pressure of 10 mmHg.

According to kirk smith a professor of environmental health at the university of california berkley indoor fires increase risks of pneumonia tuberculosis lung cancer and low birth weight in babies born of women expose during pregnancy. What simple solution is being widely promoted to reduce the risk of death?
a) preparing meals using solar cookers.
b) switching from wood to burning crop waste as a fuel source.
c) adding more windows to houses as a source of ventilation.
d) passing a green tax to make homeowners pay for their pollution.
e) providing asthma inhalers to children under the age of 12 years.

Answers

Answer:

preparing meals using solar cookers.

Explanation:

solar cookers  radiates at low rates.Therefore the most of the nutrients in the foods are conserved.Thus most micro nutrients for biochemical activities are retained. Vitamins which can not withstand high temperature are preserved.

Most importantly carcinogens which are usually associated with high heat foods are avoided ,when cook with low heat of solar cookers

The solar cooker is smoke free,therefore irritation of the lungs,lung cancer associated with high heat  cookers is avoided.

Specifically,local Mayan women exposed to high heat smoke cookers, suffers lung cancer,and those with pregnancy gives to infants with low birth weights. Children exposed to theses high heat also experienced acute lower respiratory infections.

Thus the smokeless,low heat solar cooker is safer.

whats the biggest mystery about the virus​

Answers

Explanation:

Hey, there!!

According to what i have learned, the biggest mystery about virus is; It acts like both living and non- living beings.

living characters includes :

It reproduce to make more no. of themselves. It feeds on various substances.

non- living characters:

It can be crystallize. It dont respire and feed eith it is out from a host body.

Hope it helps...

​"Acidic" is an appropriate description for four of the following. Which one is the exception?

A). Soup and ammonia

B). HCI

C). Excess hydrogen ions

D). The contents of the stomach

E). A pH less then 7

Answers

Answer:

E) A pH less than 7

Explanation:

if a solution has a higher concentration of hydronium ions than pure water , it has a pH lower than 7

Answer: e

Explanation:

I did the test

what is sexual reproduction

Answers

Answer:

See explanation below...

Explanation:

Sexual reproduction is a type of reproduction that involves a complex life cycle in which a gamete with a single set of chromosomes combines with another to produce an organism composed of cells with two sets of chromosomes.

Best Regards!

Answer:

the production of new living organisms by combining genetic information from two individuals of different types (sexes). In most higher organisms, one sex (male) produces a small motile gamete which travels to fuse with a larger stationary gamete produced by the other (female).

Which of the following properties is the temperature at which a liquid turns to gas? (3 points)
оа
Magnetism
Ob
Thermal conductivity
ос
Melting point
Boiling point
Od

Answers

thermal conductivity

Answer:

Boiling point

Explanation:

I did the test

What cell feature is used by scientists to classify an unknown cell as prokaryotic or eukaryotic?

Answers

Answer:

Like a prokaryotic cell, a eukaryotic cell has a plasma membrane, cytoplasm, and ribosomes, but a eukaryotic cell is typically larger than a prokaryotic cell, has a true nucleus (meaning its DNA is surrounded by a membrane), and has other membrane-bound organelles that allow for compartmentalization of functions.

Explanation:

Hope this helps ;)

A purebred tall pea plant is cross-pollinated with a tall, heterozygous pea plant. Use a Punnett square to determine the probability the offspring inherita
recessive short allele. (I point)
75%
25%
0%
50%

Answers

Answer:

0%

Explanation:

This question involves a gene coding for height in pea plants. The allele for tallness (T) is dominant over the allele for shortness (t). This means that allele T will be expressed over allele t in an heterozygous state.

A purebred tall plant will possess genotype: TT while a heterozygous tall plant will possess genotype: Tt. The two parents will produce the following gametes:

TT- T and T

Tt- T and t

Using these gametes in a punnet square (see attached image), the following offsprings with genotypes: TT and Tt in a ratio 1:1 will be produced.

TT offsprings are purebreed tall while Tt offsprings are heterozygous tall. Hence, based on the question, no offsprings of this cross will possess the recessive genotype (tt). This means that 0% of the offsprings of this cross will be short.


Which of the following groups gets energy directly from the grass it eats?

Answers

Answer:

herbivores

Explanation:

i think it's herbivores because "herb"ivores get energy when eating grass or any other herb.

if a short sequence of dna reads 3'TAACGTCCAGGCAAA5', what is the complementary sequence in the other strand of dna g

Answers

Answer:

5' ATTGCAGGTCCGTTT 3'

Explanation:

Complimentary strands of DNA run anti-parallel to each other, the ends facing in opposite directions.

The complimentary base pairs for DNA are:

A=T and C=G and when finding the complimentary strand these pairs are only paired with each other.

Question 3 (1 point)
During DNA replication, one of the new strands of DNA is synthesized continuously.
The other strand is synthesized as a number of separate fragments of DNA that are
subsequently linked by DNA ligase. Why does this occur?

- RNA primers only anneal to one of the parental strands of DNA.

- DNA polymerase III only synthesizes DNA in the 3' - 5' direction.

- DNA polymerase III only synthesizes DNA in the 5'-3' direction.

- One of the parental strands is unwound slower than the other by helicase.

Answers

Answer:

DNA polymerase III only synthesizes DNA in the 5'-3' direction.

Explanation:

DNA replication is an important phenomenon for every living cell. It is the process whereby the double-stranded DNA is doubled to form two new separate double strands. In order for DNA replication to occur, the double strand of the DNA molecule must first be unwound by an enzyme called DNA HELICASE. This gives two separate single strands, which individually acts as a template for the newly synthesized strands.

DNA polymerase III is the enzyme responsible for synthesizing new DNA strand by pairing complementary nucleotides to the old strands it attaches to. However, one of the old strands called LEADING STRAND runs in the 3'-5' direction while the other strand called LAGGING STRAND runs in the 5'-3' direction.

DNA polymerase III only attaches to the 3' hydroxyll end of the DNA and synthesizes new strand of DNA in the 5'-3' direction. Since the lagging strand runs in 5'-3', it is synthesized in small separate fragments called OKAZAKI FRAGMENTS which are later joined together by an enzyme called LIGASE.

N.B: As DNA polymerase synthesizes DNA strand on the leading strand(5'-3'), it is moving in an opposite direction of the lagging strand. Hence, it has to detach and come back to synthesize on the lagging strand. This causes the lagging strand to be synthesized discontinuously.

C. If you combined the elements in the product of this reaction, what type of

reaction would it be? Hint: What is the reverse of the equation you just

balanced? (1 point)

Answers

Answer:

Reversible reaction

Explanation:

If the elements of the product combine together in a chemical reaction, this reaction is called reversible reaction because the reaction moves in backward  or reverse direction. For example, if acid i. e. HCl combine with base i. e. NaOH which are the reactants, it produces two products salt (NaCl) and water (H2O). If salt (NaCl) and water (H2O) combine again with each other, it produces acid i. e. HCl and base i. e. NaOH, such type of reaction is called reversible reaction.

Which location is least likely to experience a volcanic eruption? Α. an island hot spot, such as the island of Hawaii B. Hamilton County on the plains of central Texas с. a convergent boundary, as in the Ring of Fire D a volcanic island arc, such as the Aleutian Arc in Alaska

Answers

Answer:

i think that the answer is B. Hamilton County on the plains of central Texas i took the test

Explanation:

Hamilton County on the plains of central Texas is least likely to experience a volcanic eruption. Therefore, option (B) is correct.

What are volcanoes?

Molten rock and gases stored under the surface erupt through a volcano, generating a hill or mountain.

Active, inactive, or extinct volcanoes. Active volcanoes are likely to erupt again. Dormant volcanoes may erupt again. Extinct volcanoes won't erupt.Magma collects inside active volcanoes. The magma chamber's pressure forces it through rock channels and onto the planet's surface.

Volcanic eruptions can be violent or slow-moving. Volcanoes erupt through vents on the sides or a primary entrance at the top. The volcano's morphology depends on eruption rate and magma chemistry. Land and sea volcanoes exist. As lava cools and hardens, underwater volcanoes build mountains and ranges. When volcanoes rise above the ocean, they create islands.

Learn more about volcano, here:

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The medical term ____________________ describes a pus-filled lesion on the eyelid resulting from an infection in a sebaceous gland.​

Answers

Answer:

the medical term is hordeolum

What is the wavelength of an earthquake wave if it has a speed of 10 KM/S and a frequency of 5HZ?

Answers

Answer:

2nm

Explanation:

(10KM/S)/(5HZ) = 2nm

The most abundant element in the body of living things is: a) Hydrogen b) Nitrogen c) Carbon d) Oxygen​

Answers

Answer:

D. Oxygen

Explanation:

Oxygen is the most abundant element in the bodies of living things. For example, oxygen is about 65% of the human body.

Oxygen in the body is mostly in the form of water, since the bodies of living things need water to create energy needed every day.

Why do you think premenopausal women need more iron than
men of the same age?

Answers

Answer:

Women need more iron than men to make up for the amount of iron they lose in their menstrual period.

Explanation:

Answer:

Premenopausal women shed blood as part of menstruation every month, which lowers the level of iron in the body. So, they need more iron than men and are also at a greater risk for this nutritional deficiency.

PLATO

Explanation:

what type of molecule do plant cells use for long term energy storage

Answers

Answer:

ATP

Explanation:

In plants, energy is stored in the form of ATP and NADPH. Energy is produced in the presence of light it is in the thylakoids and mitochondria.

ATP: Adenosine triphosphate

NADPH:  nicotinamide adenine dinucleotide phosphate hydrogen

Other Questions
In high school, a teacher gave two sections of a class the same arithmetic test. The results were as follows: Section I: Mean 45, Standard Deviation 6.5 Section II: Mean 45, Standard deviation 3.1 What conclusions is correct? The smartest thing a firm involved in an oligopoly market could do is to cut their prices and capture more of the market share from their competitors. a) We learned in class that the best move would be to raise prices. b) We also learned that cutting prices on an elastic demand curve will be a smart way of getting more revenues. c) Cutting prices is no gaurantee of success. Indeed if the firm does capture more market share and customers, then their costs will go up and it will be harder for them because they will have lower profit margins - if they can earn any profit at all. d) Both A and C are correct. h(x+1)=x^2-4 evaluate function by substuting x+1 How would removing invasive species from anecosystem help reduce the chances of a nativespecies becoming extinct?AThe native species would go extinctsooner.BThe native species would no longer beinfected by diseasesCThe native species would be better adaptfor survival.DThe native species would not have tocompete for necessary resources, Read this line from James Joyce's "Araby." Based on the context in which it appears, identify the correct synonym of the word amiability. I watched my master's face pass from amiability to sternness; he hoped I was not beginning to idle When HCl is added to water, the [H3O+] = 0.6 M. What is the [OH-]?What is the pH of the solution? The strategic appeal of related diversification is that it Multiple Choice allows a firm to reap the competitive advantage benefits of skills transfer, lower costs (due to economies of scope), cross-business use of a powerful brand name, and/or cross-business collaboration in creating stronger competitive capabilities. is less capital intensive than unrelated diversification because related diversification emphasizes getting into cash cow businesses (as opposed to cash hog businesses). involves diversifying into industries having the same kinds of key success factors. is less risky than unrelated diversification because it avoids the acquisition of cash hog businesses. facilitates the achievement of greater economies of scale since the company only enters those businesses that serve the same types of buyer groups and/or buyer needs. Solve for x. PLZ ANSWER FAST A list of financial statement items for Splish Brothers Inc. includes the following: accounts receivable $30,100, prepaid insurance $5,590, cash $22,360, supplies $8,170, and debt investments (short-term) $17,630.Prepare the current assets section of the balance sheet listing the items in the proper sequence. (List current assets in order of liquidity.) Is the following relation a function? (1 point) x y 1 2 2 3 3 1 6 2 No Yes Chapter 61. What is one theme you are seeing emerge in the novel A long walk to water so far? Explainyour answer. It takes a graphic designer 1.5h to make one page of a website. Using a new software, the designer could complete each page in 1.25h, but it takes 8h to learn the software. How many web pages would the designer have to make in order to save time using the new software? In the Assembly Department of Hannon Company, budgeted and actual manufacturing overhead costs for the month of April 2017 were as follows.Budget ActualIndirect materials $14,200 $13,700Indirect labor 19,100 19,900Utilities 11,400 12,100Supervision 4,600 4,600All costs are controllable by the department manager.Prepare a responsibility report for April for the cost center. (c) State two differences between this mesophyll cell and a root hair cell. (2 marks) A red flower producing snapdragon plant is crossed with a white flower producing snapgragon plant. Resulting F1 generation is crossed with one another to produce F2 generation. This is incomplete dominance of genes.Q1 What type of a breeding is this? (monohybrid, dihybrid or interspecific)Q2 Draw a genetic chart to show P, F1 and F2 generations clearly indicating genotypes, phenotypes and generations. Why do people say that some stuff is weird when no one and nothing is normal? Please solve.Essie likes plants. She never misses a chance to go to the nursery section in Home Depot. Essie has a total of $65.50 to spend on her trips to the store over the next three days. On her second trip to the nursery she spent $15.50 dollars more than what she spent on the first trip to the store. On her third trip to the nursery she spent two times much as what she spent on her first trip to the nursery. How much did she spend on her first trip to the nursery? Create a linear equation to model a real-world situation and solve the equation to find the solution The roller coaster car reaches point A of the loop with speed of 20 m/s, which is increasing at the rate of 5 m/s2. Determine the magnitude of the acceleration at A if pA to produce a tiny of the color green, you should add A,whiteB,blackC,a tertiary colorD,a secondary color What is xI dont get it