Weigh out the appropriate amount of HEPES sodium salt required to prepare 100 mL of a HEPES buffer that has pH 7.50 and is 90 mM in HEPES. FW for the HEPES sodium salt is 260.31. Transfer to a 150 mL beaker. Flowchart: Calculate the amount of HEPES sodium salt required to make the buffer.

Answers

Answer 1

Answer:

2.343 g of HEPES sodium salt is needed.

Explanation:

So, from the question above we have the following parameters which is going to help in solving this particular question.

The number of moles = 90mM, the pH = 7.50, FW for the HEPES sodium salt = 260.31, and the volume of HEPES sodium salt required = 100mL.

Therefore, the amount of HEPES sodium salt required to make the buffer = 260.31 × 9 × 0.001 = 2.343 grams of HEPES sodium salt is needed in 10mL.


Related Questions

True or false: potential energy increases as like charges get closer to one another

Answers

True, is the correct answer.

how will you ensure your server is protected from data hacks?

Answers

Close all network ports, filter those you can't block

Firewalls are used to filter network traffic and are available as standard system software on most operating systems. Limit the openings hackers have to your server.

Answer:

Establish and Use a Secure Connection

Use SSH Keys Authentication

Secure File Transfer Protocol

Secure Sockets Layer Certificates

Use Private Networks and VPNs

Explanation:

What is the correct name of CUCIO2
Chemistry

Answers

Answer:

Copper(II) Chlorite = Cu(ClO2)

Explanation:

how you write the compound is very important as well

A mixture is made by combining 1.29 lb of salt and 5.35 lb of water.
What is the percentage of salt (by mass) in this mixture?

Answers

Answer:

19.47%

Explanation:

[tex]\frac{1.29}{1.29+5.35}[/tex]

Please recheck, for this answer may not be correct

the reaction between potassium metal and water produces potassium hydroxide and hydrogen gas.

the correct equation for this reaction is:

A: K + H2O—> KOH + H2
B: K + H2O—>KOH + H
C: 4K + 3H2O—> 4KOH + 2H
D: 2K + 2 H2O—> 2KOH + H2

Answers

Answer:

2K   +   2H₂O   →   2KOH  + H₂

Explanation:

The reactants are:

    Potassium metal  = K

    Water  = H₂O

 The products are:

  Potassium hydroxide  = KOH

  Hydrogen gas  = H₂  

The reaction equation is given as;  

                Reactants   →   Products

        2K   +   2H₂O   →   2KOH  + H₂

The reaction is a single displacement reaction

What is the molar mass of Mg(CIO3)2

Answers

the molar mass is 191.2 g/mol

Which macromolecules break apart by hydrolysis?

Answers

Answer:

Dehydration synthesis reactions build molecules up and generally require energy, while hydrolysis reactions break molecules down and generally release energy. Carbohydrates, proteins, and nucleic acids are built up and broken down via these types of reactions, although the monomers involved are different in each case.

Explanation:

Magnesium and Copper Sulfate react to form Magnesium Sulfate and Copper in the following equation.
Mg + CuSO4 -> MgSO4 + Cu

Answers

Answer:

B: Single Displacement Reaction

Explanation:

A single replacement reaction, sometimes called a single displacement reaction, is a reaction in which one element is substituted for another element in a compound.

A + BX -> B + AX

For example:

Cation Replacement

Zn + CuCl2 -> ZnCl2 + Cu

Anion Replacement

Br2 + 2Ki -> 2KBr + I2

You just switch either the cation with another anion or switch an anion with another cation.

Hope it helped!

Based on the molecular formula, determine whether each of the following is an alkane, alkene, or alkyne. (Assume that the hydrocarbons are noncyclical and there is no more than one multiple bond.)
a. C3H6
b. C6H12
c. H8H18
d. C7H12

Answers

Answer:

C₃H₆ is an alkene

C₆H₁₂ is an alkene

C₈H₁₈ is an alkane

C₇H₁₂ is an alkyne

Explanation:

To determine which of the compound is alkane, alkene, or alkyne,we shall use the general formula of alkane, alkene, and alkyne. This is illustrated below:

General formula for alkane => CₙH₂ₙ₊₂

General formula for alkene => CₙH₂ₙ

General formula for alkyne => CₙH₂ₙ₋₂

For C₃H₆:

n = 3

Alkane => CₙH₂ₙ₊₂ => C₃H₂₍₃₎₊₂ => C₃H₈

Alkene => CₙH₂ₙ => C₃H₂₍₃₎ => C₃H₆

Alkyne => CₙH₂ₙ₋₂ => C₃H₂₍₃₎₋₂ => C₃H₄

Thus, C₃H₆ is an alkene

For C₆H₁₂:

n = 6

Alkane => CₙH₂ₙ₊₂ => C₆H₂₍₆₎₊₂ =>C₆H₁₄

Alkene => CₙH₂ₙ => C₆H₂₍₆₎ => C₆H₁₂

Alkyne => CₙH₂ₙ₋₂ => C₆H₂₍₆₎₋₂ => C₆H₁₀

Thus, C₆H₁₂ is an alkene

For C₈H₁₈:

n = 8

Alkane => CₙH₂ₙ₊₂ => C₈H₂₍₈₎₊₂ => C₈H₁₈

Alkene => CₙH₂ₙ => C₈H₂₍₈₎ => C₈H₁₆

Alkyne => CₙH₂ₙ₋₂ => C₈H₂₍₈₎₋₂ => C₈H₁₄

Thus, C₈H₁₈ is an alkane.

For C₇H₁₂:

n = 7

Alkane => CₙH₂ₙ₊₂ => C₇H₂₍₇₎₊₂ => C₇H₁₆

Alkene => CₙH₂ₙ => C₇H₂₍₇₎ => C₇H₁₄

Alkyne => CₙH₂ₙ₋₂ => C₇H₂₍₇₎₋₂ => C₇H₁₂

Thus, C₇H₁₂ is an alkyne.

SUMMARY:

C₃H₆ is an alkene

C₆H₁₂ is an alkene

C₈H₁₈ is an alkane

C₇H₁₂ is an alkyne

What is ATP? How does it provide energy

Answers

Did you try to search it up?

How many molecules are in 20,484 grams of H2O?​

Answers

Answer:

3.4027x10^-22

Explanation:

The following graph shows a decay chain. Which part of the decay chain will take the most time?

Answers

I do not see a chart but I would say for what ever has been there the longest and what had more ability to withstand their environment ;) hope I helped

A 0.4647-g sample of a compound known to contain only carbon, hydrogen, and oxygen was burned in oxygen to yield 0.8635 g of CO2 and 0.1767 g of H2O. What is the empirical formula of the compound?

Answers

Answer: The empirical formula is [tex]C_3H_3O_2[/tex].

Explanation:

The chemical equation for the combustion of hydrocarbon having carbon, hydrogen and oxygen follows:

[tex]C_xH_yO_z+O_2\rightarrow CO_2+H_2O[/tex]

where, 'x', 'y' and 'z' are the subscripts of Carbon, hydrogen and oxygen respectively.

We are given:

Mass of [tex]CO_2[/tex] = 0.8635

Mass of [tex]H_2O[/tex]= 0.1767 g

Molar mass of carbon dioxide = 44 g/mol

Molar mass of water = 18 g/mol

For calculating the mass of carbon:

In 44g of carbon dioxide, 12 g of carbon is contained.

So, in 0.8635 g of carbon dioxide, =[tex]\frac{12}{44}\times 0.8635=0.2355g[/tex] of carbon will be contained.

For calculating the mass of hydrogen:

In 18g of water, 2 g of hydrogen is contained.

So, in 0.1767 g of water, =[tex]\frac{2}{18}\times 0.1767=0.0196g[/tex] of hydrogen will be contained.

Mass of oxygen in the compound = (0.4647) - (0.2355+0.0196) = 0.2096 g

Mass of C = 0.2355 g

Mass of H = 0.0196 g

Step 1 : convert given masses into moles.

Moles of C =[tex] \frac{\text{ given mass of C}}{\text{ molar mass of C}}= \frac{0.2355g}{12g/mole}=0.0196moles[/tex]

Moles of H=[tex]\frac{\text{ given mass of H}}{\text{ molar mass of H}}= \frac{0.0196g}{1g/mole}=0.0196moles[/tex]

Moles of O=[tex]\frac{\text{ given mass of O}}{\text{ molar mass of O}}= \frac{0.2096g}{16g/mole}=0.0131moles[/tex]

Step 2 : For the mole ratio, divide each value of moles by the smallest number of moles calculated.

For C =[tex]\frac{0.0196}{0.0131}=1.5[/tex]

For H =[tex]\frac{0.0196}{0.0131}=1.5[/tex]

For O = [tex]\frac{0.0131}{0.0131}=1[/tex]

The ratio of C : H: O = 1.5 : 1 .5 : 1

Converting to simple whole number ratio:

The ratio of C : H: O = 3 : 3: 2

Hence the empirical formula is [tex]C_3H_3O_2[/tex].

What happens to water when it changes to ice

Answers

Answer:The atoms cool down and become slower and their bonds become close and they move less.

Explanation:I took science

Name the type of chemical reaction that occurs when magnesium chloride solution reacts with sodium carbonate solution

Answers

Answer:

precipitation reaction

The  replacement reaction occurs when magnesium chloride solution reacts with sodium carbonate solution and form [tex]MgCO_{3}[/tex] white precipitate.

What is  replacement reaction?The reaction in which cation of one molecule is replace or substitute by cation of another molecule or anion of one molecule is replace or substitute by another molecule.Example : [tex]MgCl + NaCO_{3}[/tex] → [tex]MgCO_{3} + NaCl[/tex]What is precipitate?The insoluble substance accumulate at the bottom of the surface is called precipitate.Example : [tex]MgCl + NaCO_{3}[/tex] → [tex]MgCO_{3} + NaCl[/tex]In the above reaction  [tex]MgCO_{3}[/tex] white precipitate.

Learn about replacement reaction.

https://brainly.com/question/17970302

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is a planar carbon "backbone" possible for propane? Explain.


Is a planar carbon "backbone" possible for cyclopropane? Explain.

Answers

Answer:

Cyclopropane has a planar carbon back bone while propane does not

Explanation:

We have to recognize that in straight chain saturated organic compounds, carbon atoms have a tetrahedral geometry. Each carbon atom is bonded to four other atoms.

However, carbon atoms in cyclic compounds are also sp3 hybridized with each carbon bonded to only four other atoms but the ring system is highly strained.

Cyclopropane is a necessarily planar molecule with a bond angle that is far less than the expected tetrahedral bond angle due to strain in the molecule. Hence, the carbon atoms may have have a "planar backbone".

Can I please Get Help with this question ‍♂️ ?

Answers

Answer:

Mg(NO4)2 is 180.3 g/mol

Explanation:

First find the substance formula.

Magnesium Nitrate.

Magnesium is a +2 charge.

Nitrate is a -1 charge.

So to balance the chemical formula,

We need 1 magnesium atom for every nitrate atom.

2(1) + 1(-2) = 0

So the substance formula is Mg(NO4)2.

Now find the molar mass of Mg(NO4)2.

Mg = 24.3 amu

N = 14.0 amu

O = 16.0 amu

They are three nitrogen and twelve oxygen atoms.

So you do this: 24.3 + 14.0(2) + 16.0(8) = 180.3 g/mol

So the molar is mass is 180.3 g/mol.

The final answer is Mg(NO4)2 is 180.3 g/mol

Hope it helped!

how an object accelerates when it changes direction?​

Answers

Answer:

An object which experiences either a change in the magnitude or the direction of the velocity vector can be said to be accelerating. This explains why an object moving in a circle at constant speed can be said to accelerate - the direction of the velocity changes.

Explanation:

Answer:

An object which experiences either a change in the magnitude.

Explanation:

the direction of the velocity changes.

Hope that helps :)

Have a good day

What is the ratio of hydrogen atoms to oxygen atoms in a water molecule?​

Answers

2:1
The ratio of hydrogen atoms to oxygen atoms used to make water molecules is always 2:1, no matter how many water molecules are being made.

Can you please help me

Answers

Shure what you need help with

Identify the good electrical conductor. Carbon Copper Nitrogen Sulfur​

Answers

Copper is a good electrical conductor because it's metal. Carbon, Nitrogen, and Sulfur aren't metals, so they are poor conductors of electricity.

All moving objects have kinetic energy. True False

Answers

Answer:

true

.......................

Answer: You're Welcome!

Oh, It's False - Kinetic energy depends upon the speed of the object; potential energy depends upon the position of the object.

Imagine an alternate universe where the value of the Planck constant is 6.6207 x 10^-36 J*s.
In that universe, which of the following objects would require quantum mechanics to describe, that is, would show both particle and wave properties? Which objects would act like everyday objects, and be adequately described by classical mechanics?
3.A turtle with a mass of 450. g, 29. cm long, moving at 2.7 cm/s.
quantum or classical?

Answers

Answer:

See explanation

Explanation:

According to Louis de Broglie, particles could exhibit wavelike properties and have an associated wavelength.

Now for a turtle with a mass of 450. g, 29. cm long, moving at 2.7 cm/s, recall that we can only describe a by quantum mechanics when the body is very small and its associated wavelength is large.

This object has a large mass, hence it is discussed by classical rather than quantum mechanics

The specific heat capacity of methane gas is 2.20 J/g-K. How many joules of heat are needed to raise the
temperature of 5.00 g of methane from LaTeX: 36.0^\circ C36.0 ∘ C to LaTeX: 75.0^\circ C75.0 ∘ C?

Answers

Heat needed to raise the temperature : Q = 3432 J

Further explanation

Given

c = The specific heat capacity = 2.20 J/g-K

m = mass = 5 g

Δt = Temperature difference = 75-36 =39 °C=312 K

Required

Heat needed

Solution

Heat can be formulated

Q = m.c.Δt

Input the values :

Q = 5 g x 2.2 J/g-K x 312 K

Q = 3432 J

Analysis of an unknown substance indicates that it is a brittle solid. It acts as an electrical insulator (does not conduct) as a solid but conducts electricity when melted. Which of the following substances would have these characteristics?

a. HCl
b. Al
c. KBr
d. SiF4
e. I2

Answers

Answer:

KBr

Explanation:

A metal has a very high melting point. Aluminum is a metal.

Ionic solids do not conduct electricity in the solid state. However, when the solids are melted, they conduct electricity.

KBr is an ionic substance, it can only conduct electricity in molten form because the ions are locked up in the solid.

how long does crushed candy dissolve in a warm water​

Answers

Answer:

Because of reaction between solid and liquid

Answer:

shouldn't take that long i would say from 30 to 40 minutes bc its melting in warm water its turning from a solid to a liquid.

Explanation:

.What type of energy includes both kinetic and potential energy?

Answers

Answer:

mechanical energy

Explanation:

How many sulfur atoms are in 5 SF6 ?

Answers

Answer:

The molecular formula for sulfur hexafluoride is SF6 S F 6 , in which there is 1 sulfur atom and 6 fluorine atoms.

Explanation:

hope this helps

what is the difference between the number of electrons in an atom of selenium, Se and the number of electrons in an atom of aluminum, Al?

Answers

Answer:

Well, electrons can be converted into a atomic number so if SE atomic number is 34 that means it has 34 electrons. AI has a atomic number of 13 meaning it has 13 electrons. So the difference is that SE has more electrons then AI.

Which of the following best explains why Reggie's books fell?

Answers

Explanation:

When Reggie's car turned eastward, his books continued heading northward, causing them to slide off the top of his car and fall.

were is the opshons.

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