What happened if we add normal saline instead of PBS during beta amylase extraction?​

Answers

Answer 1

Answer:

hi

Explanation:

Answer 2

Saline is the solution of water and NaCl does not provide the appropriate pH, so enzyme activity is lost, we cannot add saline instead of PBS during beta amylase extraction.

How saline water affects beta amylase extraction?

PBS (phosphate buffer solution) is a type of buffer that is suited for beta-amylase activity, which implies that when beta-amylase is extracted using buffer, the enzyme's chemical activity is kept.

When we utilize saline, which is a solution of water and NaCl, the pH of the solution is not acceptable, and the enzymes' chemical activity is destroyed.

As a result, for enzyme/protein extraction, we always employ a buffer solution, which maintains the ph for the proper functioning of an enzyme.

Therefore due to pH conditions enzyme lost activity, and normal saline water can not be used instead of PBS for extraction of beta-amylase.

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Related Questions

The _______________ has a negative charge. The ______________ is smaller than the other particles and can be found outside of the ___________________, which is another name for the center of the atom. In the center, there are ________________ and ___________________. The __________________ have no charge but weigh a lot compared to the ________________. The proton has a __________________ charge. In order to get the atomic mass, add the number of ________________ and _________________ together

Answers

Answer:

The electron has a negative charge. The electron is smaller than the other particles and can be found outside of the nucleus, which is another name for the center of the atom. In the center, there are protons and neutrons. The neutron have no charge but weigh a lot compared to the electron. The proton has a positive charge. In order to get the atomic mass, add the number of protons and neutrons together.

Explanation:

Answer:

electron

?

nucleus

protons

neutrons

neutrons

electrons

positive

protons

protons

neutrons

(because electrons weigh so little it dosnt make a difference)

Black mice and tan mice are native species that are observed in a particular region. Human habitation in the region caused an invasive mouse species to enter the region. These mice are brown and share the same food requirements as black mice. Tan mice have different food requirements. How will this change affect the food web in the region? A. The availability of food resources for black mice and brown mice will decrease. B. The population of tan mice will decrease. C. The black mice and tan mice will compete for food resources. D. The tan mice and brown mice will compete for food resources.

Answers

Answer:

A

Explanation:

The correct answer would be that the availability of food resources for black mice and brown mice will decrease.

Since the food requirements of the black mice are the same as that of the invasive brown mice, the available food supply that used to be only for the black mice would now be shared by the two strains of mice. Hence, the available food for the two groups of mice will naturally decrease.

There is no sufficient information to conclude that the population of tan mice will decrease, hence, option B is incorrect.

The black mice and tan mice have different food requirements going by the information available in the illustration, hence, both cannot compete for food resources. Option C is, therefore, incorrect. In the same vein, option D is incorrect because the tan mice have different food requirements from the brown mice.

The only correct option is A.

Which of the following foods contain MOSTLY lipids? (Check all that apply)

Bread
Broccoli
Soda
Pasta
Olive oil
Fish
Butter
Nuts
Steak

Answers

Answer:

Olive oil.

Explanation:

Edible oils are lipids. These include vegetable oils such as:

canola cottonseed grape seed corn olive peanut and many others

Why are plants intelligent?

Answers

Answer:hope tht helped

Explanation:

What is formed as a result of mitosis?
A.four genetically different cells.

B.four genetically identical cells

C.two genetically different cells

D.two genetically identical cells

Answers

Answer:

D. two genetically identical cells.

Explanation:

What happens in an endothermic reaction?

Answers

Answer:

In endothermic reactions, more energy is absorbed when the bonds in the reactants are broken than is released when new bonds are formed in the products. Endothermic reactions are accompanied by a decrease in temperature of the reaction mixture.

If anyone knows this please let me know and explain??

Answers

Answer:

I am pretty sure the answer is Melting

Explanation:

C) Evaporating

The molecules in the first state are liquid (you can tell how they aren’t in a fixed structure) break apart randomly and evaporate into a gas

Suppose you eat a serving of green beans. What reactions must occur for the amino acid monomers in the protein of the beans to be converted to proteins in your body?

Answers

It’s ribosome it’s what makes the whole system.

Products of the breakdown of an original pesticide or herbicide are called __________.
A)
silt
B)
degradates
C)
soil peds
D)
cations
E)
aggregates

Answers

Answer:

B

Explanation:

degradates

An amoeba ingests large food particles by​

Answers

From a method called phagocytosis.

What wave travels along the surface of a medium?

Answers

ocean waves. Reason- A surface wave is a wave that travels along the surface of a medium. Ocean waves are the best-known examples of surface waves. In a surface wave, particles of the medium move up and down as well as back and forth in an overall circular motion.

hich two phrases in this passage point to the meaning of the word stealthily?

The question in her mind, and one not easily solved, now was, 'How can I get away?' So, as was her usual custom, she 'told God she was afraid to go in the night, and in the day every body would see her.' At length, the thought came to her that she could leave just before the day dawned, and get out of the neighborhood where she was known before the people were much astir. 'Yes,' she said, fervently, 'that's a good thought! Thank you, God, for that thought!' So, receiving it as coming direct from God, she acted upon it, and one fine morning, a little before day-break, she might have been seen stepping stealthily away from the rear of Master Dupont's house."

direct from God
get away
bulk and weight
usual custom
before day-break

Answers

Answer:

B and E

Explanation:

Because thats what stealthy means

The meaning of the word stealthily is get away and before day-break.

What is stealthily?

You can enter a building discreetly at times, such as when users creep about quietly in the darkness to prevent being seen.

What is get away?

Get away means flee from someone or somewhere;

Hence, The meaning of the word stealthily is get away and before day-break.

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Which of the following molecules are classified as carbohydrates?

Answers

Ones that have c,h,o in a 1:2:1 ratio

The molecules classified as carbohydrates are oligosaccharides, monosaccharides, disaccharides.

What are the carbohydrates?

Carbs, or carbs, are sugar particles. Alongside proteins and fats, starches are one of three fundamental supplements tracked down in food varieties and beverages.

Your body separates starches into glucose. Glucose, or glucose, is the principal wellspring of energy for your body's phones, tissues, and organs. Glucose can be utilized right away or put away in the liver and muscles for sometime in the future.

There is nobody size-fits-all measure of starches that individuals ought to eat. This sum can differ, contingent upon variables like your age, sex, wellbeing, and whether you are attempting to lose or put on weight. Overall, individuals ought to get 45 to 65% of their calories from carbs consistently.

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1) In yeast signal transduction, a yeast cell releases a mating factor which
A) acts back on the same cell that secreted the mating factor, changing its development
B) passes through the membranes of neighboring cells, binds to DNA, and initiates transcription
C) binds to receptors on the membranes of other types of yeast cells
D) diffuses through the membranes of distant cells, causing them to produce factors that initiate
long-distance migrations

Answers

I think it is A it acts back on the same cell

In yeast signal transduction, a yeast cell releases a mating factor that diffuses through the membranes of distant cells, causing them to produce factors that initiate long-distance migrations. Thus, the correct option for this question is D.

What is the mechanism of reproduction in yeast?

The mechanism of reproduction is yeast is processed through the process of budding which is a type of asexual reproduction. In this mechanism, A bud is a tiny protrusion from the parent cell that pinches off and produces a new individual when it reaches maturity.

Yeast is a single-celled organism that does not have complexity in its process and functions. So, this organism performs its mechanism of reproduction and other functions through the process of diffusion.

Therefore, in yeast signal transduction, a yeast cell releases a mating factor that diffuses through the membranes of distant cells, causing them to produce factors that initiate long-distance migrations. Thus, the correct option for this question is D.

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Perception is:
O A. creating images through your experiences.
O B. picking up information and relaying it to the brain.
O C. the ability to work top down or bottom up.
O D. organizing and interpreting sensory input.

Answers

B) the ability to work top down bottom up



B it the answer Fasho

If a strand of DNA has 17% T,what percent will
be G? C? A?

Answers

Answer:

If a DNA strand has 17% Thymine (T) the percentage of the other nitrogenous bases will be 33% of Guanine (G), 33% of Cytosine (C) and 17% of Adenine (A), according to Chargaff's law.

Explanation:

The percentage of nitrogenous bass in a DNA strand can be established by knowing the percentage of one of the bases present in the molecule, in this case knowing that Thymine corresponds to 17% of bases.

DNA has the nitrogenous bases adenine (A), guanine (G), thymine (T) and cytosine (C).  The bases of one chain are matched with the bases of another, according to the complementarity of the nitrogenous bases, where:

Adenine is complemented with thymine A=TGuanine is complemented by cytosine G≡C

According to this, DNA molecule there is as much T as A, and an equal amount of G and C, and the percentages of nitrogenous bases can be calculated according to Chargaff's rule.

Chargaff deduced that in a DNA molecule the ratio of purine:pyrimidine is 1:1, so there must be the same amount of thymine as adenine and a similar amount of guanine for the cytosine, according to the complementarity of the bases.

Calculating the percentages, according to the law of the base pair, if in a DNA chain there is 17% of T, in the molecule there is:

A =          17 %C =          33%T =           17%G =          33%Total ..... 100%

Which of the following helps to keep the biological membranes in a fluid state?
A.Waxes
B. Cholesterol
C. Phospholipids
D. Water​

Answers

Answer:

C. Phospholipids

Explanation:

So sorry if I am wrong, but I hope this helps!

The answer is Phospholipids, I can’t explain but the phospholipids are placed in water, so they keep the biological membrane in the fluid state.

Light energy is converted to chemical energy which is what

Answers

Photosynthesis. “Photosynthesis is the process in which light energy is converted to chemical energy.”

What gas in the atmosphere helps to protect us from that radiation

Answers

Answer:

ozone

Explanation:

All living organisms on the earth are protected from the harmful radiation coming from the sun by the ozone gas present in the stratosphere. Ozone gas is formed by the action of sunlight only oxygen of the air in the atmosphere.

Answer:

ozone gas

Explanation:

2. The structural and functional unit of life is (a) a cell, (b) an organ, (c) the organism, (d) a
molecule.

Answers

Answer:

A. A cell

Explanation:

Cells are the building blocks of life.

They are the biological units of organisms and are the basic structural and functional units of life.

Cells are the smallest units of life.

In the United States, it is possible for discoverers of gene sequences in organisms to patent those sequences. This gives the discoverer property rights for the sequence. Many scientists object to this practice. Which is the strongest scientific argument against allowing a gene sequence to be patented?
Group of answer choices

Patenting sequences prevents conservationists rom understanding the ecological needs of organisms from which the patented sequences come.

Patenting sequences prevents he sequences from being expressed in nature.

Patenting sequences prevents researchers from studying and creating innovations from the patented sequences.

Patenting sequences prevents the sequences from being used in genetically modified organisms.

Answers

Answer:

Explanation:

There is little evidence that early fears about gene patenting placing substantial ... owner and do not protect against independent invention), patents require disclosure that ... gene knock-out methods and even for individual gene sequences. ... Human gene patents result from the cloning and description of the sequence of a ...

so option c maybe

True or false (look at the pic below for the question)

Answers

Answer: True

Explantion:                                                                

Answer:

I am going to say true

Explanation:

Plants often store food in their stems. Roots - The roots of a plant grow underground. Roots help to keep the plant from falling over and gather water and minerals from the soil. Some plants store food in their roots.

Please correct me if I am wrong :)

I’m thinking both what do you all think??

Answers

Answer:

I actually think a chemical change

Explanation:

The chemical composition should not change in a physical change so therefore I think it’s only a chemical change

In guinea pigs, smooth coat (S) is dominant over rough coat (s), and black coat (B) is dominant over white coat (b). In the cross SsBb × SsBb, how many of the offspring will have a smooth black coat, on average?
a. 1⁄4
b. about 9⁄16
c. 1⁄16
d. 6⁄16
e. 2⁄6

Answers

Answer:

The correct answer is - b. about 9⁄16

Explanation:

I the given question is given:

Smooth coat (S) is dominant over rough coat (s), and black coat (B) is dominant over white coat (b)

Then cross betwen SsBb × SsBb

gametes:

SB, Sb, sB, and sb for each parent:

        SB       Sb      sB      sb

SB   SSBB  SSBb   SsBB  SsBb

Sb   SSBb  SSbb   SsBb  Ssbb

sB    SsBB  SsBb    SsBB  ssBb

sb    SsBb   Ssbb    ssBb   ssbb

every offspring with SB in the combination will express a smooth black coat that is 9 according to the Punnett out of 16 offspring.

Mice and rabbits eat plants. They are known as herbivores.
Mice, and rabbits are also called ____________.
A. consumers
B. decomposers
C. producers
D. predators

Answers

Answer:

A. Consumers

Explanation:

They get from other organisms. They don’t give nor decompose. Predators are carnivores

Fifty years ago, bed bugs were nearly eliminated in the United States by using of pesticides like DDT. Today bed bugs are back in beds and theaters. DDT is no longer used due to environmental issues. Pyrethrums are currently the top choice for bed bug infestations. Pyrethrums are especially useful to us because they generally have a stronger effect on bugs than on humans and animals.

Some bed bugs had mutations in their genetic code which allowed them to survive the chemical pesticide and they produced chemically resistant offspring. How can this be explained?

Question 5 options:

bed bugs chose other bugs to partner with who had the mutation


mutations involved in the genetic variation of the bugs were chosen by them


bed bugs chose other bugs to partner with who had no mutations


mutations involved in the genetic variation of the bugs are random

Answers

d

Explanation:

i looked up and it said mutation random

Which of the following foods contain MOSTLY proteins? (Check all that apply)

Bread
Broccoli
Soda
Pasta
Olive oil
Fish
Butter
Nuts
Steak

Answers

Answer:

ok here goes

Explanation:

mostly all proteins contain fats and oil so I'll take Butter,nuts,steak,olive oil mostly as my answer according to me fish kinda has adequate proteins to carbohydrates

(hope i was helpful, thanks for the chance)

Most of the milk products and by-products consist of proteins.  Items like Bread, Pasta Olive oil , Fish , Butter , Nuts . Even steak also consists of protein in large qualities.

Protein is a building block of the body and mostly found in animal and plant bodies. It is a important part of the healthy diet and is good source of nutrition.

Most of the oils, butter, nuts and cheese consists of fats that consist of enzymes and are used as an energy source of tonic.  Hence the option olive oil, nuts, butter, fish and steak consists of high amount of protein.

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Tissues are groups of similar _____ performing the same function.

Answers

Answer: A tissue is a group of similar cells together. An organ is groups of two or more tissue. An organ system is a group of organs.

Explanation:

During photosynthesis energy from the sun is converted into

Answers

The answer is Nutrients the plant needs
During photosynthesis energy from the sun is converted into glucose, solar energy is harvested as a chemical energy in a process that converts water and carbon dioxide to glucose

Cellular Respiration is mainly about the release of what?

Answers

Answer:

Carbon Dioxide

Explanation:

Water is also released but its mainly carbon dioxide

Answer:

Cellular respiration includes the releasing of ATP.

Explanation:

Cellular respiration take place in the cells and produces ATP.

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A___ Relationship would include your co-workers and friends. You have completed 35% of your workout. Your workout is 35 minutes long. How much time do you have left? fine the slope of the table below HELLPPP Fill in the blank____ crystallizes from fluid lava and points to the north magnetic pole is the lava cools What is the result of subtracting the second equation from the first?x3y=68xy=6 elaborate on why colonist dressed up as native Americans during the Boston tea party. QUICK WILL GIVE BRAINLIEST. what is equivalent to the expression 2/5 divided by 4 Stanzas containing five lines are called:A. quintainsB. sestetsC. tercetsD. couplets it is a principle that incorporates time to rest into the fitness program The thirteen english colonies are on which coast of the north america?A. The eastern coast B. The southern coastC. The western coast. HELLLP I WILL GIVEVU THAT CROWWWWWWNDinner threw me deeper into despair. My relatives licked the ends of their chopsticks and reached across the table, dipping them into the dozen or so plates of food. Robert and their family waited patiently for platters to be passed to them. My relatives murmured with pleasure when my mother brought out the whole steamed fish. Robert grimaced. Then my father poked his chopsticks just below the fish eye and plucked out the soft meat. "Amy, your favorite," he said, offering me the tender fish cheek. I wanted to disappear.Which details could illustrate a paragraph on imagery? Select all that apply.Dinner threw Amy deeper into despair.Amys relatives licked the ends of their chopsticksRobert and their family waited to be servedAmys father hands her a tender fish cheek. Amy wants to disappear. HELP ASAP6+ x - y + 10= Article III of the U.S constitution gives the supreme court justices the power toA. serve for the rest of their livesB. pass laws all states must obeyC. punish judges for using judicial activism D. create new federal district courts For the function w, w(3) = -2 and w(-2) = 8. If y = w(x), what is the value of y whenx = -2? what are pros and cons plz explain :D i don't understand :/ 18 x 5 Use mental math to solve. Find as many strategies as you can to solve. Record and describe each strategy. Find x and y so that the quadrilateral is a parallelogram. The Census Bureau says that the 10 most common surnames in the United States are, in order, Smith, Johnson, Williams, Brown, Jones, Miller, Davis, Garcia, Rodriguez, and Wilson. These names account for 9.6% of all U.S. residents. Suppose you look at the authors of the textbooks for your current courses and find that there are 8 authors in total. What is the probability that none of the 8 surnames of these authors were among the 10 most common Which of the following was areason for many to settle out in the West under the Homestead Act?A. the frequent droughts in the New EnglandstatesB. the land was yours at no cost after five yearsC. the invention of the automobileD. the demand for wheat and corn to beproduced