You plan to clone a guide RNA sequence into the pML104 plasmid because you want to engineer the construct in bacteria and later express it in yeast. Unfortunately, your lab does not have any available pML104. Your friend in the lab next door lets you borrow a related plasmid pML107, and you quickly start your experiment. Will it work? Briefly explain.
Answer:
Yes, the experiment will work.
Explanation:
The reason behind this is that plasmids are well known to be DNA vector carriers. These plasmids have the ability to bring about changes in DNA fragments into various microorganisms and reveal them. The two plasmids compose of the same genomic marker such as the antibiotic resistance gene found in URA3. However, plasmids are most commonly found in bacteria, but also in eukaryotic cells. Oftentimes, the gene composition in the plasmids enhances the bacteria with a vantage edge such as antibiotic resistance.
Hence, it is obvious that in both, the molecular regulation in terms of expression of genes is alike in properties. Similarly, the numbers of copies and molecular weight are also the same.
We can thereby conclude that, provided that there is a proper restriction site, another host that is up to standard for plasmid can be replaced for each other.
True or false:Winds are named by the direction they come from.

ANSWER:
TRUE.
Explanation: Because winds are named from the direction in which they originate, these winds are called prevailing westerlies.
Which of the following is an example of two organisms competing for an abiotic factor?
A. Two different species of insects
competing for leaves from trees
B. Two different species of
mammals competing for small
rodents to eat
C. Two different species of birds
competing for berries to eat
D. Two different species of
mammals competing for the
same water source
Two different species of mammals competing for the same water source is an example of two organisms competing for an abiotic factor.
What is abiotic factor?
Abiotic factors refers to non-living physical parts of the environment that affect living organisms as well as the functioning of ecosystems. In the above example, water source is a non-living part of an ecosystem which is required by both organisms.
This leads to competition of two mammals so we can conclude that two different species of mammals competing for the same water source is an example of two organisms competing for an abiotic factor.
Learn more about mammals here: https://brainly.com/question/935140
Human growth hormone (HGH) is necessary for growth during human adolescence. Pituitary dwarfism is a condition that results from the inability of a person to produce HGH. Luckily, the human gene for HGH can be inserted into E. coli bacteria, which are able to make our HGH. The resulting HGH is used by people who are unable to make their own. What property about hereditary information makes this possible
Answer:
The correct answer is - DNA is a genetic language that is common and uses by every organism including humans and bacteria.
Explanation:
DNA is the unit of heredity and present in almost every living organism, it carries the genetic information and transfer from generation to generations. In this process of transfer of the genetic information, there are two processes transcription and translation helps to produce protein.
The ability to produce proteins with the help of DNA for the particular function as said in question which is HGH production. Correcting the abnormality of not able to produce HGH, to produce the normal protein with the help of putting human genes into Bacteria as DNA is universal genetic language.
Which behavior is a response to an external stimulus?
A. An elephant drinks water to quench its thirst.
B. A dog vomits during an illness to rid itself of pathogens.
C. A child gets a fever to fight off the flu.
D. A turtle suns itself on a rock during a cool day.
Answer:
The answer is in picture below
Explanation:
Hope this helps <3
True or False: If you break / cut a magnet in half, two magnets (each with a North and South pole) will be created.
Answer:
true
Explanation:
the gratational pole attrachts it
The continental crust and oceanic crust together make up the ________
A. lithosphere.
B. asthenosphere.
C. lower mantle.
D. upper mantle.
Use the following table to answer the question:
Element
Mass Number
Atomic Number
Aluminum
27
13
Nitrogen
14
7
Helium
4
2
Fluorine
19
9
please help
Which element has the least neutrons in the nucleus?
Aluminum
Nitrogen
Helium
Fluorine
Answer:heh
Explanation:
Aluminium , mass no = no of proton + neutron = 27
atomic no = no of proton = 13
no of neutron = 27 - 13 = 14
Nitrogen , mass no = no of proton + neutron = 14
atomic no = no of proton = 7
no of neutron = 14 - 7 = 7
Helium , mass no = no of proton + neutron = 4
atomic no = no of proton = 2
no of neutron = 4 - 2= 2
fluorine , mass no = no of proton + neutron = 19
atomic no = no of proton = 9
no of neutron = 19-9 = 10
Answer:
helium
Explanation:
i took the test and got it right... it also has the smallest number
The color of a light wave is determined by its wavelength. On the COLOR tab, slowly drag the Light wavelength slider back and forth and observe the effect on oxygen production. How does the color of light affect the rate of photosynthesis
Answer:
The color of light determines how effective chlorophyll pigment will be at absorbing light.
Explanation:
Photosynthesis is a metabolic process that requires light, which is an electromagnetic radiation. Light on the electromagnetic spectrum is determined by wavelength. Each region/color of light occurs at a specific wavelength.
Hence, chlorophyll pigment, which is the major photosynthetic pigment effectively absorbs blue and red light in the visible light region of the electromagnetic spectrum. Hence, the rate of photosynthesis will be affected negatively if the color of light is not the one stated above.
Plants will grow more when the day is longer
is this supposed to be a true and false question... or is this a statement you were making...
what is the general trend in the elk and wolf populations between 1990 and 2000?
Answer: Decrease
Explanation:
which of the following is necessary for cellular respiration and photosynthesis to occur
Answer:
Sunlight!
Explanation:
What are the functions of a petal
What is the correct "height" or "size" for a piece of oils rigs to become a candidate for "rigs to reef." Remember, oil rigs can be cut into different sizes or parts.
Answer and Explanation:
An oil rig is a type of piston ring which is situated in the ring grooves. The oil ring is commonly used on small engines to wipe excess oil from the wall of the cylinder during the piston's movement. Through the opening of rings, excess oil returned to the oil reservoir. These rings are not used on the two-stroke cycle engines. These rings are one-piece or two-piece. An oil ring seals the combustion chamber through inherent pressure and controlling the consumption of oil. The inherent pressure of the oil ring is very high on the piston. These rings use an expander, high-pressure unit and inherent pressure. The oil rings are also used to spread the oil around the liner surface. These rings can be cut into various sizes. An oil ring has an axial.
the diagram shows a bacterium. Which labels best complete the diagram
Answer:
X - Flagellum, Y - Bacterial DNA, Z - Cytoplasm
Explanation:
A bacterium is a living thing that are majorly known for causing diseases but are important for human life.
A bacterium is also consist of several organelles such as cell wall, cytoplasm, flagellum, bacterial DNA, cytoplasm, ribosomes and others.
The diagram shows three main organelles that are X - Flagellum, Y - Bacterial DNA, Z - Cytoplasm. Flagella functions for the locomotion of the bacteria, bacterial DNA is a genetic material of the bacteria, and cytoplasm is a gel-like matrix composed of enzymes, water, nutrients and other waste material.
Hence, the correct answer is "X - Flagellum, Y - Bacterial DNA, Z - Cytoplasm".
What is the definition of taxonomy?
A. A science, law, or principle of classification that helps build taxonomic trees that map the development and evolution of a species
B. A science, law, or principle of classification that allows biologists to map the development of species that are still evolving
C. A science, law, or principle of classification that helps classify information on a species based purely on their Genus and Species
Answer:
Option (C) is the correct answer.
Explanation:
Taxonomy: It is a process of classifying, organizing, and naming things such as plants, animals, microorganisms, and other living things.
Carolus Linnaeus a father of taxonomy,
Taxonomy utilizes hierarchical arrangement.
There are eight taxonomic categories:
Domain
Kingdom.
Phylum.
class.
Order.
family.
genus
Species.
Why are models useful in Earth science?
Answer:
because it represents what happens
Explanation:
What was happening in history when this mutation took off in parts of Europe and Africa?
PLEASE SOMEONE HELP ME I’M REALLY STRUGGLING
Answer:seventy-seven thousand years ago, a craftsman sat in a cave in a limestone cliff overlooking the rocky coast of what is now the Indian Ocean. It was a beautiful spot, a workshop with a glorious natural picture window, cooled by a sea breeze in summer, warmed by a small fire in winter. The sandy cliff top above was covered with a white-flowering shrub that one distant day would be known as blombos and give this place the name Blombos Cave.
Explanation:
How does energy from the Sun travel to Earth?
through radiation
through convection
through conduction
through the movement of particles
Answer:
A
Explanation:
its right on the enviromental systems cumlative exam on edg 2021
The energy from the Sun travel to Earth through radiation.
Heat transfer by radiation
Radiation is a method of heat transfer that does not require a material medium for the heat transfer process.
Energy transfer from the sunThe energy transfer from the sun reaches the earth through radiation process since the the energy travels in wave form.
Thus, the energy from the Sun travel to Earth through radiation.
Learn more about radiation here: https://brainly.com/question/24469662
During Prophase I replicated homologous chromosomes pair up and undergo synapsis. What testable question is generated regarding synapsis and genetic variability by Figure 1?
A. Is the distance between two gene loci related to crossover rate?
B. Does crossing over occur more often in some chromosomes than in others?
C. Is crossing over inhibited by methylation? D. Is crossing over promoted by methylation?
Answer: A
Explanation: I saw it on quizlet
1. Which of the following is NOT a characteristic of life.
A. Being Multicellular
B. Responsiveness to the Environment
C. Growth and Change
D. Maintain Homeostasis
Answer:
it may be a or d but b and c are a characteristic in life
2. Name a microbe that lacks a cell wall and would find hypotonic environments detrimental.
Answer:
I so I am also a bit confused, but I hope this could answer your question!
Explanation:
The bacteria possess a cell wall composed of peptidoglycan molecule formed by the amino acids called murine. The bacterial cell wall helps in the survival of the bacteria in some extreme condition like when placed in a hypotonic solution.
The hypotonic solution is formed when it has low solute concentration and high water amount when compared. When the bacterial cell will be placed in hypotonic solution then the water will move inside the cell down the concentration gradient as a result of which the bacterial cell will swell but will not burst.
This happens as the cell wall contains expansin protein which allows the cell wall to expand and provides rigidity to the cell.
Answer:
1. Photoautotroph
2.Protozoa
3. Passive transport uses no energy and molecules move from high to low concentrations. Active transport uses energy to move from low to high concentrations.
4. Synergism
Explanation:
Good luck on your assignment
Why is understanding the niche of tree species important in forest management?
A)
It helps determine what kind of fertilizer is needed.
B)
It helps determine where the trees can be best planted or cultivated.
C)
It helps determine the type of organisms that use it as a habitat.
D)
It helps determine what kind of weather will affect it.
Answer:
B
Explanation:
Edge 2020-2021
1. Robin carries the trash from the house to the garbage can outside. Which statement best describes the science of what he did?
He would use less energy if he were to move the garbage faster
He used energy to carry the garbage.
He did not have to work because garbage is light.
He caused the garbage to have potential energy when it moved.
Answer:
He used energy to carry the garbage.
Explanation:
Energy is the ability to do work. When work is done, energy is expended during the process. Work is done if the force applied on an object moves that object through a distance.
In the case where Robin carries the trash from the house to the garbage can outside, work is done because, the force applied by Robin on the trash effectively helps to move the trash from inside the house to the garbage can outside the house. During the process of moving the trash, Robin expends or uses energy to do the work.
From the given options:
He would use less energy if he were to move the garbage faster is wrong because an increase in speed results in an increase in energy
He used energy to carry the garbage is correct because energy is the ability to do work
He did not have to work because garbage is light is wrong because as long as an object is moved a distance, work is done irrespective of the mass of the object.
He caused the garbage to have potential energy when it moved can only be true if he had raised the garbage from the floor to a height above the ground. However, he only moved it fro inside to outside the house.
What is the role of helicase in DNA replication?
Answer:
The helicase is essential because they separate the double-stranded DNA into single strands so that it could be copied.
Hoped this helped!
Sickle cell anemia and albinism are both recessive traits in humans. Imagine that a couple, already pregnant with fraternal twins, has just learned that they (the couple) are both heterozygous for both of these traits. As the couple's genetic counselor, the couple asks you what is the probability of having both of the twins be albino and have sickle-cell anemia.
Answer:
81/256
Explanation:
By doing a test cross of albinism and sickle cell anemia you'll see that the probability that one child is unaffected by either conditions will be 9/16. Both children would be 9/16 * 9/16.
Since fraternal twins are developed from two separate fertilization events the probability chance will be independent.
The incidence of V. vulnificus infections is increasing, especially in northern parts of the United States, compared to 20 years ago. Can you think of any reason why this might be happening?
Answer:
The effects of climate change may be associated with the increased incidence of V. vulnificus infection
Explanation:
Vibrio vulnificus is a harmful bacterium that causes a serious disease due to eating contaminated seafood, which is the main cause of seafood-related deaths in the USA. V. vulnificus is naturally present in the flora of coastal waters around the world and this bacterium has been isolated from a variety of seafood (e.g.., fish, oysters, shrimp, etc). Epidemiologic studies have shown that the incidence of V. vulnificus infection has increased dramatically since 1996. During this same period, climate change has been responsible for the increase in salinity and temperature of the coastal waters (i.e., the natural habitat of this bacterium). In consequence, it is reasonable to suppose that the change in climatic conditions may be associated with the increased incidence of V. vulnificus infection.
A disease infects an organism, causing the organism's cells to have less chloroplasts. How will the organism be impacted by having less chloroplasts?
The organism will have less chlorophy reducing its ability to collect energy
The organism will have less cellulose, reducing its ability to remove waste
The organism will absorb less oxygen, reducing its ability to produce sugar
The organism will absorb less water, reducing its ability to create DNA
Answer:
the organism will have less chlorophyll
Explanation:
chloroplasts contain chlorophyll
Chlorophyll is a green pigment, without it a plant is discolored meaning it lacks of chlorophyll. Less photosynthesis would occur, and less glucose is made as a result. There is less energy released for growth as glucose is needed for respiration.
3. A population of 70 jaguars lives in a rainforest measuring 350 square miles. What is the population
density of the jaguars? (Hint: You cannot have a fraction of a jaguar)
Answer:
5 jaguars per square mile.
Explanation:
If you do 350 ÷ 70 it comes to 5, so that means it's 5 jaguars per square mile. Have a fun day. :)
The population density of jaguars in the rainforest is 0.2 jaguars per square mile.
To calculate the population density, we divide the total number of individuals (70 jaguars) by the area of the habitat (350 square miles).
Population density = Total number of individuals / Area
Population density = 70 jaguars / 350 square miles
Simplifying the calculation, we find that 70 divided by 350 equals 0.2. Therefore, the population density of jaguars in the rainforest is 0.2 jaguars per square mile.
Population density is a measure of how concentrated a population is within a given area. In this case, it tells us how many jaguars are found on average in each square mile of the rainforest. A population density of 0.2 jaguars per square mile means that, on average, there are 0.2 jaguars in every square mile of the rainforest.
It's important to note that population density can vary across different habitats and species. Factors such as food availability, territory size, and reproductive behavior can influence the distribution and concentration of individuals within a given area. Understanding population density helps researchers assess the health of a population, monitor changes over time, and make informed conservation and management decisions.
To learn more about population density, here
https://brainly.com/question/16894337
#SPJ2
What will we do with waste materials? Waste water, garbage, sewage, industrial pollutants--the amount of waste produced grows
each year. The increasing amount of waste materials also requires a growing need for professionals in the field of
A)
world politics.
B)
energy conservation
C)
environmental engineering
D)
marketing and cost analysis.
Answer: environmental engineering
Explanation:
