When performing the streak for isolation technique, your result is unexpected: There is only growth obtained in quadrant 1. The growth observed in quadrant 1 is as expected, heavy with no isolated colonies. However, no growth is visible in any other quadrants.
Which of the following errors could explain this unexpected result?
A) The loop did not cross into quadrant 1 when streaking quadrant 2
B) You forgot to flame the loop in between quadrants
C) All of the errors listed here could be responsible for this unexpected result
D) Not enough bacteria was obtained from the starting culture when streaking quadrant 1

Answers

Answer 1

Answer:

A) The loop did not cross into quadrant 1 when streaking quadrant 2

Explanation:

The streak for isolation technique is a method used to get isolated bacterial colonies. This technique consists of, in a petri dish with culture medium, where, with the help of an inoculation loop, the bacteria are scratched on one side of the dish. This side is called the first quadrant. This inoculation loop must then cross with the first quadrant and be dragged to the second quadrant, where new lines must be made. This allows a part of the bacteria from the first quadrant to be carried over to the second. The inoculation loop must cross the second quadrant again to inoculate the third quadrant, and so on.

In this case, we can see that for bacterial colonies to grow in all quadrants, it is essential that the loop crosses in the first quadrant to cross out the second quadrant. If not, only the first quadrant will have bacterial colonies.

Answer 2

Answer:

A) The loop did not cross into quadrant 1 when streaking quadrant 2

Explanation:

There are many different techniques to isolate bacteria populations. Using solid media such as agar, you can perform the streak for isolation technique.

What you want to do by using this technique is to get an isolated colony of the bacteria of interest. And to get it, you need to dilute a sample or inoculum of bacteria as much as possible by streaking it. Every time you do it, you take fewer amounts of cells from the original sample, separating them each time more, to the point of having only one single cell apart by a few millimeters from another cell. The isolated cell divides and forms a new pure colony.

So basically, you need to imaginary divide the agar plate into four quadrants.

You sterilize the loop carefully and take a sample of bacteria, trying to get the smallest amount of inoculum. You will apply the loop with the sample in quadrant one and spread it. You close the plate and turn it, placing the second quadrant in front of you. You sterilize the loop again on the flame and let it cool. You perform the same motion in quadrant two but carefully touching the edge of quadrant one, so you can take some of the cells that you have already spread on it. Once you spread quadrant two, you close the plate and turn it again, so quadrant three is in front of you. You sterilize the loop again on the flame and let it cool. You perform the same motion in quadrant three but carefully touching the edge of quadrant two. Close and rotate the plate. You sterilize the loop and repeat the same motion in the last quadrant. Close the plate and take it to incubation. Finally, you put the loop on the flame for the last time.The importance of extending the streaks into the anterior quadrant is to take a few cells from there and to spread them in the following quadrant. In this way, you are diluting the original inoculum, and separating the cells each time more. The fourth quadrant should have a few isolated cells that will reproduce and form a pure colony. If you do not touch the anterior quadrant while streaking, you will not have any cell in the quadrant, and you will not observe any colony after the incubation. The importance of sterilizing the loop every time you change the quadrant is to avoid contamination with other species. If you do not flame the loop, the result will be too many colonies of different species, probably touching themselves, in each quadrant.


Related Questions

Many scientists claim that the facts support the belief that climate change is really happening. By examining scientific research and evidence, you will better understand issues and cut through the myths. As you read in the unit, it is important to carefully consider evidence because there is a lot of information in the public sphere that looks like science but it is not based on the scientific method. For this activity, you are going to do your own research on global warming and sort out the facts for yourself. Do research online to find evidence about global warming. To find reliable information, be sure that you use reliable websites, such as those from government organizations (.gov) or universities (.edu). You can also look at publications that are reputable, such as Popular Science, Discover, or National Geographic. Find a minimum of three sources that provide evidence about global warming.

Answers

Climate change is strong evidence of global warming. The data maintained by the concerned departments shows that as the graph of global warming rose, adverse changes in the climate occurred.

Therefore, an increase in global warming and change in climate is directly related. The increase in the speed of the melting iceberg can be seen as the temperature or global warming has increased.

What are the effects of climate change?

Climate is a very important factor in the lives of all living organisms, because it is one of the major factors that determine if a living organism is going to survive or not.

Climate change is said to occur when there are changes in the earth's climate system, which result in the formation of new weather patterns that last for a long time.

Climate change affects living organisms in different ways; while climate change is beneficial to some organisms, it is detrimental to some. For instance, in Europe, climate change (increase in temperature) has been found to have positive effects on some bird populations.

Thus, this could be the answer.

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Photosynthesis occurs with the help of
A. CO2 and Water
B. SO2 and water
C. Oxygen and CO2
D. Oxygen and water​

Answers

Explanation:

(A) = Carbon dioxide and water. Because, photosynthesis is the process by which green plants manufacturer their food by combining water and carbon dioxide in the process of sunlight energy trapped by chlorophyll to produce carbohydrate and released oxygen. from the above definition we see that, Photosynthesis cannot occur without water and carbon dioxide present

Refer to the given diagram to answer Question 9
Y-ANTIBODIES
X-WBC
V-RBC
W-PLATELETS
QUESTION 9
ry
Which of the labelled structures in the diagram match the function below.
1.
Contains haemoglobin
II.
Carries out phagocytes
III.
Produces antibodies
J.
Involves in blood clotting
IV
A.
V
Y
W
В.
W
V
Y
С.
V
Y
X
w
D
Y
X
W​

Answers

Answer:

C

Explanation:

Answer c contains all the list listed

The differences in beak shape are examples of:

Answers

Answer:

BIOLOGICAL ADAPTATION

Explanation:

Bird beaks are textbook examples of BIOLOGICAL adaptation to diet, but their shapes are also controlled by genetic and developmental histories.

Answer: The first one is bacterial because it mutates very quickly compared to humans and the second one is biological adaptation

Hope it helps you if not sorry

Explanation:

eagles have specially adapted feet and talons to help them out. A series of bumps on the bottom of their feet -- known as spicules -- help them hold on to their prey during flight. They also have razor-sharp talons that they use to grab their prey, kill it and tear into.

In the presence of lactose, how long does it take for the lac operon to be expressed?
(A) when glucose is more than lactose concentration
(B) when lactose equals glucose concentration
(C) as long as lactose is more than galactose concentration
(D) as long as lactose is more than glucose concentration

Answers

Answer:

the lactose Expresses as long as it is present. when all lactose is converted into glucose and galactose, the reaction stops

Which is the word equation for photosynthesis?

Answers

Answer:

carbon dioxide + water + sunlight + chlorophyll → glucose + oxygen

Explanation:

I hope this helps in any way :)

The organ that controls your internal clock is called...​

Answers

Answer:

The circadian biological clock is controlled by a part of the brain called the Suprachiasmatic Nucleus

Explanation:

That should be right

what are the characteristics of normal cells and cancerous cells?​

Answers

Normal cells stop growing (reproducing) when enough cells are present. For example, if cells are being produced to repair a cut in the skin, new cells are no longer produced when there are enough cells present to fill the hole (when the repair work is done).

In contrast, cancer cells don’t stop growing when there are enough cells present. This continued growth often results in a tumor (a cluster of cancer cells) being formed.

what are the advantages of genetically modified plants​

Answers

Answer:

advantages are of genetically modified plants are the desired traits it results in but also they well-grown fruits and vegitables will be plump, ripe and delicious.

Explanation:

Which statement describes Mendel’s hypotheses regarding gametes?
A) gamete carries two genes for a trait.
B) gamete carries one allele for a gene.
C) gamete can carry multiple alleles for a trait.
D) Some gametes are dominant and some are recessive.

Answers

Answer:

The correct answer is B. A gamete carries one allele for a gene.

Explanation:

Gamete (also called sex cell or reproductive cell) is a haploid cell (having half number of chromosome as compared to the parent cell) that is produced through a cell division, termed as meiosis in sexually reproducing organisms.

According to the Law of segregation given by Gregor Mendel-

During the process of gamete formation, the two alleles of each gene segregate from one another so that a gamete carries one allele for a gene.

Thus, option B) is the right answer.

Answer:

B.

Explanation:

Which of the following is not true regarding the establishment of a neuron's resting membrane potential?

a. Resting membrane permeability to Na+ is very low.
b. Ion pumps in the plasma membrane eject sodium ions as fast as they cross the membrane.
c. Electrical forces do not push sodium ions into the cell.
d. Chemical and electrical forces both favor sodium ions entering the cell.
e. The chemical gradient for potassium ions tends to drive them out of the cell.

Answers

Answer:

c

Explanation:

c answer have a great day

trình bày cơ chế hấp thụ và chuyển hóa lipid từ đường tiêu hóa ở người?

Answers

Trong dạ dày được tách rời khỏi các chất thực phẩm khác. Trong ruột non, mật hoa tiết ra chất béo trong khi các enzyme tiêu hoá chúng. Các tế bào ruột hấp thụ chất béo. Các a - xít béo chuỗi dài tạo thành một cấu trúc lipoprotein lớn được gọi là một hạch nguyên tử có thể vận chuyển chất béo qua hệ bạch huyết.

science
the imaginary lines which divide the earth into areas of 15º are called

Answers

[tex]\huge\colorbox{purple}A\colorbox{blue}n\colorbox{purple}s\colorbox{blue}w\colorbox{purple}e\colorbox{blue}r [/tex]

[tex]\color{red}{⚠TheQueenNoNeedAKing⚠} [/tex]

[tex]\color{Violet}\rm{Answer:}[/tex]

longitude lines

longitude lines. Imaginary lines, also called meridians, running vertically around the globe. Unlike latitude lines, longitude lines are not parallel. Meridians meet at the poles and are widest apart at the equator. Zero degrees longitude (0) is called the prime meridian.

[tex]\color{Violet}\rm{☘︎Hopeithelps!}[/tex]

__________________________________

“But you, my Lord, are a God of compassion and mercy; you are very patient and full of faithful love.” — Psalm 86:15 ❤

__________________________________

23. Which of the following muscles make up the rotator cuft?
A Supraspinatus, infraspinatus, teres minor, subscapularis
B. Supraspinatus, teres major, teres pectoralis minor, subscapularis
C. Supraspinatus, infraspinatus, teres minor, levator scapulae

Answers

Answer:

A

Explanation:

together they assist in stabilizing the shoulder joint as well in performing various arm movements

It’s a but my bad if it’s no

Which of these is true for a fertilized egg?
O
A. It has DNA from both parents.
O B. It has a haploid number of chromosomes. .
O C. It has DNA but no chromosomes.
O D. It has too many chromosomes until it splits.

Answers

A it has dna from both parents

this is the answer for
'select the correct location on the image.

Where are the photoreceptors located inside a human eye?'

Answers

nice I'm going to do this with all the answers i have now

please help
Which part of the Sun can be seen only during a solar eclipse? O A. Chromosphere B. Convection zone O C. Corona D. Photosphere​

Answers

Answer:

chromosphere

Explanation:

Its the outmost layer of the sun, the only part that is visible on an eclipse.

Answer:

chromosphere

Explanation:

It is only visible during total solar eclipses or with sophisticated telescopes.

what are the three strategies that the community could place to work with governmental structures to stop illegal dumping​

Answers

Answer:

Illegal Dumping has gotten out of hand.  According to the Los Angeles County Public Works, “People usually dump illegally to avoid collection and disposal fees, or because they believe proper disposal is just ‘too much trouble’.”   This is very unfortunate for our communities who receive the negative consequences of higher taxes for cleanup, environmental hazards, and a breeding ground for rats.

The cost of a one-time cleanup and detoxing of an area can cost thousands of dollars.  But to leave the mess is just not an option.  The best option is to stop the illegal dumping before it happens, preventing the cost of cleanup, preventing the chemicals and toxins from entering the ground water, and preventing the rats and mosquitoes from breeding.  The best areas to live are communities that care about their community!  So, what can a community do to prevent illegal dumping?

Here are some key points to help deter illegal dumping:

Educate the public through the schools and through community awareness groups about the costs, chemical hazards and dangers incurred by illegal dumping

Create safe, public-friendly waste disposal options and advertise them

Create laws that impose stiff fines for the perpetrators and advertise these stiff fines

Use Illegal Dumping Cameras to catch the perpetrators in the act

Use Social Media to ask the public to identify the perpetrators

Follow through with quick clean up

These steps will invite the citizens to be involved and help deter the criminal activity of illegal dumping within your community.

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Please help!!

Why doesn't cloud formation take
place until the dew-point temperature is
reached?

Answers

Answer:

Clouds form when air reaches its dew point, the temperature when the air is saturated. This can happen in two ways. First, the air temperature can stay the same while the humidity increases. This is common in locations that are warm and humid.

Explanation:

Answer: The moisture in the cloud affects the weather.

Explanation:

thats why

From what you know of tonicity, what can you say about the plant cells and the solutions in the test tubes

Answers

Answer:

Tonicity is the relative amount of solutes that is dissolved in solution which determine the direction and level of diffusion.

Explanation:

Tonicity is the relative amount of solutes that is dissolved in solution which determine the direction and level of diffusion. There are three types of tonicity i.e. hypertonic, hypotonic and isotonic solution. If a cell is placed in a hypertonic solution, water will leave the cell, and the cell will shrink due to more solute in the external environment. In an isotonic environment, there is no water movement occurs, so there is no change in the size of the cell. When a cell is placed in a hypotonic environment, water will enter the cell, and the cell will swell. due to lower amount of water present inside the cell.

If a human has an X and a Y chromosome pair, they are

Answers

With this x and y pair they are male

difference between enzyme and inorganic catalysts​

Answers

Answer:

Enzymes catalyze a reaction which in presence of a catalyst proceeds the reaction faster. Inorganic catalysts promote a chemical reaction between many different reactants. Enzymes are proteinaceous in nature whereas inorganic catalysts are not proteinaceous in nature.

Explanation:

Shania has a family history of a genetically inherited disease. Based on this information, which statement is true? O Shania has an increased risk of the disease. Shania will get the disease at some point in her lifetime. O If Shania makes unhealthy choices, she will get the disease. The disease will be expressed in all her offspring that have that gene.​

Answers

Answer:

Shania will get the disease at some point in her life

Explanation:

this will happen because the sickness is genetically implanted in her family

Answer:

The diesease will be apart of the genes in her offspring.

Explanation:

Often times mothers with the sickness gene will spread down the gene therefore starting the generational infection in the first place.

………………………………………………………………….

Answers

whats with the dots ._.

The total magnification produced from a 15x ocular and a 4x objective would be

Answers

60. To get total magnification you simply multiply ocular by the objective.

1. Which of the following lists includes cellular structures found
in both plant and animal cells?
A. Chloroplasts, cell wall, cell membrane, cytoplasm
B. Vacuoles, cell membrane, nucleus, mitochondria
C. Cell membrane, cytoplasm, nucleus, cell wall
D. Chloroplasts, vacuoles, cell wall, cytoplasm

Answers

Answer:

C. Cell membrane, cytoplasm, nucleus, cell wall

Explanation:The plant cell and the animal cell can be differentiated by the presence of organelles in them.although both are classified as eukaryotes, the presence of the cell wall, vacuoles, and chloroplasts are the most remarkable and distinguishing components of the plant cells which are absent in the animal cells.

The process of photosynthesis ultimately converts light energy into -

Answers

Glucose is the answer.

15. The movement of molecules in a fluid or gas from a region of higher
concentration to a region of lower concentration is called:

Answers

Explanation:

in gases it is called diffusion:)

Which statement describes all stable ecosystems?
1) Herbivores provide energy for the autotrophs,
2) The populations of predators are dependent on
the populations of their prey.
3) The number of autotrophs equals the number of
heterotrophs.
4) Consumers synthesize ATP from light energy.

Answers

2. The populations of predators are dependent on the populations of their prey.

Stable ecosystem has the populations of predators are dependent on the populations of their prey of predators .

What is ecosystem?

An ecosystem is a geographic area where plants, animals, and other organisms, as well as weather and landscape, work together to form a bubble of life.

Who are predators ?

Predators are organisms that hunt and kill other organisms for food. The organisms that are consumed by the predators are known as the prey.

Stable ecosystem has the populations of predators are dependent on the populations of their prey of predators .

Hence, option 2 is correct

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If the velocity of a can increases from 30 ms-1 to 90 ms -1, the kinetic energy
increases by
a. 8 times
b. 9 times
c. 7 times
d. 6 times​

Answers

The answer to this one is 6 times.
Hello!

The right answer is 6times! Then D!

Byye and don’t forget I’m proud of you!!<333
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