Which of the following is a concern if you forgot to resuspend (mix) culture solutions when performing serial dilution?
A) Bacteria would no longer be able to grow on solid media
B) CFU/mL calculations would not be accurate
C) Colony morphology would be disrupte
D) The pure culture would be contaminated

Answers

Answer 1

Answer:

B) CFU/mL calculations would not be accurate

Explanation:

In microbiology CFU is the acronym for Colony Forming Unit, which indicates the number of bacteria or fungi capable of multiplying under suitable conditions. In this case, CFU/mL indicates how much bacterial or fungal cells, susceptible to reproduction, exist within 1 mL of a culture solution.

When this solution is used, to make a dilution or other laboratory activity, it is important that it be resuspended, that is, mixed, as the bacteria or fungus cells can accumulate in the bottom or surface of the substance, changing the value of CFU/mL and causing inaccurate dilutions if used. When resuspended, the substance presents the appropriate value of CFU/mL, ensuring that the dilution is accurate and effective.


Related Questions

When performing the streak for isolation technique, your result is unexpected: There is only growth obtained in quadrant 1. The growth observed in quadrant 1 is as expected, heavy with no isolated colonies. However, no growth is visible in any other quadrants.
Which of the following errors could explain this unexpected result?
A) The loop did not cross into quadrant 1 when streaking quadrant 2
B) You forgot to flame the loop in between quadrants
C) All of the errors listed here could be responsible for this unexpected result
D) Not enough bacteria was obtained from the starting culture when streaking quadrant 1

Answers

Answer:

A) The loop did not cross into quadrant 1 when streaking quadrant 2

Explanation:

The streak for isolation technique is a method used to get isolated bacterial colonies. This technique consists of, in a petri dish with culture medium, where, with the help of an inoculation loop, the bacteria are scratched on one side of the dish. This side is called the first quadrant. This inoculation loop must then cross with the first quadrant and be dragged to the second quadrant, where new lines must be made. This allows a part of the bacteria from the first quadrant to be carried over to the second. The inoculation loop must cross the second quadrant again to inoculate the third quadrant, and so on.

In this case, we can see that for bacterial colonies to grow in all quadrants, it is essential that the loop crosses in the first quadrant to cross out the second quadrant. If not, only the first quadrant will have bacterial colonies.

Answer:

A) The loop did not cross into quadrant 1 when streaking quadrant 2

Explanation:

There are many different techniques to isolate bacteria populations. Using solid media such as agar, you can perform the streak for isolation technique.

What you want to do by using this technique is to get an isolated colony of the bacteria of interest. And to get it, you need to dilute a sample or inoculum of bacteria as much as possible by streaking it. Every time you do it, you take fewer amounts of cells from the original sample, separating them each time more, to the point of having only one single cell apart by a few millimeters from another cell. The isolated cell divides and forms a new pure colony.

So basically, you need to imaginary divide the agar plate into four quadrants.

You sterilize the loop carefully and take a sample of bacteria, trying to get the smallest amount of inoculum. You will apply the loop with the sample in quadrant one and spread it. You close the plate and turn it, placing the second quadrant in front of you. You sterilize the loop again on the flame and let it cool. You perform the same motion in quadrant two but carefully touching the edge of quadrant one, so you can take some of the cells that you have already spread on it. Once you spread quadrant two, you close the plate and turn it again, so quadrant three is in front of you. You sterilize the loop again on the flame and let it cool. You perform the same motion in quadrant three but carefully touching the edge of quadrant two. Close and rotate the plate. You sterilize the loop and repeat the same motion in the last quadrant. Close the plate and take it to incubation. Finally, you put the loop on the flame for the last time.The importance of extending the streaks into the anterior quadrant is to take a few cells from there and to spread them in the following quadrant. In this way, you are diluting the original inoculum, and separating the cells each time more. The fourth quadrant should have a few isolated cells that will reproduce and form a pure colony. If you do not touch the anterior quadrant while streaking, you will not have any cell in the quadrant, and you will not observe any colony after the incubation. The importance of sterilizing the loop every time you change the quadrant is to avoid contamination with other species. If you do not flame the loop, the result will be too many colonies of different species, probably touching themselves, in each quadrant.

what are the three strategies that the community could place to work with governmental structures to stop illegal dumping​

Answers

Answer:

Illegal Dumping has gotten out of hand.  According to the Los Angeles County Public Works, “People usually dump illegally to avoid collection and disposal fees, or because they believe proper disposal is just ‘too much trouble’.”   This is very unfortunate for our communities who receive the negative consequences of higher taxes for cleanup, environmental hazards, and a breeding ground for rats.

The cost of a one-time cleanup and detoxing of an area can cost thousands of dollars.  But to leave the mess is just not an option.  The best option is to stop the illegal dumping before it happens, preventing the cost of cleanup, preventing the chemicals and toxins from entering the ground water, and preventing the rats and mosquitoes from breeding.  The best areas to live are communities that care about their community!  So, what can a community do to prevent illegal dumping?

Here are some key points to help deter illegal dumping:

Educate the public through the schools and through community awareness groups about the costs, chemical hazards and dangers incurred by illegal dumping

Create safe, public-friendly waste disposal options and advertise them

Create laws that impose stiff fines for the perpetrators and advertise these stiff fines

Use Illegal Dumping Cameras to catch the perpetrators in the act

Use Social Media to ask the public to identify the perpetrators

Follow through with quick clean up

These steps will invite the citizens to be involved and help deter the criminal activity of illegal dumping within your community.

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what is difference between biology and chemistry​

Answers

biology is about cells and the body

chemistry is more elements and molecules

Answer:

Biology is the study of life or to precise, living organisms and how they react whilst chemistry is the study of the reaction of substances and their properties.

Explanation:

Biology is more of organisms like humans and how they react to things

Chemistry is more of how substances react and their properties

what system produces most of the hormones in the human body

Answers

The hormones created and released by the glands in your body's endocrine system control nearly all the processes in your body

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Answer:

The endocrine system.

Explanation:

It's a group of glands that produce and secrete hormones that the body uses for a variety of purposes.

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what is a reasonable measurement for the distance across the milky way galaxy ?

Answers

Answer:

Between 100,000 and 150,000 light-years

Explanation:

According to BBC, "the Milky Way Galaxy is probably between 100,000 and 150,000 light-years across"

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1
Cellular Respiration
son
What is the purpose of cellular respiration? How does it occur?

Answers

Answer:

Cellular respiration, the process by which organisms combine oxygen with foodstuff molecules, diverting the chemical energy in these substances into life-sustaining activities and discarding, as waste products, carbon dioxide and water.

Explanation:

Answer:

The main function of cellular respiration is to synthesize biochemical energy. Cellular respiration is essential to both eukaryotic and prokaryotic cells because this biochemical energy is produced to fuel many metabolic processes, such as biosynthesis, locomotion, and transportation of molecules across membranes.

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Which of the following statements about the use of synthetic polynucleotides in cracking the genetic code is true?
a) Particular codons are generated in predictable ratios with random RNA polymers containing specific ratios of different nucleotides.
b) Poly(A) RNA directs the synthesis of only polylysine in vitro.
c) Three different amino acids are incorporated into peptides when synthetic polymers of only one nucleotide are used to synthesize the RNA primer.
d) The codon table was completed by using RNA polymers of defined sequences.

Answers

Answer:

a) Particular codons are generated in predictable ratios with random RNA polymers containing specific ratios of different nucleotides.

b) Poly(A) RNA directs the synthesis of only polylysine in vitro.

d) The codon table was completed by using RNA polymers of defined sequences.

Explanation:

A polynucleotide molecule is defined as the biopolymer which is composed of thirteen nucleotide monomers or more monomers which is covalently bonded in the chain.

While using the synthetic polyneuclitide in the cracking of the genetic code, the translation of a [tex]\text{poly(A) RNA tail}[/tex] that results in synthesis of the poly-lysine in the lab.

Some of the particular codons are produced by some specific ratios of a different nucleotides. This can be present in the different RNA polymers. Codon table includes the RNA polymers that contains nucleotides in some specific ratios.

Thus, the options (a), (b) and (d) are correct.

Reproduction is important for every type of living thing. Without it, species would die out. To ensure their survival, animals have developed unique adaptations to improve their chances of reproducing. Behavioral adaptations are behaviors an organism performs to help it survive. Structural adaptations are physical features that help an organism survive.

The image below shows two peacocks. The male peacock is large and brightly colored. The female peacock is not as colorful. The size and color of the male's feathers attract females. This is an example of a structural adaptation intended to help with reproduction. Another structural adaptation is when male deer grow large antlers to attract females. Animals also use behavioral adaptations to attract mates. For example, male frogs sing to attract females and male spiders dance to attract females.



Plants also have adaptations to help with reproduction. While animals use behavioral and structural adaptations to improve reproduction, plants only use structural adaptations. For example, brightly colored flowers attract butterflies. Certain flowers also have nectar or give off an odor to attract insects. Attracting butterflies and other insects is important because they play a role in reproduction by pollination.

Which of the following describes an adaptation that relates to reproduction?

A. Hawks have sharp beak to help them quickly catch their prey
B. Elks attach leaves and grasses to their antlers to help them appear larger
C. Polar bears have white fur to help them blend into their surrounding environment
D. Whales have a thick layer of fat called blubber

Answers

Answer:

B. Elks attach leaves and grasses to their antlers to help them appear larger

Explanation:

The above option is the correct answer.

As elks appear larger, it tend to make other male elks to be scared to fight that particular elk for a female elk. Also, the female elk becomes more attracted to larger male elk.

Male elk are known to actually retain their antlers for more than half the year. Such elks do not likely group themselves with other males when they have antlers.

Small, reduced leaves with a single vein occurring in the club mosses

Answers

Answer:Club mosses are low evergreen herbs with needlelike or scalelike leaves. Many species have conelike clusters of small leaves (strobili), each with a kidney-shaped spore capsule at its base. The plants are homosporous, meaning they produce just one kind of spore

Explanation:Although they lack true veins, many species of mosses have long narrow cells in their stems, the midribs of their leaves, and their rhizoids (root-like plant tissue) that can be considered evolutionary precursors to true veins. Vascular plants with true veins include the clubmosses, ferns, and flowering plants.

can someone help me with this question?

Answers

Answer:

research question: how do different humidity levels affect the heights of different types of trees

independent variable: humidity level

dependent variable: tree height

constants: temperature, light, duration of time

control group: tree left outside in normal conditions

Explanation:

8. Why are the proteins produced by a GMO crop monitored and evaluated? O A. To determine whether the organism will cause an allergic reaction O B. To evaluate whether the Bt toxin is produced O C. To measure hormones present in dairy products O D. To examine the need for additional fertilizers​

Answers

Answer:

 C. To evaluate whether the Bt toxin is produced

Explanation:

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To determine whether the organism will cause an allergic reaction.

Proteins have roles as toxins, anti nutrients, or allergens, which have great impact on human health.

What is GMO?A genetically modified organism (GMO) is an animal, plant, or microbe whose DNA has been altered using genetic engineering techniques.Bio fortified GM crops have been adopted, increasing micronutrient availability.GMO foods are as healthful and safe to eat as their non-GMO counterparts.Genetically modified organisms (GMOs) are produced using scientific methods that include recombinant DNA technology and reproductive cloning.

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Which lists the correct order of evolutionary history?
crabs, jellyfish, reptiles
mammals, amphibians, crabs
vertebrate fish, crabs, mammals
jellyfish, reptiles, mammals

Answers

Answer:

jellyfish, reptiles, mammals

Explanation:

I took a test on edg in 2021

The lists in the correct order of evolutionary history is jellyfish, reptiles, mammals.

What is Evolution?

This is the process which involves the modification of structures in

organisms as a result of natural selection thereby making them more fit in

the environment.

The jellyfish has less complex and specialized and mammals has the most

which is why option D is the most appropriate.

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What does a chromosome consist of?

A. Tightly wound DNA only.

B. Loosely dispersed DNA and proteins.

C. Tightly wound DNA and proteins.

D. Loosely dispersed DNA.

Answers

I think C, but I’m not sure.

Answer:

C

Explanation:

So, there are 2 different forms that DNA can be found in.

The more common form, where the DNA is loosly scattered.

There is a less common form, however, only found during cell division, which is a compacted form. The DNA is tighly wound in this case.

In the case of chromosomes, DNA is tightly pakced, and not loose.

Now, do they are do they  not have protiens?

Well, heres another question, how does the DNA get compacted?

It has to wrap around histone protiens.

The logn strands of DNA wrap around many histone protiens, until the full length of DNA has been compacted.

So to answer the question, yes, there are protiens found in chromosomes(specificly histone).

This means the answer must be C, since chrosomes are both tightly bound, and contain protein.

Which of the following are examples of natural pollution? Choose all that apply.

car exhaust
volcanoes
pesticides
trash
forest fires
floods

Answers

car exhaust
pesticides
trash
forest fires

The examples of natural pollution are Volcanoes, Forest fires, and floods.

What do you mean by Pollution?

Pollution may be defined as the presence of any unwanted or contaminated substances in the atmosphere.

Car exhaust, pesticides, and trash are examples of artificial pollution.

Volcanoes release certain poisonous gases which are harmful to environment. Flood carries contamination like pesticides, chemicals, sewage waste, etc.

Forests fire are responsible for the increase of carbon dioxide in the environment.

Therefore, examples of natural pollution are Volcanoes, Forest fires, and floods.

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The movement of organic materials(food) takes place through
A. Xylem
B. Vessels
C. Tracheids
D. Phloem​

Answers

Explanation:

everything can be found in the picture

the correct answer is (D) = phloem

Please help!!

Why doesn't cloud formation take
place until the dew-point temperature is
reached?

Answers

Answer:

Clouds form when air reaches its dew point, the temperature when the air is saturated. This can happen in two ways. First, the air temperature can stay the same while the humidity increases. This is common in locations that are warm and humid.

Explanation:

Answer: The moisture in the cloud affects the weather.

Explanation:

thats why

difference between enzyme and inorganic catalysts​

Answers

Answer:

Enzymes catalyze a reaction which in presence of a catalyst proceeds the reaction faster. Inorganic catalysts promote a chemical reaction between many different reactants. Enzymes are proteinaceous in nature whereas inorganic catalysts are not proteinaceous in nature.

Explanation:

what level of biological organization is illustrated by the image? click the correct box​

Answers

Answer: Ecosystem

Explanation: This image shows multiple species interacting with their environment: human and rabbit within waterfall and tree.

The level of biological organization that is illustrated by the image is called Ecosystem.

An ecosystem is defined as a self supportiung unit that is made up of a living part (biotic factors) like plants and animals; and non-living part (abiotic factors) like water, soil, air.

In an ecosystem, the biotic community includes population of:

Food producers: That is the autotrophs like the green plants which are seen in the attached diagram.

Food consumers : That is the heterotrophs such as animals and human beings.

The non-living part of an ecosystem is its abiotic component. this consist of:

Abiotic resources: These are what organisms need to stay alive. they include a source of energy (sunlight as seen in the diagram) and inorganic nutrients ( carbon dioxide, water).

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Which of the following is not true regarding the establishment of a neuron's resting membrane potential?

a. Resting membrane permeability to Na+ is very low.
b. Ion pumps in the plasma membrane eject sodium ions as fast as they cross the membrane.
c. Electrical forces do not push sodium ions into the cell.
d. Chemical and electrical forces both favor sodium ions entering the cell.
e. The chemical gradient for potassium ions tends to drive them out of the cell.

Answers

Answer:

c

Explanation:

c answer have a great day

A traumatic injury to the inferior angle of the mandible severs the hypoglossal nerve. Which of the following muscles would be affected?

a. Geniohyoid
b. Mylohyoid
c. Genioglossus
d. Stylohyoid

Answers

C, genioglossus is the answer :)

The muscle that would be affected is the Genioglossus. That is option C.

The hypoglossal nerve is the also known as cranial nerve XII.

This nerve arises from the nuclei in the medulla oblongata.

They supply:

the muscles of the tonguemuscles surrounding the hyoid bone andcontribute to swallowing and speech.

The muscles of the tongue  includes

genioglossus, hyoglossusstyloglossus, and palatoglossus

The genioglossus is a flat triangular muscle that forms the base by which the tongue is attached.

Since the hypoglossal nerve supplies the the tongue muscle (genioglossus), it would be must affected when mandible severs the hypoglossal nerve.

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How much stronger is a magnitude 8 earthquake than a magnitude 5 earthquake?

A. 1000
B. 30
C. 3
D. 10

Answers

Answer:

A. 1000

Explanation:

The Richter scale is a scale of numbers used to measure the magnitude of an earthquake, which is based on the size of its seismic waves. This scale indicates a number to quantify the total seismic energy released by an​ earthquake. The Richter magnitude scale is a base-10 logarithmic scale, thereby a difference of one (1) point in the scale of magnitude corresponds to a 10-fold increase in amplitude. For example, based on the Richter scale, a magnitude-8 earthquake is 100 times greater than a magnitude-6 and 1000 times greater in amplitude than a magnitude-5.

three constitutional limitations to the right to freedom of expression?​

Answers

Answer:

(1)limitation must be within the constitutional power of government; (2) the limitation must further an important or substantial governmental interest; (3) the governmental interest must be unrelated to the suppression of the freedom of expression; and

Synovial joints have the greatest range of flexibility and motion.


Please select the best answer from the choices provided.

T
F

Answers

Answer: The answer is true

Answer:

true

Explanation:

true

A zygote is best described as
A. a blastocyst attached to the placenta through the uterus
B. a sperm or an egg
C. any haploid cell
D. the first cell with DNA from both parents in its nucleus

Answers

Answer:

A zygote is D. The first cell with DNA from both parents in it’s nucleus.

Explanation:

A zygote is the unison of a sperm and an egg cell as to where the genetic information is combined in the fusion of the two parents haploid cells. Therefore it would be the first cell that contains DNA in the nucleus from both parents.

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what kind of molecule is represented in the diagram?

Answers

Answer:

The answer is D, a nucleotide

Explanation:

Answer:

A carbohydrate or (carb)

Explanation:

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Which clastic sedimentary rock is made of the smallest grains?

1) Conglomerate
2) sandstone
3) siltstone
4) shale

Answers

Answer:

Shale

Shale has the smallest grain size. Shale is made mostly of clay-sized particles and hardened mud.

Explanation:

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The branch shown below has FOUR individual leaves. Use the dichotomous key to identify the tree’s genus and species.
A.Albizia julibrissin
B.Cornus florida
C.Juglans nigra
D.Pinus taeda
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Answers

The answer is c Bc Linus taeda

From what you know of tonicity, what can you say about the plant cells and the solutions in the test tubes

Answers

Answer:

Tonicity is the relative amount of solutes that is dissolved in solution which determine the direction and level of diffusion.

Explanation:

Tonicity is the relative amount of solutes that is dissolved in solution which determine the direction and level of diffusion. There are three types of tonicity i.e. hypertonic, hypotonic and isotonic solution. If a cell is placed in a hypertonic solution, water will leave the cell, and the cell will shrink due to more solute in the external environment. In an isotonic environment, there is no water movement occurs, so there is no change in the size of the cell. When a cell is placed in a hypotonic environment, water will enter the cell, and the cell will swell. due to lower amount of water present inside the cell.

What are the parts of the chloroplast? What does each part do?

Answers

The chloroplast has an inner and outer membrane with an empty intermediate space in between. Inside the chloroplast are stacks of thylakoids, called grana, as well as stroma, the dense fluid inside of the chloroplast. These thylakoids contain the chlorophyll that is necessary for the plant to go through photosynthesis.

The signs used depend on the species being surveyed and include the presence of runways and tracks on *
1 point
(a). soft substrates
(b) hard substrate
(c) dry mud
(d) rocks

Answers

Answer:

(a). soft substrates

Explanation:

Mobile organisms can be measured by both direct and indirect methods. Direct methods may involve observing the actual behavior (e.g., scoring via timing). Moreover, indirect methods are those where the technique used depends on the species being examined, collecting information such as through recording of tracks in the soft substrate (e.g., mud, sand, snow), presence of fur/feathers, droppings (mammals), etc. For example, indirect observation of insects may involve the determination of exoskeletons, sounds, etc.

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