You plan to clone a guide RNA sequence into the pML104 plasmid because you want to engineer the construct in bacteria and later express it in yeast. Unfortunately, your lab does not have any available pML104. Your friend in the lab next door lets you borrow a related plasmid pML107, and you quickly start your experiment. Will it work? Briefly explain.

Answers

Answer 1

Answer:

Yes, the experiment will work.

Explanation:

The reason behind this is that plasmids are well known to be DNA vector carriers. These plasmids have the ability to bring about changes in DNA fragments into various microorganisms and reveal them. The two plasmids compose of the same genomic marker such as the antibiotic resistance gene found in URA3. However, plasmids are most commonly found in bacteria, but also in eukaryotic cells. Oftentimes, the gene composition in the plasmids enhances the bacteria with a vantage edge such as antibiotic resistance.

Hence, it is obvious that in both, the molecular regulation in terms of expression of genes is alike in properties. Similarly, the numbers of copies and molecular weight are also the same.

We can thereby conclude that, provided that there is a proper restriction site, another host that is up to standard for plasmid can be replaced for each other.


Related Questions

The color of a light wave is determined by its wavelength. On the COLOR tab, slowly drag the Light wavelength slider back and forth and observe the effect on oxygen production. How does the color of light affect the rate of photosynthesis

Answers

Answer:

The color of light determines how effective chlorophyll pigment will be at absorbing light.

Explanation:

Photosynthesis is a metabolic process that requires light, which is an electromagnetic radiation. Light on the electromagnetic spectrum is determined by wavelength. Each region/color of light occurs at a specific wavelength.

Hence, chlorophyll pigment, which is the major photosynthetic pigment effectively absorbs blue and red light in the visible light region of the electromagnetic spectrum. Hence, the rate of photosynthesis will be affected negatively if the color of light is not the one stated above.

Which of the following statements is true?
OA. Chemical reactions can either absorb thermal energy or release thermal energy
ОВ.
Chemical reactions can only absorb thermal energy.
OC.
Chemical reactions can neither absorb thermal energy nor release thermal energy.
OD.
Chemical reactions can only release thermal energy.

Answers

I think the answer is A! i might be wrong

Answer: Chemical reactions can either absorb thermal energy or release thermal energy.

Use the following table to answer the question:


Element

Mass Number

Atomic Number

Aluminum

27

13

Nitrogen

14

7

Helium

4

2

Fluorine

19

9

please help

Which element has the least neutrons in the nucleus?
Aluminum
Nitrogen
Helium
Fluorine

Answers

Answer:heh

Explanation:

Aluminium , mass no = no of proton + neutron = 27

atomic no = no of proton = 13

no of neutron = 27 - 13 = 14

Nitrogen  , mass no = no of proton + neutron = 14

atomic no = no of proton = 7

no of neutron = 14 - 7 = 7

Helium   , mass no = no of proton + neutron = 4

atomic no = no of proton = 2

no of neutron = 4 - 2= 2

fluorine    , mass no = no of proton + neutron = 19

atomic no = no of proton = 9

no of neutron = 19-9 = 10

Answer:

helium

Explanation:

i took the test and got it right... it also has the smallest number

What causes the earths tides; describe the conditions for the largest tides?

Answers

Answer:

Earth's rotation and the gravitational pull of the sun and moon create tides on our planet. Because the sun is so much larger than the moon (27 million times more masssive), it has a much bigger graviational pull on Earth.

Sickle cell anemia and albinism are both recessive traits in humans. Imagine that a couple, already pregnant with fraternal twins, has just learned that they (the couple) are both heterozygous for both of these traits. As the couple's genetic counselor, the couple asks you what is the probability of having both of the twins be albino and have sickle-cell anemia.

Answers

Answer:

81/256

Explanation:

By doing a test cross of albinism and sickle cell anemia you'll see that the probability that one child is unaffected by either conditions will be 9/16. Both children would be 9/16 * 9/16.  

Since fraternal twins are developed from two separate fertilization events the probability chance will be independent.

How many molecules of water are created when making a lipid?

Answers

Answer:

Three molecules

Explanation:

Since fats consist of three fatty acids and a glycerol, they are also called triacyclglycerols or triglycerides. Triacyclglycerols: Triacylglycerol is formed by the joining of three fatty acids to a glycerol backbone in a dehydration reaction. Three molecules of water are released in the process.

Answer: 3 molecules

Explanation: Hope that helps

If I put down an apple next to a banana will it ripen faster?

Answers

Answer:

no

Explanation:

when you move your hand up and down while holding on a jump rope, you make a wave. What is the medium

Answers

Answer:

The medium of the wave is the Rope.

Explanation:

This is because it is transverse wave and transverse wave need a medium to transfer energy and this is because in transverse wave,particles of the medium move in a direction that is perpendicular to the direction of the transverse wave. From the question, when you move you hand up and down, you make a wave and the direction of the wave is also up and down which is perpendicular to the direction of the wave.

What is the role of helicase in DNA replication?

Answers

Answer:

The helicase is essential because they separate the double-stranded DNA into single strands so that it could be copied.

Hoped this helped!

please help asap 10 points screenshot attached

Answers

i did this question in my biology class. ill text u the answers

(for anyone confused, ik him irl)

have a good day :)

HELP PLEASE DO THE WHOLE PAGE

Answers

Answer:

'krf'klmf

Explanation:

lkn4f4klng

Girl no that’s to much

1. Which of the following is NOT a characteristic of life.
A. Being Multicellular
B. Responsiveness to the Environment
C. Growth and Change
D. Maintain Homeostasis

Answers

Answer:

it may be a or d but b and c are a characteristic in life

A scientist wants to monitor the amount of acid
precipitation falling into a watershed.
Which tool would be the best Tor the scientist to use?
O A. pH meter
O B. Dissolved oxygen sensor
O C. Satellite images
O D. Bioindicators

Answers

Answer:

A. pH meter

Explanation:

This is because a pH meter measures the acidity of water.

Answer:pH meter

Explanation:

True or False: Chromosomes can be seen with the naked eye.


Answers

the answer is false Because it is so thin, DNA cannot be seen by the naked eye unless its strands are released from the nuclei of the cells and allowed to clump together.

which of the following is necessary for cellular respiration and photosynthesis to occur

Answers

Answer:

Sunlight!

Explanation:

A Class-A 10 mL pipette is supposed to dispense 10.00 mL of water. A lab technician makes three measurements of the volume that the pipette actually dispenses, and obtains volumes of 9.70 mL, 9.69 mL, and 9.71 mL. The pipette is:__________
a) Accurate, but not precise
b) Precise, but not accurate
c) Accurate and precise
d) Neither accurate or precise

Answers

Answer:

d) Neither accurate or precise

Explanation:

Accuracy and precision are critical factors in the experimental procedure. Accuracy can be defined as the amount of uncertainty in the process of measurement in relation to the true value (in this case, 10 mL). On the other hand, precision is an independent feature of the accuracy which indicates the repeatability of the measurement process (in this case, three measures weren't repeated). Therefore, precision indicates the confidence of a particular measurement, while accuracy indicates how close is this value to the real value.

Place the following events in
the cell cycle in the proper
order:
a. Anaphase
b. Cytokinesis
C. G2
d. Telophase
e. G1
f. Prophase
8. S
h. Metaphase

Answers

Easy: G1 stage, G2 stage, S phase, prophase, metaphase, anaphase, telophase and lastly cytokinesis

The cell cycle in the correct order is G1, S, G2, Prophase, Metaphase, Anaphase, Telophase, and Cytokinesis.

What is the cell cycle?

Prophase, metaphase, anaphase, and telophase are the four phases that a cell goes through. To form chromosomes, chromatin condenses (sister chromatids joined at the centromere).

In prophase, the chromosomes get shorter and thicker, in the center of the cell, during the metaphase, the chromosomes align. Chromatids separate during the anaphase process and travel to the opposing poles.

Telophase: Two nuclei are produced when nuclear envelopes have reformed around each chromosomal group.

Therefore, the cell spends the majority of its time in interphase, which is also when it grows, duplicates its chromosomes, and gets ready to divide.

Learn more about cells, here:

https://brainly.com/question/1600165

#SPJ2

Through which of the following processes are plant communities taking over the ecosystem

Answers

Answer:

Climate disturbance

Explanation:

3.4 Briefly describe the purpose of conjugation in paramecia.

Answers

Answer:

Conjugation is a process of exchange of genetic material between two organisms. paramecium is a unicellular protozoan in which true sexual reproduction is absent. so in these organisms conjugation brings genetic variatin in new generations.

Explanation:

The function of conjugation in paramecium is to exchange genetic material between organisms and make new combinations of genetic material.

Where does the water that a land plant uses for photosynthesis come from? The water comes from _____.


-Evaporation
-Condensation
-Precipitation

Answers

Answer:

precipitation

Explanation:

What would the molecular formula be for the following? For each answer, state what kind of bonds hold the molecule together (ionic, polar-covalent, or non-polar covalent)
a. Flourine reacting with sodium
b. Oxygen reacting with carbon
c. Nitrogen reacting with hydrogen
d. Silicon reacting with hydrogen
e. Sulfur reacting with hydrogen
f. Magnesium reacting with oxygen

Answers

Answer:

Explanation:

A. sodium fluoride = NaF = ionic bond

B. carbon dioxide = CO2 = polar covalent bond

C. hydrogen nitrate = 2NH3 = ionic bond

D. silicon hydride = Si nH 2n+2 = polar covalent bond (i think.. could be non-polar but definitely not ionic)

E. hydrogen sulfide = H2S = non-polar covalent bond

F. magnesium oxide = MgO = ionic bond

hope this helps! :)

unlike eukaryotes prokaryotes do not contain a system of internal membranes. Any organelles, proteins, and genetic material are free - floating within the cytosol. Select the description below that correctly identifies how protein synthesis differs in prokaryotic and eukaryotic cells as a result of eukaryotic compartmentalization.

A. RNA processing occurs outside of the nuclear membrane in prokaryotes and inside it in eukaryotes.

B. Translation can occur before transcription in a prokaryote but not in a eukaryote.

C. Transcription and translation can occur simultaneously in a prokaryote but not in a eukaryote.

D. Ribosomes are needed for translation of mRNA in eukaryotes, but they are not needed in prokaryotes.

Answers

Answer:

The difference in protein synthesis in prokaryotic and eukaryotic cells, due to the compartmentalization of the latter, is that transcription and translation can occur simultaneously in a prokaryote but not in a eukaryote (option C).

Explanation:

Eukaryotic cells are characterized by a true nucleus, surrounded by a nuclear membrane. Since transcription occurs in the nucleus and translation for protein synthesis occurs in the cytoplasm, the former process occurs before the latter.

In prokaryotic cells the absence of a nucleus and compartmentalization system makes it possible for transcription of DNA into RNA and translation to occur simultaneously.

The other options are not correct because:

    A. RNA processing, in both prokaryotes and eukaryotes, occurs in the cytoplasm.

    B. Transcription precedes translation in all organisms.

    D. Ribosomes are present and are necessary for protein synthesis in both prokaryotes and eukaryotes.

Describe how the gonorrhoea pathogen causes disease after it enter the body

Answers

Answer:

Gonorrhea is transmitted through sexual contact with the penis, vagina, mouth, or anus of an infected partner. Ejaculation does not have to occur for gonorrhea to be transmitted or acquired. Gonorrhea can also be spread perinatally from mother to baby during childbirth.

Compare the reactants and products in the chemical equations below. Which statement best describes the relation between matter and energy?

Answers

Answer and Explanation:

Chemical reactions are very complex processes which include the collision of molecules. During the chemical reactions, bonds between the breakdown of the atom and transformed in new ways. The chemical compounds that react together during the chemical reactions are called reactants, shown on the left side of the equation. They are broken down during the reaction and used as the reaction progresses. The chemical compounds that are formed from the rearrangement and breakdown of the reactants are called products. The products are shown on the right side of the chemical equation. As compare to the reactants, the products are more stable molecules. For example, in the reaction between H2SO4 and Zn, these two chemicals are the reactants, while ZnSO4 and H2 are the chemical reaction products.

                                                Zn + H2SO4       ZnSO4 + H2

                                                 (Reactants)            (Products)

The matter is a material that everything is made of matter. A property that matter has is known as energy. The same quantity of matter can have a diverse quantity of energy. Both energy and matter are identical. For example, if we add some energy into an ice cube, it will turn into liquid. After that, if we add more energy, liquid will turns into steam.

17. Which of these processes is the result of the motion of particles in a gas or liquid?
A. conduction
B convection
C. insulation
D. radiation

Answers

Answer:

B

Explanation:

Example on one of the 3 laws of motion

Answers

Answer:

Newton's First Law of Motion

Explanation:

A body at rest persists in its state of rest, and the body in motion remains in uniform motion along a straight line unless acted upon by a net external force.

2. Insects which attack woollen clothes are

a. Silkworm b. silver fish c. ants d. butterflies​

Answers

Answer:

a

Explanation:

it would be a silkworm because they feed exclusively on animal fibers such as wool. hope this helps!

It would be silkworm

which of the following statements about the cells of any multicelluar organisms is true

Answers

Answer The DNA in a cell is all the same

Explanation:

When human undergo anaerobic respiration , they

Answers

When humans and other animals lack sufficient oxygen, they'll create ATP through a form of anaerobic respiration that produces lactic acid as a byproduct. ... Yes 'Anaerobic' means 'without oxygen. Cells will produce ATP through lactic acid fermentation only under when they lack sufficient oxygen.

Answer:

They experience sore muscles

Explanation:

Have a nice day lol <3

You are monitoring Birch Lake,

Answers

A. Snails and crayfish would not survive in Birch Lake at a pH of 6.2.

B. Once they die out, the walleye will disappear as well.

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