You prepare a gel mobility assay with the following samples:

Lane 1 Radiolabeled lacO DNA + Lac Repressor protein preincubated with allolactose
Lane 2 Radiolabeled lacO DNA + Lac Repressor protein preincubated with lactose
Lane 3 Radiolabeled lacO DNA + Lac Repressor protein missing its DNA binding domain
Lane 4 Radiolabeled lacO DNA + Lac super-repressor (Is) preincubated with allolactose
Lane 5 Radiolabeled lacO DNA + lac repressor protein

On which lanes do you expect to see two bands?

a. 3
b. Lane 1 and 2
c. Lane 2, 4, and 5
d. 2 and 4
e. 1 and 5

Answers

Answer 1

Answer:

c. Lane 2, 4 and 5.

Explanation:

Two bands will be seen when a fraction of DNA added to gel is bound by Lac repressor protein. Radiolabeled LacO DNA will have two bands. Lac repressor is encoded with lacl gene. It has binding ability due to allolactose formation.


Related Questions

Which of these organelles prepares proteins to be fully functional?

A. Rough endoplasmic reticulum

B. Large vacuoles

C. Smooth endoplasmic reticulum

D. Chloroplasts

Answers

Rough endoplasmic reticulum

Answer:

Rough endoplasmic reticulum is among the organelles that are found in eukaryotic cells. The reason as to why it is called rough is because it has ribosomes attached to its surface. Its main function is to produce proteins.

Explanation:

the phenomenon is called a revision why did it happen

Answers

Answer:

help  in studying

Explanation:

Which critter trait is affected by an environmental factor, such as light, temperature, or
diet? Explain.

Answers

Answer:

Tallness.

Explanation:

Tallness is the critter trait that is affected by an environmental factor, such as light, temperature, or  diet because the organism remains short if they are not getting food properly as well as temperature. Food provides essential nutrients that contribute in the growth of organism. Suitable temperature of the body allows the different functions in the body to be done which directly affects the growth of the body and the organism becomes taller.

Viral or bacterial germs that invade body cells are called _____.


phagocytes


pathogens


white blood cells


lymphocytes

Answers

White blood cells is the answer

PNS axons are vulnerable to cuts, crushing injuries, and other types of trauma. However, a damaged ____________ can regenerate if the ____________ remains intact and a critical amount of ____________ remains. The success of peripheral nervous system axon regeneration depends upon two primary factors: (1) the amount of ____________ and (2) the ____________ between the site of the damaged axon and the ____________ . The possibility of repair is ____________ with an increase in either of these two factors. Neurolemmocytes play an active role in regeneration

Answers

Answer:

The correct answer is - damaged, effector organ, neurilemma, damage, distance, cell body, decreased.

Explanation:

PNS axons are prone to cuts, and other types of injuries. A damaged axon can regenerate if the effector organ remains intact and a critical amount of neurilemma is found.

The degree of success of these axons regeneration depends upon factors that are (1) the amount of damage, and (2) the distance between the site of the damaged axon and the cell body it innervates The possibility of repair is reduced with an increase in either of these two factors. Neurolemmocytes play an active role in regeneration

state 2 function of the kidney

Answers

ʕ•ﻌ•ʔ[tex]\huge\bold\pink{hello!!!}[/tex]ʕ•ﻌ•ʔ

HERE IS UR ANSWER

_____________________

The kidneys are powerful chemical factories that perform the following functions:

remove waste products from the body.remove drugs from the body.balance the body's fluids.release hormones that regulate blood pressure.produce an active form of vitamin D that promotes strong, healthy bones.
It removes waste products from the body. And also removes drugs from the body

are amino acids associated with proteins

Answers

Answer:

Yes

Explanation:

Amino acids create proteins

yes they are building blocks of proteins

Why do you think type 2 diabetes occurs so much more often than other inheritable diseases? Propose a hypothesis for why this allele has not been eliminated by natural selection.
Which type of species best describes a mouse: opportunistic or equilibrium? Explain why a mouse or rat population would continuously grow while other mammals (cheetahs) are nearly extinct.

Answers

Answer:

This allele has not been eliminated by natural selection because it may confer an adaptive advantage for particular environmental conditions  

Mice and rats are opportunistic species. These species can rapidly adapt to different environmental conditions, while cheetahs are unable to adapt to changing environments.  

Explanation:

An allele is a gene variant that may be beneficial, neutral, or deleterious for a particular environment. Different alleles are not eliminated by natural selection against other alleles (even when a priori seem to be deleterious) because they confer a selective advantage in a given environment. For example, the allele for sickle cell anemia is caused by a point mutation which is lethal in homo-zygous individuals, but heterozygous individuals are less likely to die of malaria, thereby this allele has been positively selected in geographic areas where malaria is an endemic disease. Moreover, mice and rats are opportunistic species, i.e., they are species that rapidly adapt to varied living conditions. These species are characterized to have high growth rates and produce many offspring. On the other hand, the cheetah is a highly specialized species that is unable to adapt to changing environments. In consequence, cheetah populations are more prone to become extinct than rat and mice populations.

Which of these statements are true about diffusion

A. It is how gas exchange occurs in the blood cells in the circulatory system

B. It is when particles move from an area of low concentration to high concentration

C. It is how white blood cells travel around in the body to find viruses and bacteria

D. It is how sunlight gets absorbed into chloroplast in plants for photosynthesis

Answers

Answer:  c

Explanation:

A certain animal species has either curly tails (C) or straight tails (c) and either hazel eyes (H) or blue eyes ( h). What are the possible genotypes of an animal with curly tails and blue eyes

Answers

Answer:

CChh

Cchh

Explanation:

Cc or CC can be a genotype for curly tails because both have a dominant allele for curly tails

hh can be a genotype for blue eyes because there are two recessive alleles for blue eyes that are present

In which of the scientific classifications are all the
animals grouped

Answers

The lion belongs to the following groups: Kingdom Animalia (includes all animals) Phylum Chordata (includes all vertebrate animals, as well as some other more primitive ones) Class Mammalia (includes all mammals)

What is limiting factor

Answers

Answer:

limiting factors are often referred as a lack of a particular resource .

Calculate the number of neutrons in atoms of the isotope 3919K
With working out

Answers

Answer:

20 neutrons

Explanation:

In the atom of an element, there exists three subatomic particles namely; proton, neutron and electron etc. The sum of the number of neutrons and number of protons (a.k.a atomic number) in an atom makes up the mass number.

In this question, an element was given as follows: 39- 19 K meaning that the mass number is 39 but the atomic number is 19.

Hence,

no of. neutrons = mass no. - atomic no.

39 - 19 = 20 neutrons.

Are lizards reptiles or amphibians?

Answers

Answer:

Reptiles are turtles, snakes, lizards, alligators and crocodiles. Unlike amphibians, reptiles breathe only through their lungs and have dry, scaly skin that prevents them from drying out

Amphibians are frogs, toads, newts and salamanders. Most amphibians have complex life cycles with time on land and in the water. Their skin must stay moist to absorb oxygen and therefore lacks scales. Reptiles are turtles, snakes, lizards, alligators and crocodiles.

Quadrupedal monkeys __________ Group of answer choices have long arched spines for flexible movement when leaping. hold their bodies parallel to the ground (pronograde posture) when walking have narrow rib cages with scapula on the side, limiting the range of motion in the shoulder All of these traits characterize quadrupedal monkeys

Answers

Answer:

All of these traits characterize quadrupedal monkeys

Explanation:

Quadrupedal monkeys have long arched spines that is used for flexible movement when jumping to a height. They hold their bodies parallel to the ground because of pronograde nature means these monkeys walking with the body by using four legs. While walking they have narrow rib cages with scapula on the side that limits the range of motion in the shoulder.

The islets of Langerhans are surrounded by

A-exocrine cells that produce digestive fluid.

B-endocrine cells that produce digestive fluid.

C- exocrine cells that produce glycogen

D-B and C

PLEASE HELP ME ITS REALLY URGENT CAUSE I HAVE EXAMS TOMORROW!!!

Answers

Answer:

A-exocrine cells that produce digestive fluid.

Explanation:

The pancreas is composed of both exocrine cells (capable of producing digestive enzymes) and endocrine cells (involved in the production of hormones that control glucose homeostasis). The islets of Langerhans are clusters or 'islands' of pancreatic endocrine cells, which consist of α-cells that produce glucagon, β-cells that secrete insulin and δ-cells that produce somatostatin. These islets are surrounded by: 1-exocrine cells called 'acini' that produce digestive enzymes such as chymotrypsin, trypsinogen, lipase, and elastase; and 2-bile ductules, which are tubes that carry bile from the liver and the gallbladder through the pancreas.

The most direct method by which an ion or molecule can move across a cell membrane is by ____________.A. using an integral protein.B. a sodium-potassium pump.C. simple diffusion. D. active transport.E. osmosis.

Answers

Answer:

C. Simple diffusion

Explanation:

Simple diffusion from a high to low concentration is the most direct method that ions or molecules can move across a cell membrane.

Through this method, no proteins are required for transport, and no energy is used.

The ion or molecule being transported directly moves across the cell membrane, without any other factors involved.

So, the correct answer is C. Simple diffusion

Which of the following is the best definition of the term nonrenewable
resource?
A. A resource that can be replaced naturally within a reasonable time
period
B. An inexpensive resource
C. An expensive resource
D. A resource that takes many years to replace

Which one A or D help me pls

Answers

Answer

D.

Explanation

Non renewable resources such as coal, if not used sustainably will get over fast and coal is used for many purposes, if this resource gets over it will take hundreds of years to come back

Answer:

D). A resource that takes many years to replace.

Explanation:

Trust me

What is the probability that a hybrid individual with the genotype AabbCcddEeFf will produce a gamete that has the genotype abCdeF

Answers

Answer:

6.25%

Explanation:

Find the number of different gametes that can be produced

number of gametes produced = 2^n

n is the number of allele pairs that are heterozygous

there are 4 heterozygous allele pairs, these are: Aa, Cc, Ee and Ff

2^4 = 16

abCdeF is one of the 16 genotypes that could be produced

to find the percentage, divide 1 and 16 and multiply by 100

1 ÷ 16 = 0.0625

0.0625 × 100 = 6.25

What is immigration?
A. When animals leave an ecosystem
B. When animals are born in an ecosystem
Ο Ο Ο O
C. When animals move into an ecosystem
D. When animals die in an ecosystem

Answers

Answer:

C. When animals move into an ecosystem

Explanation:

Porque las enzimas actuan sobre algunas sustancias ñ

Answers

Answer:

SPAM

Explanation:

SPAM

SPAM

What is the answer with explaining

Answers

Answer:

EeWw

Explanation:

The allele for the earlobe is depicted as e while that of the hairline is w.

A parent that is homzygous recessive for earlobes (ee) and homzygous dominant for hairline (WW) would have the genotype eeWW.

The other parent that is homzygous dominant for unattached earlobes and homzygous recessive for hairline would have the genotype EEww

eeWW    x    EEww

During meiosis, each genotype will form gametes.

eeWW: eW, eW, eW, eW

EEww: Ew, Ew, Ew, Ew

These gametes will randomly unite during fertilization to produce offspring. Since the same gametes are formed by each parent:

   eW   x   Ew

offspring

100% EeWw (see the attached image for the Punnet's square)

The genotype of all the children would be EeWw and would phenotypically be with unattached earlobes and dominant hairline.

a) explain covalent bond and hydrogen bond with an example of each (4marks)
b)state three(s) importance of surface tension and example( 6marks)​

Answers

Answer:

Covalent bond is a primary chemical bond formed by the sharing of electron pairs. Covalent bonds are strong bonds with greater bond energy. Hydrogen bond is a weak electrostatic attraction between the hydrogen and an electronegative atom

List three potential effects of errors occurring during meiosis.

Answers

Errors can occur during meiosis producing gametes with an extra or missing chromosome. The consequences of this following fertilisation depend on which chromosomes are affected. Often the embryo is not viable, but some of these errors can lead to trisomy conditions or sex chromosome disorders.

Explanation:

:)

Errors during meiosis can lead to mutations in gametes. Defective gametes that undergo fertilization may result in miscarriages or ultimately lead to genetic disorders.

Discuss who is impacted by desertification including which continents are impacted and the role of an increasing population.

Answers

Answer:

Africa and Asia.

Explanation:

Africa and Asia are the most affected regions in the world which is greatly affected by desertification. The climate change and human activities has a great impact in these areas which causes desertification as compared to other areas in the world. The increase in population increase the rate of depletion of natural resources which causes more pollution in the environment that leads to desertification in these areas.

Identify the molecule that is not a compound

Answers

Answer:

dear, your question seems to be incomplete.......plz ask it again

This could be Hydrogen gas as it is a molecule but is not a compound as it is only made up of one element!

heavenly bodies that travel around a planet are called satellites or moon which planet has no satellite or moon
A. mars and venus
B. jupiter and venus
C. jupiter and mars
D. mercury and venus​

Answers

Answer:

i think its A. i dunno if it is btw have a nice day

Answer:

D. Mercury and Venus

Our eyes are always the same size from birth​

Answers

Answer:

It's FALSE. Baby eyes are propotionallity larger than adult eyes.

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Explain the role of cellular respiration in a cell.

Answers

Answer:

Every cell performs a type of cellular respiration as it is required to produce energy. The point of cellular respiration is to turn high-energy molecules, know as glucose, into ATP, a form of usable energy. There are two types of respiration, aerobic and anaerobic. Aerobic goes through 3 stages and produces the most ATP by using oxygen and releasing carbon dioxide. Anaerobic does the same job but only uses one stage and produces much less ATP. This process gives the cell energy and continues the carbon cycle.

Describes vitamin D levels and problems

Answers

Answer:

Vitamin D is essential for strong bones,because it helps the body use calcium from the diet.

It’s important for normal growth and development of teeth and bones. It can help resist against certain diseases. Some problems if you take to much vitamin D is that you will get weakness, fatigue, sleepiness, headache, loss of appetite, dry mouth, metallic taste, nausea, vomiting, and others.
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