15. Assuming the fluorescence threshold is set to be 1. Which gene has the highest gene expression rate? What is the Ct value? Gene with highest expression = ______________ Ct value = _______________

Answers

Answer 1

Answer:

The correct answer is - Gene 1 and 14.5 (or 14).

Explanation:

The cycle threshold is denoted as Ct value. It is the cross point at the threshold line meets with fluorescence signal reaction curve. In the given RTpcr experiment, the threshold line is fluorescence intensity 1 thus, Ct value for each gene will be the point at which each curve meets the fluorescence intensity line at 1. Ct value of is inversely proportional to amount of nucleic acid in the given sample.

Here, the Ct for gene 1 is the lowest, so the amount of DNA would be highest.

Gene with highest expression= Gene 1

Ct value= 14.5


Related Questions

PLS HELP ASAP
ILL GIVE BRAINLY

Contrast the usefulness of absolute and relative dating techniques.

Answers

Answer:

In relative dating, fossils are dated according to the depth at which they were buried While in the absolute dating, isotopes of carbon are used for dating fossils. The absolute dating is more precise than relative dating because it tells the exact age of the fossils.

6. Compare the different measurements of the thermometer which are used.​

Answers

Having a reliable medical thermometer at home can be incredibly helpful. The ability to accurately find out if someone has a fever gives you much-needed information about important next steps for their care.

There are many types of contact and contact-free thermometers to choose from. The ages of your household members, as well as personal preference, can help you determine which types to buy.

Like any other device in your home, it’s important to understand how your thermometer works. Not every type works the same way or is designed to provide the same temperature readings.

Why can viruses not fit in any domain? How are they different from all other forms of Life? What would need to occur for viruses to be classified as "alive"?

Answers

Answer:

virus are obligate parasites. Viruses do not, however, carry out metabolic processes. Most notably, viruses differ from living organisms in that they cannot generate ATP.

When an outbreak of foot and mouth disease is discovered among livestock in a country, what is MOST likely the chosen way to control it?

Answers

Answer:

You can get infected with hand, foot, and mouth disease if you have the virus on your hands and then touch your eyes, nose, or mouth. To lessen your chance of getting sick, don't touch your eyes, nose, and mouth with unwashed hands.

You can get infected with hand, foot, and mouth disease if you have the virus on your hands and then touch your eyes, nose, or mouth. To lessen your chance of getting sick, don't touch your eyes, nose, and mouth with unwashed hands.

What are the mechanism that use to resist the antimicrobial agents?

There has been several mechanisms, like resistance to antimicrobial agents, encapsulation or secretation of proteins that affects and might destroy the phagocyte along with some leukocytes in our body perform phagocytosis as a defense mechanism against the pathogenic bacterium.

These phagocytes contain lysosomes, intracellular granules that possess bactericidal substances. When the phagocyte ingests the pathogen, a phagosome is formed, which merges with the lysosome, forming the phagolysosome, which is where toxic substances and enzymes kill the ingested microorganism.

There are several mechanisms by which a microorganism can survive this process as resistance to antimicrobial agents. Some bacteria use phenolic glycolipids from their cell wall, to eliminate toxic oxygen compounds.

Therefore, You can get infected with hand, foot, and mouth disease if you have the virus on your hands and then touch your eyes, nose, or mouth.

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Sequence three probable steps of fossil fish formation?

Answers

Answer:

鱼类和鱼形动物的区别主要在于口与鳃结构的基本不同

Explanation:

what gender of offspring are most often affected by sextinked inheritance?-What gender-are-usmally-carriers-of the trait?

-males, males
-males, females
-females, females
-females, males O O

Answers

Answer:

female, female

Explanation:

this is because sex-linked characters are normally borne in the X-chromosome

PLEASE HELP ME!!!! I am stuck.
The frequency of tasters and nontasters of PTC varies among populations. (Answer ALL questions)
- In population A, 94 percent of people are tasters (an autosomal dominant trait)
and 6 percent are nontasters.
- In population B, tasters are 75 percent and nontasters 25 percent.
- In population C, tasters are 91 percent and nontasters are 9 percent.

1. Calculate the frequency of the dominant (T) allele for PTC tasting in population A.
2. Calculate the frequency of the recessive (t) allele for nontasting in population A.
3. Calculate the frequency of the dominant (T) allele for PTC tasting in population B.
4. Calculate the frequency of the recessive (t) allele for nontasting in population B.
5. Calculate the frequency of the dominant (T) allele for PTC tasting in population C.
6. Calculate the frequency of the recessive (t) allele for nontasting in population C.
7. Assuming that Hardy-Weinberg conditions apply, determine the TT frequency in population A.
8. Assuming that Hardy-Weinberg conditions apply, determine the Tt frequency in population A.
9. Assuming that Hardy-Weinberg conditions apply, determine the tt frequency in population A.
10. Assuming that Hardy-Weinberg conditions apply, determine the TT frequency in population B.
11. Assuming that Hardy-Weinberg conditions apply, determine the Tt frequency in population B.
12. Assuming that Hardy-Weinberg conditions apply, determine the tt frequency in population B.
13. Assuming that Hardy-Weinberg conditions apply, determine the TT frequency in population C.
14. Assuming that Hardy-Weinberg conditions apply, determine the Tt frequency in population C.
15. Assuming that Hardy-Weinberg conditions apply, determine the tt frequency in population C.

Answers

Answer:

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fk.ydj.td.jtd.sjtd

ktdfylluflyfulgñugñugñugñuguñgpi

Explanation:

hhm y chupapico

Which is our body part help us to maintain balance.

Answers

Answer:

cerebellum

Explanation:

The cerebellum is a small part of the brain positioned at the back of the head, where it meets the spine, which acts as the body's movement and balance control centre.

please mark me as brainliest

Tai không chỉ có vai trò để lắng nghe mà còn hỗ trợ trong việc giữ cân bằng

A stem cell is
A. A specialized cell that can divide limitlessly but cant differentiate
B. A specialized cell that can divide limitlessly.
C. An unspecialized cell that cant divide limitlessly nor differentiate into specialized cell.

Answers

Answer:

B

Explanation:

They divide to form more cells called daughter cells under proper conditions

B

It is B because the stem cells divide and divide so its lile a pattern where it goes more and more cells

Hope this helps :))

Scenario 1:

Genetic engineering can be used to create more productive strains of farm animals used for milk and meat production. By adding genes to an animal’s DNA, the animal can be made to be more resistant to common infections. This can reduce the need to administer large doses of antibiotics to the animals.

Do you think that this type of genetic engineering should be pursued? Explain your answer. (5 points)

What are some possible impacts (positive and negative) of this type of genetic engineering on individuals, society, and the environment? (5 points)


Scenario 2:

Mike was adopted, and his biological family history is unknown. Although he is healthy, he would like some understanding of his genetic makeup, including potential health risks and genes that he could pass on to his children. Mike has heard about commercial laboratories that can compare segments of your DNA to those of people with common hereditary diseases in order to give you some idea of how susceptible you are to the diseases. The results of these types of tests are highly inconclusive. If Mike’s DNA showed that he shares similarities in a segment of DNA with people who have a given disease, his chances of developing that disease or passing it on to his children may be slightly elevated, but they are not 100 percent. These tests can cost more than $2,500 and are not covered by many insurance companies.

Do you think that Mike should undergo the genetic tests? Explain your answer. (2 points)

What are potential pros and cons of having such tests done? (5 points)

Describe how the availability of these genetic tests might affect the frequency of genetic diseases in individuals and populations. (3 points)

Answers

Due to brainly not accepting my essay, I have instead included a image of it. :D

A ______, such as a bean, has two seed leaves.

Answers

Answer:

A dicotyledonous plant

hope this helps you

what is uropoiesis?

Answers

Answer:

The production and excrection of urine

Answer:

The production and excretion or urine.

For each of the following structures, first indicate its function in the fetus; then, note its fate (what happens to it or what it is converted to after birth).

a. Umbilical artery
b. Umbilical vein
c. Ductus venosus
d. Ductus arterious
e. Foramen ovale

Answers

Answer:

1. Functions:

a. Umbilical artery >> carries deoxygenated blood from the fetus to the placenta

b. Umbilical vein >> transports oxygenated blood from the placenta to the fetus

c. Ductus venosus >> allows oxygenated blood from the placenta to bypass the liver

d. Ductus arterious >> allows most of the blood from the right ventricle to bypass the fetus's non-functioning lungs

e. Foramen ovale >> oxygenated blood from the umbilical vein to bypass the pulmonary circulation

2. After the bird:

1. Umbilical artery >> medial umbilical ligament

2. Umbilical vein >> round ligament of the liver

3. Ductus venosus  >> ligamentum venosum

4. Ductus arteriosus >> ligamentum arteriosum

5. Foramen ovale >> fossa ovalis

Explanation:

The umbilical artery is a paired artery localized in the abdominal and pelvic regions, which carries deoxygenated blood from the fetus to the placenta through the umbilical cord. The medial umbilical ligament is the obliterated part of the umbilical artery that arises from the internal iliac arteries. In utero, the umbilical arteries carry waste products back to the placenta, whereas the umbilical vein carries oxygenated blood from the placenta to the fetus. The round ligament of the liver (also known as ligamentum teres hepatis) is a remnant of the umbilical vein that  exists in the embryonic stage, it connects the left lobe of the liver to the umbilicus. The ductus venosus is a slender shunt that allows oxygenated blood from the placenta to bypass the liver, it connects the intra-hepatic portion of the umbilical vein to the inferior vena cava. The ligamentum venosum is an extrahepatic, slender, and fibrous remnant of the fetal ductus venosus that travels between the left portal vein and the inferior vena cava. The ductus arteriosus is a fetal artery that connects the aorta to the pulmonary artery. The ligamentum arteriosum is a nonfunctional vestige of the ductus arteriosus, it is attached to the superior surface of the pulmonary trunk. The foramen ovale is an oval-shaped, small, opening in the wall (septum) between the two upper chambers of the heart. The fossa ovalis is a vestige stricture of the foramen ovale of the embryonic heart, which forms a depression in the right atrium of the heart.

Which statement is not true about the field of science?
it is based on observations and explanations of the natural world.

Answers

You are right with your answer please

Actual Question:
Which statement about the purpose of the field of science is false? (1 point)

Science is used to investigate the natural world.

Events of the natural world can be explained by science.

Science works to establish a collection of unchanging truths.

Useful predictions come from derived explanations of science.
Answer:

Science works to establish a collection of unchanging truths.

Explanation:

I took the test

The second generation of purebred cross of -hom-ozygo us recessive organism, with a —hom-ozygous dominant organism, contain a ratio of approximately _______ expressed dominant traits to recessive traits.
A. 2:1
B. 1:1
C. 1:3
D. 3:1

Answers

Answer:

D. 3:1

Explanation:

Gregor Mendel, in one of his numerous studies, performed a cross between purebred dominant (TT) pea plants and purebred recessive (tt) pea plants. All the offsprings of this cross appeared heterozygous tall (Tt) in the F1 generation.

However, when he self-crossed the F1 generation i.e. Tt × Tt, Mendel obtained a phenotypic ratio of 3 tall plants (dominant traits) : 1 short plant (recessive). This showed that the masked gene (recessive) was still present.

1. Sensation is to as perception is to a. vision, olfaction b. conscious, unconscious c. awareness, interpretation d. taste, vision.​

Answers

Answer:

opt (c)

Explanation:

The large number of alveoli in lungs improve gas exchange by ________. increasing the surface area for diffusion decreasing the distance for diffusion changing the diffusion constant increasing the concentration difference

Answers

Answer:

Explanation:

The large number of alveoli in lungs improve gas exchange by . increasing the surface area for diffusion

Which is a compound that allows plants to get nitrogen from the nitrogen cycle?

Answers

Answer:

Plants can use ammonia as a nitrogen source. After ammonium fixation, the ammonia and ammonium that is formed will be transferred further, during the nitrification process. Aerobic bacteria use oxygen to convert these compounds.

In butterflies, having red antennas (denoted by “R”) is dominant to having white antennas (denoted by “r”). If both parents are heterozygous for red antennas, what are the predicted Phenotypic outcomes of their offspring? (hint: a punnett square will help you determine this).

In butterflies, having red antennas (denoted by “R”) is dominant to having white antennas (denoted by “r”). If both parents are heterozygous for red antennas, what are the predicted Genotypic outcomes of their offspring? (hint: a punnett square will help you determine this).

All the offspring of a cross between a black-eyed mendelien and an orange-eyed mendelien have black eyes. What is the expected F2 phenotypic ratio of a cross between two F1 black-eyed mendeliens?
A) 1 black-eyed:1 orange-eyed
B) 3 black-eyed:1 orange-eyed
C) 1 black-eyed:3 orange-eyed
D) 1 black-eyed:0 orange-eyed

Answers

The phenotypic ratio of the cross is as follows: 3 red antennas: 1 white antenna

For question 2, the phenotypic ratio of the F2 generation is: 3 black-eyed: 1 orange-eyed

QUESTION 1

According to the first question, a gene coding for antenna color in butterflies is involved. Red antennas (“R”) is dominant to white antennas (“r”). Hence, a butterfly with genotype "Rr" will be phenotypically red.

According to this question, If both parents are heterozygous for red antennas i.e. Rr (red antenna) × Rr (red antenna), the following gametes will be produced: R and r. Using these gametes in a punnet square (see attached image), the following phenotypic ratio of offspring is produced:

3 RED ANTENNAS (RR, Rr, Rr) : 1 WHITE ANTENNA (rr)

2. In the second question, all the offspring of a cross between a black-eyed mendelien (BB) and an orange-eyed mendelien (bb) have black eyes (Bb). In a cross between two F1 black-eyed mendeliens i.e. Bb × Bb, the following proportion of offspring is produced:

BB, Bb, Bb, bb

Hence, a phenotypic ratio of 3 black-eyed: 1 orange-eyed

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What is a major role that fungi play in ecosystems

Answers

Answer:

Fungi play a crucial role in the balance of ecosystems. They colonize most habitats on earth, preferring dark, moist conditions. They can thrive in seemingly-hostile environments, such as the tundra. However, most members of the Kingdom Fungi grow on the forest floor where the dark and damp environment is rich in decaying debris from plants and animals. In these environments, fungi play a major role as decomposers and recyclers, making it possible for members of the other kingdoms to be supplied with nutrients and to live.

Explanation:

Answer from Gauth math

Question 4 of 20
Which energy transfer occurs when a cube of ice is placed in a glass of
water?
A. The chemical energy of the ice is transferred to the liquid water.
B. The nuclear energy in the water molecules is released from the ice
into the liquid water.
C. Gravitational potential energy is transferred from the liquid water
to the ice.
O D. Thermal energy is transferred from the liquid water to the ice.
SUBMIT

Answers

Answer:

option (D) is the right answer

4. Which of the following are extra senses that vertebrates may have? (Choose all that apply)
magnetoreception
proprioception
baroception
o nociception

Answers

Answer:

- Proprioception

- Magnetoreception

- Nociception

The following are the extra senses that vertebrates may have:

Magnetoreception. Proprioception.Nociception.

Thus, the correct options are A, B, and D.

What is Vertebrate?

Vertebrates may be defined as those groups of animals that possess a backbone or spinal column. It includes mammals, birds, reptiles, amphibians, and fishes.

Magnetoreception is a sense that allows the organisms to detect the senses of the Earth's magnetic field and responds to it. Proprioception may be defined as a sort of sense that allows your body abilities to sense movement, action, and location.

Nociception may completely depend on the sensory nervous system that process in response to encoding noxious stimuli.

Therefore, the correct options for this question are A, B, and D.

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Porque crees que las personas llegan a consumir sustancias psicoactivas

Answers

Answer:

La causa exacta del consumo de drogas se desconoce. Los genes de una persona, la acción de las drogas, la presión de compañeros, el sufrimiento emocional, la ansiedad, la depresión y el estrés ambiental pueden ser todos factores intervinientes.

Explanation:

Put the following steps of Meiosis in the correct order:

I. The cell divides into 2 daughter cells
II. Homologous chromosomes pair up in the center of the cell
III. Spindle fibers separate homologous chromosomes
IV. Chromosomes become shorter and thicker
A. I, II, III, IV
B. IV, III, II, I
C. II, III, I, IV
D. IV, II, III, I

Answers

C.
Because first thing that happens in meiosis is the homologous pairs come in the center of the cell. Without that happening first, the cell can’t divide into 2 daughter cells, so it is NOT option A.
Chromosomes will become shorter and thicker at the end so the only option that remains is C.
Homologous chromosomes pair up in the center of the cell.Spindle fibers separate homologous chromosomes.The cell divides into 2 daughter cells.Chromosomes become shorter and thicker.

What is Meiosis

Meiosis  (listen); from Ancient Greek'lessening', since it is a reductional division) is a special type of cell division of germ cells in reproducing organisms that produce the gametes, such as sperm or egg cells. It involves two rounds of division that ultimately result in four cells with only one copy of each chromosome (haploid). Additionally, before the division, genetic material from the paternal and maternal copies of each chromosome is crossed over, creating new combinations of code on each chromosome. Later on, during fertilization, the haploid cells produced by meiosis between a male and female will fuse to create a cell with two copies of each chromosome again, the zygote.

Errors in meiosis resulting in aneuploidy (an abnormal number of chromosomes) are the leading known cause of miscarriage and the most frequent genetic cause of developmental disabilities.

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Devise an experiment to test the effect of pH on the activity of biological washing powder

Answers

Answer:

boil a water on a container deep your face inside it and you know the temperature ️ of water

This is the last

What are the vestigal organs present in human body??​

Answers

Answer:

Charles Darwin listed a number of putative human vestigial features, which he termed rudimentary, in The Descent of Man (1871). These included the muscles of the ear; wisdom teeth; the appendix; the tail bone; body hair; and the semilunar fold in the corner of the eye.

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Los microondas tienen una frecuencia mayor a las ondas de radio. verdadero o falso,justifique

Answers

Los microondas tienen una frecuencia mayor a las ondas de radio.Verdadero o Falso.

Verdadero.

→ Porque, los microondas con/y las radiofrecuencias son radiaciones electromagnéticas de mayor frecuencia.

SALUDOS!

MiraculousAlejandra

Macronutrients is the most readily available for energy production

Answers

Answer:

The correct answer is - true.

Explanation:

All the macronutrients carbohydrates, fats, and protein all three produce energy. Carbohydrates are the most preferred source of energy for the human body as it is the macronutrient that your system most requires.

The human body easily breaks down most carbohydrates and provides a significant amount of energy. The energy-providing process is called cellular respiration starts with glucose as a substrate. Glucose is the simplest carbohydrate.

Please help;
Given the range of current speeds shown on the graph above, would deposition ever be possible for clay (0.001 mm) or silt (0.01mm) particles?
Answer yes or no and explain your answer.

Answers

Answer:

Yes.

Explanation:

Yes, deposition can be possible for clay (0.001 mm) and silt (0.01mm) particles when the agent that carry its load is absent in that region such as wind. Wind is the agent that moves clay and silt particles with it so if there is high wind in a specific region, no deposition will occur while on the other hand, those areas where wind is not present, deposition will definitely occurs so we can say that deposition depends on the presence and absence of wind.

if you look away from this sentence and focus your eyes on a distant object, the eye muscles _______ to focus images on the retinas

Answers

If you look away from this sentence and focus your eyes on a distant object, the eye muscles relax to focus images on the retinas.

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