Answer:
Phenotypic approach for bacterial identification
Explanation:
Bacterial identification can be done by conventional methods, which are based on phenotypical characteristics. These methods are much affordable and reasonable.
Phenotypical identification is based on bacteria´s observable characteristics, such as their morphology, development, and biochemical/metabolic properties.
It is important to consider that these methods do not provide absolute certainty. They can only indicate the genera or species to which the bacteria under study may belong.
Some primary evidence is usually used for fast bacteria identification:
Gram staining, morphology, growth at different media or different incubation atmospheres, glucose fermentation, spores production, motion, aerobiosis/anaerobiosis, among others.Knowing that the bacteria in the exposed example was isolated and grown in culture, then Gram-stained and tested for biochemical reaction, we can assume that the approach for its identification is phenotypic.
What are three techniques that can be used to show that the electron transport chain is found on the matrix side of the inner mitochondrial membrane. Select all that apply.
Answer:
Explanation:
Step 1: Generating a Proton Motive Force
The hydrogen carriers (NADH and FADH2) are oxidised and release high energy electrons and protons
The electrons are transferred to the electron transport chain, which consists of several transmembrane carrier proteins
As electrons pass through the chain, they lose energy – which is used by the chain to pump protons (H+ ions) from the matrix
The accumulation of H+ ions within the intermembrane space creates an electrochemical gradient (or a proton motive force)
Step Two: ATP Synthesis via Chemiosmosis
The proton motive force will cause H+ ions to move down their electrochemical gradient and diffuse back into matrix
This diffusion of protons is called chemiosmosis and is facilitated by the transmembrane enzyme ATP synthase
As the H+ ions move through ATP synthase they trigger the molecular rotation of the enzyme, synthesising ATP
Step Three: Reduction of Oxygen
In order for the electron transport chain to continue functioning, the de-energised electrons must be removed
Oxygen acts as the final electron acceptor, removing the de-energised electrons to prevent the chain from becoming blocked
Oxygen also binds with free protons in the matrix to form water – removing matrix protons maintains the hydrogen gradient
In the absence of oxygen, hydrogen carriers cannot transfer energised electrons to the chain and ATP production is halted
Why do all proteins given a negative charge prior to electrophoresis?
Answer:
The principle for native gels when Coomassie is not added to the sample is that proteins are separated by a combination of size and charge. The charge in general depends on the number of amino acid residues that bear a positive or negative charge at the pH of the gel. So if running the gel at pH from 8.3 / 8.9, Asp and Glu will be negatively charged, Lys and Arg and His will be protonated and have a positive charge. The N- terminus would have a positive charge while the C terminus would have a negative charge. There might be exceptions depending upon the micro environment of each residue. Native gels can be run at acidic pH as well, to give another way of resolving proteins. Smaller proteins migrate faster than larger proteins. Also, quaternary structure is preserved, so a dimer will run as a dimer so the percentage of total acrylamide monomer used is usually lower than what is used for SDS-PAGE, e.g. 7.5% acrylamide
Which of the following is the best definition of anaerobic respiration?
Answer:
C.
Explanation:
Anaerobic respiration is a process of cellular respiration. In this type of cellular process of respiration, oxygen is not needed to break down complex food substances such as glucose. Energy is generated in the process which is used for other cell functions.
Therefore, the answer is C.
And here is another one, help meeeee HUHUHU, I'm exhausted (only for those who know the answer)
I need explanation for your answers, even though it's multiple choices, I still need your explanation for it.
DUE TOMORROW!
If your answer is NONSENSE it will be deleted as soon as possible!
But if your answer is CORRECT, HELPFUL, HAS AN EXPLANATION, I'll chose your answer as the BRAINLIEST ANSWER!
Answer:
14. (a) Pb
15. (d) Al
16. (A) increases
Explanation:
14.
In the reactivity series of metals an element can displace another element from a compound if and only if it is not placed below that metal, as the reactivity of metals decreases when moving from top to bottom- if a metal is placed below Hydrogen it means that it is less reactive than Hydrogen and hence cannot displace Hydrogen from its compound.
All options except A are placed below Hydrogen in the reactivity series, so they wont displace Hydrogen from its compound, but option (a) that is Pb can do so as it is above Hydrogen in the reactivity series.
15.
Al is placed below Mg in the reactivity series and hence cannot displace Mg from its compound.
16.
The reactivity of metals increases down the GROUP.
[CAUTION :- We were talking 'bout REACTIVITY SERIES in the top 2 questions where reactivity decreases down the group, but we're talkin' 'bout GROUPS of the periodic table here, where reactivity increases down the group.]
The size of metals increases down the group causing their valence electrons to drift far from the nucleus so that they can be lost easily. The metals have a property of losing electrons so the more electrons it can lose more will be its reactivity.
Hence, reactivity increases down the group.
the flowers of the ____ bloom with sunrise and close with sunset.
The Hardy-Weinberg principle is written as the equation p2 + 2pq + q2 = 1. What does prepresent?
Explanation:
Hardy-Weinberg principle can be illustrated mathematically with the equation: p2+2pq+q2 = 1, where 'p' and 'q' represent the frequencies of alleles. ... The principle behind it is that, in a population where certain conditions are met (see below), the frequency of the alleles in the gene pool will be constant.
