A DNA fragment is introduced into the lacZ gene of a plasmid, which also contains an ampicillin resistance gene. What is the appearance of bacteria transformed with this plasmid if they are spread on plates containing ampicillin and X-gal

Answers

Answer 1

Answer: White colonies that are resistant to ampicillin.

Explanation:

The lac operon is an operon required for the metabolism of lactose in enteric bacteria such as Escherichia coli . It has three structural genes, a promoter, an operator and a regulator, all regulated by the availability of glucose and lactose. The lac repressor, a protein, senses lactose and blocks transcription of this operon. It acts as a repressor when lactose is present. A catabolite-activating protein (CAP), on the other hand, acts as a glucose sensor. The bacterium should express the lac operon only when lactose is available and glucose is not available. Thus, genes can always be transcribed, except when the Lac repressor protein is bound to the operon region, for which it has a high affinity (i.e. in the absence of lactose), where the Lac repressor protein maintains its high affinity for the operon region, preventing RNA polymerase from transcribing the structural genes. Thus, the system remains closed with consequent energy savings for the bacterium. In the presence of lactose, it binds to the Lac repressor protein and generates a conformational change that decreases its affinity for the operator region. Thus, the operator region is left free, RNA polymerase can freely transcribe the structural genes and the synthesized β-galactosidase (an enzyme) can degrade lactose to glucose plus galactose for energy.  

Thus, the lac z gene encodes the enzyme β-galactosidase, which catalyzes the hydrolysis reaction of lactose to glucose and galactose. In gene cloning experiments, a compound called X-gal is used as an indicator of cells expressing the β-galactosidase enzyme. X-gal is hydrolyzed by the enzyme to galactose and another compound that is oxidized giving an insoluble blue compound. Thus, if X-gal and a β-galactosidase inducer are dissolved in the medium of a culture plate where the transformed bacteria is found, colonies grown on the plate that possess a functional lac z gene (either because they were not transformed by the plasmid, or if they were but the plasmid does not have the cloned fragment or gene that disrupts the lac z gene) can be clearly distinguished by their blue coloration. If they have another gene inserted interrupting the lac z gene, they will not be able to produce the enzyme that degrades X-gal, resulting in white colonies since X-gal is not degraded giving that characteristic blue color.

The white, non-transforming colonies are eliminated by adding an antibiotic to the medium for which the plasmid provides resistance (in this case ampicillin), so that we can select the recombinant colonies that carry the vector with our sequence, simply by their color.

So, if bacteria are transformed with a plasmid (with ampicillin resistance) cloned with a gene that interrupts the lac z gene, the bacteria will be white because they do not synthesize the enzyme that degrades X-gal and will be resistant to ampicillin.


Related Questions

1. Discuss at least two attributes of a study that you would look for to determine whether or not it is rigorous.

Answers

Some of the aspects that help you determine whether a study is rigorous are questions and hypotheses that can be answered, adequated method use, and bibliography.

Rigorous is a term that refers to a quality that focuses on accuracy and precision to comply with norms, precepts, and pre-established rules. This term is widely used in science to refer to the precision of a study or investigation.

Therefore, when it is established that a study is rigorous, its attributes must be taken into account, such as:

- Questions and hypotheses that can be answered: This aspect refers to the investigator having the ability to limit his study so that it is appropriate to do so and does not include too much information that makes his study "impossible".

- Adequated methods use: This refers to using the appropriate methods to reach feasible conclusions, for example, methods of observation, deduction, induction, among others.

- Bibliography: This refers to the research or study being supported by reliable sources so that it is as accurate as the information it provides.

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RNA thường bắt đầu bằng base nào

Answers

Answer:

Xin lỗi vì bản dịch xấu.  Nó được tạo thành từ các căn cứ adenine, cytosine, guanine và uracil.

Explanation:

So với RNA, DNA có thymine thay vì uracil trong một phân tử di truyền polymer.

How many newborn would you expect to have cystic fibrosis in a population of 10,000 people?

Answers

Answer:

6

Explanation:

10,000 people divided by 1700 is about 5.8

round 5.8 to a whole number at you will expect 6 people to have cystic fibrosis in a population of 10,000 people

What is the frequency of a wave that has a wavelength of .20 m and a speed of 22 m/s

Answers

Answer:

110 Hz

Explanation:

Find the frequency of the wave using the formula, frequency = speed / wavelength.

Plug in the speed and wavelength, and solve:

frequency = speed / wavelength

frequency = 22 / 0.2

frequency = 110

So, the frequency of the wave is 110 Hz

Explain why the lack of surfactant would result in respiratory distress. Furthermore, what type of acid-base disorder might she develop and how would her body compensate. Be detailed in your explanation and support your answer with facts from your textbook, research, and articles from scholarly journals.

Answers

Answer:

Due to lack of expansion of lungs.

Explanation:

The lack of surfactant compound would result in respiratory distress because Surfactant enables the lungs to expand more easily which allows easiness in respiration. Without surfactant, the air sacs in the lungs will collapse very easily. This collapse leads to decreased amounts of air or oxygen in the lungs which makes it very difficult for the people or infant to breathe.

describe why heart failure is so often referred as "congestive" heart failure ?

Answers

Answer:

Heart failure — sometimes known as congestive heart failure — occurs when the heart muscle doesn't pump blood as well as it should. When this happens, blood often backs up and fluid can build up in the lungs, causing shortness of breath.

Explanation:

MARK AS BRAINEST IF YOU LIKE MY ANSWER OR IF THIS ANSWER HELPS YOUITS TOTALLY UP TO U

100 POINTS!!!!!!
The theories surrounding the formation of our solar system are based on many

biased opinions
incorrect facts
non-testable data
scientific investigations

Answers

scientific investigations is the correct thing

Answer:

"scientific investigations."

Muscle cells that are worked often in exercise will require more mitochondria. If we use more mitochondria, it will require us to use more carbohydrates to build ATP’s. What if we run out of carbohydrates stored in our body? What macromolecule can we break down and use in cellular respiration?
Group of answer choices

A) sucrose

B) lactose

C) glycogen

D) lipids

Answers

Answer:

Lipids

Explanation:

M. magneticum can only survive in low-oxygen environments, which are typically found near the bottom of bodies of water.

a. True
b. False

Answers

b good luck on future quizzes !!

Which of the following statements best describes one difference between transcription and translation?

Answers

The answer is the first one

Partes del sistema linfatico

Answers

Answer:

Explanation:

Le parti principali del tessuto linfatico sono il midollo osseo, la milza, la ghiandola del timo, i linfonodi, i linfonodi e le tonsille.

PLEASE HELP ME!!!! I am stuck.
The frequency of tasters and nontasters of PTC varies among populations. (Answer ALL questions)
- In population A, 94 percent of people are tasters (an autosomal dominant trait)
and 6 percent are nontasters.
- In population B, tasters are 75 percent and nontasters 25 percent.
- In population C, tasters are 91 percent and nontasters are 9 percent.

1. Calculate the frequency of the dominant (T) allele for PTC tasting in population A.
2. Calculate the frequency of the recessive (t) allele for nontasting in population A.
3. Calculate the frequency of the dominant (T) allele for PTC tasting in population B.
4. Calculate the frequency of the recessive (t) allele for nontasting in population B.
5. Calculate the frequency of the dominant (T) allele for PTC tasting in population C.
6. Calculate the frequency of the recessive (t) allele for nontasting in population C.
7. Assuming that Hardy-Weinberg conditions apply, determine the TT frequency in population A.
8. Assuming that Hardy-Weinberg conditions apply, determine the Tt frequency in population A.
9. Assuming that Hardy-Weinberg conditions apply, determine the tt frequency in population A.
10. Assuming that Hardy-Weinberg conditions apply, determine the TT frequency in population B.
11. Assuming that Hardy-Weinberg conditions apply, determine the Tt frequency in population B.
12. Assuming that Hardy-Weinberg conditions apply, determine the tt frequency in population B.
13. Assuming that Hardy-Weinberg conditions apply, determine the TT frequency in population C.
14. Assuming that Hardy-Weinberg conditions apply, determine the Tt frequency in population C.
15. Assuming that Hardy-Weinberg conditions apply, determine the tt frequency in population C.

Answers

Answer:

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fk.ydj.td.jtd.sjtd

ktdfylluflyfulgñugñugñugñuguñgpi

Explanation:

hhm y chupapico

which of the specific classification is the specific organism found

Answers

Answer:

Species are as specific as you can get. It is the lowest and most strict level of classification of living things. The main criterion for an organism to be placed in a particular species is the ability to breed with other organisms of that same species.

Question 1 of 10
The picture below represents a nitrogen atom.
6
How many protons does this atom have?
Answer: 7

Answers

Answer:

where is the picture. I could understand your question

Question 12 of 25
How are photosynthesis and cellular respiration related?
A. The products of photosynthesis are the reactants of cellular
respiration.
O B. Cellular respiration is the process animals use to produce glucose,
while photosynthesis is the process plants use to produce
glucose.
C. Cellular respiration provides the energy for photosynthesis.
D. Photosynthesis produces water molecules, while cellular
respiration splits water molecules apart.
SUBMIT

Answers

Answer:

A) The products of photosynthesis are the reactants of cellular

respiration.

Explanation:

Photosynthesis converts carbon dioxide and water into oxygen and glucose, so that cellular respiration converts oxygen and glucose into water and carbon dioxide.

The largest population of a particular species that an ecosystem can support
is the ecosystem's
O A. biome
O B. biodiversity
O C. carrying capacity
O D. keystone species
VOU
O Type here to search
D
SA

Answers

The largest population of a particular species that an ecosystem can support is the ecosystem's carrying capacity.

What is a carrying capacity?

Carrying capacity in ecology refers to the number of individuals of a particular species that an environment can support.

Every ecosystem has a maximum number of biotic components that it can support to ensure the sustainability of its resources.

Therefore, the largest population of a particular species that an ecosystem can support is the ecosystem's carrying capacity.

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#SPJ1

Can somebody help me

Answers

Answer:

No they should not, heres why:

Explanation:

It has been proven on countless occasions that people forget details within minutes of an event. When you forget things, and you try to remeber, your brain will fill in the blanks with what it wants to see. In every case, if possible try to use hard evidence, ie: Videos, recordings, fingerprints, blood, and other stuff. If not that try to use multiple witness accounts and compare them.

ronmental Science
17.1.2 Quiz: Characteristics of Populations
Question 1 of 10
What do members of a population have in common? Select all that apply.
A. They live in the same area.
OB. They have the same density.
C. They are the same species.
D. They are the same size.
O E. They live at the same time.

Answers

Answer:

A,C,D

Explanation:

A- Makes Sense because if you were a member of a population then the other members live in the same area as you.

B- Doesnt make sense because even though you live in the same population as someone, it doesnt mean you'd have the same density.

C- Makes sense because the definition of population is "a group of individuals of the same species living and interbreeding within a given area.

D- Doesnt make sense everyone is different size.

E- Makes sense in order to be considered a member of a population you have to be living

Which of the following combinations of phylum and description is correct? (and why?)
a) Nematoda − roundworms, internal skeleton
b) Porifera − gastrovascular cavity, coelom
c) Echinodermata - radial symmetry as a larva, coelom
d) Platyhelminthes − flatworms, gastrovascular cavity, no body cavity

Answers

it’s answer c!! i just took that and i got it right

The phylum and description that is correct among the options is Platyhelminthes − flatworms, gastrovascular cavity, no body cavity

Flatworms belongs to the phylum platyhelminthes. They are said to have no true body cavity, but they do have bilateral symmetry. For the lack of a body cavity, it is called acoelomates.

They also have an incomplete digestive system. That is, the digestive tract has only one openings

From the above we can therefore say that Option d is the correct option that gives the true description of the phylum platyhelminthes

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Una de las aplicaciones más importa del electromagnetismo son

Answers

Answer:

Las principales aplicaciones del electromagnetismo se emplean en: La electricidad. El magnetismo. La conductividad eléctrica y superconductividad.

Explanation:

One way in which a virus gains control of a cell is to direct the synthesis of new viral proteins. Therefore, the synthesis of mRNA is a central issue for all viruses. How does a retrovirus like HIV that causes AIDS achieve this goal

Answers

The answer has to be retrograde

Life cycle of peziza

Answers

growth stage

While the pizza category was in the growth stage, the product life cycle of long-lived products was observed in the rice category.


reptilesimprovements over amphibians​

Answers

The person above me is correct

What is the role of pitutary gland in puberity

Answers

Answer:

Puberty has begun. The trigger for puberty in both boys and girls is the production of 'gonadotrophin releasing hormone' (GnRH) from a part of the brain called the hypothalamus. This hormone stimulates the pituitary gland to release two hormones, Follicle Stimulating Hormone (FSH) and Luteinizing Hormone (LH).

Explanation:

Please Mark me brainliest

Answer:

the gland produce hormones that help to regulate growth and body functions.

Under normal conditions the sympathetic division is usually active in the body resting heart rates are influenced by nor epinephrine the parasympathetic system keeps heart rate lower than the heart would beat on its own. all of the above are true

Answers

Answer:

the parasympathetic system keeps heart rate lower than the heart would beat on its own.  

Explanation:

The parasympathetic nervous system (PNS) is one division of the autonomic nervous system. The vagus (parasympathetic) nerves that innervate the heart release acetylcholine in order to slow the heart rate by decreasing the slope of the pacemaker potential. Acetylcholine activates a type of G-coupled protein receptor known as muscarinic receptors, allowing for a parasympathetic reaction in the cardiovascular system (where these receptors are expressed), and thereby decreasing cardiac output by inhibiting heart rate. During rest, PNS predominates and controls the heart rate at a resting rate of 65-80 beats/minute (bpm), if parasympathetic nerves to the heart are cut, then the heart beats at its own rate of about 100 bpm.

What's is biosynthesis

Answers

The answer is:

Biosynthesis is a process in which substrates are converted to more complex products with inf living organisms or cells.

In biosynthesis, simple compounds are modified, converted into other compounds, or joined together to form macromolecules. This process often consists of metabolic pathways.

Answer:

the part of an organism close to the flesh and the cells

Explanation:

Biosynthesis is a multi-step, enzyme-catalyzed process where substrates are converted into more complex products in living organisms. In biosynthesis, simple compounds are modified, converted into other compounds, or joined together to form micro molecules. This process often consists of metabolic pathways.

What is the best way to ensure that results of a study are generalizable to a population?

By making sure to use as many participants as possible

By making sure the results are significant

By making sure to use an equal number of men and women

By using random assignment

By using a random selection of people in that population

Answers

The best way to ensure that results of a study are generalizable to a population is by using a random selection of people in that population

The goal of every research experiment is to obtain results that are  generalizable to a whole population.

This is achieved by the method of random selection. In random selection, members of the of the population are selected in such a way that is representative of the entire population. If for instance, I want to study a school having six classes which comprises of boys and girls. There are 700 students in the school and I need only 180 students to participate in a study whose results will be extrapolated to all the 700 students. I can decide to randomly select 15 boys and 15 girls from each class to participate in the study. This is an example of random selection.

In this way, the results obtained from the study is reflective of the whole population even though only a sample of the population actually participated in the study.

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What determines the temperature of a layer of the atmosphere?
A. The way thlmayer interacts with the layer below it
B. The average wind speed in the layer
c. The way the layer interacts with solar radiation
D. The altitude of the solar radiation

Answers

The answer is C. The way the layer interacts with solar radiation.

Answer: c

Explanation:

Correct just did it

Cells dispose of large waste molecules through a process called

osmosis.
diffusion.
exocytosis.
endocytosis.

Answers

Cells dispose of large waste molecules through a process called exocytosis.

Exocytosis is the process through which cells can eliminate wastes, which might be useless or toxic, to the extracellular space.

-------------------------------

The endocytosis and exocytosis process refers to molecules transport mediated by vesicles.

Exocytosis occurs from and into the cell and needs energy to happen because it is active transport.Exocytosis involves vesicles or vacuoles. Vesicles or vacuoles can carry substances destined for exportation or cellular wastes. Wastes may be large molecules that result useless for the cells or that might be toxic. During the exocytosis process

         1) excretion compounds of varying nature accumulate within the  

             vesicle

         2) The vesicle migrates toward the cellular surface.

         3) Once in the surface, the vesicle's membrane fuses with the cellular

             membrane, and its content is expelled from the cell to the  

             extracellular space.

         4) Vesicle membrane remains fused to the cell membrane until it is

              reused during endocytosis events.

------------------------------

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What is
FOOD & NUTRITON 1​

Answers

Answer:

1 How food keeps us healthy. Food is needed for energy and nutrients to exist, it provides energy for work, and warmth for the body. Everybody also needs food to build, maintain and repair their body. It is also required for control of body processes and for protection against disease and infections.

Answer:

Any nutritious substance that people or animals eat or drink or that plants absorb in order to maintain life and growth.

Nutrition is the biochemical and physiological process by which an organism uses food to support its life.

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