An ampicillin-resistant, tetracycline-resistant plasmid, pBR322, is cleaved with PstI, which cleaves within the ampicillin resistance gene. The cut plasmid is ligated with PstI digested Drosophila DNA to prepare a genomic library, and the mixture is used to transform E. coli K12. Which Antibiotic should be added to the medium to select cells that have incorporated a plasmid

Answers

Answer 1

Answer:

tetracycline

Explanation:

A plasmid is a small, circular, double-stranded DNA molecule, which is physically separated from chromosomal DNA and is able to replicate independently. Moreover, plasmid transformation is the process by which exogenous DNA is introduced into a cell (usually a bacterial host cell). In this case, Escherichia coli K12 cells were transformed with the pBR322 plasmids that encode both ampicillin and tetracycline resistance genes. According to data, plasmids were firstly cleaved with the PstI type II restriction endonuclease within the ampicillin resistance gene, thereby these plasmids lose the ampicillin-resistance gene that allows selecting E. coli K12 cells by their resistance to ampicillin. In consequence, tetracycline is the only one selective marker that will indicate that the bacteria have incorporated the plasmid.


Related Questions

Write two functions of neurons.​

Answers

Answer:

hope it helps you

Explanation:

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↦Any 2 functions of the neuron can be :-

They conduct electrical impulses thus helping to transmit messages to the brain, glands, muscles or other nerve cells.They are responsible for receiving sensory input from the external world, for sending motor commands to our muscles, and for transforming and relaying the electrical signals at every step in between.

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What is the function of nervous tissue for transport within an organism?
a) To dilate your blood vessels so that you will faint
b) To construct your blood vessels so that you will pass out.
c) To send signals to the blood vessels to constrict or dilate, to decrease or increase blood flow, respectively.
d) To send signals to the guard cells to swell and close the stomata or constrict to open up the stomata​

Answers

Answer:

C

Explanation:

To send signals to the blood vessels to constrict or dilate , to increase or decrease blood flow respectively.

la unica fuente de los aminoacidos esenciales son los alimentos y su anotemcion requiere de un gran gasto emergetico.si uma persona no consume estos aminoacidos se vera afectado el paso​

Answers

Answer:

Los aminoácidos son compuestos orgánicos que se combinan para formar proteínas. Los aminoácidos y las proteínas son los pilares fundamentales de la vida.

Cuando las proteínas se digieren o se descomponen, los aminoácidos se acaban. El cuerpo humano utiliza aminoácidos para producir proteínas con el fin de ayudar al cuerpo a:

Descomponer los alimentos

Crecer

Reparar tejidos corporales

Llevar a cabo muchas otras funciones corporales

El cuerpo también puede usar los aminoácidos como una fuente de energía.

list two importance of gravitational force?​

Answers

Explanation:

i .The gravitational force of the earth keeps us bound to the earth.

ii .Gravitational force between earth and sun makes the earth move around the sun. G

Answer:

1. gravity holds the planets in orbit around the sun and keeps the moon in orbit around the earth.

2. moons gravitational force to earth pulls the ocean tides, without that the land on earth would sink due to tsunamis and no control over the currents.

5.Can you write which kind of food items are not digested easily when gall bladder of a person is removed surgically? Why?​

Answers

Answer:

hi

Explanation:

no answer please try let us

Answer:

hard or solid ofood items are not digested easily when gall bladder of a person is removed surgically.it is because gall bladder is responsible to make a food in a small particle.

Fruit trees in a forest are an important habitat for a bird species and were historically a major source of food for a rodent species. Over time, the rodent species shifted their feeding behavior from fruit trees to nut trees. As rodent behavior changed, the density of fruit trees decreased, which caused significant decreases to the bird population. What kind of effect did the change in rodents exert on bird population size?

Answers

Answer:

The bird population might have to migrate in search for food or may decline in numbers.

Explanation:

This is a concept of primary producers that is trees and secondary consumers that is mouse and bids. Due to the decline in raw material available the mouse population declines and this also affects the secondary consumers that are birds of prey like hawks, owls etc.

Which level of organization includes all the other levels of organization? organ system organism tissue
cell

Answers

Answer:

organ system level of organization

cell>tissue >organ >organ system >multicellular organism

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What is one difference between primary and secondary succession? Primary succession can start from bare rock; secondary succession starts from organic soils. Primary succession occurs in habitats with rich organic soil; secondary succession does not. Primary succession is always caused by human disturbances; secondary succession is not. Trees are often the first colonizing species in primary succession, but trees are never the first species in secondary succession.

Answers

Answer:

Primary succession can start from bare rock; secondary succession starts from organic soils.

Explanation:

Primary succession is when the growth of pioneer species rake place in an bare and new environment. The secondary succession is when the growth of species take place due to the disturbances in the primary succession. The secondary succession is based on fire, floods, or any man-made disturbances in the region.

How will the concentration of H+ and OH- ions change when a substance with a pH of 3.2 is added to water?

Both H+ and OH- will increase
Both H+ and OH- will decrease
H+ will increase and OH- will decrease
H+ will decrease and OH- will increase

Answers

Answer:

H+ will decrease and OH- will increase

which of the following is a double layer of phospholipds with proteins

Answers

There aren’t any options but the answer is: cell membrane.
A cell membrane is composed of a phospholipid bilayer. The head is hydrophilic (water-loving), while the tail is hydrophobic (repels water).

What are the reactants in the following chemical reaction?
C6H12O6 + 6 O2 → 6 CO2 + 6 H2O
A. Carbon dioxide (CO2) and water (H2O)
B. Carbon dioxide (CO2) and oxygen gas (O2)
C. Glucose (C6H12O6) and oxygen gas (O2)
D. Glucose (C6H12O6) and water (H2O)

Answers

Answer:

its (A)

Explanation:

These reactions, which are described in detail in the chapter Cells and Energy, break down the simple sugar glucose (C6H12O6). The process uses oxygen and glucose and results in carbon dioxide (CO2), water (H2O), and usable energy. Oxygen and glucose are the reactants.

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I can't understand this sentence structure, so if somebody could please help me and have the right answers I will give brainliest.

Answers

i think it is
research - a
conclusion - b
analysis - c
experiment - d

Magnetic fields on the sun are produced by the sun’s uneven rotation and:
A. The movement of sunspots on the sun’s surface.
B. The movement of gases in the corona.
C. Gases in the convective zone.
D. Gases in the radiation zone.

Answers

Answer:

The correct answer is - C. Gases in the convective zone.

Explanation:

The heat of the sun causes the atoms to be ionized and create a charged gas known as plasma. These gases or plasma are move in the convective zone with the uneven and rapid rotation of the sun.

This creates the electric currents due to the charge in gases which ultimately create magnetic fields. Sun's rotation causes the sun's overall magnetic field to have some degree of order and to be somewhat stable over time.

please help...
in most stems,the epidermis has pores called..........​

Answers

Answer:

do you mean Stomata?

those pores are called stomata

Answer:

stomata

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Summarize the mRNA translation process in eukaryotes.

Answers

Answer:

During protein synthesis, the sequence of a messenger RNA (mRNA) molecule is translated into a sequence of amino acids. After the transcription of DNA to RNA, ribosomes in the cytoplasm or the endoplasmic reticulum (ER) create proteins.

OAmalOHopeO

How many kingdoms are there in the domain Bacteria?
O A. 4
B. 3
C. 2
O D. 1
SUBMI

Answers

Answer:

One kingdom. Domain bacteria has one kingdom, kingdom eubacteria

Explanation:

This is the answer I hope this helps you out!

What conditions need to be present for an atom to form hydrogen bonds?

Answers

fluorine, oxygen, or nitrogen. These three elements are so electronegative that they withdraw the majority of the electron density in the covalent bond with hydrogen, leaving the H atom very electron-deficient.

For an atom to form hydrogen bonds, it needs to be bonded to a highly electronegative atom (such as nitrogen, oxygen, or fluorine) and have a hydrogen atom attached to it.

Hydrogen bonds are intermolecular forces that occur between a hydrogen atom bonded to a highly electronegative atom and another electronegative atom nearby. The highly electronegative atom, often nitrogen (N), oxygen (O), or fluorine (F), attracts the shared electrons in the covalent bond towards itself, creating a partial negative charge. This leaves the hydrogen atom with a partial positive charge.

The conditions necessary for hydrogen bonding to occur are:

Presence of a Hydrogen Atom: There must be a hydrogen atom (H) bonded to an electronegative atom (N, O, or F) in the molecule or adjacent molecules.Highly Electronegative Atom: The electronegative atom (N, O, or F) must have a strong attraction for electrons, creating a significant partial negative charge.Close Proximity: The electronegative atom with the partial negative charge must be close enough to another electronegative atom in another molecule or nearby region to allow for electrostatic attraction.

These conditions are essential for the formation of hydrogen bonds. The hydrogen bond itself is a relatively weak force compared to covalent or ionic bonds, but it is significant in biological systems and plays a crucial role in many biological processes. Hydrogen bonding contributes to the unique properties of water, the stability of protein structures, the binding of DNA strands, and various other molecular interactions.

In summary, for an atom to form hydrogen bonds, it must be bonded to a highly electronegative atom (N, O, or F) and have a hydrogen atom attached to it, with another electronegative atom in close proximity to allow for the formation of electrostatic interactions.

To learn more about hydrogen bonds, here

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which of the folowing best decribes the shape of a DNA moleclue?

Answers

Answer:

The answer is letter A, "Two strands of nucleotides bonded together at their bases, twisting to form a double helix." When it comes to RNA, a single helix will form.

1. a) Name a set of homologous structures.

b) Two species have homologous structures. What do these homologous structures show about the evolutionary relationship between the two species?

Answers

the forearms of vertebrae

homologous structures mean that the two species have a common ancestor

what is photosynthesis​

Answers

Answer:

Photosynthesis is where the plants take in food through the cells! (such as light and carbon dioxide.)

Explanation: The process by which green plants and some other organisms use sunlight to synthesize foods from carbon dioxide and water. Photosynthesis in plants generally involves the green pigment chlorophyll and generates oxygen as a byproduct.

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The chemical energy stored in ATP during photosynthesis is released during the dark phase to

a) produce O2
b) produce CO 2
c) release energy for the "light"
d) produce a carbohydrate from CO 2

Answers

Answer:

A) produce O2

Explanation:

This is the exchange cycle between the plants and animals. Animals give off carbon dioxide (CO2) as an excess waste while the plants give off oxygen (O2).

Produce O2 took the assignment and got it right :)

What factor is described as sexual
selection?
A, non-random mating
B. migration
C. small populations
D. natural selection

Answers

Answer:

The correct answer is - D. natural selection.

Explanation:

Sexual selection takes place due to competition between individuals of the intraspecies same-sex and of mate choice which driving the evolution of reproductive traits.

Sexual selection is a mode of natural selection as it leads to the evolution of specific traits. Other examples or options are not related to sexual selection directly and are not affected by sexual selection.

PLS HELP -
Drag the tiles to the correct boxes to complete the pairs.
Match the pollution control method to its description

Answers

Answer:

Incineration: Solid pollutants are burned...    

Settling: Polluted water is aloud to stand still...

Composting: Microbes are used to convert...

Gas adsorption: Pollutants are captured through contact with hard...

Explanation:

Amazing nowone else got to your question. Your welcome.

please answer this:)) :((​

Answers

Answer:

Winter in the Northern Hemisphere begins December 21 or 22, when the North Pole is tilted a full 23.5° away from the sun. This day is known as the winter solstice. In June, in the northern hemisphere summer, the Sun's rays — and warmth — reach all the way to the north pole.

In June, in the northern hemisphere summer, the Sun's rays — and warmth — reach all the way to the north pole. In December, in the northern hemisphere winter, the north pole is tilted away from the incoming sunshine.

Explanation:

hope this helps u

Which of the following correctly describes a way in which Earth’s atmosphere interacts with the geosphere?

Answers

Answer:

It provides a medium for cycles that provide nutrients to living organisms.

3) Write down the names of organs that participate in respiration I explain any two of of them.​

Answers

Here you go now you can mark brainliest for the person above me

how does the presence of an acid in a water solution affect the pH of the solution

Answers

Explanation: When a solution has pH less than 7 then it is known as an acidic solution. In an acidic solution there are more number of hydrogen ions than hydroxide ions. ... Hence, we can conclude that the presence of an acid in a water solution affect the pH of the solution as it raises the pH.

Question 10 of 10
Which best describes the flow of genetic information?
A. Genetic information is carried only by DNA.
B. Genetic information flows from DNA to RNA to protein.
C. Genetic information flows from gene to allele to protein.
D. Genetic information cannot be transmitted outside the nucleus.

Answers

Answer:

B. Genetic information flows from DNA to RNA to protein.

Explanation:

The central dogma of biology describes the flow of genetic information within a biological system. This dogma is often stated as "DNA makes RNA, and RNA makes protein."

What are sporophytes and gametophytes?

Answers

Answer:

A. Two different forms a plant can have

Explanation:

Higher plants, lower plants (mosses) and ferns undergo a life cycle called ALTERNATION OF GENERATIONS. In this life cycle, they switch between a haplontic and diplontic forms of life.

The haplontic (haploid) life form which produces haploid (n) gametes is called GAMETOPHYTE while the diplontic (diploid) life form which produces the spores that germinates is called SPOROPHYTE. Hence, sporophyte and gametophyte are two different forms a plant can have.

what is the difference between artificial kidney and natural kidneys?​

Answers

IMPORTANCE The natural kidney continuously receives the blood and water, filter them and keep the body away from toxic wastes by the process of excretion. The artificial kidney is the device used in kidney damage persons only from time to time, under supervision of a specialist doctor.
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Arya has a homeowners policy that covers up to $400,000. Her house is completely destroyed in a fire. When an assessment is made of the house and possessions, it is decided that her losses are only worth about $350,000. How much money will Arya receive from her insurer (assuming there is no deductible)? A. $50,000 B. $200,000 C. $350,000 D. $400,000 Find the area of athletic field if it's length is 120cm and its width is 28cm .A. 397.6cm B. 3360cm C. 296 cm D. 4592cm E. 3356cm Identify the parts of speech of the following sentence-Until today, I never knew that some fish could walk on land what is the- subject, verb, adjective, adverb, direct object, preposition,object of the preposition, prepositional phraseplease help!! Literally what is this lol Which inscription describes the functions of local self-government during the Chola period? Which option will provide the best outcome when saying no to harmful substances? Leave the location. Ignore the person. Limit the amount. Ask for more time. CHECK ALL THAT APPLY! Why did slaveholders support settlement of freed slaves in Africa?O A. They wanted to keep freemen away from slaves.O B. They believed it would result in a better life for freemen.O c. They gained wealth from the transport of freemen.D. They felt freemen were taking jobs from whites.SUBMIT Select the correct answer.Which is the minimum or maximum value of the given function?dndnsn Place the words in their correct order. Alpha Company manufactures Product P and sells it in packs of 10 units. The actual results for the first week in January are as follows: Actual production 25,000 packs of Product P Actual cost of raw material Q 95,500 units at $0.85 Actual cost of labor 500 hours at $6.25 Standard quantity of raw material 96,000 units for 25,000 units of Product P Standard price of raw material Q $0.77 Calculate the total material variance for Product P for the first week in January ________invention made it possible for teenagers to have a way to communicate with their friendsover long distances.* samuel morses * thomas edisons * alexander graham bells Anne a ____________ robes ______ son amie. A. moins, de B. autant de, que C. aussi, que D. plus, que Cul es su punto de vista sobre llevar mascarilla para todos lados? A uniform plastic block floats in water with 50.0 % of its volume above the surface of the water. The block is placed in a second liquid and floats with 23.0 % of its volume above the surface of the liquid.What is the density of the second liquid?Express your answer with the appropriate units. Write a program to input value of three sides, to check triangle is triangle is possible to form of not The only way to decrease your lifestyle budget is by completely eliminating an item.TrueFalse Vit khai trin theo cng thc nh thc Niu-tn (x + 1) ^ 5 A ball is dropped from a height of 9 feet and allowed to bounce. Each time the ball bounces, it reaches a height that is23the height of the previous bounce. How many times will the ball be exactly 3 feet above the ground? One circle has a circumference of 12 pie Another circle has a circumference of 32 pie What is the ratio of the radius of the smaller circle to the radius of the larger circle? what is the good that you have selected?