"CRISPR" stands for Clustered Regularly Interspaced Short Palindromic Repeats, which are the hallmark of a bacterial defense system that forms the basis for CRISPR-Cas9 genome editing technology. In the field of genome engineering, the term "CRISPR" or "CRISPR-Cas9" is often used loosely to refer to the various CRISPR-Cas9 and -CPF1, (and other) systems that can be programmed to target specific stretches of genetic code and to edit DNA at precise locations, as well as for other purposes, such as for new diagnostic tools. How can this tool be used to alter genes in various organisms?

Answers

Answer 1

Answer:

by designing short guide RNAs (sgRNAs) customized to target genes of interest in the cells of these species

Explanation:

The CRISPR-Cas9 editing system is a versatile and powerful genome engineering tool for editing genomes, which can be directed to alter almost any DNA sequence in order to modify gene function. This system consists of an endonuclease protein (Cas 9) that cuts DNA at specific sites guided by a short guide RNA (sgRNA), which binds by base complementarity to the target sequence. This sgRNA must be designed with efficiency and specificity to target genes of interest. In consequence, the CRISPR-Cas9 genome editing system produces DNA double-strand breaks which may be repaired by 1- error-prone nonhomologous end joining (NHEJ) or 2-homology-directed repair (HDR) DNA repair pathways. According to the DNA repair pathway that has been activated, it is possible to trigger genetic modifications in the cells of different species (i.e., plant cells, animal cells, human cells, etc).


Related Questions

describe one piece of evidence represented by the contour line on the map that indicates the north side of chimney bluffs is steep?

Answers

Incomplete question. Attached is the image of the map below.

Explanation:

From the map, we could notice the contour lines around the Chimney Bluff areas are closely packed together. In other words, the spacing between the contour lines is narrower than those found in other locations.

This assertion is accurate because it is a standard practice in many maps to indicate the steepness of an area by having the contour lines in close proximity.

Which refers to the sum of all the forces that act upon an object?

A. net force

B. absolute force

C. balanced force

D. positive force

Answers

Answer:

Net force

Explanation:

Net force is the vector sum of forces acting on a particle or body. The net force is a single force that replaces the effect of the original forces on the particle's motion. It gives the particle the same acceleration as all those actual forces together as described by the Newton's second law of motion.

Answer:

net force

Explanation:

A hiker has become overwhelmed with heat while walking in the Grand Canyon. The hiker's body goes into overdrive to keep cool in the heat. The hiker needs to hydrate his or her cells. What process is used to regulate homeostasis?

A. Balanced equilibrium
B. Passive transport
C. Cellular transport
D. Hydration

Answers

I think D is the answer

BIOLOGY, I need help on this one

Answers

This is a base deletion

Momentum explains how _____ travels.
A.) sound
B.) heat
C.) both of these
D.) none of these

Answers

Answer:

I think D

Explanation:

I think it’s none. I’m not very confident tho. But momentum wouldn’t rlly relate to sound and heat.

a cell is placed in a isotonic solution. How does the cell maintain homeostasis in the environment ?

Answers

Answer:

If a cell is placed in an isotonic solution, there will be no net flow of water into or out of the cell, and the cell's volume will remain stable. If the solute concentration outside the cell is the same as inside the cell, and the solutes cannot cross the membrane, then that solution is isotonic to the cell.

Explanation:

HELP ME PLZZ I REALLY NEED HELP WITH THIS AND PLZZ EXPLAIN HOW YOU GOT YOU ANSWER WHEN YOU ARE DONE!!

Answers

Answer:

B.

Explanation:

The organ system is composed of multiple organs that work together to carry out a function.

There are some similarities between prokaryotic and eukaryotic cells. Which of the following structures is found in both prokaryotic and eukaryotic cells?

Answers

Answer:

Plasma membrane, cytoplasm, ribosomes, and DNA.

Explanation:

There are what eukaryotes and prokaryotes have in common.


30) These two equations are related because the BLANK of cellular respiration are the BLANK of photosynthesis. The BLANK of photosynthesis are the BLANK of cellular respiration.
Plz fill the blanks in :(

Answers

Answer:

Explanation:These reactions occur in the stroma the fluid filled area of a chloroplast ... Photosynthesis Respiration amp Interdependence Two of the 5 basic habitat resources are food and air. ... Without oxygen a cell can extract a net gain of only _____ molecules of ATP from ... Fill in the blanks in the Photosynthesis equation below 15.

In the two step cellular process of transcription and translation
(A) chromosomes line up and then are pulled to opposite ends of the cell.
(B) stem cells develop in embryonic cells, which then form adult tissue cells.
(C) the DNA double helix is separated and then replicated to form two strands of DNA.
(D) a DNA sequence is copied to form an RNA sequence, and then copied to form a protein.

Answers

Answer:

The answer is D

A is cell division, B is some stage of pregnancy, and C is DNA replication.

34. Education, Fuel, Food and Clean water are all listed as important factors in limiting….
a. Human Development index
b. Gross Domestic Product
c. Infant Mortality
d. Carrying Capacity

Answers

Answer:

A. Human Development index

Explanation:

The Human Development Index is a statistic composite index of life expectancy, education, and per capita income indicators, which are used to rank countries into four tiers of human development.

hope i helped

its not gdp nor infant mortality nor carrying capacity

and much more

Answer: the answer is A

Explanation:

Add the following complex numbers:
(6-2i)+(11+6i)
A. 17 + 4i
B. -5 + 8i
C. -5 +4i
D. 17 + 8i


HELP PLEASE

Answers

Answer:

A. 17 + 4i

Explanation:

Explain how diffusion and osmosis are related to the symptoms of cystic fibrosis

Answers

Answer:

Answer D

Explanation:

just took the test.

The destruction of which transport vessel in the plant results in a faster death??​

Answers

Answer:

All of the following are plant adaptations to life on land except ... D) The genes for the synthesis of transport proteins were destroyed. ... A) Xylem tracheids and vessels fulfill their vital function only after their death. ... 51) Ignoring all other factors, what kind of day would result in the fastest delivery of water and minerals to ...

Explanation: MARK BRAINLIEST

Before cells divide, they must replicate their entire genome. Explain why
replication of the genome prior to cell division is important.

Answers

Each time a cell divides into two daughter cells, its full genome is duplicated; for humans and other complex organisms, this duplication occurs in the nucleus. This minimizes the incidence of errors (mutations) that may greatly affect the resulting organism or its offspring. ...

HOPE THIS HELPED <3

Answer:

In order for all of the cells in your body to maintain a full genome, each cell must replicate its DNA before it divides so that a full genome can be allotted to each of its offspring cells. If DNA replication did not take place fully, or at all, the offspring cells would be missing some or all of the genome.

Explanation:

Please help me please

Answers

Answer c
Because the lowest one is at the bottom near 5 and the top one is on 25m and for it to be at 11m will be the wrong answer.
THE CORRECT ANSWER IS C IT MAKES SENSE

17. Match the major types of cancer with the types of cells they occur in
Adenocarcinomas
a. The lowest layer of the epidermis (skin)
Basal cell carcinoma
b. upper layers of skin, the lining of stomach, lungs,
kidneys
Squamous cell carcinoma
c. melanin-producing cells of the skin
Sarcoma
d. immune cells in the blood (T cells, B cells, and
bone marrow)
Leukemia and lymphoma
e. bones, fat, blood vessels, tendons, ligaments
Multiple myeloma
1. cells that produce fluids (breast, colon)
Melanoma
g. plasma cells (a type of immune cells)​

Answers

LolsbdgsbcgcsnnsjzkmcjMCk

Diffusion takes place

Answers

Answer:

yes

Explanation:

brainliest ???

What are the 3 main functions of this organ system?

Answers

Answer: Circulatory system, Cardiovascular system, and Digestive system.

Explanation:

when benedicts solution is added to a sucrose solution and put into a boiling water-bath , no change in color is observed

state why no color change is observed

Answers

Answer:

b

Explanation:

Mt. Pinatubo, a volcano in the Philippines, erupted in 1991. The eruption resulted in the cooling of Earth's surface
for two years.
What can you deduce from the information given?
O Solar energy seeped through the atmosphere,
O The eruption caused sunspot activity to increase.
O The volcano released a lot of sulfur dioxide and ash,
O Greenhouse gases caused the cooling of Earth's surface.

Answers

Answer: this is easy, i think

Explanation:

The volcano released a lot of sulfur dioxide and ash

According to question, "C" which is the volcano released a lot of sulfur dioxide and ash.

What is a volcano and what causes its eruption?

When magma builds up in the magma chamber, it forces its way up to the surface and erupts, often causing volcanic eruptions.

In the ocean, volcanoes erupt along cracks that are opened in the ocean floor by the spreading of two plates called a mid-ocean ridge.

Thus, the volcano released a lot of sulfur dioxide and ash is correct.

To learn more about volcanoes click here:

https://brainly.com/question/12945128

Which image shows karst topography?

Ocean with palm trees at the beach.

A sinkhole.

Overhead view of an oxbow and fields.

Answers

Answer:

B

Explanation:

a sinkhole is the answer. I got it on edge 2020

Answer:

its B

Explanation:

Which of these groups contains single-celled organisms?

A. birds
В. plants
C. fungus
D. animals

Answers

Answer:

C. Fungus

Explanation:

The fungi kingdom includes both single cell and multicellular organisms. Single cell organisms in the fungi kingdom include yeasts and chytrids, or fossilized fungi. Most organisms within the plant and animal kingdoms are multicellular. The Largest Single-Celled Organism.

hope i helped

Answer:

C. fungus

Explanation:

Birds and animals are in the same category, and both have multiple cells.

Plants also have multiply cells.

And fungus can be single celled or multiple cells.

Hope this helps ya!!

What was Edwin Hubble's contribution to the field of astronomy?

A. He discovered variable stars.

B. He proved that the heliocentric model accurately described our solar system.

C. He found a relationship between the period luminosity and distance of stars.

D. He proved that the universe is finite.

E. He discovered that the universe was expanding.

Answers

Answer:

B he proved that the heliocéntrico model accurately described tour solar system

Explanation:

des

i think

3. State and explain Chargaff's rules.

Answers

Answer: Chargaff's rules state that DNA from any species of any organism should have a 1:1 stoichiometric ratio (base pair rule) of pyrimidine and purine bases and, more specifically, that the amount of guanine should be equal to cytosine and the amount of adenine should be equal to thymine.

Explanation:

help meeeeeeeeeeeeeeeee plz

Answers

Answer:

It would most likely be within the mantle. the mantle is in the interior of the earth;. So inner core.

Explanation:

Please I beg someone to help me
Which most likely accounts for the increase in the number of male butterflies in the five years after the initial parasite problem?

Answers

Idk for sure but the first one seems like the best choice :)

how to write a narrative story?​

Answers

Answer:

Start with action or dialogue.

Ask a question or set of questions.

Describe the setting so readers can imagine it.

Give background information that will interest readers.

Introduce yourself to readers in a surprising way.

Explanation:

Answer:

Narrative essays/stories are often anecdotal, experiential, and personal—allowing students to express themselves in a creative and, quite often, moving ways. So, in short, it's an essay about your life with details, events, etc.

Explanation:

Narrative essays/stories should include:

an introduction, plot, characters, setting, climax, and conclusion.must have a purpose; think of this as the thesis of your story.the essay should be written from a clear point of view; essays to be written from the standpoint of the author (you); however, this is not the sole perspective to be considered. Creativity in narrative essays oftentimes manifests itself in the form of authorial perspective. narrative essays are effective when the language is carefully, particularly, and artfully chosen. Use specific language to evoke specific emotions and senses in the reader. the use of the first person pronoun ‘I’ is welcomed; though it is welcomed it is not necessary—nor should it be overused for lack of clearer diction.have a clear introduction that sets the tone for the remainder of the essay. Do not leave the reader guessing about the purpose of your narrative; you are in control of the essay, so guide it where you desire (just make sure your audience can follow your lead).

I hope that this helps you! I know it was a lot to read, but it's worth it. Have a nice night! :D

hello I need help!!! did I get this right ​

Answers

Answer:

Yeah it is correct.

Explanation:

Things enter the cell through the cell membrane through either active or passive transport which can tell you that the cell membrane controls what goes in and out.

I believe so. I looked it up and it matched everything I found

How does the waste of the pandemic relate to the biosphere?

Answers

Answer:

I think because there aren't a lot of people working, so there is no one to pick up after careless people.

That is the first thing that popped up in my head :\

:D

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