If bacteria transformed with a recombinant pUC18 plasmid produce white colonies when grown on plates containing ampicillin and Xgal, which of the following is least likely?

a. They produce β-lactamase that provides resistance to ampicillin.
b. They carry a vector that contains a DNA fragment inserted into the multiple cloning site.
c. They produce functional β-galactosidase that cleaves X-gal.
d. They carry a vector with a lacZ gene that has been disrupted and rendered nonfunctional.
e. They carry a vector with the bla gene used as the selectable marker.

Answers

Answer 1

Answer:

c. They produce functional β-galactosidase that cleaves X-gal.

Explanation:

β-galactosidase is an enzyme capable of catalyzing the enzymatic hydrolysis of β-galactosides into monosaccharides by hydrolyzing the β-glycosidic bond. On the other hand, X-gal is an analog of lactose composed of galactose, which is widely used in laboratories for the detection of β-galactosidase. In the presence of β-galactosidase, X-gal is hydrolyzed by cleaving its β-glycosidic bond, thereby confirming the presence of β-galactosidase.


Related Questions

3'T.A.C.C.A.T.A.T.T.G.A.G.5'

Answers

Answer:

a u g g u a u a a c u c

Explanation:

i think this is it, but there is no explanation of what to do

Our solar system is found near the inner edge of large Scutum-Centaurus Arm of the Milky Way. True or False?

Answers

Answer:

true

Explanation:

A/An ______________________________ is a specialized radiographic examination of the uterus and fallopian tubes.​ Question 94 options:

Answers

Answer:

Hysterosalpinography

Explanation:

It is Hysterosalpingography because it is an x-ray examination of uterus and fallopian tubes that uses a specific type of x-ray called fluoroscopy and a radiopaque material. It is use to examine women who find it difficult in getting pregnant to examine the shape of the uterus.

The linear strands of DNA in eukaryotes are efficiently packed within the nucleus of the cell. The packing of DNA strands is mediated by proteins called

Answers

Answer: Chromatin.

Hi! The answer you're looking for is chromatin.

Chromatin helps to organize DNA into the strands you're talking about, which are called nucleosomes.

Glad to help!

Identify each of the following characteristics or descriptions as Lycopodium sp. or Selaginella sp., using the following key. (Both may apply to some characteristics listed.)
L = Lycopodium S = Selaginella
They have rhizomes that branch horizontally, producing aerial stems and underground roots.
Their leaves have a ligule, or tongue, on the upper surface.
They are heterosporus
They are homosporus.
They have strobili.
They have simple, scalelike leaves on branching stems from which roots arise.
They are known as ground pine.
They are known as spike moss.
nionger dars for many things, Based on the name "scouring rush," what quality best

Answers

Answer:

They have rhizomes that branch horizontally, producing aerial stems and underground roots.  L

Their leaves have a ligule, or tongue, on the upper surface. S

They are heterosporus  S

They are homosporus L

They have strobili.  S L

They have simple, scalelike leaves on branching stems from which roots arise.  S

They are known as ground pine.  L

They are known as spike moss.  S

Explanation:

Lycopodium is known as as ground pine, and is a genus of clubmosses. They are homosporus, because they produce only one type of sporangia, while Selaginella are heterosporus, because they produce microspores and megaspores, and they are known as spike moss. Both of them, lycopodium and selaginella can have strobili.

Could an object, tool, or structure be made without the use of a natural resource? Explain your answer.

Answers

Bajamapa alsnbcian. Sin. A

After arousal by a sensory stimulus, consciousness can be maintained by positive feedback, because of activity in the

Answers

Answer:

This question lacks options, options are:

A) cerebral cortex.

B) basal nuclei.

C) sensory pathways.

D) motor pathways.

E) All of the answers are correct.

The correct answer is E.

Explanation:

The cerebral cortex processes and filters its information before passing the most relevant aspects to other regions of the brain. Some of these brain regions, in turn, send information back to the cortex. These loops, known as 'feedback systems', are considered essential for the functioning of cortical networks and their adaptation to new sensory information. Neural circuits must first assess the importance of incoming sensory information and then refine how it is processed in the future. Positive feedback, triggered with the purpose of amplifying the response to the initial stimulus, can be compared to a chain reaction or a vicious circle. Few are the functions regulated by this mechanism; rather it is triggered in pathological situations. It is the system by means of which the organism very rarely regulates any of the bodily functions under normal conditions, making the initial stimulus to be maintained and even increased. This type of mechanism is predominantly present in pathological situations: Its constitutive elements are: stimulus, receptor, afferent pathway, integrating center, efferent pathway, effector and response. The response does not have the ability to satisfy the initial stimulus.

The main evolutionary consequence of the process of 'plate tectonics' has been

Answers

The movement of plate tectonics produces new environments and gives organisms a new environment that they now need to adapt and evolve to fit into.

Where does cellular respiration get
the energy to make ATP? *
A. Chemical energy from sugars
B.Light energy from the Sun
C.Energy stored in the covalent bonds of
water molecules
D.Energy stored in the covalent bonds of
carbon dioxide molecules

Answers

Answer:

I believe A.

Explanation:

Cellular respiration gets energy to make ATP from the food you eat, so, A.

This category of plants does not have vascular tissue or seeds. Question 3 options: Angiosperms Gymnosperms Ferns Mosses

Answers

Answer:

Mosses

Explanation:

Mosses are the category of plants that does not have vascular tissue or seeds while angiosperm, gymnosperms, and ferns have vascular tissues and seeds.

Due to absence of vascular bundles or veins, the mosses also lack stems, true roots, and leaves. some of the examples of mosses are: liverworts, hornworts, and Ricceia natans etcetera.

Hence, the correct answer is "Mosses".

Answer:

Mosses

Explanation:

Mosses are the category of plants that do not have vascular tissue or seeds. Ferns do not have seeds, but do have vascular tissues. Angiosperms and gymnosperms have vascular tissue and seeds.

blood consists of which fluid medium
a.lymph
b.platelets
c.plasma
d.all of these

Answers

Answer:

C. Plasma

Explanation:

Blood consists of a fluid medium called Plasma.

Marking as brainliest

name another type of compound ( besides polysaccharides) that fits the definition of polymer

Answers

Answer:

A protein is also a polymer. The monomer in this case would be the subunits comprised of amino acids.

DNA. The subunits are nucleoTides.

Explanation:

Brainliest please

If you insert a gene from bacteria into a mouse genome, then which terms best explains what you just made?

Answers

Answer:

a transgenic animal

Explanation:

If the gene encoding one of the proteins that forms cohesin were mutated such that cohesin could not perform its normal function, what would be the consequence? Check all that apply.

Answers

Answer:

The following apply:

- Chromosomes would not be able to condense.

- Chromosomes would not be able to properly align at the metaphase plate.

- The separation of sister chromatids would not likely occur specifically during anaphase A.

- The nuclear envelope would not be able to reform.

The diagram shows a phase of mitosis. A phase of mitosis is shown. The chromosomes lined up at the center. Letter A represents long strings inside of the cell. Which does the letter A represent in the diagram?

Answers

I wish you added the diagram but I can tell that the long strings are the spindle fibres.

Answer:

the long string things are the fiber spindle thing

( sorry i'm not good at reading passages and citing from them... )

3. How would you describe the waxing versus the waning portion of the moon’s journey

Answers

Answer:

A lunar eclipse would occur when the Earth is between the sun and moon. ... 3How would you describe the waxing versus the waning portion of the moon's journey? The waxing is when the moon is “growing”, while the waning is when the moon is “shrinking”.

How well do you think the exercised muscles would work after 30 minutes of rest instead of 30 seconds of rest between trials?

Answers

Answer:

We at Bright Side invite you to take a look at what you can expect from your body when you finally pull yourself together and start training. Illustrated by Ekaterina Gapanovich for Bright Side.

Explanation:

Possibility of causing unnecessary stress and strain on your muscles, especially your heart.

What if I didn't warm-up exercises before doing any physical activities?To help prevent injury, include static stretches in your cool-down routine. Static stretching as part of a maintenance stretching program can also help lower your risk of injury.Possibility of causing unnecessary stress and strain on your muscles, especially your heart. Inability to prepare the pathways between your nerves and muscles for a good workout. Inability to increase blood flow into muscle groups, which is critical for delivering oxygen and nutrients.Stretching is relaxing because it activates the parasympathetic nervous system and increases blood flow to the muscles.

To learn more about : Static stretching

Ref : https://brainly.com/question/1297773

#SPJ2

Question 8 of 10
What three things most directly affect carrying capacity?
A. Wood, electricity, and water
B. Money, medicine, and food
C. Water, money, and technology
D. Food, space, and water
SUBMIT

Answers

Answer:

food space and water

Explanation:

Which structure in plants produces a substance that protects the extending roots from developing friction?

a.root cap
b. stele
c.stem
d.epidermis

Answers

Answer:

The correct answer is - option a. root cap.

Explanation:

Roots of the plants are developed from the structure called radical in seed during germination of seed.The tip of the root is tender due to the present of the apical meristem that is the precursor of the new cells of the root and protected by the root cap and penetrate in to the soil with the help of mucigel. Mucigel is the which protects the extending roots from developing friction.

Thus, the correct answer is : option a. root cap.

Answer:

The answer is ROOT CAP

Explanation:

Got it right on my test.

What is the maximum magnification of most classroom compound light microscopes? 500x 1,000x 2,500x 5,000x

Answers

Answer:

2,500

Explanation:

Answer:

the answer is 2,500x

Which properties of systems does a robot have? Which does it not have?

Answers

Answer:

Robot has physical structure, movable and brain.

Robot has no feelings and no ability of reproduction.

Explanation:

A robot have the following properties of systems such as they have a physical structure which helps them to move from place to place. They also have a sensor system which detect any change in the environment, a power supply which helps them for doing different activities and a computer brain which control all the activities. A robot does not have any feelings such as pain, happiness etc because it is a machine and inability to reproduce like humans.

Soil moisture that plants are capable of accessing and using is called Group of answer choices capillary water.

Answers

Answer:

Explanation:hv

in the fruit fly, the long wing allele is dominant over the short wing allele. What are the parental genotypes for a cross that yields 75% long winged offspring and 25% short winged offspring.

Answers

According to the scenario, the parental genotypes for a cross that yields 75% long-winged offspring and 25% short-winged offspring are Ll and Ll.

What are Genotypes?

Genotypes may be defined as the ultimate combination of alleles of those genes which are significantly taken for specific studies.

According to the context of this question, in the fruit fly, the long-wing allele is dominant over the short-wing allele. This means that capital L is dominant over small l. So, the genotypes LL and Ll govern the dominant trait, i.e. Longwing.

The Punnett square for the given cross is as follows:

Therefore, the parental genotypes for a cross that yields 75% long-winged offspring and 25% short-winged offspring are Ll and Ll.

To learn more about Genotypes, refer to the link:

https://brainly.com/question/1626661

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why does mosquito net easily form lather with hard water ​

Answers

Answer:

Hard water is defined as the type of water that does not readily give a good lather with soap.

Mosquito net do not form easily lather with hard water because hard water is made up of magnesium and calcium ions which precipitate the soap and unable to form lather.

Which statement best describes geothermal energy? Geothermal energy can be used to fuel vehicles. Geothermal energy increases the risk of a nuclear accident. Geothermal energy is the most abundant nonrenewable resource. Geothermal energy can be used as a source of electricity.

Answers

Answer:

Geothermal energy

Explanation:

can be used as a source of electricity. hope this helps you :)

Answer:

D

Explanation:

Is lead renewable or not?

Answers

Answer:

it is not renewable.........

Answer:

Lead is not a renewable resource. It is a metal that forms naturally in the Earth, but it takes a very long time for metals to form.

b) How will you describe any three (3) major components of the environment to a named
class puyil?​

Answers

Answer:

Hydrosphere, atmosphere and biosphere are the three major components of the environment.

Explanation:

Hydrosphere, atmosphere and biosphere are the three major components of the environment. hydrosphere refers to water bodies such as ocean, sea, ponds and lakes etc that is present in our environment. atmosphere refers to the gaseous layer which is present above the earth surface. in this layer oxygen, nitrogen and carbondioxide etc are present. biosphere refers to all living organisms such as human, animals, plants and microbes etc which are present on earth surface..

Using specific examples, discuss the roles of negative and positive feedback processes that maintain homeostasis.

Answers

Answer:

Homeostasis typically involves negative feedback loops that counteract changes of various properties from their target values, known as set points. In contrast to negative feedback loops, positive feedback loops amplify their initiating stimuli, in other words, they move the system away from its starting state.

Bats have oversized ears, which help the bad you sound waves to detect the motion of their prey which example of the characteristics of life is most similar

Answers

This question is incomplete because there are no options; here is the complete question:

Bats have oversized ears, which help the bad you sound waves to detect the motion of their prey which example of the characteristics of life is most similar

A) A yeast cell splits itself in half to create a new organism.

B) A rat hides in its hole when it sees an approaching predator.

C) A fungal cell releases carbon dioxide back to the atmosphere.

D) A human child grows taller every year.

The answer to this question is B) A rat hides in its hole when it sees an approaching predator.

Explanation:

One of the characteristics of living organisms is the ability to adapt or respond to the environment. This feature of life is the one shown by bats in this case because the oversized ears are a result of evolution and adaptation that help bats to have more chances of survival. Similarly, rats hiding to avoid predators is a result of adaptation that helps rats survive by avoiding predators. Thus, rats and bat show the same characteristic of life.

A researcher discovers that polar bears have very good insulation and very think hair (similar to pandas). However, they do not show the rest of the panda behaviors. For example, they have high daily energy expenditure and are very active. Why are the panda and polar bear so different

Answers

Answer:

Panda and polar bear have some similar characteristics but they also have differences in their behaviors such as polar bears have high daily energy expenditure and are very active while pandas are not.

The panda and polar bear so different because of the geographical difference where they live.

Polar bears are marine mammals and live in north pole perform swimming in north oceans and also move on ice flows, where they catch seals for survival, it makes them active and energetic.

While Pandas are found in China bamboo forests and are herbivores. They eat leaves from bamboo trees, for which they do not require to be more active and get their food easily. So, Pandas are not active as polar bears.

As the climate of North pole and China is cold, both polar bear and panda have very good insulation

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