Insulin is a hormone produced by some pancreatic cells. Scientists have isolated the DNA sequence that codes for human insulin production Which of the following best predicts the effect of inserting this gene into the DNA of a bacterial cell?
А) The recombinant bacterium will produce human insulin using its own transcription and translation machinery.
B) The recombinant bacterium will not transcribe the human insulin gene because this gene is not normally found in the bacterial genome.
C) The recombinant bacterium will transcribe the gene but will be unable to translate the mRNA.
D) The recombinant bacterium will die because it has been exposed to foreign DNA.

Answers

Answer 1

Answer:

А) The recombinant bacterium will produce human insulin using its own transcription and translation machinery.

Explanation:

Recombinant DNA technologies refer to molecular biology techniques used to create chimeric DNA sequences by using genetic recombination techniques. The resultant recombinant DNA can further be used for the production of desired gene products (proteins) both in vitro and in living organisms. DNA recombinant techniques involve the insertion of desired DNA regions into an appropriate vector such as, for example, in a plasmid that is amplified in bacteria. For this purpose, the desired DNA fragment is inserted into a plasmid vector by digesting both the target DNA and plasmid insertion sites with an appropriate restriction enzyme. Subsequently, a DNA ligase is used to join both DNA fragments from their ends. Finally, the transformed bacterial (host) cells are selected to express the desired inserted gene.


Related Questions

Which statement describes the relationship between the deer and plants on the food web? (3 points) Tree is consumed by insect, which is consumed by the frog and lizard. The frog and lizard is consumed by the snake and the snake is consumed by the hawk. The mayfly is consumed by the fish and the fish is consumed by the bear. The plants are consumed by the deer and bear. The deer is consumed by the wolf. Group of answer choices Heterotrophs supply food for the autotrophs. Autotrophs and heterotrophs share the same food source. Autotrophs supply food for the heterotrophs. Autotrophs and heterotrophs compete for the same food source.

Answers

Answer is B - Autotrophs supply food for the heterotrophs.

A flower would be a type of autotroph and the deer would be a type of heterotroph. It would only make sense if it was B.

Also I took this test already on Canvas, and got it correct.

A basic principle for scientific research is that the experiment must be able to be replicated. What happens when an experiment is replicated?

a) A scientific publisher accepts the experimental results for printing.
b) No one in the scientific community agrees with the methods or results.
c) Other scientists perform the same experiment and get comparable results.
d) The scientific researcher performs the experiment many times and gets the same results.​

Answers

Answer:

i think it might be C sorry if i'm wrong if i'm right can you give me brainliest i need it

Explanation:

Answer:

Other scientists perform the same experiment and get comparable results.

Explanation:

Help pls !!

How do increases in the carrying capacity of Earth for people affect the carrying capacity of Earth for other species?

1. It increases for all species .

2. It decreases for all species .

3. It increases for most species

4. It decreases for most species .

Answers

Answer:

It decreases capacity for other species

Explanation:

As humans clear and develop more and more land, the animals that live in the cleared land lose their homes, so they have nowhere to live. This decreases the animals carrying capacity while increasing humans carrying capacity.

The knob like structures present on the hyphae are referred as (1)

a) Spores

b) Sporangium

c) Fronds

d) Sori​

Answers

[tex]\huge\star{\underline{\mathtt{\red{A}\pink{N}\green{S}\blue{W}\purple{E}\orange{R}}}}\star[/tex]

[b] The sporangia are knob-like structures.

They are present at the top of the thread-like structures called hyphae.

They are dispersive, i.e. they are scattered by rain, wind or insects.

I need help with the answer

Answers

Answer:

Carbon Dioxide

Explanation:

The light-independent reactions use the ATP and NADPH from the light-dependent reactions to reduce carbon dioxide and convert the energy to the chemical bond energy in carbohydrates such as glucose.

In which phase of cellar respiration is glucose a substrate?

Answers

Answer:

glycolysis

Explanation:

Answer:

answer of this question is glycolysis

:-))

Decision as to the cause or nature of a disease given by the M.D.
_____________.
it gives no choices but this is medical terminology
please help

Answers

I think it’s etiology...

Indicate whether each statement is true or false. 1. Rod cells are depolarized in the dark. (Click to select) 2. Glutamate binds to receptors on bipolar cells and causes them to depolarize. (Click to select) 3. Light and dark adaptation are accomplished by changes in the amount of available iodopsin. (Click to select) 4. When light reactions occur, retinal is completely released from opsin. (Click to select)

Answers

Answer:

The correct answer is - 1. true, 2. true, 3. false, and 4. true.

Explanation:

1. Rod cells are the photoreceptors present in the retina that are hyperpolarized not depolarized in dark due to sodium ions efflux.

2. Depolarisation of the bipolar cell of the retina by glutamate release by rods and cones.

3. The adaption according to the light is known as a light adaptation which takes place due to a large part of photochemical in both rods and cones which is reduced to retinal and opsins.

4. when the light reaction takes place retinal converted to opsin

Rod cells are photo-receptors that present in the retina. They hyper-polarized  in the dark due to sodium ions efflux. Statement 1, 2, and 4 are correct while statement 3 is incorrect

 

1) Rod cells are photo-receptors that present in the retina. They hyper-polarized  in the dark due to sodium ions efflux.

2) Depolarization of the bipolar cell of the retina is caused by glutamate released from rod and cone cells.

3) The changes according to the light is known as a light and dark adaptation which is accomplished by the change in oidopsin.

4) In the light reaction takes place retinal converted to opsin,

Therefore, Statement 1, 2, and 4 are correct while statement 3 is incorrect.

To know more about Rod cells,

https://brainly.com/question/15826713

I need help it’s hard help please

Answers

A it True it’s not really hard
the answer is true!

cellular respiration takes place in all organisms

1. Which membrane-bound organelle contains the genetic material of the
cell (such as DNA)?*
O Cytoplasm
Nucleus
Mitochondria
Endoplasmic Reticulum (ER)

Answers

The Nucleus!





...... I need brainliest

Answer:

Okay h

Explanation:

Which type of pollution is caused by the use of excess nutrients and pesticides?

Water

Soil

Air

Crop

Answers

Answer:

Water

Explanation:

This is called Eutrophication. This happens when extra nutrients seep into water causing algae and other organisms to grow.

Brainliest?

in monohybrid cross between a pure breed plant that produces blue flowers (BB)and a pure breed plant that produces white flowers.The f1 generation produces only blue flowers. By means of a labelled cross diagram,state the type of flower you will expect if the f1 generation is self pollinated​

Answers

Answer: If the F1 generation is self-pollinated, 75% of the flowers will be blue, while 25% will be white.

Explanation:

Monohybrid cross between blue and white: BB x bbThe cross produces 100% Bb --> blue is dominant, so all the flowers will be blue. This is the F1 generation.If F1 is self-pollinated, it will be Bb x Bb.The cross will produce 25% BB, 50% Bb, and 25% bb.Since blue is dominant, 75% of the flowers will be blue, while 25% will be white.

Pictures:

The first picture is the BB x bb cross.The second picture is the Bb x Bb cross.I don't know why the pictures are appearing sideways. If you click the rotate button at the top right of the pictures, you can turn them.

After this, rRNA creates bonds between _____________ to make __________

Answers

Answer:

After this, rRNA creates bonds between amino acids to make proteins.

Some important points to know:

rRNA (Ribosomal RNA) is used in the synthesis of proteins.

Amino acids are the building blocks of proteins which means that proteins are made up of amino acids.

When amino acids are joined together, they form proteins.

The bond between two or more amino acids when bonded is called "peptide bond".

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Hope this helped!

~AH1807

Answer:

rRNA  bonds with mRNA which creates peptide  bonds between amino acids creating PROTEINS

Explanation:

• Describe the distribution of electrons around the nucleus of an atom

Answers

Answer:

Viewed simply, electrons are arranged in shells around an atom's nucleus. Electrons closest to the nucleus will have the lowest energy. Electrons further away from the nucleus will have higher energy. ... In a more realistic model, electrons move in atomic orbitals, or subshells.

Explanation:

One nucleotide in a DNA strand is replaced with another, but the change results in
the same amino acid to be placed next in the protein. What is this type of mutation?

nonsense
point
silent
frameshift

Answers

Answer:

The type of mutation that occurs when one nucleotide in the DNA strand is replaced by another, but the change results in  the same amino acid to be placed next in the protein is a silent mutation.

Explanation:

Silent mutations are molecular mutations where, despite a change in one of the DNA nucleotides, this change has no impact on the amino acid sequence that a synthesized protein will have.

An example of a silent mutation is the change in a DNA sequence TTC where a thymine replaces the cytosine, obtaining the TTT sequence:

                  Normal     With mutation

DNA              TTC            TTT

mRNA           AAG           AAA

Amino Acid   Lys             Lys

There was a nucleotide change in the DNA, but the encoded amino acid was the same, which is a silent mutation that does not affect the amino acid sequence of a protein, its structure or function.

A student is investigating how substrate concentration affects the rate of anenzyme-catalyzed reaction. Which ​threevariables​ should be kept constant in the setup of thestudent’s investigation? Select all three variablesA. temperature of the reactionB. amount of enzymeC. amount of substrateD. amount of productE. pH of the reaction

Answers

Answer:

A. temperature of the reaction

B. amount of enzyme

E. pH of the reaction

Explanation:

Temperature should be kept constant because enzymes operate at different activity rates depending on the temperature. The same goes for the pH as well with different enzymes having different optimal pH levels.

The amount of enzyme should be kept constant as well because different concentrations of enzymes can determine how fast the reaction will occur.

Essentially anything that could affect enzyme activity apart from substrate concentration should be kept constant.

What happens in cytokinesis?

Answers

Answer:

Cytokinesis is the physical process of cell division, which divides the cytoplasm of a parental cell into two daughter cells. ... The contractile ring shrinks at the equator of the cell, pinching the plasma membrane inward, and forming what is called a cleavage furrow.

Explanation:

hehe plss give me a heart

Answer:

The cell splits into two.

Explanation:

Cytokinesis is the process of cell division which divides the cytoplasm of the parent cell into two daughters.

The cell splits into two.

Which of the following is part of the structure of a fruit? glume monocot mesocarp cotyledon

Answers

The answer is mesocarp


In fleshy fruits, the pericarp is typically made up of three distinct layers: the epicarp (also known as exocarp), which is the outermost layer; the mesocarp, which is the middle layer; and the endocarp, which is the inner layer surrounding the ovary or the seeds.

Answer:

Mesocarp

Explanation:

Explain what causes chromosomal disorders.

Answers

Answer:

Explanation:

Some chromosomal conditions are caused via way of means of modifications within side the range of chromosomes. These modifications aren't inherited, however arise as random occasions for the duration of the formation of reproductive cells (eggs and sperm). An blunders in cell department known as nondisjunction effects in reproductive cells with an extraordinary range of chromosomes.

yw :)

Isostasy leads to isostatic adjustment if mass is redistributed. Erosion redistributes rock from the mountain to the sediment in the
basin. Which statement does NOT reflect what occurs when erosion happens?
-)))
A)
Mass increases at the crust.
B)
Less mass on the mountain causes uplift.
0
The crust beneath the mountain thins and rises.
D)
The mass of the crust decreases and the mass of the mantle increases.

Answers

Answer: It's A.

Explanation: I am doing UsatestPrep, Now I got this answer wrong, but afterwards it told me the right answer is! "Mass increases at the crust" I got the answer wrong originally because I chose B but it's actually A.

Answer:

Its A because Mass increases at the crust. . Mass is actually decreasing due to erosion of the crust, isostatic adjustment will thus, cause the mantle mass to increase over time.

Explanation:

3. Which of the following is a chemical property
(10 Points)
melting point
Ostates of matter
density
flammability
4. Substances formed as a result of a chemical reaction are called *

Answers

Answer: The answer is flammability

Explanation:

because it is a chemical thing happening

Electron microscopes can reveal details

Answers

Answer:

Answer is on the pic

Explanation:

I hope it's helpful!

Which two processes are responsible for the formation of fog?

Answers

Answer:

Explanation:

evaporation

18) What is the difference between eumelanin and pheomelanin?

Answers

Answer:

Eumelanin is black or brown pigment and pheomelanin is red or yellow pigment

7. How do the data support your claim above?
Explain your statement above. Be sure to refer to specific pieces of data from your experiment that
support your argument. HELP

Answers

Well what’s the data

Why is it important for the cells produced in meiosis to be haploid?

Answers

Answer:

The overall process of meiosis produces four daughter cells from one single parent cell. Each daughter cell is haploid, because it has half the number of chromosomes as the original parent cell. ... Unlike in mitosis, the daughter cells produced during meiosis are genetically diverse.

Answer:

A.) Because bacteria only need half the DNA to survive and they are the only ones that do meiosis.

Explanation:

4. Take a beaker (jar or jug) with some water and put some drops of red or blue
ink in it. Take a tender twig of any flowery plant with leaves and flowers, preferably
white flowers, and put it in the beaker for 6 to 8 hours. What do you observe? For
further examination cut across its stem and look for the presence of colored water.
Explain your observations.​

Answers

Answer:

Blue Ink patches are observed on the leaves of the plant. Also, on cutting across the stem of the plant, the red color of the solution is found along the length of the stem.

The above observation shows that the stem of the plant conducts water through its length to the leaves of the plant.

Explanation:

The leaves of plant obtain water and minerals from the soil through the roots of the plant. The water is conducted by the stem of the plant from the roots to the leaves of the plant where they are needed for photosynthesis as well as for cooling the plant. The xylem tissue in plants is responsible for conducting water to the leaves of the plant from the roots of the plant through the stem.

The conduction of water from the roots to the leaves of the plant is maintained by capillary action as well as by transpiration pull. Transpiration pull refers to the pull of water by the transpiration action taking place in the leaves of the pant, whereby water evaporates from the leaves of the plant through the opening in the leaf of the plant.

When the blue ink is added to the water, the colored water is conducted through the stem of the plant to the leaves. On getting to the leaves, the water molecules evaporate leaving behind the colored patches fond on the leaves. When the stem of the plant is cut, the blue color is seen along its length showing that the colored water was conducted through it.

PLS HELP how does gene knockout work?

Answers

Gene knockout is a method involves creating a DNA construct containing the desired mutation. For knockout purposes, this typically involves a drug resistance marker in place of the desired knockout gene. ... This method then relies on the cell's own repair mechanisms to recombine the DNA construct into the existing DNA.

How does DNA determine an organism’s traits

Answers

Answer:

Traits are determined by genes. A gene is the basic physical and functional unit of heredity. Genes are made up of DNA. Some genes act as instructions to make molecules called proteins.

Use the context of the urinary system to explain the term osmoregulation

Answers

Answer:Kidneys regulate the osmotic pressure of a mammal's blood through extensive filtration and purification, in a process known as osmoregulation. Kidneys filter the blood; urine is the filtrate that eliminates waste from the body via the ureter into the bladder.

Explanation:

Kidneys regulate the osmotic pressure of a mammal's blood through extensive filtration and purification, in a process known as osmoregulation. Kidneys filter the blood; urine is the filtrate that eliminates waste from the body via the ureter into the bladder.

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