Often in laboratory experiments you will want to amplify regions of the genome from several different preparations of gDNA. To do this, a mastermix will be created with all the common reagents to ensure easier pipetting, proper mixing, and controlling for variations in pipetting. Create a mastermix for running 8 identical reactions, including one negative control, each with different gDNA templates (7 total gDNA templates).

Answers

Answer 1

Answer:

Mastermix in PCR helps in providing faster and easier setup with pipetting, proper mixing, reduction in contamination and controlling fir variation in pipeting or tube to tube variability. Contents of PCR master mix for the desired reaction are as follows:

dNTPsPrimersthermostable DNA polymerasecofactor (MgCl2)Template DNA (for 7 samples)PCR-grade water

The only difference in negative control for the PCR master mix is template or the different primer rest the components are the same in the master mix.


Related Questions

Please help!!!!!!!!!!!!!
Some organisms, such as bacteria, reproduce asexually; whereas, other organisms reproduce
sexually. Which statement correctly states why one type of reproduction is beneficial over the
other?
A. Asexually reproduced offspring are more genetically diverse.
B. Asexual reproduction produces offspring genetically different from parents.
C. Sexual reproduction can produce a large number of offspring very quickly.
D. Sexual reproduction produces organisms that are genetically diverse.

Answers

Answer:D

Explanation: A isn't right because during asexual reproduction there is no exchange of genetic characteristics thus the offsprings retain the genetic characteristics of their parents and there is no diversity.

Sexual reproduction on the other hand involves the fusion of gametes and the exchange of genetic characteristics thus the offsprings are diverse and are not identical to their parents as is the contrary in asexual reproduction.

Sexual reproduction produces organisms that are genetically diverse.

Sexual reproduction involves the combination of chromosomes from both parents. This combination of chromosomes introduces variation into the population. This combination does not occur in asexual reproduction.

Hence, the statement that correctly states why one type of reproduction is beneficial over the  other is that sexual reproduction produces organisms that are genetically diverse.

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1. A solution has 65% water. A Cell has 65% solute. Which way does the water
move: into or out of the cell, or no net movement?
2. Is the solution from question 1 hyper- hypo- or isotonic compared to the cell?
3. Will the cell shrink, swell, or stay the same?

Answers

Answer:

the water will enter into the cellthe solution is isotonic
i think the answers is

a ) the water will move into the cell

b ) the solution is more hypotonic rather than the cell . thus the water will enter the cell.

c ) the cell will swell because water enters the cell.

i am sorry if my answers were wrong .

The mass of a proton?

Answers

☁️My Answer☁️:

The mass of the proton is the sum of the mass of current quarks and the binding gluons. The mass of the proton is-

⬇️

Proton mass mp = 1.672621898(21)×10−27 Kg

The Mass of proton is higher than the mass of electron.

☼Mass Of Proton In Other Units☼:

The mass of the proton can be expressed in alternative units like – proton mass AMU, the mass of the proton in kg, proton mass MeV. The table given below comprises the value of proton mass and corresponding units.

✯Proton mass✯:                             ∞Value:

Mass of the proton in kg                  1.672621898(21)×10−27 kg

Mass of the proton in grams            1.672621898(21)×10−24 g      

Mass of proton in AMU                    1.007276466879(91) u

                                               

Proton mass MeV                           938.2720813(58) MeV/c2

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I hope this helped you,

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what is meant by the term VO2 max

Answers

Answer:

VO2 max is the maximum volume of oxygen you can consume per minute per kilogram of body weight at your maximum performance. VO2 max is an indication of aerobic fitness and should increase as your level of fitness improves.

Answer:

VO2 is the maximum oxygen uptake.

Explanation:

VO2 is the maximum oxygen consumption during incremental exercise.

what must happen before miosis can begin?

Answers

Answer:

Before meiosis actually begins, the DNA that is packaged into chromosomes must be fully copied. ... DNA replication occurs in the same fashion as it does during mitosis. After replication, the homologues are doubled, and each chromosome now has a homologous pair

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How is an occluded front different from a cold front?
a) Warm air is pushed up during an occluded front, while cold air moves quickly during a cold front.
b) Occluded fronts cause warm air to feel dry, while cold fronts cause ground temperatures to feel cooler.
c) Warm air moves slowly during an occluded front, while cold air is cut off from the ground in a cold front.
d) Occluded fronts cause rapid changes in weather conditions, while cold fronts cause widespread cloud formation.

Answers

Answer:

A cold front forms at the leading edge of the colder air mass the cold front can bring thunderstorm in the region whereas the occluded front can bring strong winds and precipitation. so its probably d

Occluded fronts cause rapid changes in weather conditions, while cold fronts cause widespread cloud formation. Therefore, option (D) is correct.

What are occluded fronts?

Because of their different densities, large masses of warm air and cold air do not mix when they come into contact with one another. They instead organize themselves into a front that is typically hundreds of miles long. When a warm air mass is sandwiched in between two cold air masses, the result is a phenomenon known as an occluded front. The mass of warm air rises as the masses of cool air push against one another and meet in the middle. The warm air mass is occluded, which literally means "cut off," from the ground, and pushed upward, which causes the temperature to drop. These types of fronts are known to bring blustery winds and significant precipitation.

Occluded fronts typically develop near low pressure areas that have reached their mature state.

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According to scientists, all of the bodies in the universe came from a rotating cloud of gases and dust about
billion years ago.

O 3.5
O 2.5
O 4.5
O 1.2

Answers

It's either 3.5 or 1.2

There would be more evidence spread out through space to be noticed by scientists and it seems like a stretch to go as far back as 4 billion years ago.

(I mean really? That much before the DINOSOURS died? I don't think so)

◕‿◕

Hope this helps

Miri

would an electric eel be able to shock predators if its cells run low on ATP?

Answers

Answer: no.

Explanation:

No because it would need to run energy to be able to shock its predators

what do you think will happen if u certain part of the cell fails to function​

Answers

Answer:

If that happens, you body will die soon

Explanation:

explain how precipitation affects the phosphorus cycle

Answers

Answer:

Disrupts the nitrogen cycle by contaminating the groundwater and making it more difficult for the plants to absorb the nitrogen and causes the phosphorus cycle to accelerate, resulting in an excess of phosphorus in water and soil.

Explanation:

The precipitation affects the phosphorus cycle in many ways where the soil erosion will take place if the precipitation happens then the weathering of rocks along with the soil getting off will make the nutrients loss in the soil.

What is the cycle of rain called as ?

The cycle of the rain is  called as water cycle where the evaporation along with the condensation takes place.

If the precipitation level in the atmosphere is increased then in this case it will increase the transportation of phosphorus along with the other nutrients into soil along with the water sources and the coastal areas as well.

The main reasons of such scenario is because of the deforestation, loss of habitat by the continued invasion of human activities in the natural resources. The reduction of nutrients loss in  soil makes an huge impact over the  phosphorus cycle.

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If a species has a 2n number of 18, how many autosomes would their SOMATIC cells have?

Answers

Answer:

If a species has a number of chromosomes 2n = 18, then its somatic cells have 16 autosomes.

Explanation:

Somatic cells are diploid, that is, they have the complete chromosomal charge, and it is represented as 2n. In organisms with sexual reproduction the chromosomes are divided into autosomes and sex chromosomes, which are 2.

If a species has a number 2n = 18, it means that its somatic cells have 18 chromosomes, of which 16 are autosomes and 2 are sex chromosomes. Autosomes contain the structural and functional characteristics of an individual, while the sex chromosomes determine sex.

In Labrador retrievers, black coat color is dominant to brown. Suppose that a black Lab is mated with a brown one and the offspring are eight black puppies. What is the most likely genotype of the black parent

Answers

Dominant because dominant genotypes overcome recessive genotypes

The genotype of the parents of black puppies are dominant. The two parents can be homozygous (BB) or heterozygous (Bb) dominant.

What is Genotype?

The genotype of an organism refers to the complete set of genetic material in an organism. Genotype can also be used to refer to all the alleles or variants an individual carries in a particular gene or genetic location in the DNA.

In labrador dogs, black coat color is dominant over brown coat color. If black labrador is mated with brown labrador and the offspring produced all eight are black colored that is dominant. Then, in this case the genotype of the parents will be:

If all the offspring are black colored, this means that each puppy has at least one dominant allele 'B' in their genotype. So, it can be predicted that both the parents should be at least heterozygous for this character. If both the parents are heterozygous then, some of the offspring can have brown color coat as well. So, the parents should both be homozygous or one homozygous and one heterozygous for this trait.

This can be shown through punnett square.

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Which type of speciation is based on a small number of individual being
introduced into a new environment?

Answers

Answer:

peripatric speciation

Explanation:

When small groups of individuals break off from the larger group and form a new species, this is called peripatric speciation

HELP!!!! Two characteristics of most plants are a root system and a shoot system. Most of the structures in these systems
are common to all plants; however, there are some variations. Which structure can sometimes be found as part of
the shoot system?
stems
leaves
roots
flowers

Answers

Answer:

Leaves, and flowers

Explanation:

The shoot system is above ground and includes the organs such as leaves, buds, stems, flowers (if the plant has any), and fruits (if the plant has any).

Answer:

ROOTS

Explanation:

ROOT ROOT ROOT ROOT ROOT ROOT because it root

Energy is defined as the ability to
A. reproduce.
B. grow.
C. do work.

Answers

Answer:

its c

Explanation:

Answer:

C. to do work

Explanation:

A scientist is developing a potential treatment for cancer. She grows cells in hundreds of petri dishes and exposes the dishes to environments that are known to cause cancer. After exposure, the scientist determines that some of the petri dishes have cancer cells.What factor do all the petri dishes that contain cancer cells have in common?

Answers

Answer:

they are normal mutations you can never have a cure to cancer you would have to throw a lot of your DNA and genes away to have a less chance of dying but we could slow or kill cancer but no way to prevent it from happning as it happens everyday to you but those cells have a higher chance to become cancer becuase of those thing that help cells to become cancer.

Explanation:

what is the value of 6a + 3

Answers

Answer:

3 ( 2 + 1 )

Explanation:

A man is at high risk for having a heart attack, the offspring will face risks as well.this is an example of.

Answers

Explanation:

this is hereditary. the male is carrying bad genes and if they are dominant then the offspring will most likely get it too. I hope this helps!!!

Please help I’m begging you please

Answers

Answer:

g

Explanation:

Answer:

A.

Explanation:

Because when the wolf numbers are low the deer numbers are high which means that the wolves have not been eating the deer, possibly because of a lack of wolves.

WILL MARK BRAINLIEST! :)
Diversity occurs in many places in meiosis. In what process is the first place where the pieces of DNA switch positions with the homologous sister chromatids?

A. Interphase

B.Independent Assortment

C. Crossing Over

D.Anaphase

E. Randomness of egg and sperm meeting

Answers

Answer:

crossing over

Explanation:

I had it on a bio exam. i got it right


Horses with genotype (BB) have a brown coat, horses with genotype (bb) have a white coat,
and horses with genotype (Bb) have a tan colat. Based on the information provided, the
alleles controlling coat color in horses show:

Answers

Answer: Incomplete dominance

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