Predict the most likely outcome of culturing transformed E. coli in the presence of tetracycline.Justify your prediction.
Answers
Answer:
E.coli is a bacterium which is preffered for transformation due to its rapid growth and high efficiency of introduction of DNA molecules into cells.
Tetracycline is an antibiotic used to treat several infections such as cholera, plague, brucellosis, and malaria etcetra.
Plasmid is a small, extrachromosomal DNA molecule present in cell which is seprated from chromosomal DNA and have ability to replicate independently.
When a plasmid resistant to tetracycline and kanamycine is inserted into E.coli, the process is called transformation. E.coli might not be able to survive due to absence of plasmid but the transformed strains having antibiotic tetracycline and kanamycine will probably be able to survive it.
Hence, the culture of bacteria carrying plasmid will be able to survive only.
Maximum transformation to tetracycline achieved when cells were harvested from L broth at 5.0-6.0 cfu/ml , followed by washing twice in cold.
The susceptibility of E.coli strain to culturing transformation by plasmid DNA was examined and optimized.
TransformationCells grown within the presence of D - cycloserine ( Cyc ) rather than Neg Gram (N x) transformed better. The presence of 5 mm Mg2+ ions in washing and CaCl2 solutions stimulated transformation about 2-fold.
Optimal conditions for transformation included a pH range of seven. 25-7.75 and a cell-to-DNA ratio of about 1.6 . 10(8) cfu/ng plasmid DNA.
The frequency of transformation was highest when cells were exposed to 100 mM CaCl2 in 250 mm.
Composition[tex]\rm KCl + 5 mM \;MgCl2 + 5 mM \;Tris[/tex]
pH 7.6, before mixing with DNA
Therefore, the results of culturing transformed E.coli within the presence of tetracycline is justified.
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Related Questions
What is a “shared derived character”?
Answers
Answer:
A shared character is one that two lineages have in common, and a derived character is one that evolved in the lineage leading up to a clade and that sets members of that clade apart from other individuals.
Answer:
A shared character is one that two lineages have in common, and a derived character is one that evolved in the lineage leading up to a clade and that sets members of that clade apart from other individuals. Shared derived characters can be used to group organisms into clades. For example, amphibians, turtles, lizards, snakes, crocodiles, birds and mammals all have, or historically had, four limbs. If you look at a modern snake you might not see obvious limbs, but fossils show that ancient snakes did have limbs, and some modern snakes actually do retain rudimentary limbs. Four limbs is a shared derived character inherited from a common ancestor that helps set apart this particular clade of vertebrates.
Which statements about mutations are true? Check all that apply.
A. Mutations cannot be passed from generation to generation.
B. Mutations are rare.
C. Mutations are always harmful.
D. Germline mutations occur in body cells.
E. Most forms of cancer are caused by somatic mutations.
Answers
Answer:
most forms of cancer are caused by somatic mutations
Explanation:
Answer:
B & E
Explanation:
Just did it and got it right
A gene mutation is when chemical changes occur in DNA of individual cells. True or False
Answers
Answer:True
Explanation:
Answer:
true
Explanation:
Identify the type of energy described in each sentence.
gravitational energy
mechanical energy
chemical energy
electrical energy
thermal energy
nuclear energy
radiant energy
The body stores lipids ingested as fat and uses this energy when needed.
A person’s hat falls off and lands on the ground.
A wave of water pushes a surfer to shore.
A burner on a stove heats up a tea kettle.
A power plant splits atoms to generate power for a city.
A nerve sends an impulse to another nerve.
A plant absorbs the Sun’s rays to start the process of photosynthesis.
Answers
Answer:
chemical energy: The body stores lipids ingested as fat and uses this energy when needed.
gravitational energy: A person’s hat falls off and lands on the ground.
mechanical energy: A wave of water pushes a surfer to shore.
thermal energy: A burner on a stove heats up a tea kettle.
nuclear energy: A power plant splits atoms to generate power for a city.
electrical energy: A nerve sends an impulse to another nerve.
radiant energy: A plant absorbs the Sun’s rays to start the process of photosynthesis.
Explanation:
Which organisms would have the most similar traits?
Answers
Answer:
Organisms in the same family will have the most similar traits.
Organisms mostly will be the same when they are in the same family, and if there are two organisms in different families that look alike, it will be a coincidence.
Answer: organisms in the same order
Yeet Skeet
Complexity is a measure of how difficult it is to understand something. What is an example of complexity in the natural world?
Answers
Answer:
Answer:
Multicellular organism is the example of complexity of the natural world.
Explanation:
Multicellular organism such as human which is made of billions of cell. Each cell perform a specific function. The function of one organ is different from the other organ. For example, brain of human is made of millions of neurons which takes instructions from brain to the organs and bring messages from organs to the nervous system in the form of electrical impulses. In short every system in our body is full of complexity.
1. Summarize the scientific information that leads to conservation in each of the articles.
2. What social issues affected the problem or its solution in each of the stories?
3. How did economics delay scientists' first attempts for conservation in each story?
4. Describe the political actions that led to successful conservation in both stories.
Answers
Answer:
Explanation:
BY USING FOREST WISELY;
It was learnt that people are cutting trees at an worrying rate.This problem is disturbing because ;
1)Those trees are responsible for releasing of oxygen and they made up at least a quarter of the world population.
2) Those trees of the world make up of portion land species by more than forth or fifth portion.
There are also economic implications as a result of trees been cut down, when there is no availability bot trees , it could result to situation whereby there would be scarce of resources for industries as well as
hike price of paper in market, regardless of this trees are needed for manufacturing of important materials such as usage in making houses and others developments or growth
Therefore, all the afformentioned economic concern result in economics delay scientists' first attempts.
COMMUNITY CONSERVATION;
It was learnt that there is to the forest that those gorilla habitated, those human being that reside in those region reside there so they can practice their farming because their is available land there. They also reside there because of housing.
These gorillas were affected as a result of the destruction made to their habitat as well as the activities of the poachers that hunt them for production of the and skin.
There are adverse effect of the obliteration of the gorilla from on the society, it can result to having reduction in tourism in country such as
Uganda as well as Rwanda, as result of this the means of livelihood of people in that part could be affected because there would be Reduction in profit making. Hence the reason behind the increase in number gorillas in the region, because they know they know that those gorilla influence the number of tourist that comes there and the revenue that is generated through this tourism.
The article 'By using Forest Wisely' can be summarised as:
People are cutting trees at the wrong rate and trees play a crucial role in an ecosystem. The trees produce oxygen and they made up at least a quarter of the world population.The trees occupy the fourth or fifth portion of the land organisms.In the absence of trees, the ecosystem will fail and the organisms dependent on them will eventually die. The resources available from the trees will be scarce and the factories and industries that are dependent on trees will no longer be available. Trees are required for manufacturing important materials.The article 'Community Conservation' can be summarised as:
In the article, it was mentioned that the human populace urbanized the land region where gorillas were habituating. The humans occupied the space to practice farming and make houses. The population of the gorillas was disturbed and diminished. The hunting of gorillas by poachers was also reduced. The people of Uganda and Rwanda also suffered reduction tourism and conservation of wildlife. Gorillas influence the number of tourists, therefore, the destruction of their habitats led to a reduction in profit-making.Therefore, all the mentioned economic concerns result in economics delaying scientists' first attempts.
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How is the epithelium suited for the substances ?
Answers
Answer:
Epithelial tissue is composed of cells laid together in sheets with the cells tightly connected to one another. Epithelial layers are avascular, but innervated.
Epithelial cells have two surfaces that differ in both structure and function.
The epithelial cells are nourished by substances diffusing from blood vessels in the underlying connective tissue. One side of the epithelial cell is oriented towards the surface of the tissue, body cavity, or external environment and the other surface is joined to a basement membrane. The basement layer is non-cellular in nature and helps to cement the epithelial tissue to the underlying structures.
The changing of the rivers created more ____________ for animals
aRegeneration
bVegetation
c Erosion
d Habitats
Answers
Answer:
D) Habitats for animals.
Explanation:
It depends what the question means when it says 'the changing of the rivers'. I took it as the rivers are changing size and becoming more spread out. In that case, I chose habitats.
The more water there is, the more habitat there is for sea life.
All in all, the correct answer is D).
All living organisms are composed of what?
Answers
All living organisms are composed of one or more cells.So, your answer would be Cell.
hope it helps!
2 Points
Which best describes the blood flowing in a vein?
O A. It moves toward the heart.
B. It contains no red blood cells.
O C. It is oxygen rich.
D. It contains no white blood cells.
Answers
Where is dna found
1. Molecules
2. Elements
3.cells
Answers
Answer:
3
Explanation:
Answer:
the dna is found in the cell nucleus
Explanation:
what is the role of a cytoplasm
Answers
Answer:
Cytoplasm is responsible for giving a cell its shape. It helps to fill out the cell and keeps organelles in their place
Explanation:
Cytoplasm contains molecules such as enzymes which are responsible for breaking down waste and also aid in metabolic activity.
what is the best definiton of genetically motified foods? HURRYYY!!! please and thank youuuu
Answers
Answer:
GMO, or genetically modified foods, are foods that are messed with genetically by science to better benefit the human race. For example, scientists might replace genes that give corn a lower harvest, with a different gene in corn that makes a higher amount of harvest.
Explanation:
You could also look it up. Just search "GMO".
Which action would be completed by skeletal muscle tissue 1.moving blood
2.increasing the heartbeat, or 3. kicking a soccer ball
Answers
Answer:
Kicking a soccer ball
Explanation:
Answer:
Kicking a soccer ball
Explanation:because moving blood and having a heartbeat arent in need of a skeletal system
Which is the result of an object's motion?.
Answers
Answer:
Your answer would be if any of the object increases in mass. (A)
Explanation:
If we increase the mass of any object, the force of gravity would also increases.
hope it helps!
Edit: Nevermind! I figured it out!!
Use the information in the table to calculate the ages of the meteorites. The half-life of potassium-40 is 1.3 billion years.
Meteorite Initial Amount(g) Final Amount(g)
1 30 7.5
2 80 5
3 100 12.5
Use your calculations to answer the questions.
How old is meteorite 1?
How old is meteorite 2?
How old is meteorite 3?
Answers
Answer:
meteorite 1- 2.6 billion
meteorite 2- 5.2 billion
meteorite 3- 3.9 billion
Answer:
meteorite 1- 2.6 billion
meteorite 2- 5.2 billion
meteorite 3- 3.9 billion
Explanation:
explain the difference between the principles of overload and progression
Answers
Describe natural selection
Answers
Answer:
Natural selection is the differential survival and reproduction of individuals due to differences in phenotype. It is a key mechanism of evolution, the change in the heritable traits characteristic of a population over generations.
Explanation:
Marijuana is a(n)
a. depressant
b. stimulant
c. hallucinogen
d. opiate
Answers
Answer:
Marijuana is a stimulant.
Answer: Marijuana is a stimulant.
Easy 50 Points
21. ____________ igneous rocks solidify beneath the surface.
22. ____________ cooling allows time for large crystals to form.
23. Sediments are laid down, or __________, before they can be formed into sedimentary rocks.
24. Fluids that crystallize in the spaces between the loose particles of sediments create rock by ____________.
25. __________ metamorphism changes enormous quantities of rock over a wide area.
26. ____________ metamorphism changes a rock that is in contact with magma because of the extreme heat.
27. In a metamorphic, minerals separate by __________ into lighter and darker bands.
28. The rocks that form from an erupting volcano are __________ __________ rocks.
29. Cemented sediments become ___________ sedimentary rocks.
30. ___________ occurs when sediments are squeezed together by the weight of overlying sediments on top of them.
Answers
Answer:
21. Intrusive igneous rocks solidify beneath the surface.
22. Slow cooling allows time for large crystals to form.
23. Sediments are laid down, or Compacted, before they can be formed into sedimentary rocks.
24. Fluids that crystallize in the spaces between the loose particles of sediments create rock by Cementation.
25. Regional metamorphism changes enormous quantities of rock over a wide area.
26. Contact metamorphism changes a rock that is in contact with magma because of the extreme heat.
27. In a metamorphic, minerals separate by Bedding into lighter and darker bands.
28. The rocks that form from an erupting volcano are Extrusive Igneous rocks.
29. Cemented sediments become Clastic sedimentary rocks.
30. Lithification occurs when sediments are squeezed together by the weight of overlying sediments on top of them.
The correct blanks are Intrusive, Slow, deposited, cementation, Regional, Contact, Foliation, volcanic, igneous, lithified, and Compaction.
Igneous rocks are a type of rock that forms from the solidification and crystallization of molten material, known as magma or lava.
Intrusive igneous rocks, also known as plutonic rocks, form when molten magma cools and solidifies beneath the Earth's surface.When molten magma or lava cools slowly, it provides sufficient time for the atoms and molecules within the molten material to arrange themselves in an orderly manner. The process of deposition refers to the settling or accumulation of sediments, which are fragments of rocks, minerals, organic matter, or other particles, onto a surface.Cementation is a process in which minerals in fluid form, often dissolved in groundwater, precipitate and crystallize within the pore spaces of sedimentary materials. During regional metamorphism, large volumes of rock are subjected to significant heat and pressure, typically deep within the Earth's crust. Contact metamorphism, also known as thermal metamorphism, occurs when a rock comes into direct contact with magma or an igneous intrusion.Foliation refers to the parallel alignment of minerals or the development of compositional layering within a metamorphic rock. Volcanic igneous rocks are classified as extrusive igneous rocks because they are formed from lava that has extruded onto the surface.The process of lithification involves the transformation of loose sediments into solid sedimentary rocks.Compaction is a key process in the lithification of sedimentary rocks. It takes place as layers of sediment accumulate over time, and the weight of the overlying sediments exerts pressure on the underlying layers.Thus, the correct blanks are Intrusive, Slow, deposited, cementation, Regional, Contact, Foliation, volcanic igneous, lithified, and Compaction.
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Which is the best evidence that two species have a common ancestor?
A:The two species have the same diet.
B:The two species live in the same habitat.
C:The two species' skeletal structures are 90% identical.
D:The two species' DNA sequences are 90% identical.
Answers
Answer:
I'd go with D.
Explanation:
Species may share similar physical features because the feature was present in a common ancestor (homologous structures). Molecular biology. DNA and the genetic code reflect the shared ancestry of life. DNA comparisons can show how related species are.
What is the best definition of a chromosome? Based on the picture provided above :)
Answers
Answer:
d.
Explanation:
A chromosome is a strand of DNA that is encoded with genes. In most cells, humans have 22 pairs of these chromosomes plus the two sex chromosomes (XX in females and XY in males) for a total of 46. The word chromosome was originally coined in German from the Greek words khroma, meaning color, and soma meaning body.
how did darwin’s theory of evolution change the way biologists thought about classification categories
Answers
Answer:
hhh3h3h3hgegegegegeggegsgsggs
y
Explanation:
hhhhhhshshshshhdhdhdhghdhgd
A 30 kg object has 3 forces acting on it - one 40 N force to the right, one 20 N force to the right, and one 30 N force to the left. What is the acceleration of the object?
A. 1.0 m/s2 to the right
B. 0 m/s2
C. 1.6 m/s2 to the right
D. 3 m/s2 to the left
Answers
Answer:
Thats not biology , that's physics . Net force = F1+F2-F3=40+20-30=60-30=30
F=ma.
30=30a
a=30/30
a=1m/s^2
. Why is the carpel considered female and the stamen male?
Answers
Answer: A carpel is the female reproductive part of the flower, interpreted as modified leaves that bear structures called ovules, inside which the egg cells ultimately form and composed of ovary, style and stigma. Stamen, the male reproductive part of a flower, consisting of a long slender stalk and the pollen-producing anther.
Explanation:
In ancient times, why would a cloudy day make it so hard to tell what time it is?
Answers
Answer:
Because celestial bodies such as the sun and stars were used to tell time, so if they were obstructed by clouds it would be hard to tell time
With more carbon in the atmosphere, what do you think will happen to the environment
Answers
Answer:
Global warming
Extra carbon dioxide in the atmosphere will increases the greenhouse effect. More thermal energy is trapped by the atmosphere, causing the planet to become warmer than it would be naturally. That increases the Earth's temperature which is also called Global Warming
Bacteria are usually classified by
Answers
Answer: Bacteria are classified into 5 groups according to their basic shapes: spherical (cocci), rod (bacilli), spiral (spirilla), comma (vibrios) or corkscrew (spirochaetes). They can exist as single cells, in pairs, chains or clusters. Artwork of bacterial cells becoming resistant to antibiotics.
Explanation:
Which type of organism developed first?
A. Chordates
B. Vertebrates
C. Deuterostomes
D. Sponges
Answers
Answer: D sponges, i just got this wrong lol
Explanation:
Why sponges develop first?
Sponges belong to the oldest and most simple animals that had evolved, and their discovery in such old rocks meant that they may have been the first animals.
How did sponges evolve?
Among extant animals, only sponges could have evolved directly from protozoa without changing feeding mode.
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