Protein phosphatase 1 (PP1) catalyzes a reaction which yields products that regulate cell division; consequently, PP1 is a possible drug target to treat certain types of cancers. The PP1 enzyme cata- lyzes the hydrolysis of a phosphate group from myelin basic protein (MBP). The reaction is shown below:
PP1 MBP-phosphate-→ MBP+P,
The activity of PP1 was measured in the presence and absence of the inhibitor phosphatidic acid (PA). The concentration of PA was 300 nM. vo without PA vo with PA released . (mg/mL) mL"1 . min1) mL-1 . min-1) 0.00381 0.00620 0.00931 0.0140 0.010 0.0209 0.0335 0.0419 0.0838 0.015 0.025 0.050
(a) Use the data provided to construct a Lineweaver-Burk plot for the PP1 enzyme in the presence and absence of PA. What kind of inhibitor is PA?
(b) Report the KM and Vmax values for PP1 in the presence and absence of the inhibitor.

Answers

Answer 1

Answer:

Explanation:8iikk


Related Questions

which term describes a pure substance that made up of only one type of atom

Answers

Answer:

Element

Explanation:

In chemistry, two substances are referred to as PURE SUBSTANCES. One of the pure substances is ELEMENT and the other is compound. An element is a pure substance that contains only one type of atom.

In an element, only one type of atom makes up the structure. For example, H2 (hydrogen) and O2 are examples of elements because they contain only hydrogen and oxygen atoms respectively

In your experiment, you found the following offspring only:Tall green: 626 offspring.Tall yellow: 313 offspring.Short green: 309 offspring.Stay with your original hypothesis/assumption, test it using chi2 test and analyze the result (whether you want to reject the hypothesis and why).(6p)

Answers

Complete question:

1. You found a new species of garden pea and have decided to repeat Mendel’s experiments. You have obtained two true-breeding pea plants that are tall (T) with yellow pods (g), and short (t) with green pods (G), respectively. Capital letters indicate dominant traits.

a. What is the genotype and phenotype of the F1 generation? (2p)

b. You allowed self-fertilization in F1 generation and obtained F2 generation. Using Punnett square to obtain the phenotypes, genotypes and their respective ratios. (4p)  

2. If you obtained 1248 offspring in the F2 generation in Question 1, calculate how many offspring of each phenotypic class you would expect to have. (4p)

3. When you finish questions 1 and 2, under what assumption/hypothesis did you perform your analysis/calculation? Is there an alternative assumption/hypothesis, which may result in a different conclusion? If yes, what is the alternative assumption/hypothesis and what kind of offspring and in what numbers do you expect to obtain? (4p)    

4. You only need to answer A or B to get this 6p. Please indicate which question you are answering. In your experiment, you found the following offspring only: Tall green: 626 offspring. Tall yellow: 313 offspring. Short green: 309 offspring.  

A: If you stay with your original hypothesis/assumption, test it using chi2 test and analyze the result (whether you want to reject the hypothesis and why).

B: If you believe that the alternative hypothesis fits better with your data, test it using chi2 test and analyze the result (whether you want to accept the hypothesis and why).

Answer:

1) a. 100% of the progeny will be dihybrid, TtGg, exhibiting the phenotype    

       Tall plants with green pods.

   b.  F2 Gentotype:

1/16 TTGG2/16 TTGg1/16 TTgg2/16 TtGG4/16 TtGg2/16 Ttgg1/16 ttGG2/16 ttGg1/6 ttgg

      F2 Phenotype:

9/16 Tall plants with green pods (T-G-)3/16 Tall plants with yellow pods (T-gg)3/16 Short plants with green pods (ttG-)1/16 Short plants with yellow pods (ttgg)

Phenotypic ratio → 9:3:3:1

2)  T-G- → 702 individuals

     T-gg → 234 individuals

     ttG- → 234 individuals

     ttgg → 78 individuals

3)

Null Hypothesis: The population is under Hardy-Weinberg equilibrium. The alleles of this population assort independently.Alternative Hypothesis: The population is not in equilibrium. Alleles do not assort independently.

4) a. There is enough evidence to reject the null hypothesis, meaning that the difference between the observed number of individuals and the expected ones is statistically significant. The population is not under equilibrium H-W. Alleles do not assort independently.  

Explanation:

1) 1st Cross: True-breeding tall (T) with yellow pods (g) pea plant with a short (t) with green pods (G) plant

Tall and Green pods are the dominant traits,Short and yellow pods are recessive traits.

Parentals)         TTgg         x         ttGG

Gametes) Tg, Tg, Tg, Tg          tG, tG, tG, tG

F1) 100% of the progeny will be dihybrid, TtGg, exhibiting the phenotype    

    Tall plants with green pods.

2nd Cross: F1 self-fertilization

Parentals)  TtGg     x     TtGg

Gametes) TG, Tg, tG, tg

                TG, Tg, tG, tg

Punnett square)    TG         Tg           tG          tg

                 TG      TTGG     TTGg      TtGG      TtGg

                 Tg       TTGg      TTgg      TtGg      Ttgg

                 tG        TtGG      TtGg       ttGG       ttGg

                  tg        TtGg       Ttgg       ttGg       ttgg

F2) Gentotype:

1/16 TTGG2/16 TTGg1/16 TTgg2/16 TtGG4/16 TtGg2/16 Ttgg1/16 ttGG2/16 ttGg1/6 ttgg

      Phenotype:

9/16 Tall plants with green pods (T-G-)3/16 Tall plants with yellow pods (T-gg)3/16 Short plants with green pods (ttG-)1/16 Short plants with yellow pods (ttgg)

Phenotypic ratio → 9:3:3:1

2) The total number of individuals in the F2 is 1248.

16 ----------------- 1248 individuals  --------------- 100% of the progeny

9 T-G- ----------- X = 702 individuals-------------X = 56.25%

3 T-gg ----------- X = 234 individuals ----------- X = 18.75%

3 ttG- ------------- X = 234 individuals ---------- X = 18.75%

1 ttgg ------------- X = 78 individuals ------------ X = 6.25%

3)

Hypothesis: The population is under Hardy-Weinberg equilibrium. The alleles of this population assort independently.Alternative Hypothesis: The population is not in equilibrium. Alleles do not assort independently

4)    F2 composed of:

Tall green: 626 offspring. Tall yellow: 313 offspring. Short green: 309 offspring.  

                       Tall/Green        Tall/Yellow         Short/Green       Short/Yellow

Observed              626                  313                        309                        0

Expected               702                  234                       234                       78

(Obs-Exp)²/Exp     8.23                  26.7                        24                        78

X² = Σ(Obs-Exp)²/Exp = 8.23 + 23.7 + 24 + 78 = 136.93 ≅ 137

Freedom degrees = genotypes - number of alleles = 9 - 4 = 5

Significance level, 5% = 0.05

Table value/ Critical value = 11.07

= 137

X² > Critica value

137 > 11.07

There is enough evidence to reject the null hypothesis, meaning that the difference between the observed number of individuals and the expected ones is statistically significant. The population is not under equilibrium H-W. Alleles do not assort independently.    

The upward force on an airplane's wing is thrust.


Please select the best answer from the choices provided

T
F

Answers

An airplane in flight is acted on by four forces: lift, the upward acting force; gravity, the downward acting force; thrust, the forward acting force; and drag, the backward acting force (also called wind resistance). Lift opposes gravity and thrust opposes drag .

Answer:

False

Explanation:

I am pretty sure that is the answer

Help due by 946 am pacific HELP ASAP

Answers

Answer:

What do you need help with? I see nothing attached.

What effect does planting trees and bushes on a steep hill have?



A- It makes the hill a good habitat for animals.

B- It provides oxygen to the soil.

C- Over time, it will help the hill grow taller.

D- It helps stop erosion.

Answers

Answer:

D- It helps stop erosion.

Explanation:

Erosion can be defined as a geological process which typically involves the wearing out of earthen (soil) materials and the transportation of these materials by natural forces like water, wind, etc. Soil erosion is greatest when the soil is steep.

The steepness of a body such as river or stream refers to the downward slope or gradient of the body of water.

Generally, the steepness of a body affects the rate at which other materials would flow or move around. Thus, the steeper a river or stream, the greater would be its rate of erosion. Other factors that affect the rate of erosion in an area include the following; farm management, rainfall, climate, soil type, soil cover, landscape, type of wave, crops, rock strength and position of coast.

In order to mitigate or stop the rate of erosion in an environment, trees and bushes should be planted on a steep hill because they will obstruct the free flow of water, as well as absorbing it.

explain the following type of respiration with diagram in toad:
1..Buccal respiration
2..Cutaneous respiration
3.. Pulmonary respiration​

Answers

Answer:

1.Buccal pumping is "breathing with one's cheeks": a method of ventilation used in respiration in which the animal moves the floor of its mouth in a rhythmic manner that is externally apparent.

2.Cutaneous respiration, or cutaneous gas exchange, is a form of respiration in which gas exchange occurs across the skin or outer integument of an organism rather than gills or lungs.

3.Pulmonary ventilation is commonly referred to as breathing. It is the process of air flowing into the lungs during inspiration (inhalation) and out of the lungs during expiration (exhalation.

Yan Lang po Sana makatulung

Several examples of antisense RNA regulating translation in bacterial cells have been discovered. Molecular geneticists have also used antisense RNA to artificially control transcription in both bacterial and eukaryotic genes. To inhibit the transcription of a bacterial gene with antisense RNA, what sequence might the antisense RNA contain

Answers

Answer:

An antisense sequence with complementary to the target mRNA encoded by the gene of interest

Explanation:

An antisense sequence is usually a short RNA sequence specifically designed in the laboratory to bind by complementary to the DNA/RNA sequence of interest (usually a target mRNA) whose expression is desired to be inhibited. When an antisense bind to its target mRNA, the antisense sequence acts to block translation, thereby inhibiting protein synthesis. Moreover, it has been shown that the double-stranded antisense-RNA complexes may also increase the susceptibility of the target sequence to RNase H digestion.

What's muscles are affected?PLEASE ANSWER a-c

Answers

Answer:

do it in your self hahahahahaa

6. A bus travels down a straight highway and goes from 0 to 10m/s in 5 seconds.

What is its acceleration?

a. 2m/s2

b. 3m/s2

c. 4m/s2

d. 5m/s2 7. A car can travel 50 meters in 10 seconds. A fast motor bike can travel 5 meters

in a second. A bus can cover 500 meters in 100 seconds. Which vehicle has the

highest speed?

a. The car has the highest speed because it travels a great distance in a

short time.

b. The bus has the highest speed because it can travel the greatest distance.

c. The bike has the highest speed because it has the shortest travel time.

d. All their speeds are equal.

8. Which of the following is TRUE about speed and velocity?

a. Speed is a vector and velocity is scalar.

b. Velocity is the speed in a particular direction.

c. An object can accelerate even if the velocity is constant.

d. A change in speed causes an acceleration. A change in velocity causes

deceleration.

9. A bicycle has an average speed of 2m/s. This means that _________.

a. in 1 second, it travels 1 meter

b. it can travel 4 meters in 2 seconds

c. it takes 2 seconds to travel 1 meter

d. it can travel 2 kilometers in one hour

10.An object can accelerate while moving at constant speed. Which situation

illustrates this?

a. A roller coaster making a fast dive.

b. A bus going uphill at constant speed.

c. A bump car going in circles at constant speed.

d. A car moving down a straight highway at constant speed.​

Answers

Answer:

6) 2m/s²

7)All the speeds are equal

8) Velocity is speed in a particular direction

9) it can travel 4 m in 2 sec

10)a bus going uphill at constant speed (not sure abt this)

How do community contribute in maintaning the health of ecosystem??

Answers

Answer:

THE MATEMÁTICAS 163 por eso

Which of the following is a true statement? Physical property change is always better than chemical property change.
o Physical and chemical propery change can happen at the same time.
Chemical property change is better than physical property change.​

Answers

Answer:

the second one

Explanation:

Sometimes it can be tricky to know whether or not a chemical reaction has occurred. For example, when you melt ice into water, you can write the process in terms of a chemical reaction. However, the chemical formula on both sides of the reaction is the same. Since the chemical identity of the matter in question is unchanged, this process represents a physical change.

Thus melting point is a physical property. On the other hand, flammability is a chemical property of matter because the only way to know how readily a substance ignites is to burn it. In the chemical reaction for combustion, the reactants and products are different.

none are better, depending on for what

Which statement describes the role of decomposers in moving nutrients through a food web?

Answers

Answer:

Every part of an ecosystem is vital to its survival – from the green plants to furry animals and microscopic bacteria. The group of organisms called decomposers forms the final link in the food chain. They break down dead animals and plants and return vital nutrients to the soil.

Explanation:

20. Which does NOT describe primary succession? *
O Decomposing organic matter forms soil
O Presence of existing fertile soil
O Long-term occurrence over extended time
Barren land once covered by glacier

Answers

i think it is B because primary succession is something that can grow on rocks and exposed land. the answer is B because primary success doesn’t include soil where soil is incapable of sustaining life

DNA sequence that codes for glycine​

Answers

qwertyuiopal!!!! :))))

What is the purpose of cellular respiration?

a
break down sugars to use their energy directly
b
capture energy from the sun and store it in sugars
c
break down sugars to create ATP
d
capture energy from the sun and store it in sugars

Answers

A. Break down sugars to use their energy directly

Anorexics are often hungry but refuse to eat, which is misleading because the literal meaning of anorexia is “__________.” A. unhealthy dieting B. excessive hunger C. excessive weight loss D. loss of appetite

Answers

Answer: D

Explanation:

Answer:

D edge 2021

Explanation:

If a very massive object is stationary its momentum is what

Answers

Action and reaction forces, in contrast, act on different objects, so they don’t cancel out. In fact, they often result in motion.

50. The flow chart below shows how a metamorphic rock can change into a

sedimentary rock. Which of these processes would be the correct label to

replace the question mark?

Metamorphic rock

Sediment

Sedimentary rock

Erosion

?

A) Cooling

B) Deposition

C) Melting

D) weathering

Answers

Answer:

The process that would be the correct label to replace the question mark in this question is:

B) Deposition

Explanation:

Deposition takes place after erosion of sedimentary rock.  At this stage, the eroded sedimentary rocks build up under high pressure heat, wind, ice, water, and other natural forces to originate the rock formation cycle once again.  Naturally, after deposition, the igneous rocks, which have then layered up sedimentary rocks to become metamorphic rocks.

(3)
2.1
2.2
23
Provide labels for structures A, B and
State THREE functions of D
Fertilisation usually takes place at Y. Why wil
a blockage at X
2.3.1 Prevent fertilisation at Y
2.3.2 Not necessarily lead to infertity
(2)
3. Study the graph below which shows the menstrual
cycle and the influence of the different hormones on
it.​

Answers

Answer:

i am pro i am pro i am pro i am pro

In all the laboratories the benches are placed at right angles to windows. Why

Answers

Answer:

in case of emergency

Explanation:

How many proton , neutrons , and electrons are there barium ?

Answers

Answer:

137.327 atomic mass units

hope this helps

Explanation:

Answer:

56 protons, 81 neutrons and 56 electrons

Explanation:

hope that helps

Two parents have three kids. Each kid looks very different physically. What is responsible for this outcome?

Answers

Answer: This may mean that the mother has been seeing people and the kids don't have the same dad or They don't really have a lot of their parent's genes.

Explanation:

really the only options I can think of.

Can we depend on nonrenewable energy resources for our energy needs?

Answers

Answer:by

Explanation:

Answer:

Explanation: The answer is yes we can. We are depending on them now and they supply most of our needs to live.

Hope this helps and feel free to copy and paste it :)

Atoms of which two elements have a combined total of 23 protons?


Boron, B, and carbon, C



Sodium, Na, and magnesium, Mg



Copper, Cu, and zinc, Zn



None of these

Answers

Answer is sodium, Na, and magnesium, Mg

Help me please!!!!!!!!!!!!!!!!!j

Answers

Answer:

50%

Explanation:

The rotation of the earth causes winds to shift and ocean currents to change. This is an example of?
A) inertia.
B) cyclones.
C) acceleration.
D) topographical variation

Answers

Answer:

d) topographical variation

Explanation:


Do you think the amount of foam in the glasses will differ?

Answers

The amount of foam in the glasses will differ. In terms of increasing foam rise, Glass A will be the least, then E, D, B, and C having the most foam rise. ... This is being tested from the carbon dioxide output of the glass which is measured from the height of the foam.

Answer:

these are all the answers to the entire course activity

Please mark as brainliest if this helps

Explanation:

13. Which of the following shows the correct relationship between warm
ocean water and its movement? *

Answers

Answer: B

Explanation:

The following that shows the correct relationship between warm  ocean water and its movement is:

A. The density increases and the movement rises

Warm ocean water is the condition in which the temperature of the ocean is warm. This in turn affects climatic conditions as it also has an effect on the marine ecosystem.

       

As a result of the temperature, warm water usually stays at the horizon of the ocean and when there is a joining of the cold water and the warm water, then there is a change in density as it increases while the movement of the water rises.

This phenomena occurs as a result of convection and the movement changes because warm water is less dense. The Indian Ocean is the warmest ocean in the world.

Therefore, the correct answer is option A

Read more here:

https://brainly.com/question/21059521

Why the Carbon must be cycled through the carbon cycle?

Answers

Answer:

The carbon cycle describes the process in which carbon atoms continually travel from the atmosphere to the Earth and then back into the atmosphere. Since our planet and its atmosphere form a closed environment, the amount of carbon in this system does not change.

In the citric acid cycle when an aldol addition occurs in a biological system, how do the amino acids contained in the Citrate Synthase enzyme help facilitate the reaction

Answers

Answer:

Citrate Synthase facilitates the formation of citrate by deprotonating Acetyl CoA and by protonating the carbonyl oxygen of oxaloacetate

Explanation:

Citrate synthase is a key enzyme in the citric acid cycle that functions to catalyze the formation of citrate from oxaloacetate and acetyl-CoA: acetyl-CoA + oxaloacetate + H2O >> citrate + CoA-SH (Coenzyme A ). This enzyme contains three amino acids at its active site that work together to catalyze the conversion of acetyl-CoA and oxaloacetate into citrate. This active site acts by abstracting (deprotonating) a proton from the alpha carbon of Acetyl CoA which serves as the nucleophile. Subsequently, the active site of the enzyme also protonates the carbonyl oxygen of oxaloacetate, which then suffers nucleophilic attack and thereby facilitating nucleophilic substitution reaction.

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