Proteins are important food nutrients. A technician working at a food company is interested in the nutritional content of seeds/nuts. She runs a PAGE with samples from two different seed extracts. She runs multiple lanes of the staples. The gel is stained with Coomassie Blue. Seed extract No. 1 has three faint bands at 25, 30, and 35 kDa. Extract No. 2 has two bands at 25 and 35 kDa, and the bands are very dark. What might she conclude from her results about the nutritional value of the seed extracts

Answers

Answer 1

Answer: Sample 1 then, has more variety of proteins but less quantity of each one, while sample 2 has less variety but more quantity of the two proteins present.

Explanation:

Proteins are molecules found in the cells of all living organisms. A protein is composed of monomers called amino acids linked together by peptide bonds. To analyze proteins, a technique called SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis) is used. This technique is used to separate proteins according to their electrophoretic mobility, depending on polypeptide chain length, molecular mass, post-translational modifications and other factors. SDS (sodium dodecylsulfate) is an anionic surfactant compound that breaks non-covalent bonds in proteins, denatures them causing these protein molecules to lose their native conformation and provides the polypeptide with a negative charge that is proportional to the chain length (the number of amino acids) and, therefore, to the molecular mass of the protein. The electrostatic repulsion created by the binding of SDS to the protein is one of the causes of the protein losing its native conformation, thus eliminating the differences in conformation of the different proteins to be separated in the gel.

An electric current is applied to the gel where the proteins are seeded, causing the negatively charged proteins to migrate through the gel in the direction of the anode (positively charged region). Short proteins will move more easily and faster through the pores of the gel, while larger proteins will find it more difficult to do so. After a certain time, the proteins will have migrated according to their size (measured in kDa or kiloDaltons). After electrophoresis, the gel should be stained with Coomassie blue or silver stain, allowing visualization of the separated proteins. After staining, the different proteins will appear as distinct bands on the gel. In addition, the higher the amount of a protein, the more intense the color of its band will be.

Extract No. 1 shows three bands: 25, 30 and 35 kDa. This means that it has three different proteins, each with a different molecular weight. Extract number 2 shows two bands: 25 and 35 kDa. It means that this sample has only 2 proteins. And being bands of higher intensity, we can say that there is more of each protein.

Sample 1 then, has more variety of proteins but less quantity of each one, while sample 2 has less variety but more quantity of the two proteins present.


Related Questions

A single-celled organism can be classified as a prokaryote based on the absence of-

Answers

Answer:

A nucleus and membrane bound organelles.

Answer:

lack of nucleus.

Explanation:

A prokaryote is a simple, single-celled organism that lacks a nucleus and membrane-bound organelles.

Please Help.

What is the name of the process that plants use to remove carbon dioxide from the atmosphere?

1.) transpiration
2.) photosynthesis
3.) respiration
4.) decomposition

Answers

Photosynthesis is the answer

True or False: The most fertile agricultural land in North America is used to produce mostly wheat and corn.

Answers

True

I looked up the most fertile land in the US (Great plain region)

Then I looked at a crop map

Looks like it’s mostly wheat and corn grown in the Great Plains

Hope this helps.

what are the different ways to recycle biodegradable waste?​

Answers

Answer: Composition maybe?

Explanation:

Question 4 of 10
What is the main function of the circulatory system?
O A. To break down food into molecules of nutrients
B. To collect wastes made by cells and remove them from the body
O c. To carry nutrients to cells and wastes away from cells
O D. To protect the body from pathogens that can cause infection
SUBMIT

Answers

The answerrrrr is B :)(

Predict the type of relationship between the ant and the ant-lion.
A. Commensalistic

B. Mutualistic

C. Predator-Prey

D. Parasitic

Answers

Answer:

C. Predator-Prey

Explanation:

There are predator-prey relationship between ants and antlions.

The type of relationship between the ant and ant lion is Predator-Prey. The correct option is C.

What is predator-prey relationship?

Predator-prey relationship implies to the interactions among the two species where one species is the killed food resource for the another one.

The organism that feeds is referred to as the predator while the organism that is fed upon is basically the prey. There are mainly most variety of illustrations of predator-prey relations.

Competition as well as predation are ecological interactions but are not symbiotic.

Predation usually does not take place over a long period of time, along with the competition is an indirect interaction on other resources.

Predation is a symbiotic interaction in which one species sustains life on costuming the another species.

The species that win is the predator, and the species that is lost up is the prey.

Thus, the correct option is C.

For more details regarding predator-prey relationship, visit:

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what is the process of transformation?
infecting bacteria with bacteriophages
adding foreign DNA to a bacterial cell
copying foreign DNA into RNA
causing mutations in viral genetic material

Answers

is this all in the same question?

Individual bones are organs.
A. True

B. False

Answers

Answer:

a

Explanation:

Answer:

True

Explanation:

Because they are made out of tissues

12. Flowers whose reproductive structures consist only
of stamens would be able to produce
A) fruits with seeds
B) fruits without seeds
C) pollen
D) ovules

Answers

Answer:

Pollen reproductive structures consist only

of stamens would be able to produce

Explanation:

No C is the answer

Reproductive structures are structures that make up the reproductive organs of a particular organism. These structures could include either female or the male reproductive organs.

Flowers with reproductive structures that comprise of only stamen would produce only pollen (option c).

Stamens are reproductive organs found in plants and they are male reproductive organs. For fruits with seeds to be formed the male and female reproductive organs must come together to produce a fruit.

Flowers with only the stamen will produce only pollen which is a male gamete that needs to be joined to the female gamete, ovary produced from the female reproductive structure (pistil) in a plant to produce a fruit.

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A mammal's red blood cells produce the protein hemoglobin, which carries oxygen through the bloodstream. Its hair follicle cells do not produce hemoglobin. Which statement is true about these differences? A) The red blood cells contain different genes than the hair follicle cells, causing the cells to differentiate B) The red blood cells contain DNA, but the hair follicle cells do not C) A red blood cell and hair follicle cell have the same genes, but they are expressed differently D) The hemoglobin is produced from recombinant DNA, which is only found in red blood cells

Answers

Answer:

C) A red blood cell and hair follicle cell have the same genes, but they are expressed differently

Explanation:

All cells of a living organism contain the same genetic sequence i.e. they contain the same set of genes, however, differences in the expression of these genes may occur. This is responsible for the different specific roles or functions played by organs of the body system.

The expression of a gene refers to the process by which a gene is transcribed and translated into a protein molecule, which is responsible for traits in the body. Genes are expressed differently in order for certain cells to function differently and specifically. For example, difference in expression of genes is responsible for the ability of red blood cells to produce hemoglobin and the inability of hair follicle cells to, even if they contain same genes.

A functional or physiological adaptation:
A. Is a physical feature of an organism that helps it survive

B. Allows an organism to perform special functions within the body

C. Is a behavior that is taught after birth

Answers

Answer:

A trust me.

Explanation:

are you functional adaption is an adaption that helps an organism survive in a certain environment, typically altered by a physical stimuli, dietary changes, or injury.

Answer:

yes behavior is taught after birth

Me pueden ayudar con la respuesta? Es urgente!

Answers

A: especie

B: gen

C: desarollo

D:

E:deriva genetica

F:población

G:mutuación

no ps no pude con la D-

- The ability of an ecosystem to maintain a steady equilibrium by responding to
internal and external negative feedback systems describes -

Answers

Answer:

Describes ecosystem homeostasis

Explanation:

The light reactions can only occur in the absence of light.
A. True

B. False

Answers

Answer:

false

Explanation:

the light reactant needs light

2. Animal Nutrition
Question 1
Give one suitable biological term for each of the following
1. Muscular contractions in the intestine causing the movement of food
2. Food that has a high fibre content.
3. A nutritional disease caused by a person refusing to eat.
4. Physical breaking down of food in the mouth by the teeth
5. Finger-like projections in the small intestine
6. The breaking down of large fat molecules into tiny droplets.
7. Process by which the liver converts excess amino acids into urea.
8. Region of the alimentary canal where most of the water is reabsorbed.
9. Muscular organ connecting the pharynx to the stomach.
10. Valve that controls the entry of food from the stomach into the duodenum,

Answers

1. Muscular contractions in the intestine causing the movement of food.

Peristaltic movement.

2. Food that has a high fibre content.

Fibrous

3. A nutritional disease caused by a person refusing to eat.

Malnutrition

4. Physical breaking down of food in the mouth by the teeth

Mastication

5. Finger-like projections in the small intestine

Villi

6. The breaking down of large fat molecules into tiny droplets.

Emulsification

7. Process by which the liver converts excess amino acids into urea.

Excretion

8. Region of the alimentary canal where most of the water is reabsorbed.

Large intestine

9. Muscular organ connecting the pharynx to the stomach.

Esophagus or Oesophagus

10. Valve that controls the entry of food from the stomach into the duodenum,

Pyloric sphincter

Why do scientists only test one independent variable in an experiment?

Answers

To not getting confusing results. By isolating one variable scientists can conclude the results of the experiment were directly impacted by the variable they changed and nothing else.
Testing only one variable at a time helps you look at the results of your experiment and to see how much a single change affected the result.

Which problem-solving technique involves drawing conclusions from a few observations?
A.
drill-down technique
B.
inductive reasoning
C.
constructive controversy
D.
reverse brainstorming

Answers

Answer: B.

inductive reasoning

Explanation:

Inductive reasoning, or inductive logic, is a type of reasoning that involves drawing a general conclusion from a set of specific observations. Some people think of inductive reasoning as “bottom-up” logic, because it involves widening specific premises out into broader generalizations.

Hope it helps if not sorry

Item 5 What is the role of a generator in producing electricity? The generator stores electrical energy for later use in homes and businesses. The generator spins the turbine to create mechanical energy. The generator transforms the mechanical energy of the turbine into electrical energy. The generator reverses the turbine to create electrical energy.

Answers

Answer:

The generator transforms the mechanical energy of the turbine into electrical energy

Explanation:

Electricity generator is a device that is used in generating power supply which can be used in industries, homes and for commercial and private purposes.

It operates by converting mechanical energy into electrical energy for the use in an external circuit. mechanical energy can be obtained from steam turbines, internal combusting energy, gas and water turbines and it can be obtained through the rotating shaft.

The capacity of each generator determines the amount of energy it can supply to a given place.

help with the graph

Answers

if you had it in Spanish

Salad vegetables are cut and placed in plain water. Why?

Answers

Answer:

Not only does soaking produce rid it of harmful germs or chemicals, but it can also be used to revitalize produce for a better taste and longer life. If you purchase local, organic fruits and vegetables that contain no chemicals or harmful preservatives, you can use warm water to rinse your produce.

Explanation:

Fun facts:

Through osmosis, water naturally moves from areas of low salt concentrations to areas of high salt concentrations. ... Consequently, the water in a leaf of lettuce that is soaking in salt water will migrate out of the lettuce leaving a mushy leaf behind.

The chemical substances in our environment to which we respond can be called?
A. stimuli
B.impulses
C.responses
D.sense cells

Answers

A. Stimuli

WHAT IS STIMULI?

A stimuli is anything that can trigger a physical or behavioral change.The changes in the environment to which living things respond are called stimuli. The living things show response to external stimuli such as heat, light , touch, sound, smell, taste, water and chemicals.It can be internal or external both.When diverse organs works collectively in a scientific way to supply a reaction to the stimulus, this is known as coordination.Stimuli is essentially anything that triggers a receptor for one of those senses.

You can understand this with an example that would be the receptors on our skin which sense a stimulus like pain. Sense organs contain groups of receptors that respond to specific stimuli.

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How do electronegativity, polarity, and ph all relate and effect each other? I know moderators, you will be reported if you give a link or wasteful answer.

Answers

Answer:Electrons in a polar covalent bond are shifted toward the more electronegative atom; thus, the more electronegative atom is the one with the partial negative charge. The greater the difference in electronegativity, the more polarized the electron distribution and the larger the partial charges of the atoms.

Explanation:

ARTS :


true. or. false. ​

Answers

Answer:

true false true false true

Which Ali combination represents a male who has an X-linked recessive disorder

Answers

XrY is the Ali combination that represents a male who has an X-linked recessive disorder

A population of beach mice have been
introduced to a new disease. This
disease has killed off most of the
population. Some mice in the
population were better suited and
survived the disease, living to
reproduce. The population over time
has grown resistant to the disease.
This is an example of?
(1 Point)
*
Artificial Selection
Natural Selection
Selective Breeding

Answers

It is natural selection. Try to remember from those three selections that natural is where the animal does it by itself. The other two are where humans interfere with it.

All of living and noliving things in a particular area

Answers

What are we supposed to answer? There is no question?
That is the definition of an ecosystem

PLS HURRY AND NO LINKS PLEASE
If a long tail is a dominant characteristic, in which case is the characteristic of a long tail definitely expressed?
A when both parents have a long tail
B when neither allele contains instructions for a long tail
C when at least one parent has a long tail
D when at least one allele contains instructions for a long tail

Answers

Answer:

b

Explanation:

Answer:

the correct answer is d i took the test.

Explanation:


Which is an example of an ecosystem change that resulted from human activity?

Answers

Answer:

Your welcome! :)

Explanation:

An example is:

Deforestation occurs when a forest or stand of trees is removed, converting the land to a nonforest use. This changes the ecosystem drastically and results in a dramatic loss of biodiversity.

♡♡♡

Answer:

chaparral shrublands turning to grass due to frequent fires

Explanation:

I just took the quiz and it's right

Any reaction that is not intended is called

Answers

the free encyclopedia. A paradoxical reaction or paradoxical effect is an effect of a chemical substance, typically a medical drug, that is opposite to what would usually be expected. An example of a paradoxical reaction is pain caused by a pain relief medication.

27. Which statement best describes a chromosome?
A) It is a gene that has thousands of different
forms.
B) It has genetic information for traits of an
organism that is contained in DNA.
C) It is a reproductive cell that influences more
than one trait.
D) It contains hundreds of genetically identical
DNA molecules for one trait.

Answers

Answer:

B

Explanation:

Chromosomes are structures composed of DNA that, in turn, carry the genes of a living being, responsible for defining the physical characteristics of each individual. Chromosomes are involved in the nucleus of cells that make up a living being.

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two roads diverged in a yellow wood.and sorry i couldn't travel both.and be one travelled long i stood.and looked down one as far as i could. to where it bent in the undergrowth. summary Parrot eats only ........ products Which inequality represents the compound inequality x > 3 and x < 5 Solve the inequality for s:5s + 2 > 7Answer: 100 POINTS HELP! THIS IS A PROJECT. NOT IN A RUSH.Describe how to find the terms mean, median, mode, and range of a group of numbers. Pretend you are a teacher trying to describe these terms to a student. Make sure you write as if you are the teacher and explain each term as if the student is learning this for the first time. Be descriptive and give examples. Make sure you include the data set (the group of numbers) you will be working with. Be sure to use complete sentences. Be creative! What is the smallest counting number that is divisible by 2, 4, 5, 6, and 12? express each number and your final answer as a product of primes. What do you notice? What are some of the immediate results of the battlefields civil war? altogether i have 10 bags of sweets. the mean number of sweets in the bag is 40 pleaseee help me with this questionnn Mikey Johnson shipped out 41 1/4 pounds of electrical supplies. The supplies are placed in individual packets that weigh 2 3/4 pounds each . How many packets did he ship out? A bag contains 8 red balls and 6 blueballs.Radhika takes three balls at randomfrom the bag, without replacement.Calculate the probability that the threeballs are the same colour. * The Sneed Corporation issues 10,000 shares of $50 par preferred stock for cash at $75 per share. The entry to record the transaction will consist of a debit to Cash for $750,000 and a credit or credits to a.Paid-In Capital from Preferred Stock for $750,000 b.Preferred Stock for $500,000 and Retained Earnings for $250,000 c.Preferred Stock for $500,000 and Paid-In Capital in Excess of ParPreferred Stock for $250,000 d.Preferred Stock for $750,000 Caractersticas texto no literario PLEASE HELP WHAT ARE LEGIONNAIRES You are an investment manager and one of your clients is a famous soccer player. She is promised to be paid $5million three years from now. What is the present value of this cash flow today Question 12What could be an explanation if Juanito crosses one white flower with a red flower but allthe offspring are pink flowers?sex-linkedsimple Mendelian inheritancecodominancerecessive epistasisincomplete dominance The last leader of the Soviet Union was _____.Mikhail GorbachevLeonid BrezhnevNikita KhrushchevAndrei Sakharov What is the volume of this triangular prism? A triangular prism. The triangular base has a base of 28 centimeters and height of 22.4 centimeters. The height of the prism is 18.1 centimeters. 313.6 506.8 5,676.16 11,352.32 what animal has no heart?? how do you do this?