the dna with the gene of interest for a cloning experiment was left on the lab bench overnight (instead of storing it in the freezer). as a result, it was degraded. they didn't know the dna was left out and used it in the experiment. the plasmid, however, was stored correctly. the vector contains a gene for ampicillin resistance. after the transformation procedure, the bacteria were plated on media containing ampicillin. what results are expected from this faulty molecular cloning experiment?

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Answer 1

In a molecular cloning procedure, the DNA to be copied is extracted from a target organism and split into smaller DNA fragments in a test tube using enzymes.

These pieces are subsequently joined with vector DNA to produce recombinant DNA molecules. After that, a host organism is given the recombinant DNA (typically an easy-to-grow,strain of E. coli bacteria). Recombinant DNA molecules will be duplicated alongside the host DNA in the resulting population of organisms. These are transgenic or genetically modified organisms because they have foreign DNA pieces. Thus, the term "clone" is frequently used to describe both the bacterial population and the recombinant DNA molecule. In actuality, molecular cloning refers to the scientific techniques utilised to put them together.The idea arose that different DNA sequences could be inserted into a plasmid and that these foreign sequences would be carried into bacteria and digested as part of the plasmid. That is, these plasmids could serve as cloning vectors to carry genes.

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Related Questions

Which of the following is a characteristic of offspring produced by meiosis, and does not characterize offspring produced by mitosis?

A.
four haploid cells with chromosomes and alleles that are genetically different than the parents

B.
four haploid cells with chromosomes and alleles that are genetically identical to the parents

C.
two diploid cells with chromosomes and alleles that that are genetically different than the parents

D.
two diploid cells with chromosomes and alleles that are genetically identical to the parents

Answers

Answer:

the answer is B I did this last week

Why are the differences between prokaryotic and eukaryotic translation of mRNA useful to humans?

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For the regulation of genes during nutritional shortage and stress, development and differentiation, nervous system function, aging, and illness, translational control in eukaryotic cells is essential. Prokaryotes are able to respond quickly to environmental cues because transcription and translation happen simultaneously in the cytoplasm.

what are Prokaryotes ?

Any organism without internal membranes is referred to as a prokaryote, usually written procaryote. These organisms lack a defined nucleus and other organelles.

what is eukaryotic ?

eukaryotic can be explained as a living things with nuclei in their cells. Eukaryotes include all animals, all plants, all fungi, and many unicellular creatures.

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Explain why the potential energy increases when ice starts to melt and becomes liquid water.
Enter your answer in the space below.
swer inside this box

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Answer:

Explanation:

While the ice melts kinetic energy is being added to the particles. This breaks the bond and causes a change of state making the solid become a liquid.

sexual selection in a population choose one: a. can lead to physical differences between the males and females of a species. b. does not lead to evolution. c. reduces genetic variation in the population. d. always favors individuals with a higher survival potential.

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The right response is option a, which states that morphological distinctions between males and females of a species can result from sexual selection in a population.

The tail of a peacock is a good illustration of partner selection. Peahens choose peacocks with huge, vibrant tails, which allows those peacocks to mate more frequently and produce younger ones. The genes for a long, colourful tail will be passed down from the father to the male peachicks, who will inherit these genes and develop comparable tails. There are two main factors that influence sexual selection. One is the preference of one sex (typically females) for members of the opposite sex who display particular features. The other is higher strength, which (often in males) results in better success in courting females.

So, we can conclude that option a, which claims that sexual selection can lead to physical differences between males and females of a species in a population, is the appropriate response.

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Select all true statements regarding gamma decay.

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Gamma rays are the spectral particles with the highest energy and shortest wavelength. This form of decay does not result in the release of any particles from the nucleus.

What is a nucleus, and what does it do?

The spindle holds the alleles, which are the structures that house the genetic traits, and governs and regulates the functions of the cell. The nucleoplasm is the gel-like membrane that contains the nuclear parts are suspended.

Why is the nucleus the most significant cell?

Due to its role in the storage, retrieval, and copying of genetic material, the nucleus is regarded as among the most significant components of eukaryotic cells. The genetic material is housed in an organelle with two membranes.

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