The kinetics analyses of an enzyme are measured as a function of substrate concentration in the presence and in the absence of 2mM inhibitor

[S] (microM): 3, 5, 10, 30, 90

Velocity (micromol/min) No Inhibitor: 10.4, 14.5, 22.5, 33.8, 40.5
Velocity (micromol/min) With Inhibitor: 4.1, 6.4, 11.3, 22.6, 33.8

Required:
What are the values of Vmax and Km in the absence of inhibitor? In its presence?

Answers

Answer 1

Answer:

take the reciprocal of the values as 1/S and 1/V and plot the values on lineweaver-burk plot.

so the re-arranged data is as following

1/S               1/V (no inhibitor)       1/V (with inhibitor)

0.333           0.096                       0.244

0.2              0.068                         0.156

0.1               0.044                         0.088

0.033          0.029                        0.044

0.011            0.024                         0.029

and the plotted lineweaver-burk plot with the equation of straight line would be....

where, first line represents the velocity of reaction with no inhibitor and second line represent the velocity in presence of inhibitor. X-axis has the substrate concentration as 1/S and y-axis has the velocity as 1/V.

as per the plot, equation of line on the lineweaver-burk plot, Y = mx + c, where c = 1/Vmax and m = Km/Vmax.

so solve for the given equations.

First, equation of line in the absence of inhibitor is Y = 0.23x + 0.021

so Vmax = 1/0.021 = 47.6 micromol/min

and Km/Vmax = 0.23, or Km = 0.23 x 47.6

Km = 10.95

Km do not have a unit, so 10.95 x 10-6 or as per your values round it up to 1 decimal point as 1.1 x 10-5

so value of Vmax = 47.6micromol/min and Km = 1.1 x 10-5 in the absence of inhibitor.

similarly, equation of line in the presence of inhibitor is given as Y = 0.67x + 0.021

so Vmax = 1/0.021 = 47.6 micromol/min

and Km/Vmax = 0.67

or Km = 0.67 x Vmax = 0.67 x 47.6 = 31.89

Km do not have a unit, so 31.89 x 10-6 or 3.1 x 10-5

so value of Vmax = 47.6micromol/min and Km = 3.1 x 10-5 in the absence of inhibitor.


Related Questions

how does replication ensure each cell has a complete set of DNA

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Answer:

During replication a new strand of DNA is synthesized when the other strand is a template to guide the process. Every time the order of the bases in preserved so that DNA can be accurately replicated over and over with identical genetic information.

explain the concept of mutation and how mutations can translate to a loss or gain of function in an organism

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A mutation is a change in a DNA sequence. Mutations can result from DNA copying mistakes made during cell division, exposure to ionizing radiation, exposure to chemicals called mutagens, or infection by viruses. Germ line mutations occur in the eggs and sperm and can be passed on to offspring, while somatic mutations occur in body cells and are not passed on.

Recessive mutations inactivate the affected gene and lead to a loss of function. For instance, recessive mutations may remove part of or all the gene from the chromosome, disrupt expression of the gene, or alter the structure of the encoded protein, thereby altering its function.

how does Fibrocartilage receive it's nutrients?

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Answer:

There are no blood vessels in cartilage. As a result, the nourishment of cells inside the cartilage matrix is reliant on nutrients diffused through the matrix from blood capillaries in the perchondrium and/or surrounding tissues.

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What is the correct order of the levels of organization in animals from smallest to largest?​

Answers

Answer:

Molecules, cells, tissue, organ, organ system, organism, population, community, ecosystem, and biosphere are in the correct order of the organization's levels.

Explanation:

Molecule: Atoms, the smallest unit of chemical elements are built up of molecules. You can find it in any matter, whether it lives or not. The most fundamental structures of biological creatures are molecules. Biochemistry and molecular biology are two biological fields focused on this level.

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In most cases of multiple myeloma, the excess antibodies found in the blood all share an identical protein sequence (thus they have the same structure). Consider such a case where all of the antibodies are identical and think about the cell(s) of origin. How many different B cell clones gave rise to this immune cancer?

Answers

Answer: One single B cell clone gave rise to this immune cancer.

Explanation:

The body's cells divide and grow in a completely regulated manner, following an orderly cell cycle that indicates when a cell should die by apoptosis. With this regulation, it is controlled that there are no aging or mutated cells that grow and affect the organism. Cancer is a genetic disease caused by changes in these genes that control how our cells function. These changes, genetic mutations, can occur randomly in the body or can be inherited from parents. When a cell has its functions altered, they begin to grow out of control and in doing so, form masses called tumors that consume the body's resources and energy and crowd out normal cells, preventing them from fulfilling the biological functions necessary for life. Practically, any cell can become a cancer cell and migrate to other areas of the body.

Multiple myeloma is a type of plasma cell cancer. These cells are found in the bone marrow (tissue inside the bones) and are an important component of the immune system, which is made up of several types of cells that work together to fight pathogens and disease. Some cell types are B and T lymphocytes, and myeloid cells such as macrophages, dendritic cells, etc. When B cells respond to an infection with the participation of T cells that present pathogen-derived antigens to them, they mature into plasma cells. Plasma cells produce antibodies that help the body attack and destroy pathogens as well as cancer cells. These antibodies are proteins that bind to antigens that are also proteins found on the surface of the pathogens or cells to be attacked and "mark" them for destruction by other types of immune cells, such as macrophages or neutrophils.

When the plasma cells become cancerous and grow out of control, this is called multiple myeloma, and the mutated plasma cells produce abnormal antibodies. The overgrowth of plasma cells in the bone marrow can crowd out normal blood cell-producing cells, causing low blood cell counts. This can cause anemia which is characterized by a shortage of red blood cells, and thrombocytopenia which means a low level of platelets in the blood causing increased bleeding and bruising. Another condition that can occur is leukopenia (a shortage of normal white blood cells), which can cause problems fighting infections.

Then, the abnormal antibodies produced by the cancerous plasma cells will have the same amino acid sequence and therefore the same structure. Remember that an antibody is a protein, and proteins are a polymer of amino acids. This amino acid sequence is determined by the genes since each gene consists of a sequence of nitrogenous bases and every three bases read by the ribosomal apparatus (the one that synthesizes proteins), an amino acid is synthesized. Therefore, if we consider that cancer originates from a single cell that suffered a mutation in its genes and began to divide in an uncontrolled manner, the daughter cells (which are genetically identical to the mother cell) derived from this mutated cell will have the same genes (in this case, altered because they divide and grow in an uncontrolled and deregulated manner). This tells us that a single mutated B cell gave rise to this cancer.

Which of the following organelles can be found in plants AND animals?
A. Mitochondria
B. Chloroplasts
C. Vacuole
D. Cell wall

Answers

Answer:

Mitochondria can be found in plants and animals

Analyze the property of water you investigated and provide some real-world applications of the importance of this property of water. I did Surface Tension. Please Help!

Answers

Answer:

Water (H

2O) is a polar inorganic compound that is at room temperature a tasteless and odorless liquid, which is nearly colorless apart from an inherent hint of blue. It is by far the most studied chemical compound[18] and is described as the "universal solvent"[19] and the "solvent of life."[20] It is the most abundant substance on the surface of Earth[21] and the only common substance to exist as a solid, liquid, and gas on Earth's surface.[22] It is also the third most abundant molecule in the universe (behind molecular hydrogen and carbon monoxide).[21]

Which of the following best
describes what a constraint is
when you are designing
something to solve a problem?
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problem
B. A constraint is information that you can read to help you solve
the problem.
C. A constraint is a limitation that must be taken into account
when inventing your design.

Answers

Answer:

C. A constraint is a limitation that must be taken into account when inventing your design.

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True
False

Answers

Answer:

Biomagnification results is higher in amount of toxin substances so the answer is true

Explanation:

Which answer option below describes the best way to increase the accuracy of a
confidence interval calculation?

Answers

The answer is c but this has to be twenty characters long

The accuracy of a confidence interval calculation can be increased from  95% to 99%.

Thus, option C is correct.

What is confidence interval?

A confidence interval contain a population parameter in a specified proportion of the time, which can be computed using the given confidence level from a limitless level of individual sample.

The proportion of the range consists of the true value of the factor that will be identical to the confidence level.

In most of the confidence interval examples, the confidence level chosen is 95%. and it can be raise up to 99%.

It cannot estimate the plausible values of the unknown parameter of the population.

Two types of confidence interval observed such as accuracy which is defined based on whether the confidence interval contains the true population parameter or not.

Secondly, the precision defined in terms of the width of a confidence interval.

Hence, option C is correct.

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The layer of soil that contains the most organic material is called the
a. A
C C
b. B
d D

Answers

Answer:

That layer is called the a horizon, so the answer is A.

Explanation:

What is the answer with explaining

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Answer:

EeWw

Explanation:

This question involves two genes; one coding for earlobes and the other for hairline. According to the question, one parent is homzygous recessive for earlobe but homzygous dominant for hairline i.e. (eeWW) while the other parent is homzygous dominant for unattached earlobes but homzygous recessive for hairline i.e. (EEww).

The cross between the two parents is as follows: eeWW × EEww. The following gamete combination will be produced by each parent:

eeWW - eW, eW, eW, eW

EEww - Ew, Ew, Ew, Ew

Using these gametes in a punnet square, the following proportions of offsprings will likely be produced:

All EeWw offsprings

Compared to ocean crust near deep-sea trenches, crust near ocean ridges is ____.

Select one:

a.
younger


b.
the same age


c.
older


d.
magnetically reversed

Answers

Answer:

a. younger

Explanation:

HI friends good morning

The answer is C. Because it’s going to be older if it was created before the ocean crust .

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RBC, WBC, platelets
RBC, platelets, WBC
WBC, platelets, RBC
WBC, RBC, platelets

Answers

Answer:

WBC, RBC, platelets

Explanation:

Took a medical class

Which of the following is not typical of a capillary?

Group of answer choices

Virutally all fluids pushed out at a capillary bed are taken up again

The exchange of substances between the blood and interstitial fluid takes place across the thin endothelial walls of the capillaries

Osmosis from blood proteins tend to pull fluid back in

Blood pressure tends to drive fluid out of capillaries

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Answer:

its it the first options

Explanation:

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What did each of the bottles represent? What does the water represent?

Answers

The bottles represent the world, and the water represents the people inside the world

Answer:

The bottles represent the left and right sides of the heart. The water represents blood that flows through the circulatory system.

Explanation:

A student wants to determine how his classmates feel about school. He does a survey away from school so all participants will be willing to answer freely. In several cases, younger siblings of his classmates are present, so he lets them take the survey too. He surveys a total of 50 students. Has he done a well-designed, controlled experiment

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Answer:

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3. What is the term for movement of molecules from an area of relatively lower to higher concentration that requires energy?

passive transport

diffusion

active transport

osmosis

Answers

active transport (the answer)

What will be produced if their DNA molecule is replicated?

Answers

Answer:

the dna would be the same.

In transcription, the DNA sequence of a gene is transcribed (copied out) to make an RNA molecule. Transcription is the first step in gene expression. It involves copying a gene's DNA sequence to make an RNA molecule.

Explanation:

Si el sobrino de mi papá tiene un hijo que viene siendo mío?

Answers

Answer:

yo creon q sobrino lejan

o

Primo segundo creo que sería

Five more than the quotient of a number and eight is 42

Answers

Answer:

5+x/8=42

Explanation:

This is your answer to the equation.

If you want to solve it, then subtract 5 from both sides to get x/8=37. Then multiply both sides by 8 to get x=296

Hope this helps!! :)

Which is the main light-absorbing pigment for photosynthesis?
O carotene
O chlorophyll
O hemoglobin
O anthocyanin

Answers

Answer:

Chlorophyll

Explanation:

Chlorophyll, the primary pigment used in photosynthesis, reflects green light and absorbs red and blue light most strongly.

In plants, photosynthesis takes place in chloroplasts, which contain the chlorophyll.

Answer:

B) Chlorophyll

Explanation:

The chloroplasts, which contain the green pigment chlorophyll, are where photosynthesis takes place. Chlorophyll is the pigment that gives plants their distinctive color. It works by collecting the energy in the sunlight that strikes the plant. Because grass requires photosnthesis to create glucose, which is required for growth inside the grass, photosynthesis is critical to life on Earth. As a result, creatures that eat the grass get energy, and so on. As a result, photosynthesis is the mechanism through which energy is introduced into an environment.

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8.Explain how is small intestine designed to absorb digested food.
9. Write two differences between the nutrition in amoeba and human beings.
10. Fungus can be harmful and useful. Give an example showing both of these traits of fungus.

Answers

Answer:

10. Fungus are useful as they act as decomposers and decompose the dead things. Fungus are harmful as they can decompose fruits, grains and plants etc.

• Describe genetic______ and gene_________ as mechanisms of evolution.

Answers

Answer:

genetic is branch of science deal with study of heredity and genes

genes is segment of DNA which transfer parental character from one generation to another

Which of the following is not an example of where primary succession typically occurs?

After a fire
A retreating glacier
Emerging islands
Formation of new lakes

Answers

Answer:

After a fire

Explanation:

Primary succession is one of the two types of ecological succession in which a barren area of land (with no soil) is colonized by organisms called PIONEER SPECIES for the very first time.

Primary succession is characterized by a BARREN area that had no form of previous growth or colonization. These barren area can include a retreating glacier, an emerging islands or the formation of new lakes.

Note that, the succession that occurs AFTER A FIRE or any form of environmental disturbance is SECONDARY SUCCESSION.

A tall plant is crossed with a short plant, and the progeny are all intermediate in size between the two parental plants. If the intermediate F1 progeny were allowed to self-pollinate, and the F2 progeny were also intermediate in size, but following a normal distribution, this would suggest

Answers

Answer:

A tall plant is crossed with a short plant, and the progeny are all intermediate in size between the two parental plants. If the intermediate F1 progeny were allowed to self-pollinate, and the F2 progeny were also intermediate in size, but following a normal distribution, this would suggest polygenic inheritance.

Explanation:

Two or more genes contribute to the determination of a certain trait in polygenic inheritance.

What is polygenic inheritance?

When a tall plant and a short plant are crossed, all the offspring fall between the two parents plants in terms of height.

It would indicate polygenic inheritance if the intermediate F1 progeny were permitted to self-pollinate and the F2 progeny had intermediate sizes and followed a normal distribution.

Consequently, one personality trait is managed through is controlled by at least two other genes, at least two additional genes.

Therefore, If the intermediate F1 progeny were allowed to self-pollinate, and the F2 progeny were also intermediate in size, but following a normal distribution, indicate polygenic inheritance.

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All theories are hypotheses, but not all hypotheses are theories. Francis wants to know if a specific hypothesis he is researching is a theory. Which describes how Francis would know the hypothesis is a theory?
He could restate the hypothesis as a fact and no longer an explanation.
He could determine whether the hypothesis is based on repeated experiments.
He could perform the experiment and adjust data to match the desired results.
He could design and perform a new controlled experiment to test the hypothesis.

Answers

Answer:

He could determine whether the hypothesis is based on repeated experiments.

Explanation:

A theory is a hypothesis that has been proven correct by many expirements.

Francis could design and perform a new controlled experiment to test the hypothesis to determine whether it is a theory. Therefore, the correct statement is option D.

What is a theory?

A hypothesis is a scientific explanation for a phenomenon that is based on prior observation. However, a theory is a well-supported and widely accepted explanation for a natural phenomenon that has been tested and based on scientific fact.

A hypothesis has the potential to become a theory if tested through carefully designed and controlled experiments by collecting data needed for the experiment.

If the results of an experiment conducted by Francis are consistent with the hypothesis, the information can be used to develop a theory that can be used to explain a broader range of phenomena. However, if the results are not by the hypothesis, Francis cannot consider the hypothesis as a theory that must be supported by evidence.

Therefore, Francis needs to design and perform a new controlled experiment to test the hypothesis to determine whether it is a theory.

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The frequency of a lethal allele in a population is greatest when it is: Group of answer choices dominant manifested in infancy recessive co-dominant

Answers

Answer:

recessive

Explanation:

A lethal allele is a gene variant associated with a mutation in an essential gene, which has the potential to cause the death of an individual. In general, lethal genes are recessive because these alleles do not cause death in heterozygous individuals, which have one copy of the normal allele and one copy of the allele for the lethal disease/disorder. In recessive lethal diseases, heterozygous individuals are carriers of the recessive lethal allele and can eventually pass the 'defective' allele on to offspring even though they are unaffected; whereas dominant lethal diseases are caused by dominant lethal alleles, which only need to be present in one copy to be fatal. In consequence, the frequency of recessive lethal alleles is generally higher than dominant lethal alleles because they can be masked in carrier individuals. Some examples of human diseases caused by recessive lethal alleles include, among others, Tay-Sachs disease, sickle-cell anemia, and cystic fibrosis.

The frequency of a lethal allele in a population is greatest when it is: C. recessive.

A lethal allele can be defined as an allele that is responsible for the death of a living organism, especially by preventing its development. Thus, a lethal allele causes a complete mortality in the living organism carrying it, in a hom-ozygous condition.

Basically, a lethal allele is recessive in nature because it is expressed in the phenotype of an organism. Some examples of diseases caused by lethal alleles in humans are:

Cystic fibrosisSickle-cell anemiaAchondroplasia

In conclusion, a lethal allele that is recessive has the greatest frequency in a population.

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A solution that has a pH of 6.8
a. is acidic.
b. is basic.
C.
is alkaline.
d. has a neutral pH.
PLS HELPPPP

Answers

A solution that has a pH of 6.8

it is acidic as it has got ph value less than 7....But as it has got ph value near to 7 it is weak acidic..

calculate fcr on chicken ??

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Answer:

whaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaattttttttttt?

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