The oxidase test is another biochemical test used to distinguish aerobic versus anaerobic metabolism in microbes. Research the oxidase test and describe how it is performed. Include examples of oxidase-positive and oxidase-negative microbes in your answer.

Answers

Answer 1
The Bacillus genus is usually negative in regards for the Oxidase test.
Answer 2

Oxidase test is a biochemical test that is used to identify organisms that produce the enzyme  oxidase.

Oxidase test is used to distinguish aerobic from anaerobic metabolism in microbes.

Aerobic metabolism is the use of oxygen to generate energy from a food source while anaerobic metabolism is the generation of energy with the absence of oxygen.

The procedure of oxidase test includes:

Place a piece of filter paper in a clean petri dish and add 2 or 3 drops of freshly prepared oxidase reagent.Using a piece of stick or glass rod (not anoxidized wire loop), remove a colony of the test organism and smear it on the filter paper.Look for the development of a blue-purplecolour within a few seconds as shown in colour

Blue purple colour signifies oxidase positive organisms which includes Pseudomonas aeruginosa

Colourless signifies oxidase negative organisms which includes Escherichia coli

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Related Questions

WRITE THE DEFINITIONS OF EACH ANIMAL ORGANELLE LISTED. (1) nucleolus (2) nucleus (3) ribosome (4) vesicle (5) rough endoplasmic reticulum (6) Golgi apparatus (7) cytoskeleton (8) smooth endoplasmic reticulum (9) mitochondria (10) vacuole (11) cytosol (12) lysosome (13) centriole

Answers

Answer: nucleolus: a small dense spherical structure in the nucleus of a cell during interphase. nucleus: the positively charged central core of an atom, consisting of protons and neutrons and containing nearly all its mass. ribosome: a minute particle consisting of RNA and associated proteins found in large numbers in the cytoplasm of living cells. They bind messenger RNA and transfer RNA to synthesize polypeptides and proteins. vesicle: a small fluid-filled bladder, sac, cyst, or vacuole within the body. rough endoplasmic: reticulum (RER), series of connected flattened sacs, part of a continuous membrane organelle within the cytoplasm of eukaryotic cells, that plays a central role in the synthesis of proteins. Golgi apparatus: a complex of vesicles and folded membranes within the cytoplasm of most eukaryotic cells, involved in secretion and intracellular transport.

Explanation:

Which of the following is true
about true-breeding plants if they are allowed to self-pollinate?

A. They never have offspring with the same traits as the parent plant.

B. They have offspring with traits that are identical to the parent plant with one or two differences.

C. They always have offspring with traits identical to the parent plant.

Answers

Answer:

b

Explanation:

some are the same while others are different (traits)

What are altitudes? help pls

Answers

Answer:

Altitude is how high vertically something is by definition

1 : height above a certain level and especially above sea level. 2 : the perpendicular distance from the base of a geometric figure to the vertex or to the side parallel to the base.

What is the role of RNA?

Answers

Answer:

It is the 1st one ○ to provide the original blueprint for protein production

Explanation:

RNA polymerase (green) synthesizes RNA by following a strand of DNA. RNA polymerase is an enzyme that is responsible for copying a DNA sequence into an RNA sequence, duyring the process of transcription.

write paragraph about digestion of food in brief​

Answers

Answer:

Digestion is the breakdown of large insoluble food molecules into small water-soluble food molecules so that they can be absorbed into the watery blood plasma. In certain organisms, these smaller substances are absorbed through the small intestine into the blood stream.

Explanation:

according to wikipedia!

Digestion is nothing but breaking down of substances into simpler forms, which the body can use. We eat many things and they have very complex compounds, our body cannot use these compounds. So, different parts of our digestive system breaks down different compounds to simpler forms, so that our body can use it for different functions.

Without replication, organisms could not successfully


move.

grow and reproduce.

feed.

make proteins.

Answers

Answer: grow and reproduce

Explanation:

What is a plant nucleus

Answers

Answer:

All plant cells contain a nucleus, a structure that stores DNA and acts as a cell's command center. It is surrounded by the nuclear envelope and is filled with nucleoplasm. ... DNA is copied during DNA replication for new cells, as well as copied to RNA to make proteins in the nucleus.

Which food has the greatest amount of polyunsaturated fat?

Answers

there are multiple foods that do, some examples are:
walnuts
sunflower seeds
fish
There are multiple foods

what will most likely happen if a population is large and no migration, mutation, or environmental change occurs?
1. natural selection will increase
2. nonrandom mating will start to occur
3. the rate of evolution will increase
4. gene frequencies will remain constant

Answers

4. Gene frequencies will remain constant

The Earth and the moon are kept in orbit by gravity and mass. Please select the best answer from the choices provided T F

Answers

Answer:

True

Explanation:

the Earth and the Moon are kept in orbit by gravity and mass.

Answer:

True (T)

Explanation:

Yeah, the Earth and the moon are kept in orbit by gravity and mass. Hence, this statement is True (correct).

Phân tích các yếu tố cho thấy ĐẤT là môi trường đa dạng nhất về các loại sinh vật?

Answers

.... what you wrote isn’t a question.... you wrote a statement.....

Here is what you wrote translated to English

Analysis of factors shows that SOIL is the most diverse environment in terms of organisms?

Answer:

I don't no answer sorry

Explanation:

follow

When curare, a neuromuscular poison, is dropped onto an isolated muscle-nerve preparation in a laboratory, the muscle does not contract when the nerve is stimulated, even though neurotransmitter is released from the nerve cell. Why does this happen

Answers

Answer:

Explanation:

Whenever the nerve terminal's action potential depolarizes, calcium enters the cell through the channels. Calcium allows acetylcholine (ACh) to be released more easily.  These ACh molecules subsequently circulate into the synaptic cleft and attach to the motor end-plate of the nicotinic cholinergic receptors. Acetylcholine molecules connect to nicotine ion-channel receptors present on cell membrane the of muscle cells, enabling the ion channels to open. Calcium ions then enter the muscle cell, kicking off a series of events that eventually result in muscular contraction.

Curare is a kind of neuromuscular relaxant blocker that is non-depolarized in nature. Nondepolarizing muscle relaxants work as competitive agonists, binding to ACh receptors but failing to activate ion channels. As a result, they inhibit ACh from bonding, preventing the development of endplate potentials and inducing muscle paralysis.

a. Why was it important in this case to identify Salmonella Typhi in the feces of the restaurant worker?​

Answers

Answer:

Some other types of Salmonella cause typhoid fever or ... Most people with Salmonella infection have diarrhea, fever, and ..

Explanation:

Add as brainlist

Drag the tiles to the correct boxes to complete the pairs.
Match the mRNA sequences to their DNA sequences.
AUUACGCAU
CCGAAAUGU
GAUCAUUAC
UUUUUAACG
AAAAATTGC
arrowRight
GGCTTTACA
arrowRight
TAATGCGTA
arrowRight
CTAGTAATG
arrowRight

Answers

Answer:

mRNA sequence1=AUUACGCAU

DNA sequence1=TAATGCGTA

mRNA sequence2=CCGAAAUGU

DNA sequence2=GGCTTTACA

mRNA sequence 3=GAUCAUUAC

DNA sequence3=CTAGTAATG

mRNA sequence4=UUUUUAACG0

DNA sequence4=AAAAATTGC

14. What do the embryos of different vertebrate species have in common?​

Answers

Answer:

Similarities in embryos are evidence of common ancestry. All vertebrate embryos, for example, have gill slits and tails. Most vertebrates, except for fish, lose their gill slits by adulthood.

Explanation:

itz from gologolo lol I hope it helps uh

keep smiling #smilies

What is the complimentary dna strand for TCTTAAATGATCGAT

Answers

Answer:

AGAATTTACTAGCTA

Explanation:

The DNA strand pairs are:

Adenine (A) -- Thymine (T)

Cytosine (C) -- Guanine (G)

and vice versa.

The given strand:

TCTTAAATGATCGAT

Based on the given pairs above, your answer will be:

AGAATTTACTAGCTA

~

Z 00m 336"083"2553 (wZE2XQ)​

Answers

Bjsjhehsbdbdhhdvhdvxbdgvdbxhsbd

Which of the following findings would lead you to determine that an infant's airway is open

Answers

Answer:

blowing air into his/her mouth and checking for an increase in the thoracic region........I GUESS

The bacterium Methylophilus methylotrophus can use methanol and ammonia for protein synthesis. The glutamate dehydrogenase gene from E. coli was introduced into M. methylotrophus using recombinant DNA techniques. Under high ammonia conditions, the introduction of the glutamate dehydrogenase gene into M. methylotrophus resulted in an increase in protein yield. Select the statements that are reasonable explanations for the increased protein yield in the modified M. methylotrophus. Glutamate dehydrogenase catalyzes the formation of glutamate and glutamine in a two-step process. Both glutamate and glutamine play a key role in amino acid synthesis, thus an increase in levels of glutamate and glutamine increase protein synthesis. Glutamate dehydrogenase has a low affinity for NH^+_4, and can only catalyze the formation of glutamate from alpha-ketoglutarate and NH^+_4, when the concentration of NH^+_4 is high. Glutamate dehydrogenase has a high affinity for NH^+_4, thus the rate of glutamate synthesis increases exponentially under high ammonia conditions. Glutamate is the amino group donor for most transamination reactions involving amino acids, thus an increase in glutamate concentration increases protein synthesis.

Answers

Answer:

- Glutamate dehydrogenase has a low affinity for NH+4, and can only catalyze the formation of glutamate from alpha-ketoglutarate and NH+4, when the concentration of NH+4 is high.  

- Glutamate is the amino group donor for most transamination reactions involving amino acids, thus an increase in glutamate concentration increases protein synthesis.

Explanation:

Glutamate dehydrogenase (GLDH) is an enzyme found in prokaryotic and eukaryotic cells, which is known to catalyze the reversible oxidative deamination of glutamate (Glu) to alpha-ketoglutarate and ammonia at the same time the oxidation of NADH to NAD. In bacteria, this enzyme (GLDH) is efficient only at high concentrations of the ammonium cation (NH4+), because it has a low affinity for NH4+. Moreover, transamination is a pathway that involves the transfer of an amino group from one amino acid to a ketoacid in order to form new amino acids (this reaction is responsible for the deamination of most amino acids). Glu is the donor of amino groups in most of the reactions catalyzed by enzymes involved in the transamination between an amino acid and an alpha-keto acid. In consequence, in presence of GLDH, a high concentration of Glu will increase the production of new amino acids and therefore also protein synthesis.

How many kinds of mosquito are common in Nepal l​

Answers

there are 130 kinds of mosquito are common in Nepal l

•. otal genomic structure of an organism inherited from parents is known as -
a) Gene
b) Allele
c) Phenotype
d) Genotype​

Answers

I think the answer is a sorry if I get it wrong

Explain why you would recommend the nguni cattle meat to consumers in your locality.​

Answers

Answer:

Below

Explanation:

All different combinations were catalogued in the beginning of the century by a South African herdmaster. This work inspired the Nguni Cattle Register, a compilation of terms to describe in full a Nguni cow or bull. The cattle are medium-sized, with bulls weighing between 500 and 600 kg,[3] while cows weigh between 300 and 400 kg.

definition of human rights

Answers

Answer: a right that is believed to belong justifiably to every person.

Explanation:

Answer:

Human rights are standards that allow all people to live with dignity, freedom, equality, justice, and peace. Every person has these rights simply because they are human beings.

Explanation:

Human rights are rights inherent to all human beings, regardless of race, sex, nationality, ethnicity, language, religion, or any other status. Human rights include the right to life and liberty, freedom from slavery and torture, freedom of opinion and expression, the right to work and education, and many more.

Prepare a collage of tools and equipment used in production of any one agricultural crop, from seed or planting through packaging of the produce

Answers

Answer:

i think

Explanation:

Which of the following techniques employs a probe consisting of a labeled, single-stranded DNA of known sequence that can find and bind to a complementary sequence present in a large sample of different DNA sequences?

a. DNA hybridization
b. RFLP
c. PCR
d. DNA sequencing

Answers

Answer:

Explanation:

PCR

a phlebotomy technician has collected a neonatal screening card. which of the following actions should the technician take to properly dry the blood stop collection card?

Answers

Answer:

place the card on a flat dry surface

Scientists have been studying the size of three populations in a river in Ireland. In this ecosystem, pike (a type of big fish), and perch eat snails. The data showed that all three populations were stable. Then the snail population increased suddenly. What will likely happen to the size of the perch population as a result? The size of the perch population will...

Options:

A. Stay the same. The size of it's consumer population did not change, so the number of deaths in the perch population did not change. There will be the same number of births and deaths in the perch population.

B. Decrease. The larger snail population will take more energy storage molecules from the ecosystem, leaving fewer available for the perch population. This will lead to more deaths than births in the perch population.

C. Increase. The larger snail population will provide more energy storage molecules for the perch population, so they will reproduce more. This will lead to more births than deaths in the perch population.

D. Increase. An increase in any population within an ecosystem will lead to an increase in the sizes of all other populations in the ecosystem.

Answers

Answer:

C. Increase. The larger snail population will provide more energy storage molecules for the perch population, so they will reproduce more. This will lead to more births than deaths in the perch population.

Explanation:

A cell has twice as many mitochondria as a typical cell. It also has a large amount of rough ER. What functions
might that cell have?

Answers

Answer:

below

Explanation:

Select the correct answer.
How does the high specific heat of water help maintain the temperature of earth?
A By heating and cooling more slowly than land or air
B. By heating and cooling faster than land or air
с.
By heating faster but cooling more slowly than land or air

Answers

i’m pretty sure it is B

The high specific heat of water help maintain the temperature of the Earth by heating and cooling more slowly than land or air. Thus, the correct option is A.

What is the specific heat of water?

The specific heat of an object is the amount of heat which is required to increase or raise the temperature of unit mass of that particular substance through one degree celsius. Specific heat is represented by the symbol 'c'. Its unit in SI system is Joule /(kg °C) and in CGS is cal / (gm °C).

The high specific heat of water helps in regulating the rate at which air changes its temperature, which is why the temperature of the planet change between different seasons is gradual rather than sudden, especially the geographical locations near the ocean.

Therefore, the correct option is A.

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Darwin’s finches evolved on an island. What is the main reason that islands often provide good examples of evolution?

Answers

Islands are isolated

Answer:

They allow for automatic biogeographic isolation. In rock layers, jellyfish fossils are found lower than trilobite fossils, and trilobite fossils are found lower than ammonite fossils.

Explanation:

(found it on quizlet feel free to rewrite in you own words)

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