What is the application of Gene Editing in Primary T Cells?

Creative Biogene CRISPR/Cas9 Platform provides comprehensive gene editing services for various cell lines, especially for primary T cells. With years of experience, our talented scientists have established an efficient assay to edit genome in T cells by CRISPR/Cas9. At CRISPR/Cas9 PlatformCB, we offer a gene knockout service for customer as well as isolation of primary T cell service. Based on our excellent platform, our staff are focusing on supplying the best services and products.

https://www.creative-biogene.com/crispr-cas9/service/gene-editing-in-primary-t-cells.html

Answers

Answer 1

Answer:

Creative Biogene CRISPR/Cas9 Platform provides comprehensive gene editing services for various cell lines, especially for primary T cells. With years of experience, our talented scientists have established an efficient assay to edit genome in T cells by CRISPR/Cas9. At CRISPR/Cas9 PlatformCB, we offer a gene knockout service for customer as well as isolation of primary T cell service. Based on our excellent platform, our staff are focusing on supplying the best services and products.


Related Questions

If the complex cholesterol- HRP would not be commercially available, use the knowledge you learnt and internet to determine an alternative way for detecting cholesterol.

Answers

Answer:

Classical chemical methods, enzymatic assays, gas chromatography (GC), liquid chromatography (LC), and mass spectrometry (MS)

Explanation:

Classical chemical methods, enzymatic assays, gas chromatography (GC), liquid chromatography (LC), and mass spectrometry (MS) are the alternate methods used for the detection of cholesterol. These methods are used when HRP would not be commercially available for the analysis or detection of cholesterol in the patient's body so these methods can be used for the purpose of detection of cholesterol.

( PLEASE ANSWER IT FAST) Label the Organelles of a Cell
Identify the organelles in the cell to the right.

Answers

i think i'm too late to help D:

Answer:

A-vacuole

b- chloroplast

c-cell membrane

d- golgi

e- endoplasmic

f-cell wall

Explanation:

The switch from mitosis to meiosis in spermatogenesis is regulated by _____ levels of _______ and this is induced by _________. Fill in the blanks. Group of answer choices low, glial cell derived neurotrophic factor (GDNF), luteinizing hormone (LH) high, glial cell derived neurotrophic factor (GDNF), luteinizing hormone (LH) low, testosterone, follicle stimulating hormone (FSH) low, glial cell derived neurotrophic factor (GDNF), follicle stimulating hormone (FSH)

Answers

Answer:

1 - Follicle stimulating hormone FSH

2 - Luteinizing hormone LH

3  - Glial cell derived neurotrophic factor

Explanation:

Follicle stimulating hormone is a pituitary gland located underneath brain. It plays an important role in sexual development. FSH level in women stimulates egg production in ovaries and regulates menstrual cycle. In men FSH helps to control sperm production.

The switch from mitosis to meiosis in spermatogenesis is regulated by _____ levels of _______ and this is induced by _________.

Follicle-stimulating hormone Luteinizing hormoneGlial cell-derived neurotrophic factor

What are the roles of mitosis and meiosis in sperm development?

Somatic cells experience mitosis to increase, the germ cells experience meiosis to produce haploid gametes (the sperm and the egg).

The evolution of a new offspring organism is then started by the fusion of these gametes at fertilization.

Thus, Follicle-stimulating hormone, Luteinizing hormone, Glial cell-derived neurotrophic factor.

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What is socialization​

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As this question as biology at the top,socialization is a social process with strong biological components that interact with social and cultural frameworks and factors

9.5 What are the similarities and differences between reproduction in amoeba and reproduction in hydra?​

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Answer:

similarities: 1 they both reproduce asexually

Differences: 1 hydra reproduce through a process called budding whereas                              amoeba reproduce through a process called binary fission.

                    2 budding in hydra-  results in symetric division.

                       binary fission in amoeba- results in unsymetrical division.

Explanation:

Amoeba reproduces by a process known as binary fission in which parent cell divides into two equal daughter cells. Whereas in hydra reproduction occurs by budding. In budding, the bud is formed from the parent individual whereas in binary fission no bud formation occurs

If two haploid sperm fertilize a single haploid ovum, the resulting zygote would be considered a ________.

Answers

the answer:
triploid

What process in biology requires energy from a cell?

Answers

Answer:

I would assume you are talking about just basic general biology, in which case it's probably just looking for cellular respiration.

Explanation:

Metabolism is a combination of chemical reactions that are spontaneous and release energy and chemical reactions that are non-spontaneous and require energy in order to proceed. Living organisms must take in energy via food, nutrients, or sunlight in order to carry out cellular processes.

Besides oxygen exchange, the respiratory system also functions to eliminate (Click to select) . Too much of this gas can cause a(n) (Click to select) in blood pH, compromising homeostasis.

Answers

Answer: Carbon dioxide, decrease

Explanation:

Cellular respiration is a set of reactions by which organic compounds (sugars) are degraded by oxidation to inorganic substances. This process provides energy in the form of ATP and to achieve this, many reactions occur in which electrons are passed from one molecule to another, called redox reactions. In the case of aerobic respiration, the final electron acceptor is molecular oxygen, which is reduced to water.  Oxygen, like any gas, crosses the plasma membrane and then the mitochondrial membrane, and in the mitochondrial matrix it binds electrons and protons, forming water. It is in this final oxidation and in previous processes that energy is obtained. The reactions that extract energy from sugars such as glucose are catabolic reactions since a large molecule is broken into smaller molecules. For example, when glucose is broken down in the presence of oxygen, six molecules of carbon dioxide (CO2) and six molecules of water are obtained. Both CO2 and water are eliminated by lung exhalation.

In the event that this carbon dioxide is not completely eliminated, something called respiratory acidosis occurs, which is one of the disorders of the acid-base balance due to a decrease in the frequency of respirations or hypoventilation. The increase in the concentration of carbon dioxide in the blood causes the pH value to decrease. This scale is a measure of the degree of acidity or alkalinity of a substance or solution. A pH value of less than 7 means it is more acidic, and a pH value of more than 7 means it is more alkaline. Carbon dioxide is constantly produced as the body metabolizes energy, which will accumulate in the circulation rapidly if the lungs do not remove it properly. Alveolar hypoventilation thus leads to an increase in arterial CO2 partial pressure. The increase in pressure in turn decreases the ratio of bicarbonate (HCO3) to bicarbonate pressure with a consequent increase in acidity or decrease in blood pH. The body's metabolism rapidly generates a large amount of carbonic acid (H2CO3) and non-volatile acid. The lungs normally excrete the volatile fraction through ventilation without accumulation of acid in the blood but in this case this is not possible and this accumulation of carbonic acid causes the pH to rise. This disrupts homeostasis, a form of equilibrium that is achieved through a network of feedback control systems that constitute the self-regulatory mechanisms.

If segmentation has been done correctly, then the segments should reflect homogeneity within each segment and across/ between segments. This statement is ______________

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Answer:

The correct answer is - true.

Explanation:

The given statement is true as common marketable traits are observed between the segments it would represent homogeneity. This same segment would be representing homogeneity within each segment and between segments that would be heterogeneity in order to propagate the market.

If segmentation has been done correctly, then the segments should reflect homogeneity within each segment and across/ between segments. This statement is TRUE.

In biology, segmentation refers to the embryonal process where different parts of the body are formed during development.

Segmentation can be defined as the repetition of two or more body units along the anterior-posterior axis.

Segmentation is a fundamental process in insect and mammal development.

In conclusion, if segmentation has been done correctly, then the segments should reflect homogeneity within each segment and across/ between segments. This statement is TRUE.

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Pleasssee helepppppp

Answers

THE NASAL CAVITY IS A HOLLOW SPACE WITHIN THE NOSE AND SKULL THAT IS LINED WITH HAIRS AND MUCUS. THE FUNCTION INTO WARM, MOISTURIZE, AND FILTER AIR ENTERING THE BODY BEFORE IT REACHES THE LUNGS

Which is not true regarding the mammalian cardiac cycle

Group of answer choices

both atria contract at roughly the same time, and then the ventricles

relaxation, or filling, phase is called diastole

the semilunar valve is the usual pacemaker

contraction, or pumping, phase is called systole

Answers

the semilunar valve is usual peacemaker

What is the role of an indicator? Predict how the glucose test strips and iodine would react with the following foods:

Grape
Tomato
Summer squash
Orange
Bell pepper

Answers

Answer:

Tomato

Explanation:

The density of protists living in the hay infusion is ______________ than the regular pond water.

a. significantly lesser
b. lesser
c. about the same
d. greater
e. significantly greater

Answers

Answer:

a. significantly lesser

Explanation:

The density of protists living in the hay infusion is significantly lesser than the regular pond water because protists likes to live in aquatic or any water bodies. Some protists float on the surface of the water making their own food like plants by using the process of photosynthesis. Aquatic environments are ideal environment for protists, which have cilia and flagella that allows them to move in the water in search of food..

When the belt is moving at a high speed, 350 pills can be coated per hour. How many minutes would it take to coat all the pills in this batch at a high speed?

Answers

Answer:

6 pills are coated in one minute time.

Explanation:

There are 350 pills can be coated in 60 minutes due to high speed of belt movement which means that 6 pills are coated in one minute time period so if there are 60 pills so the belt takes 10 minutes to complete the coating of the pills. If there are 200 pills to be coated then we used the equation i.e.

6 pills -------- 1 minute

200 -------- x

By cross multiplication we get,

6x = 200 x 1

x = 200 / 6

x = 33.3 minutes

This means that in 33 minutes the belt can coated 200 pills.

Which is likely to be the first organisms to colonize an area after an disturbance like fire?
Lichens
Woody plants
Grasses
Shrubs

Answers

Answer:

Grasses

Explanation:

This question is describing secondary succession, which is the type of succession that occurs when a given area has being disturbed by natural disasters like hurricane, volcanic eruption, fire outbreak etc.

In secondary succession, the first set of organisms that recolonize the area after the disturbance are called PIONEER SPECIES. Pioneer species can include small annual plants like grasses. Hence, among the given options, GRASSES are likely to be the first organisms to colonize an area after a disturbance.

Explain the importance of nitrogen-fixing bacteria?​

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Nitrogen-fixing bacteria fix atmospheric nitrogen to ammonia, which can then be taken up by plants.

The process is called biological nitrogen fixation.

Rhizobium, Azotobacter, cyanobacteria are some examples of nitrogen-fixing bacteria, which may be free-living or symbiotic.

Match the organelle (1-4) with the correct description (5-8). 1) mitochondrion 5) synthesizing molecules 2) centriole 6) liquid in cell 3) endoplasmic reticulum 7) provides cell with energy 4) cytosol 8) aids the formation of the spindle apparatusA) 1 and 5, 2 and 6, 3 and 7, 4 and 8 B) 1 and 7, 2 and 6, 3 and 8, 4 and 5 C) 1 and 7, 2 and 8, 3 and 5, 4 and 6 D) 1 and 8,2 and 5, 3 and 6, 4 and7 E) 1 and 6, 2 and 8, 3 and 5, 4 and7

Answers

Answer:

C) 1 and 7, 2 and 8, 3 and 5, 4 and 6  

Explanation:

1 and 7: 1) mitochondrion; 7) provides the cell with energy

The mitochondrion is the organelle that produces the energy that the cell needs for its functions. It synthesizes adenosine triphosphate (ATP), which storages large quantities of energy in its bonds.

2 and 8: 2) centriole; 8) aids the formation of the spindle apparatus

The centrioles have a crucial role during mitosis. They form the spindle apparatus that attaches to the chromosomes, moving them to the opposite poles of the cell.

3 and 5: 3) endoplasmic reticulum; 5) synthesizing molecules

The endoplasmatic reticulum is a continuous membrane connected to the nuclear membrane. There are two types, the Rough Endoplasmatic Reticulum (RER) and the Smooth Endoplasmatic Reticulum (SER). The first one synthesizes proteins, while the second one synthesizes lipids.

4 and 6: 4) cytosol; 6) liquid in the cell

The cytosol is the liquid that is inside the cell. It is mostly water, but it also has ions and molecules. It is important for the transport of substances.

The correct match between organelles and their description is - Option C.  1 and 7, 2 and 8, 3 and 5, 4 and 6.

In the cell, there are many organelles that perform different and specific functions essential for the cell to survive. These organelles help in synthesizing molecules, energy production, cell division, and many others.

Mitochondrion: it is a cell organelle that is the site of cellular respiration, which is a process that breaks the sugar molecule and releases a high amount of energy in the form of ATP. Therefore, it is known as the powerhouse of the cell. Thus, correctly matches with 7) provides the cell with energyCentriole: It is organelles that help to form the spindle during the cell division in the cell that makes the region at the poles where spindle fibers form. Thus, it correctly matches with 8) aids the formation of the spindle apparatus.Endoplasmic reticulum: It is organelles that synthesize various molecules such as hormones and other protein molecules, Rough ER has ribosomes attached to it that synthesize proteins. Thus, correctly matches with 5) synthesizing moleculesCytosol: It is a semi-fluid-like substance made up of a lot of water, dissolved ions, smaller minute molecules, and proteins. It has filled in the interior cellular space. Thus, the correctly matches with 6) liquid in the cell.

Thus, the correct answer is - Option C.

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g Cerebrospinal fluid formed in the lateral ventricles travels through the ________ to reach the third ventricle.

Answers

Answer: Interventricular foramen

Explanation:

The cerebrospinal fluid is a fluid that covers the brain and spinal cord. It circulates through the subarachnoid space, the cerebral ventricles and the ependymal canal. Several diseases alter its composition and its study usually detects meningeal infections, carcinomatosis and hemorrhages. Some of its functions are:

Hydropneumatic support against local pressure for the encephalon.Eliminates metabolites from the central nervous system.Protects the central nervous system from trauma.

This fluid also fills the ventricles, which are large open structures deep within the brain and help keep the brain buoyant and cushioned. The lateral ventricles are the largest ventricles and connect to the third ventricle through the intraventricular foramen. This third ventricle is a narrow, medial cavity located between the diencephalic masses. Then, the interventricular foramina are channels that connect with the ventricles of the lateral walls and with the third ventricle, at the level of the midline of the brain. Like these channels, they allow cerebrospinal fluid to circulate through the rest of the ventricular system of the brain. The walls of the foramina also contain choroid plexuses, responsible for the production of cerebrospinal fluid, which continue in both the lateral ventricles and the third ventricle. After reaching the third ventricle, the cerebrospinal fluid travels through the median aperture into the subarachnoid space at the base of the brain.

Dalits are followed enters in temples true or false

Answers

Answer:

False A notice by the priest told dalits they can’t enter the temple because they are “not pure”

Which of the following is not a characteristic of pioneer species ?
They facilitate environmental change, making an environment more habitable for other species
They remain in an environment,even as more competitive species arrive
They tolerate a wide range of conditions
They are capable of colonizing a barren environment

Answers

Answer:

They remain in an environment,even as more competitive species arrive

Explanation:

Pioneer species, in ecological succession, are the first set of organisms to colonize a bare area of land or a disturbed area of land. Pioneer species include lichens, grasses, fungi etc.

Pioneer species have unique characteristics that distinguishes them from every other species of organism. These include:

- They facilitate environmental change, making an environment more habitable for other species.

- They tolerate a wide range of environmental conditions.

- They are capable of colonizing a barren environment, as in primary succession.

However, pioneer species do not remain in an environment, even as more competitive species arrive. They rather give way for new species to emerge and dominate.

A dihybrid cross is performed between a corn plant with the genotype YYSS and a corn plant with the genotype yyss. What percentage of offspring will be yyss

Answers

Answer:

0%

Explanation:

All corn plants will have the genotype YySs

The percentage of offspring that will be yyss is 0%.

In a dihybrid cross, the alleles for two different traits are considered. In this case, the traits are color (purple or yellow) and texture (smooth or wrinkled). The genotype of one parent is YYSS (homozygous dominant for both traits), and the genotype of the other parent is yyss (homozygous recessive for both traits).

According to the principles of Mendelian inheritance, when crossing two parents with different genotypes, the offspring will inherit one allele from each parent for each trait. In this case, the offspring will receive either a Y or y allele for color and an S or s allele for texture.

Since the parent with the genotype YYSS is homozygous dominant for both traits, all of its gametes will carry the Y and S alleles. On the other hand, the parent with the genotype yyss is homozygous recessive for both traits, so all of its gametes will carry the y and s alleles.

When these gametes combine during fertilization, all the resulting offspring will receive a Y allele for color and an S allele for texture, as there are no alternative alleles present in the parents. Therefore, none of the offspring will have the yyss genotype.

In summary, the dihybrid cross between the plants with genotypes YYSS and yyss will produce offspring with the genotype YySs, but none of the offspring will have the yyss genotype. Therefore, the percentage of offspring that will be yyss is 0%.

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The complete question is:

A type of corn has kernels that are either purple or yellow, and smooth or wrinkled. Yellow-Y- is dominant over purple-y- and smooth-S-is dominant over wrinkled --- A dihybrid cross is performed between a corn plant with the genotype YYSS and a corn plant with the genotype yyss. What percentage of offspring will be yyss? Select one or more:

a. 100% b. 80%c. 50% d. 25% e. 10%f. 0%

walking, running and carrying a book involve using ________ type of muscles.
a) cardiac
b)voluntary
c)involuntary
d)Striated​

Answers

Answer:

It is voluntary I believe.

Explanation:

Name two heart diseases in humans.​

Answers

Coronary artery disease and arrhythmia
Heart failures and strokes are two cardiovascular diseases (:

What are the 4 main parts to an amino acid

Answers

All amino acids have the same basic structure, which is shown in Figure 2.1. At the “center” of each amino acid is a carbon called the α carbon and attached to it are four groups - a hydrogen, an α- carboxyl group, an α-amine group, and an R-group, sometimes referred to as a side chain.Mar 6, 2021

Answer:

» A central alpha - hydrocarbon.

» A carboxyl functional group.

» And amine functional group.

» A side chain.

Would a bacterial cell undergo mitosis in the same way as a eukaryotic cell? Why or why not?

Answers

Unlike eukaryotes, prokaryotes (which include bacteria) undergo a type of cell division known as binary fission. In some respects, this process is similar to mitosis; it requires replication of the cell's chromosomes, segregation of the copied DNA, and splitting of the parent cell's cytoplasm.

​Which cellular component is common to all cell types?

Answers

All cells share four common components: 1) a plasma membrane, an outer covering that separates the cell's interior from its surrounding environment; 2) cytoplasm, consisting of a jelly-like region within the cell in which other cellular components are found; 3) DNA, the genetic material of the cell; and 4) ribosomes, .

Can bats eat butterflies?

Answers

Bat can eat butterflies, like most bugs. That being said they usually don’t eat butterflies as they are active at different times of the day then bats. Bat usually eat other bugs, such as moths, caterpillars, spider etc.

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Cuales son el grupo de briofitos más abundante

Answers

Answer:

Existe una clasificación para las plantas briofitas entre las que se cuentan: 1. Las Hepática, que se considera el mas diverso y como consecuencia hasta el mas abundante en el planeta ya que cuenta con al menos 8000 especies.

Explanation:

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1.Explain the energetic constraints that prohibit prokaryotes from getting larger than they are. Be sure to explain the implications of the energy canyon for the evolution of eukaryotes.

Answers

Answer:

Space constraints.

Explanation:

Space constraints are the energetic constraints that prevent prokaryotes from getting larger than their limit. Space constraints control the sizes of both the largest and smallest bacteria. Bacteria can't shrink more than they have already because there is not enough space left for DNA and necessary proteins. In the same way, the prokaryotes can't increase in size beyond their limit due to space constraints.

what are the steps of earthworm farming? Be concise in your points​

Answers

Answer:

Explanation:

8 Easy Steps to Your Own Worm Farm

1. Find Containers. You need a container that can drain to keep your worm farm in.  

2. Site Your Farm. Decide where you will put your worm farm on your plot.  

3. Add Bedding. The worms in your farm need material in which to live.  

4. Add Worms.  

5. Add Food.  

6. Cover.  

7. Maintain.  

8. Migrate Worms.

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help with 4b thank you. The correlation coefficient, r, between the prices of smartphones, x, and the number of sales of phones, y, equals 0.63.Select the statement which best describes the relationship between the price and sales.The value of r indicates that the number of sales decreases as the price decreases.The value of r indicates that the number of sales decreases as the price stays the same.The value of r indicates that the number of sales decreases as the price increases.The value of r indicates that the number of sales is not related to the price.I think its (C): The value of r indicates that the number of sales decreases as the price increases. The reactivity of elements can be predicted based on the position of an element in the periodic table. Based on the periodic table, which of the following elements would be expected to be the most reactive? Iodine (I)Iodine (I)Chlorine (Cl)Chlorine (Cl)Fluorine (F)Fluorine (F)Bromine (Br) Which artist photographed the above images? 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Then, answer the question.Voluntourism: An Opportunity Too Good to be TrueA Speech to the Student Body of Evergreen High[1] Picture this: It's Spring Break, and you fly off to some country where there's lush rainforests and beautiful, blue coastlines to explore. There's also people in need, so you decide to blend your vacation with volunteering. Volunteering as a tourist, or voluntourism, seems like a great way to explore new regions and help people at the same time. However, this "volunteer plus travel" experience can actually harm local communities. While many teens might view traveling and volunteering abroad as a worthwhile adventure, there are more genuine and effective ways to make a difference.[2] Most would agree that volunteering in general is a worthy use of time. However, what if you found out the children you are "helping" are actually being kept in poor conditions so voluntourists will spend money to come to the local area? Dale Rolfe, a supporter of ethical voluntourism, explains the shocking reality that "Animal sanctuaries and orphanages are often manufactured for the voluntourist...encouraging a cycle of exploiting the very animals and children the volunteers are trying to help."[3] Proponents of the "volunteer plus travel" experience also argue that traveling to new places builds character and is a valuable way to learn about different cultures. With voluntourism, however, participants often pursue experiences that are all about them. For example, they sign up to build a school for a gold star on their resume, but they have no real building skills and take jobs away from local construction workers (Schulten). Or, they arrive to teach English but instead take selfies with the locals. One world traveler and ethical voluntourist believes voluntourism "can perpetuate small minded views of the world by taking insulated, fake, and structured experiences and selling them as unabridged and eye opening" (Carlos). The voluntour experience is a mirage. The voluntourist's eyes are not opened to real life at the destination, and lasting change is not achieved.[4] If you want a genuine experience where you can see a lasting impact, there are better options than voluntourism. You can volunteer in your local community. Give an hour every week to your town's animal rescue. Serve monthly dinners to the homeless. Be a reliable, positive influence on a child who needs a mentor. Studies show that volunteering and forming lasting relationships with those you help has a positive impact on your physical and emotional health. In fact, blood pressure is reduced, memory is improved, and rates of depression are reduced (Michaels).[5] There is another reason to look into alternatives to voluntourism. Did you know the average "voluntour" travel package costs $3,400 (Rolfe)? Could that travel money be better spent? If the world's citizens are your passion, it could go to an international organization. If you care about education, your funds can be used to buy books for students in faraway lands. If you want villagers to have clean water, contribute funds to local efforts to dig wells. If you want to experience a different culture, travel to the country as a guest, and learn from the locals how you can best help them after you've returned home. But do not voluntour.[6] In reality, there are better ways to make a difference. Voluntourism might appear to be an adventure that blends travel and helping others, but it does little except provide a costly, superficial experience that might actually do more harm than good. So, volunteer where you are most needed-at home, where you can stay to see the job through and form genuine, lasting relationships. Choose a beautiful coastline closer to home and send the travel money you saved to an international organization that will put it to good use. Whatever you do, don't turn someone else's hardship into your vacation."The Opportunity of a Lifetime"A magazine advertisement with the title Both texts (the speech and the advertisement) address voluntourism. However, each text has a different purpose, which is reflected by the details each writer chose to include. Consider the words and images used.In a paragraph of 6-8 sentences, identify what each text emphasizes and explain how that emphasis reveals each author's position on voluntourism. Be sure to state each author's position and include evidence from both texts to support your analysis. En el escudo representa dos ramas una de laurel y otra de palma Por qu la rama de laurel tiene dos hojas cadas? * 10-1: Compound ProbabilityA desk drawer contains 5 red pens, 6 blue pens, 3 black pens, 24 silver paper clips, and 16 white paper clips. If you select a pen anda paper clip at random, what is the probability that you select a black pen and a white paper clip? Round to the nearest tenth of apercentO A. 11.72%B. 12.12%C. 9.9%O D. 8.6%