What is the difference between traditional PCR and qPCR?
a. Traditional PCR has only 1 cycle whereas qPCR has multiple cycles
b. In traditional PCR, the presence or absences of a DNA sequence is analyzed upon reaction completion whereas qPCR monitors DNA amplification as the reaction progresses
c. Traditional PCR is an outdated technique which is unreliable and has not been utilized for years whereas qPCR is a newer and better technique which is always preferred nowadays.
d. Traditional PCR is monitored by an increases in fluorescence signal whereas it is impossible to monitor qPCR by increases in fluorescence signal

Answers

Answer 1

Answer:

The difference between traditional PCR and qPCR (Real-time PCR) is:

b. In traditional PCR, the presence or absences of a DNA sequence is analyzed upon reaction completion whereas qPCR monitors DNA amplification as the reaction progresses

Explanation:

Real-time PCR (qPCR) is an advanced tool for analyzing RNA and DNA.  With Traditional PCR detection occurs at the end-point of the reaction, while with Real-time PCR, detection of the RNA and DNA sequencing is detected as the reaction is occurring.  The advantages of the qPCR over the traditional PCR include higher precision and resolution,  and increased dynamic range and sensitivity.

Polymerase Chain Reaction (PCR) is a widely-used Biology tool to rapidly replicate millions of copies of a specific DNA sample.


Related Questions

What came first? The chicken, or the egg?



:3​

Answers

Answer:

easy

Explanation:

the egg obviously

Michael discovers that a fish breathes less often in cool water. He correctly hypothesizes that A. the fish slows down in cool water, and does not need as much oxygen. B. the fish is eating, and does not have time to breathe as much. C. the fish is sleeping, and does not need as much oxygen. D. there is less oxygen in cool water, so the fish breathes slowly.

Answers

Answer: then it’s d there is less oxygen in cool water

Explanation:

There are many explanations for Michael's observation that fish breathe less frequently in cold water. However, his hypothesis that the fish returns to the cold water and requires less oxygen seems possible.

What type of animals fish are?

Fish are cold-blooded animals, which means their environment controls their body temperature. Their metabolic rate, activity level, and oxygen requirements decrease as water temperatures drop.

As a result, fish can slow down in cold water, reducing their oxygen demand and reducing their breathing rate.  Oxygen solubility may also play a role in whether fish breathe less in cold water. As the water temperature decreases, the ability of oxygen to dissolve in the water also decreases. Because of this, cold water can have less oxygen available, which can cause fish to breathe more slowly to compensate for the lack of oxygen.

Because fish need oxygen to digest food, they are likely to eat and breathe less. It's basically a joke that the fish are resting because fish don't have an obvious resting cycle and will move and swim when they are resting.

Finally, Michael's hypothesis that fish breathe less in cold water is plausible as it suggests that fish slow down and don't need as much oxygen.

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Why does warm air always rise at a front?

Answers

Warm air is less dense than the cool air that surrounds it so it rises above it

The adenine of the start codon is located at the +1 position. Use the DNA Mutations Interactive to determine which statements describe missense mutations. a substitution of G to U at position +13 in the sense strand a transition at position +12 in the antisense strand a transversion of C in the sense strand of the histidine codon a G nucleotide is inserted after position +1 in the antisense strand a deletion of the A at position +7 in the antisense strand an insertion of A between positions +7 and +8 on the sense s

Answers

Answer:

A transition at position +12 in the antisense strand

A deletion of the A at position +7 in the antisense strand

Explanation:

Given that Adenine of the start codon is located at the +1 position, The correct  statements that  describe missense mutations are

 A transition at position +12 in the antisense strandA deletion of the A at position +7 in the antisense strand

Adenine is a purine nucleobase that is used in the formation of nucleic acids

dsadasd das sad afsasdasdsa?

Answers

I am sorry what?????

what language is this?

Will give brainliest

NO LINK SHARING OR U WILL BE REPORTED

After an initial infection of the measles virus, B-cells recognize the measles virus in your body. How is this helpful in human immune response? (SC.912. L.14.52)
ws
O The B-Cells transfer this recognition to T-cells, which will then devour the viruses
o The B-cells use this recognition to defend the body against the B-cells more quickly recognize and respond to any other virus that invades the body
O The B-Cells more quickly recognize the response to any other virus that invades the body
O The B-cells produce antibodies more quickly if the measles virus is encountered again

Answers

Answer: it’s D

Explanation:

(I need help will make brainlyest) Which shows the correct order for the classification of species------------------

A) Domain, Kingdom, class, Order, Phylum, Family, Genus, and species-------------------------

B) Domain, Kingdom, Phylum, class, order, Family, Genus, and species--------------------------

C) Domain, Kingdom, Phylum, Class, Order, Family, Species, and Genus------------------------

D) Domain, Kingdom, Phylum, class, order, family, genus, species----------------------------------

Answers

i believe the answer is B thank you troy and abed

An ecosystem engineer can cause a cascade of effects including abiotic and biotic changes.
O True
False

Answers

Answer:

O True

Explanation:

Ecosystem engineers are able to modify the surrounding environment, either by creating new habitats or modifying/destroying existing ones to adjust them to their needs. These species can significantly alter their environments, having a large impact on the species richness as well as modifying the availability of abiotic factors (e.g., water, space, etc) of a particular area. In certain environments, ecosystem engineers can even act as keystone species. Some examples of ecosystem engineers include, among others, beavers, woodpeckers, corals, etc.

Infer how the structure of the root would be impacted in the absence of each of these
structures.

Answers

Answer:

I  cant see them

Explanation:

Please Provide a picture.

in selective breeding, the desirable traits are chosen.

a. true
b. false

Answers

True because they are selecting and breeding for traits that will be best for survival

CAN SOMEONE PLEASE HELP ME WITH THIS SCIENCE QUESTION

Answers

Answer:

D

Explanation:

Happy Days to you!

Answer D.) extremely high

When comparing the brightness of stars, it is
necessary to know the distance to each star.
vole
True
False

Answers

I believe it is true

12. The best plate to cultivate enteric bacteria is?
A. EMB
B. Blood agar
C. Mannitol salt
D. Sabouraud agar plate

13. The Ingredient in Hektoen plate which makes the agar selective is?
A. Salt
B. Glucose
C. Mannitol
D. Bile acids

14. The enteric plates are only allowing the growth of ?
A. Gram negative bacteria
B. Gram positive bacteria
C. Anaerobes

Answers

Answer:

A.) EMB (Eiosin Methylene Blue)

B.) Hektoen Agar contains Bile salt which makes it selective medium.

C.) Gram Negative

A prospective groom, who is healthy, has a sister with cystic fibrosis (CF), an autosomal recessive disease. Their parents are normal. The brother plans to marry a woman who has no history of CF in her family. What is the probability that they will produce a CF child? They are both Caucasian, and the overall frequency of CF in the Caucasian population is 1/2500—that is, 1 affected child per 2500. (Assume the population meets the Hardy-Weinberg assumptions.)

Answers

Answer:

Explanation:

From the given information:

Since the parent are normal; Let assume that their trait is Pp

The affected sister  will be recessive in nature, so the trait will be pp

Now, the husband who is the groom will be a carrier with a probability of 2/3 since her sister is affected.

Also, the wife will be carrying a heterozygous trait since she is normal as well.

However, using Hardy-Weinberg assumption:

[tex]q^2 = \dfrac{1}{2500}= 0.0004[/tex]

[tex]q = \sqrt{0.0004}[/tex]

q = 0.02

Recall that:

[tex]p+q =1 \\ p = 1 - q \\p = 1 - 0.02 \\p = 0.98\\[/tex]

Thus, [tex]p^2 = (0.98)^2[/tex]

[tex]p^2 = 0.9604[/tex]

[tex]2pq = 2 \times 0.98 \times 0.022[/tex]

2pq = 0.0392

Finally, the required probability that there will be an affected offspring amongst the family is:

[tex]= \dfrac{1}{4} \times \dfrac{2}{3} \times 0.0392[/tex]

[tex]= \dfrac{1}{153}[/tex]

List 3 inputs (things entering) for the leaf in Model 1.

Answers

Answer:

light energy,carbon dioxide, and water

Explanation:

Answer:

light energy,carbon dioxide , water

Search using the internet on how you can expand or enhance one's knowledge of animal/fish raising.​

Answers

Answer:

Fish farming involves raising a variety of aquatic animals, such as fish, mollusks and shrimps in an enclosure such as a tank or pond. With the surge in demand for healthy animal protein the  demand for fish and fish products is on the rise. Fish farms have the potential to produce fish for home consumption at about $0.90/lb which is a considerable saving when compared to supermarket prices of over $2.00/lb.

The first step for fish farming is to determine what you are going to do with your full-grown fish. You need to know the purpose, whether you are going to keep fish as a pet or for fish meat. This will decide a lot of things like the space requirements, equipment and capital requirements. If you intend to keep fish as a pet for showcase then a small tank or a backyard small fish farm is sufficient. If you intend to put a small fish farm with one or two ponds then also with a little business mindset you can market 1000 to 2000 lb of fish.  

This will also require you to set up a business entity and have a business mindset.The costs associated with raising different types of fish varies because different fish need certain temperature water to live. The type of fish also decides the requirement of equipment and the feed cost. There are lots of laws about raising fish and animals, and even more, stringent laws related to raising animals that are going to be consumed as food.

Before starting fish farms or fish hatchery of any size or type it is advisable to check local laws and the need of permits  to do so in your area.Normally you will get a license to operate your fish farm for a year which will be renewable. You need to submit the following documents to apply for the license:

Filled in application form

Sketch or aerial map of the facility layout, including dimensions

Copy of fish health certificate

Copy of stocking permit

Letter of a written permission from the landowner (in case if you are not the landowner)

Explanation

(I truly apologize if this answer is too long)

A. The capture/mark recapture method was used to give an accurate
estimate of the rhinoceros beetle population in the Western province. Traps
were set up and captured beetles were painted light brown as oppose to red an
yellow. All captured-recaptured were counted and recorded.
Total count
in
Total marked
Total counts
in
Total marked
40
40
15
35
Use the Lincoln Index to calculate the total beetle population?​

Answers

Do not click in the link above above , he is a scammer and trying to fool us. Report any accounts you see saying that and using that link.

PLS HELP ASAP!!!!
in complete sentences compare in depth neutrophils, eosinophils and the basophils.
Compare their functions
Compare their structures
Compare how they look when they are stained
Compare their counts

Answers

Answer: Neutrophils have a two-lobed or bilobed nucleus, while eosinophils have bean-shaped nucleus and basophils have a multi-lobed nucleus. The lifespan of neutrophils is 5-90 hours, whereas eosinophils lifespan is 8-12 hours and that of basophils have a life span of 60-70 hours. functions- All the granulocytes present in white blood cells play their role in fighting against pathogens and in destroying them, where neutrophils vital role is in engulfing the foreign or toxic particles through phagocytosis; Eosinophils are the first one in triggering the inflammatory response during allergic disorders; and Basophils prevents the immediate blood clotting, as it contains heparin (anticoagulant) in the body. structures- Neutrophils have a two-lobed or bilobed nucleus, while eosinophils have bean-shaped nucleus and basophils have a multi-lobed nucleus. ... Neutrophils make up to 40-75% of the total white blood cells, while eosinophils mark their presence up to 1-6% and basophils are up to 0.5-1% of the whole white blood cells.  stained- Neutrophils:Neutrophils are stained in natural pink color. Eosinophils: Eosinophils are stained in brick red in acidic stains. Basophils: Basophils are stained in dark blue in basic stains. counts- Neutrophils are one of the primary types of white blood cells, that evokes the immune response against any foreign particle attack in the body. Eosinophils are another type of white blood cells, that helps in fighting against disease and allergies. Basophils are such white blood cells that help to diagnose the specific health problems like autoimmune disease or other blood disorder, etc. in the body.

Explanation:

How does stimulus become a memory? stay safe god bless.

Answers

Memories will occur when specific groups of neurons have been reactivated. The stimulus results in any brain in a particular pattern of neuronal activity. Some neurons start to become active in either more or less a particular sequence. Retrieval Cue; a stimulus for remembering information.

The moon's relative motion causes
A)
ocean tides.
B)
wind currents.
the phases of the Moon.
D)
the rotation of the Moon.

Answers

Answer: the phases of the moon

Explanation: i did it

How are living organisms linked within an ecosystem? A. by their physical adaptations B. by what they eat C. by their size D. by their habitat
ILL GIVE BRAINLIST!!!PLEASE HELP!!​

Answers

Answer:

B

Explanation:

Living Organisms in an ecosystem have a food chain or energy chain. This chain links all organisms within an ecosystem, therefore, answer B "By what they eat" should be the correct answer

Organisms are linked within an ecosystem by their physical adaptations.

What is physical adaptation?

Physical adaptations are special body parts that help a plant or animal survive in an environment.

A physical adaptation is some type of structural modification made to a part of the body.

A behavioral adaptation is something an animal does - how it acts - usually in response to some type of external stimulus.

what is  an Ecosystem?

An ecosystem is made up of all species and the physical context in which they interact.

Nutrient cycles and energy flows connect these biotic and abiotic components.

Photosynthesis enters the system, absorbing energy and storing it in plant tissue.

Animals assist in the transmission of nutrients and energy throughout the system by eating plants and each other.

Both external and internal factors have an impact on ecosystems.

The availability of diverse resources within the ecosystem is determined by internal factors, whilst resource inputs are usually governed by external activities.

Ecosystem mechanisms thus regulate as well as control interior elements.

so the correct answer is option A.

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(By CMI W Sunce Source
1. What organism is pictured?
2. Name and describe the process is shown on the right.
3. What is the name of the circular bodies? What happens there?

Answers

Answer:

Explanation:

PLEASE HELP WITH THIS!!!

Answers

Answer:

my mama is my mom

Explanation:

mum gae

A substance whose shape can easily change is a
a.
solid.
b.
powder.
c.
fluid.
d.
metal.


Please select the best answer from the choices provided

A
B
C
D

Answers

Answer:

fluid.

Explanation:

because there molecules can freely move and take the shape of any container

I will do Brainliest If this is quick and right. (I will figure out brainliest ive never done it before)

Answers

epithelial stomach cell>stomach lining tissue>stomach>disgestive system

Ashton is a crime scene investigator. He ensures that all the evidence gathered at the scene is properly handled and labeled. What is Ashton protecting?
Ashton ensures that the (blank) the documentation of who possesses evidence at all times, is protected by properly handling and labeling evidence.

Answers

when it says (blank) it means evidence evidence.

How much has the greenhouse gas carbon dioxide increased in the air just in the last few years? ITS DUE IN A FEW HOURS PLEASE HELP!

Answers

Answer:

approximately 2.3 parts per million (ppm) per year

Explanation:

In 2019, the global amount of atmospheric carbon dioxide (CO2) has been estimated at approximately 410 parts per million (ppm). These levels are higher than at any time in the last 800,000 years, and it has been estimated that in 2025 the CO2 levels will be higher than at any time in the last 3.3 million years. In 2018, there has been an increase of approx. 2.5 ppm, the same increase observed between 2017 and 2018. Moreover, the average increase of the CO2 levels in the atmosphere between 2009 and 2018 was 2.3 ppm each year.

the cytoplasmic extensions that, together with the cell body, provide the main receptive surfaces for neurons are the:

a: axons
b: soma
c: dendrites
d: synapses

Answers

I’m pretty sure it’s dendrites
:)

You are tasked with characterizing responses to Epidermal Growth Factor (EGF) treatment of HeLa Cells. You grow HeLa cells then replace the culture media with media containing EGF at various concentrations, then harvest samples for Western blot analysis using an anti-phospho-MAPK antibody as a readout of receptor tyrosine kinase activity. As expected, your control (EGF treated cells) show a strong phopho-MAPK signal on your Western blot. What do you hypothesize will happen if you pre-treat HeLa cells with siRNAs (RNAi) against Ras prior to adding EGF and performing Western blot analysis with anti-phospho-MAPK

Answers

Answer:

The answer is "The EGF receptor will be activated but the signal will not be appropriate transduced"

Explanation:

It induces the replication of cell types, especially fibroblasts or epithelial cells. It enhances neuron variety's telling the difference, ripening, and survival. The proinflammatory cytokines of the EGF family are described by four disulfide bonds that confer adhesive properties. The EGF sensor is switched on and the signal is not transmitted correctly.

PLEASE HELP AND EXPLAIN (NO LINKS OR NO RANDOM ANSWERS FOR POINTS)

Answers

The dust may have caused plants to die which led to herbivores dying of starvation then the carnivores slowly started to run out of other dinosaurs to eat and they became extinct. (I hope this made sense and helped some!)
Other Questions
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