Answers
Answer:
The underlying principle of SEC is that particles of different sizes elute (filter) through a stationary phase at different rates. This results in the separation of a solution of particles based on size.
hope it helped
Size exclusion chromatography (SEC) separates molecules based on their size by filtration through a gel.
What is size exclusion chromatography?
Size exclusion chromatography (SEC) splits molecules based on their size by filtration via a gel. The gel consists of spherical beads including pores of specific size distribution. Separation happens when molecules of various sizes exist included or excluded from the pores within the matrix.
Size exclusion chromatography exists named gel filtration chromatography because the gel effectively allows for the filtering of molecules from a sample based on molecular size. However, unlike other methods, the larger molecules elute first.
Size-exclusion chromatography (SEC), also understood as molecular sieve chromatography, exists a chromatographic procedure in which molecules in solution exist separated by their size, and in some cases molecular weight. It exists usually applied to large molecules or macro molecular complexes such as proteins and industrial polymers.
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Related Questions
what are the difference between DNA and RNA
Answers
Answer:
There are two differences that distinguish DNA from RNA: (a) RNA contains the sugar ribose, while DNA contains the slightly different sugar deoxyribose (a type of ribose that lacks one oxygen atom), and (b) RNA has the nucleobase uracil while DNA contains thymine.
*Part 1*:
Interpretation - Identify trends, compare and contrast, draw conclusions.
The following is a plot of the number of bacterial cells vs time in a culture. A culture refers to
cells grown in a man-made environment. For example, even though oceans, streams and
ponds are teaming with microbes, they are not considered a culture because they are part of a
natural environment. Yogurt as well as cells grown on agar plates or liquid cultures are
examples of a man-made environment.
c.
b.
d.
a.
5
Time (hr)
10
*Part 1*: Analyze the image. Be sure to include a conclusion. There are no incorrect
conclusions, only illogical ones.
Answer:
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Answer:
First increases, attain highest population and then decreases.
Explanation:
In the culture, the number of bacterial cells increases with the passage of time in a culture. at the beginning, the bacterial cells increases in number or population with the passage of time. At point C, it shows highest population of bacterial cells at the time period of 4 to 5 hours but after more time passed, the population of bacterial cells decreases may be the unavailability of food substances in the medium.
Which of the following events contributes to the termination of a signal generated by the binding of a ligand to a receptor tyrosine kinase? Only one answer is correct
1. Phosphatases hydrolyze key phosphorylated residues.
2. Kinases hydrolyze key phosphorylated residues.
3. Kinases phosphorylate key residues.
4. The receptor tyrosine kinase dimerizes.
5. GTPase removes a phosphoryl group from GTP.
6. Phosphatases phosphorylate key residues.
Answers
Answer:
1. Phosphatases hydrolyze key phosphorylated residues.
Explanation:
Receptor Tyrosine Kinases (RTKs) are high-affinity transmembrane protein receptors that bind to a wide variety of ligands (e.g., growth factors, cytokines, hormones). These receptors (RTKs) have a transmembrane domain and therefore these proteins act as membrane receptors, as well as exhibit catalytic activity. The intracellular C terminal region of RTKs contains catalytic domains responsible for both autophosphorylation and tyrosine phosphorylation of their protein substrates. Moreover, Protein Tyrosine Phosphatase (PTPs) are critical enzymes that remove phosphate groups from tyrosine residues in different substrates (including RTKs), thereby regulating key signaling pathways such as cell proliferation, cell differentiation, and cell-cell adhesion.
In these imprinted cells, the SNRPN transcript overlaps with another gene, called UBE3A, which is transcribed in the opposite direction. This means that the region that the RNA polymerase transcribes in these two genes actually overlaps; in some cases, overlapping transcription in opposite directions leads to transcriptional interference, meaning that collisions between the polymerases traveling in opposite directions can interfere with transcription. It is believed that this takes place in this case, meaning that the more SNRPN transcription occurs, the more this disrupts transcription of UBE3A. Based on this information as well as information about the effects of imprinting on SNRPN transcription, which of the following statements about UBE3A transcription would be true?
a. The maternal copy of UBE3A is expressed and the paternal copy is silenced.
b. The paternal copy of UBE3A is expressed and the maternal copy is silenced.
c. Both copies of UBE3A are highly expressed.
d. Both copies of UBE3A are silenced.
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Answer:
a. The maternal copy of UBE3A is expressed and the paternal copy is silenced.
Explanation:
When UBE3A chromosome is in neurons, paternal allele is silent and maternal allele is expressed in process of genomic imprinting. In some cases UBE3A is expressed from both paternal and maternal chromosomes. The paternal chromosome is blue whereas maternal chromosome is magenta.
Using sophisticated molecular cloning techniques, you have isolated the genes for two serotonin transporters, called TransA and TransB, which are expressed in these neuronal cells. A preliminary immunofluores- cence localization of fixed and permeabilized cells found very bright staining of TransA at the nerve cell plasma membrane and little staining elsewhere. TransB, in contrast, stained only very faintly at the nerve cell plasma membrane, but there was intense staining within the cytosol.
Required:
In which subcellular membrane or organelle would you expect most of the TransB to be found? Explain your answer.
Answers
Answer:
Vesicles
Explanation:
Serotonin is a neurotransmitter that transports signals or messages between neurons.
Most neuronal cells have vesicles in their interior, which are organelles that store neurotransmitters for exportation -by exocytosis- or from recycling -by endocytosis-. These vesicles also protect the neurotransmitter from the enzymatic action.
Vesicles form in the cellular soma, from where they are transported to nervous terminals. Once the vesicle releases the neurotransmitter to the intercellular space, their membrane remains available in the plasmatic membrane to be reused.
The neurotransmitter concentration in the vesicle interior is related to the storage system and the transport system. There are specialized transporter proteins in the vesicle membrane that are involved with the introduction of the molecule to the organelle.
In the exposed example, transporter Trans B might be located in the vesicle membranes, and hence could be found in the cytosol of the cells.
Which actions could be categorized in the “aerobic” section of the Venn diagram?
Answers
Answer:
Starts process with a glucose molecule (Obtaining ATP begins with a breakdown of a glucose molecule (hence the name of glycose), and this is in common with the anaerobic process since this step does not require oxygen.)
Explanation:
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Answer:
If your doing the test on edgen then the answers are A.)consists of three stages, B.)yields 36 ATP molecules and, E.)starts process with a glucose molecule
Explanation:
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Na + Cl2 --> NaCl if you were given 15 grams of sodium (with excess chlorine) and asked to determine the amount of NaCl that would be produced (in grams), how would you go about doing it. Be as specific as possible and include numerical values when necessary.
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Answer:
The correct answer is - 38.15 gm of NaCl.
Explanation:
Write the balanced equation for this reaction of sodium (Na) and chlorine (Cl₂) to produce sodium chloride (NaCl):
2Na + Cl₂ —> 2NaCl
the mass of Na and the mass of NaCl :
Molar mass of Na = 23 g/mol
In the balanced equation = 2 × 23 = 46 g
Molar mass of NaCl = 23 + 35.5
= 58.5 g/mol
similarly in balanced equation = 2 × 58.5 = 117 g
From the balanced equation above,
46 g of Na reacted to produce 117 g of NaCl.
By converting it to 15 grams of Na.
Therefore,15 g of Na will react to produce = (15 × 117)/46 = 38.15 g of NaCl.
Thus, 38.15 g of Na
que contiene el condón?
Answers
Answer:
plss translate it in English so i Can easyly answer it.
Explanation:
Thank you.
Answer this question properly
Answers
Answer:
Hindi ko alam heheeExplanation:
ssorry poIn your own words, explain how the precise timing of an element's radioactive decay helps scientists find the actual age of a material? (5 points)
Answers
Answer:
Precise time can be studied with the help of amount of carbon in the body. By measuring the amount of carbon in the body scientist can find the actual age of a material
Explain the benefit of higher myoglobin levels for individuals living at high altitudes
Answers
Use the Hide Others button to hide the other structures in the view and observe the routes of the arteries. Select the largest artery, traveling from the heart downward through the torso. What is this artery called
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Answer:
The correct answer is - Aorta or descending aorta.
Explanation:
The main artery carries blood from the left ventricle of the heart to the rest of the body and going downwards in the torso is the aorta. The blood leaves the heart through the aortic valve making a cane-shaped curve that allows other major arteries to deliver oxygen-rich blood to the brain, muscles, and other cells. It is known as the largest artery of the human body.
The correct answer is - Aorta or descending aorta.
Congestive heart failure (CHF) is an inability of the heart to pump sufficient blood to the body. One sign of CHF is excess fluid in the tissue spaces, known as edema. Describe the location of the edema if the left side of the heart fails, compared to the location of edema if the right side of the heart fails.
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Answer:
Explanation:
When the left side of the heart (left ventricle) starts to fail, fluid collects in the lungs (edema). This extra fluid in the lungs (pulmonary congestion) makes it more difficult for the airways to expand as a person inhales. Breathing becomes more difficult and the person may feel short of breath, particularly with activity or when lying down.
When the right side of the heart (right ventricle) starts to fail, fluid begins to collect in the feet and lower legs. Puffy leg swelling (edema) is a sign of right heart failure, especially if the edema is pitting edema. With pitting edema, a finger pressed on the swollen leg leaves an imprint. Non-pitting edema is not caused by heart failure.
What is silk thread?
Answers
1. A biologist studying interactions between an
animal species and its environment is studying
biology at which level?
b. biosphere
c. organism
d. ecosystem
a. cell
Answers
Answer:
ecosystem
Explanation:
That is the definition of ecology.
When a neuron is stimulated and sodium channels open, the process of has begun. Structures of the nervous system surrounding, but not part of the central nervous system are part of the . Two consecutive neurons communicate at a , where they physically come together. are a group of natural chemicals that reduce the perception of pain. Supporting cells of the nervous system are collectively called . When potassium channels have been open long enough to go below resting membrane potential, has occurred.
Answers
Answer:
- When a neuron is stimulated and sodium channels open, the process of depolarization has begun.
- Structures of the nervous system surrounding, but not part of the central nervous system are part of the peripheral nervous system
- Two consecutive neurons communicate at a synapse, where they physically come together.
- Endorphins are a group of natural chemicals that reduce the perception of pain.
- Supporting cells of the nervous system are collectively called neuroglia
- When potassium channels have been open long enough to go below resting membrane potential, hyperpolarization has occurred.
Explanation:
Neurons undergo depolarization in response to diverse stimuli (e.g., heat, light, electrical, chemical, etc). The depolarization occurs when sodium (Na+) ions pass through specific channels (i.e., open voltage-gated Na+ channels) into a neuron, which causes an increase in the positivity of membrane potential. On the other hand, hyperpolarization occurs when potassium (K+) channels remain open and Na+ channels reset, thereby producing an increase in negativity of membrane potential. The peripheral nervous system (PNS) is composed of the cranial nerves, spinal nerves, peripheral nerves, and neuromuscular junctions, which connect the Central nervous system (CNS) to the organs, limbs, and skin. Endorphins (e.g., serotonin) are polypeptides secreted by the pituitary gland and CNS to relieve stress and pain. Neuroglia refers to a class of neural cells that have ectodermal (e.g., astroglia, peripheral glial cells) or mesodermal (e.g., microglia) origins. Neuroglia cells are involved in diverse roles, including, among others, the formation of the blood-brain barrier, homeostatic support, provision of nutrients for neurons, structural support to nerve cells, etc.
what happens to the respiratory system of a pilot who is in a power dive in supersonic plane.
no links
Answers
Explanation:
Due to lack of O2, the lungs will start breathing anaerobically i.e; without oxygen this may lead to cramps
Answer:
Due to lack of O2, the lungs will start breathing anaerobically
i.e.; without oxygen. This may lead to cramps.
Which of the following is a benefit of dams?
A. They impede fish migration and spawning
B. They provide a source of energy
C. They increase the sediment load of waterways
D. They divert water from ecosystems
Answers
What is the purpose of a geological time scale ?
It used to predict natural disaters throughout Earth’s history.
It is used to present the correct sequence of events in the Earth’s history.
It is used to determine the absolute dates in years for different periods.
It used to create a naming system for flora and fauna.
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Answer: B. It is used to present the correct sequence of events in the Earth’s history.
Explanation: On Edge!!!! :)
Answer:bbbbb
Explanation:
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A solid reactant is placed into a beaker of a warm water. The liquid vigorously bubbles as the solid dissolves into the solution. What will most likely happen if the temperature of the liquid is slightly reduced?
More bubbles will be produced because the solution is becoming more concentrated.
Fewer bubbles will be produced because of fewer collisions of reactant molecules.
The solid will get smaller at a faster rate because of more collisions of reactant molecules.
The solid will get larger at a slower rate because precipitate is coming out of the solution.
Answers
Answer: Fewer bubbles will be produced because of fewer collisions of reactant molecules.
Answer:
B) Fewer bubbles will be produced because of fewer collisions of reactant molecules.
Explanation:
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7. The sun's surface is about
a. 2000 °C
b. 3000 °C
c. 4000 °C
d. 5000 °C
(;´༎ຶٹ༎ຶ`)
Answers
Answer:
5778C ...................,.....
A white colony growing on mannitol salt agar tests negative for coagulase and novobiocin sensitivity. This bacterium is most likely ________________
A white colony growing on mannitol salt agar tests negative for coagulase and novobiocin sensitivity. This bacterium is most likely ________________
Streptococcus pyogenes.
Staphylococcus epidermidis.
Stpahylococcus saprophyticus.
Staphylococcus aureus.
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Answer:
for the first it is Staphylococcus saprophyticus. and for the second it is Streptococcus pyogenes.
Explanation:
Answer:
1. Staphylococcus aureus.
2. Staphylococcus epidermidis.
Explanation:
define cell and atom.....
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Cell: A cell is the structural and fundamental unit of life. The study of cells from its basic structure to the functions of every cell organelle is called Cell Biology.
Atom: atom is the smallest component of an element, characterized by a sharing of the chemical properties of the element and a nucleus with neutrons, protons and electrons. The protons and the neutrons reside in the nucleus.
whats the difference between atom and cell?
function wise atoms take part in every chemical reaction while cells are responsible for the development and growth of living existences. Atoms do not have life. They do not need food, water, and they do not reproduce. Cells are alive. Cells consume food and water and can reproduce. Atoms construct molecules and Cells make tissues for organs.
P is an autosomal dominant allele that produces a pigment, p is a recessive allele that produces no pigment. A pigmented and a non-pigmented organism mate and produce a pigmented offspring. They then mate and produce a non-pigmented offspring. What is the probability the next offspring will be pigmented
Answers
Answer:3/4
Explanation:4 possibilities, 3 dominant
If a pigmented and non-pigmented organism mate and produce a pigmented organism, which mate again and produce non-pigmented organisms, the probability that the next offspring will be pigmented is ¾ as P is the dominant, pigment-producing allele.
What are dominant alleles?The association between two genetic variants is referred to as dominant. Each gene has two alleles that an individual inherits from each parent. One allele of a gene, known as the dominant gene, will be expressed if the alleles are different. The impact of the additional allele, known as the recessive one, is concealed.
If one is in charge, the other must be recessive. Then it is referred to as recessive. A dominant gene, or dominant form of a gene, is a specific variant of a gene that expresses itself more powerfully on its own than any other version of the gene that the individual is carrying—in this example, the recessive—for a variety of reasons.
Now, it mainly refers to patterns of inheritance typically used in combination with a Punnett square, where if a person has two copies of a gene and one is shown to frequently be passed down from one generation to another, it is referred to as dominant.
According to biochemistry, what is happening in this situation is that the genetic variant may, for a variety of reasons, cause a cell to perform a very advantageous or very disadvantageous function that the other version of the gene cannot cover up or counteract.
Therefore, the probability that the next offspring will be pigmented is ¾ as P is the dominant, pigment-producing allele.
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Hemophilia is an X-linked recessive condition in which blood does not clot properly. Queen Victoria of England had one allele for hemophilia. Most of her male descendants had the disorder, but few females had it. Why did hemophilia occur more frequently in Queen Victoria’s male descendants?
Answers
Answer:
Because hemophilia is an X-linked recessive condition.
Explanation:
As the problem states, hemophilia is an X-linked recessive condition.
Meaning that in Queen Victoria's female descendants (coded XX) which inherited the Queen's one allele, for hemophilia to appear in them it would be necessary to inherit another hemophillia-carrying allele from the father.The Queen's male descendants (coded XY) would always manifest hemophilia if they inherited the Queen's hemophilia allele, as they possesed only one X gene.what can happen during the cycle of photosynthesis with increasing concentrations of Atrazine?
Answers
Answer:
By inhibiting photosynthesis, Atrazine kills plants. Photosynthesis, which is unique to living green plants, occurs when light energy becomes the chemical energy required for food production.
Simply stated, plants eventually die of hunger when food production stops. Atrazine selectively kills unwanted plants (weeds) competing with suitable plants for water and nutrients (crops, turfgrass). It is thus a beneficial tool for both farming and urban plants.
Explanation:
In Texas maize and grain sorghum production, Atrazine is the most often used herbicide. In several "weed and feed" preparations for domestic plowing and commercial turf-grass weed control, it is also often found as an active ingredient.
With its broad use, atrazine has been identified in minute concentrations in Texas ground and surface waterways. Because of this, it is crucial to understand atrazine behavior in the environment and the potential for harmful impacts on human and animal health and on other non-target organisms.
Using the organisms you identified in part B, create a food web for the ecosystem you chose. Use this sample food web for reference, although your food web will contain fewer organisms. Note that your food web does not have to include images, but you may include them if you choose. However, be sure to include arrows to indicate the direction of energy flow in your food web. Design your food web using any method listed below:
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Unfortunately you did not introduce the organisms identified in part B, as the question shows. This makes it impossible for me to answer this question, but I will try and help as best I can.
In the figure attached to the question below, it is possible to see an example of a food web and the arrows that indicate the direction of the energy flow within this food web.
In this sense, we can determine that a food web is the set of food chains that complete and mix. A food chain shows how living organisms organize themselves so that one feeds on the other, whereas in the food web, this food chain is shown in a more realistic way, as it shows how food chains mix and interact within the ecosystem, as they do not there are food chains isolated in nature.
Answer:
I did Lake Tahoe. Feel free to use this image!
Explanation:
The scientists mapping the SNPs in the human genome noticed that groups of SNPs tended to be inherited together, in blocks known as haplotypes, ranging in length from 5,000 to 200,000 base pairs. There are as few as four or five commonly occurring combinations of SNPs per haplotype. Integrating what you've learned throughout this chapter and this unit, propose an explanation for this observation.
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Answer:
SNPs have shown that only 0.1 % of DNA sequences are different in the human genome between different individuals, thereby all the inherited phenotypic variation observed in our species is associated with only 0.1 % of differences at the genome level
Explanation:
Haplotypes are block-like sequences of DNA that are inherited together due to low recombination rates. Moreover, single-nucleotide polymorphism (SNP) mapping is a very useful methodology used to map the site of SNP mutations (i.e., SNP variants). In this regard, it has been observed that there are approximately 10 million common SNPs in the human genome. These SNPs contribute to the wide range of phenotypic variation observed in human populations for different traits (e.g., eye color, hair, weight, height, etc). Moreover, researchers have determined that SNPs can be clustered into haplotypes, thereby haplotypes can be accurately sampled by as few as approx. 300,000 selected SNPs, which are sufficient to represent all of the genetic variation across different human genomes.
All claims in science should be supported by biology
Answers
Answer:
yes
Explanation:
Because science &biology have a connection
Mutations that result in the death of the individual are known as
Answers
Answer:
Mutations that cause the death of an organism are called lethals — and it doesn't get more negative than that.
