You have selected your two primers and added all of the PCR components to a test tube. Use the answer choices [A-C] to explain what will happen as the polymerase chain reaction proceeds. Answers may be used more than once or not at all.
Hi. You did not provide any response options. However, a PCR reaction proceeds as follows.
After the primers are added to the test tube containing the PCR components. This tube is placed in a device called a thermocycler. At that moment, the stage called denaturation will begin, where the thermocycler increases the temperature to the point of breaking the hydrogen bonds that hold the two strands of DNA together. The thermal cycler increases the temperature up to 96°C.
After that, the second step of the reaction begins. At that moment, the thermal cycler lowers the temperature to 55º - 65ºC, which is the ideal temperature for the primers to be able to attach themselves to the DNA strands, preparing them for the presence of the polymerase.
After that, the thermocycler raises the temperature to 72ºC, which is the ideal temperature for the DNA polymerase to work. At this stage, the DNA polymerase will use the DNA strand and the primer to build a new DNA strand, which will be annealed to the DNA strand used as a template.
These three steps will be repeated about 35 times, generating many copies of DNA.
What is the unite of Kc ?
Answer:
There is no unit for Kc
Explanation:
Please Help !! This is an Earth science lab question.
Explain why erosion occurs on the outside of a meander and deposition on the inside of a meander.
Answer:
The sideways movement occurs because the maximum velocity of the stream shifts toward the outside of the bend, causing erosion of the outer bank. At the same time the reduced current at the inside of the meander results in the deposition of coarse sediment, especially sand
Your Turn!
How many electrons are required to complete
the octet around nitrogen, when it forms N2
?
A. 2
B. 3
C. 1
D. 4
E. 6
Answer:
because.......
...........
.........
ASAP!!!!! The data table below shows a person’s heart rate measured in beats per minute (bpm) at five different times in the beginning of a day. What causes the change in heart rate over time?
1. tired
2. increased activity
3. passing of time
4. forgetting to eat
Which of the following has the highest pH?
A. 0.01 M HCI
B. 0.1 M HCI
C. 1 M HCI
D. 0.001 M HCI
Answer:
D
Explanation:
pH=-log(x)
x=0.001M,pH=3
x=0.01M,pH=2
x=0.1M,pH=1
x=1M,pH=0
Highest pH is for option D
Answer:
D.
Explanation:
The highest pH is D because
0.01 M HCL => 2
0.1 M HCL => 1
1 M HCL => 0
0.001 M HCL => 3
so the answer is D.
What other reactions is taking place?
write the formula two atom of iron and three atoms of oxygen
Answer:
Fe2O3 is the formula this is your correct answer
50.00 mL of unknown calcium hydroxide solution is titrated with 0.250 M standard nitric acid solution. If 43.43 mL of the standard acid solution is required to reach a phenolphthalein endpoint, what is the molarity of the unknown calcium hydroxide solution
Answer: Molarity of the unknown calcium hydroxide solution is 0.217 M.
Explanation:
Given: [tex]V_{1}[/tex] = 50.00 mL, [tex]M_{1}[/tex] = ?
[tex]V_{2}[/tex] = 43.43 mL, [tex]M_{2}[/tex] = 0.250 M
Formula used is as follows.
[tex]M_{1}V_{1} = M_{2}V_{2}[/tex]
Substitute the values into above formula as follows.
[tex]M_{1}V_{1} = M_{2}V_{2}\\M_{1} \times 50.00 mL = 0.250 M \times 43.43 mL\\M_{1} = \frac{0.250 M \times 43.43 mL}{50.00 mL}\\= 0.217 M[/tex]
Thus, we can conclude that molarity of the unknown calcium hydroxide solution is 0.217 M.
What is the pressure, in atm, exerted by 41.6 g oxygen in a 22.0L container at 30.0C ?
Answer:
1.47 atm
Explanation:
Step 1: Calculate the moles corresponding to 41.6 g of oxygen
The molar mass of oxygen is 32.00 g/mol.
41.6 g × 1 mol/32.00 g = 1.30 mol
Step 2: Convert 30.0 °C to Kelvin
We will use the following expression.
K = °C + 273.15 = 30.0 + 273.15 = 303.2 K
Step 3: Calculate the pressure exerted by the oxygen
We will use the ideal gas equation.
P × V = n × R × T
P = n × R × T / V
P = 1.30 mol × (0.0821 atm.L/mol.L) × 303.2 K / 22.0 L = 1.47 atm
An atom has the electron configuration of 1s22s22p5 how many electrons are in that atom
Answer:
There are [tex]9[/tex] electrons in this atom.
Explanation:
Electron configuration of this atom: [tex]1s^2\, 2s^2\, 2p^5[/tex].
The electron orbitals of an atom are denoted as [tex]1s[/tex], [tex]2s[/tex], [tex]2p[/tex], [tex]3s[/tex], [tex]3p[/tex], etc. At any given time, an electron in this atom is located in exactly one orbital.
The electron configuration of an atom gives the number of electrons in each orbitals of this atom.
For example, in this atom, the superscript "[tex]2[/tex]" on the right of "[tex]1s[/tex]" means that there are two electrons in the [tex]1s\![/tex] orbital of this atom. Hence, [tex]1s^2\, 2s^2\, 2p^5[/tex] would translate to:
The [tex]1s[/tex] orbital of this atom contains [tex]2[/tex] electrons.The [tex]2s[/tex] orbital of this atom contains [tex]2[/tex] electrons.The [tex]2p[/tex] orbitals of this atom contain [tex]5[/tex] electrons.Hence, there would be [tex]2 + 2 + 5 = 9[/tex] electrons in total in this atom.
What is the total number of atoms in one formula unit of MgSO4.7H2O?
Answer:
11.
O4 means 4 atoms of oxygen. H2O
has one atom of oxygen, so seven "units" of
H2O has 7.
When the nuclide polonium-218 undergoes alpha decay:
a. The name of the product nuclide is _____.
b. The symbol for the product nuclide is _____.
Write a balanced nuclear equation for the following: The nuclide polonium-218 undergoes alpha emission.
Answer:
a): The name of the product nuclide is lead-214
b): The symbol of the product nuclide is Pb-218
Explanation:
There are three types of decay processes:
Alpha decayBeta decayGamma decayAlpha decay is the decay process that happens when a heavy nucleus decays into a light nucleus with the release of an alpha particle. This alpha particle carries a charge of +2 units and has a mass of 4 units. It is also known as the helium nucleus. The general equation for this decay process is:
[tex]_Z^A\textrm{X} → _{Z-2}^{A-4}\textrm{Y}+_2^4\alpha[/tex]
The nuclear equation for the alpha decay of Po-218 follows:
[tex]_{84}^{218}\textrm{Po}\rightarrow _{82}^{214}\textrm{Pb}+_2^4\alpha[/tex]
Hence, the name of the product nuclide is lead-214 and the symbol is Pb-218.
sino may kuyang palaging nambibira
An unknown element, X, has an atomic mass of 107.868 amu. The X-109 isotope (108.905 amu) is 48.16%. What is the amu of the other isotope (report final answer to the correct number of significant figures)
Answer:
106.905 amu is the mass of the other isotope
Explanation:
The atomic mass of an element is the sum of the masses of the isotopes multiplied by its abundance. The atomic mass of an element X with 2 isotopes is:
X = X-109*i + X-107*i
Where X is the atomic mass = 107.868 amu
X-109 = 108.905amu, i = 48.16% = 0.4816
X-107 = ?, i = 1-0.4816 = 0.5184
Replacing:
107.868amu = 108.905amu*0.4816 + X-107*0.5184
55.4194 = X-107*0.5184
106.905 = X-107
106.905 amu is the mass of the other isotopeGive two ways to make salt conduct.
Answer:
I think u willl get the answer fast in......
Answer:
this is because when a salt dissolves, its dissociated ions can move freely in solution, allowing a charge to flow. The resulting solution will conduct electricity because it contains ions.
Explanation:
9.0+ 2.000 (35.35) =
Answer:
79.7
Explanation:
Answer:79.7
Explanation:just got it right on the quiz!
This week's imide synthesis involves two reactions. In the first reaction (24A), a(n) ________ bond is formed between the two reactants. Hint: What type of functional group is formed
Answer:
C - N Bond formation.
Explanation:
Imide synthesis is a chemical reaction in organic chemistry which consists of two acyl groups which bond to nitrogen atom. The compound structure is related to acid anhydrides. Imides are monoacyl which are used as valuable intermediates in organic synthesis.
Calculate the amount of energy produced by the conversion of 50.0 kg of mass into energy. Use 3.00 x 108 m/s for the speed of light. Round to 3 significant digits.
Which setup will solve this problem?
Answer:
tanong mo sa teacher mo ok
Answer:
E = (50.0 kg)(3.00 x 108 m/s)2
Explanation:
Determine the empirical formula of a compound having the following percent composition by mass: K: 24.74%; Mn: 34.76%; O: 40.50%
Answer: The empirical formula of the compound is [tex]KMnO_4[/tex]
Explanation:
The empirical formula is the chemical formula of the simplest ratio of the number of atoms of each element present in a compound.
The number of moles is defined as the ratio of the mass of a substance to its molar mass. The equation used is:
[tex]\text{Number of moles}=\frac{\text{Given mass}}{\text{Molar mass}}[/tex] ......(1)
Let the mass of the compound be 100 g
Given values:
% of K = 24.7%
% of Mn = 34.76%
% of O = 40.50%
Mass of K = 24.7 g
Mass of Mn = 34.76 g
Mass of O = 40.50 g
To calculate the empirical formula of a compound, few steps need to be followed:
Step 1: Calculating the number of moles of each elementWe know:
Molar mass of K = 39.10 g/mol
Molar mass of Mn = 54.94 g/mol
Molar mass of O = 16 g/mol
Putting values in equation 1, we get:
[tex]\text{Moles of K}=\frac{24.7g}{39.10g/mol}=0.632 mol[/tex]
[tex]\text{Moles of Mn}=\frac{34.76g}{54.94g/mol}=0.633 mol[/tex]
[tex]\text{Moles of O}=\frac{40.50g}{16g/mol}=2.53 mol[/tex]
Step 2: Calculating the mole fraction of each element by dividing the calculated moles by the least calculated number of moles that is 0.632 moles[tex]\text{Mole fraction of K}=\frac{0.632}{0.632}=1[/tex]
[tex]\text{Mole fraction of Mn}=\frac{0.633}{0.632}=1[/tex]
[tex]\text{Mole fraction of O}=\frac{2.53}{0.632}=4[/tex]
Step 3: Writing the mole fraction as the subscripts of each of the elementThe empirical formula of the compound becomes [tex]K_1Mn_1O_4=KMnO_4[/tex]
Hence, the empirical formula of the compound is [tex]KMnO_4[/tex]
The empirical formula of the compound is KMnO₄
Data obtained from the question Potassium (K) = 24.74%Manganese (Mn) = 34.76%Oxygen (O) = 40.50%Empirical formula =? How to determine the empirical formulaDivide by their molar mass
K = 24.74 / 39 = 0.634
Mn = 34.76 / 55 = 0.632
O = 40.50 / 16 = 2.531
Divide by the smallest
K = 0.634 / 0.632 = 1
Mn = 0.632 / 0.632 = 1
O = 2.531 / 0.632 = 4
Therefore, the empirical formula of the compound is KMnO₄
Learn more about empirical formula:
https://brainly.com/question/24297883
What is always true of a salt
(a) shows both acidic and basic properties
(b) creates a neutral pH in solution
(c) is an ionic compound formed from an acid-base reaction
(d) can react with acid but not with a base
FULL FORM OF NASA??
lol
Answer:
NASA stands for National Aeronautics and Space Administration
Answer:
National Aeronautics and Space Administration
hope this will help you more
Which statement best describes the intermolecular forces between H2
molecules and NH3 molecules in the liquid phase?
A. Dipole-dipole forces are the strongest force between H2
molecules, and Van der Waals forces are the strongest force
between NH3 molecules.
B. Van der Waals forces are the strongest force between H2
molecules, and hydrogen bonding is the strongest force between
the NH3 molecules.
C. Hydrogen bonding is the strongest force between H, molecules
and between NH3 molecules.
D. Van der Waals forces are the strongest force between H2
molecules and between NH3 molecules.
Answer:
D. Van der Waals forces are the strongest force between H2
molecules and between NH3 molecules.
Explanation:
Van der Waal’s forces are the forces which arises due to disturbance in the electron density of the molecule.
These are usually found in non polar molecules. Hence N2 is said to exhibit this force.
Out of these Van der Waals is the weakest force.
A buffer solution contains 0.475 M nitrous acid and 0.302 M sodium nitrite . If 0.0224 moles of potassium hydroxide are added to 150 mL of this buffer, what is the pH of the resulting solution
Answer: The pH of the resulting solution will be 3.001
Explanation:
Molarity is calculated by using the equation:
[tex]\text{Molarity}=\frac{\text{Moles}}{\text{Volume}}[/tex] ......(1)
We are given:
Moles of NaOH = 0.0224 moles
Molarity of nitrous acid = 0.475 M
Molarity of sodium nitrite = 0.302 M
Volume of solution = 150 mL = 0.150 L (Conversion factor: 1 L = 1000 mL)
Putting values in equation 1, we get:
[tex]\text{Moles of nitrous acid}=(0.475mol/L\times 0.150L)=0.07125mol[/tex]
[tex]\text{Moles of sodium nitrite}=(0.302mol/L\times 0.150L)=0.0453mol[/tex]
The chemical equation for the reaction of nitrous acid and NaOH follows:
[tex]HNO_2+NaOH\rightleftharpoons NaNO_2+H_2O[/tex]
I: 0.07125 0.0224 0.0453
C: -0.0224 -0.0224 +0.0224
E: 0.04885 - 0.0677
The power of the acid dissociation constant is the negative logarithm of the acid dissociation constant. The equation used is:
[tex]pK_a=-\log K_a[/tex] ......(2)
We know:
[tex]K_a[/tex] for nitrous acid = [tex]7.2\times 10^{-4}[/tex]
Using equation 2:
[tex]pK_a=-\log (7.2\times 10^{-4})=3.143[/tex]
To calculate the pH of the acidic buffer, the equation for Henderson-Hasselbalch is used:
[tex]pH=pK_a+ \log \frac{\text{[conjugate base]}}{\text{[acid]}}[/tex] .......(3)
Given values:
[tex][NaNO_2]=\frac{0.0677}{0.150}[/tex]
[tex][HNO_2]=\frac{0.04885}{0.150}[/tex]
[tex]pK_a=3.143[/tex]
Putting values in equation 3. we get:
[tex]pH=3.143-\log \frac{(0.0677/0.150)}{(0.04885/0.150)}\\\\pH=3.143-0.142\\\\pH=3.001[/tex]
Hence, the pH of the resulting solution will be 3.001
Calcula la concentración de H+ de una sustancia que tiene pH 8.8
Answer:
[tex]pH = - log[H {}^{ + } ] \\ 8.8 = - log[H {}^{ + } ] \\ \: [H {}^{ + } ] = {10}^{ - 8.8} \\ [H {}^{ + } ] = 1.585 \times {10}^{ - 9} \: mol {dm}^{ - 3} [/tex]
The energy released by a nuclear fusion reaction is produced when
A.
energy is converted to mass
B.
mass is converted to energy
C.
heat is converted to temperature
D.
temperature is converted to heat
The nuclear fusion results in the liberation of large amount of energy by the conversion of mass to energy. Thus, option B is correct.
The nuclear fusion has been a type of nuclear reaction in which the reaction of the two nuclei results in the nuclei with mass smaller than the reactants. In nuclear reactions, there has been the liberation of large amount of energy.
The energy released in the nuclear fusion has been formed by the conversion of the mass. Since, the formed product has nuclei with the mass smaller than the reactants, the remaining mass of the reactants has been converted to the energy.
Thus, in nuclear fusion, the energy released has been produced from the conversion of mass to energy. Thus, option B is correct.
For more information about the nuclear fusion, refer to the link:
https://brainly.com/question/16021285
Nitrogen gas and chlorine gas will react to form nitrogen monochloride gas. 5 moles of nitrogen and 10 moles of chlorine are mixed in a previously evacuated 2 L flask at 500 K. After equilibrium has been established, there are 3 moles of NCl (g) present. What is the equilibrium constant (Kc) for this reaction at 500 K
Answer:
0.30
Explanation:
Step 1: Calculate the known initial and equilibrium molar concentrations
[N₂]i = 5 mol/2 L = 2.5 M
[Cl₂]i = 10 mol/2 L = 5 M
[NCl]e = 3 mol/2 L = 1.5 M
Step 2: Make an ICE chart
N₂(g) + Cl₂(g) ⇄ 2 NCl(g)
I 2.5 5 0
C -x -x +2x
E 2.5-x 5-x 2x
Step 3: Find the value of x
We know that [NCl]e = 2x = 1.5 ⇒ x = 0.75 M
Step 4: Calculate the concentrations at equilibrium
[N₂]e = 2.5-x = 2.5-0.75 = 1.75 M
[Cl₂]e = 5-x = 5-0.75 = 4.25 M
[NCl]e = 1.5 M
Step 5: Calculate the equilibrium constant (Kc)
Kc = [NCl]² / [N₂] × [Cl₂]
Kc = 1.5² / 1.75 × 4.25 = 0.30
How many moles of AICI3 are produced?
Answer:
please correct it, the question is incomplete
Write the molecular formula for the compound that exhibits a molecular ion at M+ = 112.0499. Assume that C, H, N, and O might be present, and use the exact masses below: Exact mass of carbon = 12.000 Exact mass of hydrogen = 1.0078 Exact mass of nitrogen = 14.003 Exact mass of oxygen = 15.995 (The order of atoms should be carbon, then hydrogen, then the others in alphabetical order. If there is more than one answer, just give one. ) Molecular formula:
Answer:
C₅H₈N₂O
Explanation:
The molecular formula denotes the various forms of atoms contained in a molecule at a particular fixed proportion.
The molecular ion M⁺ = 112.0499
and the exact mass values are given as follows:
C = 12.000
H = 1.0078
N = 14.003
O = 15.995
By assumption:
C = 12.000 × 5 = 60.0000
H = 1.0078 × 8 = 1.0078
N = 14.003 × 2 = 28.0060
O = 15.995 × 1 = 15.9950
= 112.0634
This is approximtely equal to 112.0499.
As such the Molecular formula for the compound = C₅H₈N₂O
Make tag question.
neither of them plays cricket,......?
Answer:
neither of them plays cricket,does anyone?
Explanation:
I know you will think I its weird but it is the rule of grammar and 100% correct!
Answer:
neither of them plays cricket, do you?
Explanation:
