An acid-fast staining method was performed on a bacterial smear using the dyes carbol fuchsin and methylene blue. The resulting stained slide contained only cells that appeared blue when visualized using a microscope. Which of the following could explain why only blue cells were present?

a. No acid-fast bacteria are present on the slide
b. Methylene blue was not used
c. Carbolfuchsin was not used
d. Acid-alcohol was not used

Answers

Answer 1

Answer: Only blue cells where present because No acid-fast bacteria are present on the slide. The correct option is A.

Explanation:

Acid fast staining method also called Ziehl-Neelsen (Zn) technique is used in the laboratory to stain samples suspected to contain Mycobacterium species. The Mycobacterium species which includes the M. tuberculosis, M. ulcerans, and M. leprae, do not stain well by the Gram technique, which is a common routine stain in the laboratory. This is because of waxy coating on its cell surface primarily due to the presence of mycolic acid.

The acid fast stain consists of:

--> A primary dye which is the carbol

fuchsin

--> A decolorizer which is the acid alcohol

--> A counter stain which is the methylene blue.

In it mechanism of action on the bacterial smear, when the primary dye is applied,the whole cell will appear red. But after decolorizing with acid alcohol, only acid fast bacteria will retain the red colour of carbol

fuchsin while the red dye from the background cells are removed. Then the counterstain (methylene blue) is applied which is picked up by non-acid-fast bacteria making the cells appear blue under the microscope.

Therefore when the bacteria smear is viewed under the microscope and only blue cells are seen, it means No acid-fast bacteria are present on the slide.


Related Questions


PLEASE HELP !! ILL GIVE BRAINLIEST *EXTRA 40 POINTS* DONT SKIP :(( .!

Answers

Answer:D

Explanation:

Answer is D. Good luck. Hydropower plants capture the energy of falling water to generate electricity. Hydropower turbines kill and injure some of the fish that pass through the turbine.

What is the difference between diurnal and nocturnal?

Choose all that apply

Question 8 options:

diurnal means awake during the day, and use the light to their advantage


nocturnal active at night.


nocturnal mean awake during the day, and use the light to their advantage.


diurnal means active at night

Answers

Answer:

diurnal means awake during the day, and use the light to their advantage

nocturnal active at night.

Explanation: Diurnal means active during the day, such as a mosquito meanwhile, a nocturnal animal or being is only active during the night, and sleeps the day away, such as a bat

Hope this helps ^^

Diurnal means awake during the day time, and use the light to their advantage. Nocturnal means active at night time. Thus, the correct options will be A and B.

What is Diurnal and Nocturnal?

Based on the activeness of an organism during a day or 24 hours time period, the animals or organisms can be classified into two different types which are nocturnal and diurnal organisms. Nocturnal animals are the animals which are most active during the night time, and diurnal animals are the animals which are active during the daytime.

Diurnal animals are those animals which are awake during the day time, and use the light energy to their advantage. Nocturnal animals are the animals which are active at the night time.

Therefore, the correct options will be A and B.

Learn more about Diurnal here:

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1) You can see two moths in this image, Living on the bark of trees. They
are the same species of moth; they differ in color. If the habitat of the
two moths does not change, predict what will happen to the moth
population in this area and explain why.
A)
If the habitat does not change there should be no
change in the moth population.
B)
The lighter moth population will have better chance
of survival due to camouflage. Light moths will
increase: dark moths will decrease.
C)
The population will shift to the to favor the light
moth. The light moth population will increase
because the dark moths will leave the area.
D)
As the seasons change, both moths will be hidden by
foliage growing on the trees. We would predict no
big change in the moth populations, light or dark.

Answers

The Correct answer Is B.

this is because of natural selection

hope this helps !

The lighter moth population will have a better chance of survival due to camouflage. Light moths will increase and dark moths will decrease due to industrial melanism. Therefore, option B is correct.

What is industrial melanism?

Industrial melanism is a phenomenon in which the frequency of dark-colored or melanistic individuals in a population of organisms increases as a result of environmental pollution.

This phenomenon was first observed in the 19th century in industrialized regions of England, where the population of peppered moths shifted from mostly light-colored to mostly dark-colored in response to pollution from industrial activities.

Thus, the lighter moth population will have a better chance of survival due to camouflage. Light moths will increase and dark moths will decrease. Therefore, option B is correct.

Learn more about industrial melanism, here:

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How many hydrogen bonds are present in the base pairing of guanine and cytosine?
3

Answers

Answer:

3 Hydrogen bonds

Explanation:

Cytosine and guanine pairing can be found in both DNA and DNA-RNA hybrid formed during replication and transcription. The two nitrogenous bases are held together by three hydrogen bonds.

These weeds are growing in a farmer's field. The weeds compete with the soybean plants the farmer grows, so he needs to get rid of them. For a few years, the former relies on manually removing the woods, but that is labor intensive and limits how much crop he can grow. The farmer decided to change his crop to genetically modified soybeans that are herbicide resistant. This allows the farmer to use chemicals to remove the weeds. With this GM soybean, the chemical kills the woods but does not harm the crop. For many years this works, and allows the farmer to expand his farm and grow more soybeans. But then, one of the farmer's fields has a large weed plant remaining after the field has been sprayed with herbicide
1. Which of the following is the likely reason the weed has remained?
a. The weed needed to be resistant in order to survive, so it became resistant
b. A mutation appeared in one weed plant that made that weed not susceptible to the herbicide
2. Which of the following is likely to happen if that weed stays in place?
a. The trait will spread to nearby weeds
b. The weed will survive long enough to reproduce
c. In time, the herbicide will no longer be effective on that field.

Answers

Answer:

1) A mutation appeared in one weed plant that made that weed not   susceptible to the herbicide ( B )

2) The weed will survive long enough to reproduce ( B )

Explanation:

1) The most likely reason the weed remained is :  A mutation appeared in one weed plant that made that weed not susceptible to the herbicide

The weed plant must have undergone some mutation in order to be resistant to the herbicide which would kill the weed before now

2) The most likely thing that will happen if the weed stays in place in that farm is : The weed will survive long enough to reproduce

The trait or mutation of the weed cannot just spread to other weeds nearby it can only spread by reproducing more weeds of same mutation

5. A pride of lions hunting a dazzle of zebras.

Answers

Answer:

lions hunting zebras is a part of nature

Explanation:

What is the major distinction scientists use to divide the animal kingdom?

Answers

Answer:

Whether the animal has a vertebral column or not.

A major cause of genetic variation within a species is the result of -

Answers

Mutation, recombination and immigration of genes

The ability to taste PTC comes from the presence of a dominant allele (T). A population of 20 individuals was surveyed to determine whether or not they could taste PTC. Sixteen out of the twenty surveyed could taste PTC. Four could not taste PTC. Assume that Hardy-Weinberg Equilibrium conditions are met in the population. Round all answers properly to 2 decimal digits. Rounding rules: When rounding to 2 decimal digits, look at the third decimal digit. If the third digit is 5 or greater, round up. If the third digit is less than 5, round down.
What is the frequency of the non-taster allele (t) in this population?
What is the frequency of the taster allele (T) in this population?
What is the frequency of the TT genotype? Answer What is the frequency of the Tt genotype?
What is the frequency of the tt genotype? Answer How many people in this population are expected to be heterozygous for this locus? Round to the closest whole number.

Answers

Answer:

1) What is the frequency of the non-taster allele (t) in this population?  

f (t) = q = 0.45

2) What is the frequency of the taster allele (T) in this population?

f (T) = p = 0.55

3) What is the frequency of the TT genotype?

F (TT) = p² = 0.3

4) What is the frequency of the Tt genotype?

F (Tt) = 2pq = 0.5

5) What is the frequency of the tt genotype?

F (tt) = q² = 0.2  

6) How many people in this population are expected to be heterozygous for this locus?

Tt = 10 individuals

Explanation:

Due to technical problems, you will find the complete explanation in the attached files

1) What is the frequency of the non-taster allele (t) in this population?  

t = q = 0.45

2) What is the frequency of the taster allele (T) in this population?

T= p = 0.55

3) What is the frequency of the TT genotype?

TT = p² = 0.3

4) What is the frequency of the Tt genotype?

Tt= 2pq = 0.5

5) What is the frequency of the tt genotype?

tt = q² = 0.2  

6) How many people in this population are expected to be heterozygous for this locus?

Tt = 10 individuals

What is a genotype?

A genotype is someone's collection of genes. The word also can guide the two alleles inherited for a respective gene.

The genotype is defined when the information encoded in the genes' DNA is used to make protein and RNA molecules.

Thus, all questions related to Hardy-Weinberg Equilibrium.

To learn more about Hardy-Weinberg Equilibrium click here:

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8. Which of the following correctly shows one path that energy would
flow through this ecosystem?
Grasses -- Termite -- Monkey -- Eagle
Hyena -- Mongoose - Mouse - Grasses
Grasses - Termite - Grasshopper -- Monkey
Acadia trees - Giraffe
-- Lion -- Vulture

Answers

grassses -- Termite -- Monkey -- Eagle

The____ of an ecosystem is based on wheather the population in the system destorys the natural resources of the system

Answers

Answer: immense

immense in the blank

please make me brainliest

What does "fishing down the food web" mean?​

Answers

Answer:

Fishing down the food web is the process whereby fisheries in a given ecosystem, "having depleted the large predatory fish on top of the food web, turn to increasingly smaller species, finally ending up with previously spurned small fish and invertebrates".

*15 points* Earth Science
Based on the picture, how many tilting events has this area undergone, and which principle tells us that this area has undergone tilting?

Answers

Just saying it says 8 points ya little baka

Why should everyone be encouraged to take an HIV blood test?​

Answers

to slow down and prevent it's spread

Explanation:

testing is the only way to know for sure if you have HIV. Knowing your status means you keep yourself and your sexual partners healthy.

PLS HELP ILL GIVE YOU BRAINLEIST PLS PLS PLS PLS PLS PSL

Answers

Answer:

on what question should i help u with?

Explanation:

What is the function of the digestive system?

A. Removal of liquid waste

B. Maintain body temperature and hormone levels

C. Delivers food to your brain

D. Changes food into minerals and nutrients that are absorbed into
bloodstream

Answers

D. Changes food into minerals and nutrients that are absorbed into bloodstream

There are more than 300,000 species of plants that have been identified thus far in the world today; many new species are being discovered each year. In addition to providing most of the oxygen for land animals, plants do many other things for us. you will research the Internet and use reliable and credible scientific websites to investigate a specific plant species and choose a way (other than providing oxygen) that plants are useful to humans. [NOTE: Hot topic plants like Marijuana and Opium are off limits and cannot be discussed.]
Writing a full paragraph (a 150-words minimum) for your initial blog post discussing a specific species of plant and the benefit you chose. Be sure to describe what type of plant it is. Where is it found? How does it impact humans or other species? What role does it play in the ecosystem, etc.?

Answers

Answer and Explanation:

We can mention the advantages of the plants of the Fabaceae family, also known as legumes, such as beans, soybeans, peanuts and peas. First, we must mention how these plants have high nutritional values ​​providing high levels of iron and nitrogen that are essential elements for human health. However, a benefit that these planats produce and that is important on a worldwide scale is the fixation of nitrogen in the soil. All living beings need nitrogen in their bodies, since nitrogen is a primary element in the composition of nucleic acids and proteins. Animals are able to ingest nitrogen from the vegetable diet, but vegetables cannot absorb nitrogen from the atmosphere or nitrogen from the soil, because they are not in molecular forms that plants can absorb. To reverse this, farmers put chemical fertilizers on the plantations, but these fertilizers pollute the environment intensely. It is in this scenario that the plants of the Fabaceae family are important. This is because these plants are able to associate with bacteria that can capture atmospheric nitrogen and place it in the soil, in the molecular form that all plants can absorb. This allows plants to have the necessary nutrients for their growth, reduces environmental pollution, decreases spending on agricultural production, promotes correct levels of nitrogen for human consumption and allows agricultural products to be healthier.

The table below shows the average distances of four planets from the sun.

Considering Kepler's Laws, which planet has the fastest average orbital speed?

A. Venus
B. Neptune
C. Saturn
D. Jupiter

Answers

Answer:

Venus

Explanation:

The closer a planet is to the Sun the faster it moves because the Sun can exert more gravitational force on it. Venus is the second closest planet to the Sun therefore the fastest out of the options.

Its Venus.

It is closer to the sun than the other planets on here.

Mutations are a source of variation in:
A)asexually reproducing organisms
B)neither option
C)both options
D)sexually reproducing organisms

Answers

C( both options. Hope it helps!!!!

Which of the following symptoms is absent in “the depletion syndrome of the elderly”?
feeling worthless
losing interest in things
physical symptoms
self-blame

Answers

Answer:

self-blame i think if i am wrong pls tell me

If making a protein was like making a building, then the DNA molecule is like the blueprints and the RNA molecules are like the construction workers
True
False

Answers

I’m pretty sure it’s true. It makes the most sense.

How does genetic variability allow these lizard populations to survive ?

Answers

It allows the individual in a populations to survive and have offspring with the desire trait needed to live while those without will die out

Oxygen molecules are sixteen times as massive as hydrogen molecules.

At the same temperature, which type of molecule would you expect to move faster?

Answers

Answer: hydrogen molecules.

Explanation: in same temperature molecules have same average kinetic energy.

You can estimate it solving E = 3/2 kT = 1/2 mv². Constant k is Boltzmann constant.

Lighter molecule has greater speed

NEED ANSWER ASAP (95 points)

1.Give an example of one type of mutation, Give a Definition of it, and explain what is might do.


2.Give an example of how the environment can effect an organsism's genetics?


3.If a mutation happens in a somatic cell, will it be passed on to the next generation? Why or why not?


4.Explain the difference between a GENE and an ALLELE


5.What is the advantage of CROSSING OVER in Meiosis?


6.If the FREQUENCY of a trait is INCREASING in a population of animals......what might that mean?



7.Explain very briefly what causes Trisomy 21


8.Imagine a population of animals is separated by a natural event....and half of them a forced into a valley, and the other half are forced into the mountains. Imagine both populations survive. What type of changes might you notice a million years later between the 2 populations? Why is this?


9.What are 3 things you know about Genetic Variation now that you didn't know before this Unit?

Answers

1. Mutation in the human cells. When our bodies are cold the coldness responds back to our body by bringing air into it.

1. Mutation in the human cells. When our bodies are cold the coldness responds back to our body by bringing air into it.2 It can either increase me lan in or decrease it.

1. Mutation in the human cells. When our bodies are cold the coldness responds back to our body by bringing air into it.2 It can either increase me lan in or decrease it.3. Yes every generation is different.

1. Mutation in the human cells. When our bodies are cold the coldness responds back to our body by bringing air into it.2 It can either increase me lan in or decrease it.3. Yes every generation is different.4.An allee is A cell in our bodies which function blood cells. A gene is A trait from either a relative.

1. Mutation in the human cells. When our bodies are cold the coldness responds back to our body by bringing air into it.2 It can either increase me lan in or decrease it.3. Yes every generation is different.4.An allee is A cell in our bodies which function blood cells. A gene is A trait from either a relative.5. Meiosis Gives us more oxygen to breath in our Booties ☺ and our main body Functions.

1. Mutation in the human cells. When our bodies are cold the coldness responds back to our body by bringing air into it.2 It can either increase me lan in or decrease it.3. Yes every generation is different.4.An allee is A cell in our bodies which function blood cells. A gene is A trait from either a relative.5. Meiosis Gives us more oxygen to breath in our Booties ☺ and our main body Functions.6. Then that trait would most likely be passed on to the offspring/ Children.

1. Mutation in the human cells. When our bodies are cold the coldness responds back to our body by bringing air into it.2 It can either increase me lan in or decrease it.3. Yes every generation is different.4.An allee is A cell in our bodies which function blood cells. A gene is A trait from either a relative.5. Meiosis Gives us more oxygen to breath in our Booties ☺ and our main body Functions.6. Then that trait would most likely be passed on to the offspring/ Children.7. the most common form of Down syndrome, caused by an extra copy of chromosome number 21.

1. Mutation in the human cells. When our bodies are cold the coldness responds back to our body by bringing air into it.2 It can either increase me lan in or decrease it.3. Yes every generation is different.4.An allee is A cell in our bodies which function blood cells. A gene is A trait from either a relative.5. Meiosis Gives us more oxygen to breath in our Booties ☺ and our main body Functions.6. Then that trait would most likely be passed on to the offspring/ Children.7. the most common form of Down syndrome, caused by an extra copy of chromosome number 21.8. Both will collapse and collide together.

1. Mutation in the human cells. When our bodies are cold the coldness responds back to our body by bringing air into it.2 It can either increase me lan in or decrease it.3. Yes every generation is different.4.An allee is A cell in our bodies which function blood cells. A gene is A trait from either a relative.5. Meiosis Gives us more oxygen to breath in our Booties ☺ and our main body Functions.6. Then that trait would most likely be passed on to the offspring/ Children.7. the most common form of Down syndrome, caused by an extra copy of chromosome number 21.8. Both will collapse and collide together.9. Genetic Variation The process of a Trait getting passed down in various ways.

Explanation:

God Bless You!☺

Which organism described below makes its own food?
yoo yoo ill rlly apriciate if someone helps me

Answers

A
Explanation:
everything else finds or eats food that was made otherwise.


Need help with this!! I’m not good at writing these.

Answers

Answer:

The difference between ethical, and finally scientific questions, are based upon their root word. Such as an ethical question may involve race and heritage, while a legal question may involve anything that involves the law such as court questions and can be resolved by the judge not the jury. Finally scientific questions can involve a question thats mainly based around science such as items that you can test or measure.

4.

Humans contain 23 pairs of chromosomes in their body cells. After mitosis, it is expected that the daughter cells would each have a total of chromosomes _____. In sex cells undergoing meiosis I, it is expected that the two daughter cells would contain _____ chromosomes,and after meiosis II, ______ chromosomes.


A. 23, 23, 23

B. 46, 12, 12

C. 46, 23, 23

D. 46, 46, 46​

Answers

Answer:

B.46, 12, 12

Explanation:

#CARRY LEARNING

THANKS ME LATER

What are there scientific name.

Please Answer​

Answers

Answer:

manubruim = trapezoid

I hope it helps you

What are the effects of cystic fibrosis

Answers

Answer: the effects of cystic fibrosis are thick mucus that clogs certain organs, such as the lungs and intestines etc . This may cause malnutrition, poor growth, frequent respiratory infections, breathing problems, and chronic lung disease.

As a discussed in the lesson, eukaryotic cels are differ from prokaryotic cells based on what three things?

Answers

Answer:

Like a prokaryotic cell, a eukaryotic cell has a plasma membrane, cytoplasm, and ribosomes, but a eukaryotic cell is typically larger than a prokaryotic cell, has a true nucleus (meaning its DNA is surrounded by a membrane), and has other membrane-bound organelles that allow for compartmentalization of functions

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