During genetic engineering, how do Restriction enzymes know what base pairs to act on? A. Ligase bonds it to the correct spot. B. They don’t, the scientist must target it manually. C. They bond to the Recognition site. D. It recognizes the sticky ends.

Answers

Answer 1

Answer:

C

Explanation:

Restriction enzymes, also known as restriction endonucleases or DNA-cutter identify the base pairs to act on by targeting and binding to the recognition site in a gene.

The recognition site in a gene/DNA consists of four to eight palindromic nucleotide sequence. It is a region that the restriction enzyme cuts during the genetic engineering process in order to produce sticky or blunt ends. A gene might contain more than one recognition site.

Restriction enzymes are usually found in prokaryotic organisms, especially bacteria where they function primarily as defense molecules against bacteriophages.

Correct option: C


Related Questions

A research team discovered that a novel hormone X stimulates an enzyme that hydrolyzes proteins in the small intestine. However, when hormone X was added to a test-tube mixture containing the enzyme and proteins, no breakdown occurred.

Required:
a. Why is this the case?
b. Name ONE signal transduction pathway that hormone X is using to transfuse its signal. (write all the steps and intermediates)

Answers

Answer:

a. It is a hormone that acts upstream in the signaling pathway (i.e., acting as a ligand by binding to cell receptors)

b. Dopamine-mediated pathways  

Explanation:

It is well known that many cellular transduction pathways are activated via binding of signaling molecules (ligands) to receptors capable of triggering signaling cascades into the cell. For example, dopamine is a signaling hormone (neurotransmitter) involved in diverse brain processes (i.e., motor control, emotional response, etc). It is a neurotransmitter that binds to dopamine receptors and then stimulates the enzyme adenylate cyclase in a process that subsequently activates the production of the second messenger cyclic adenosine monophosphate (cAMP). Finally, cAMP acts as an intracellular signal transductor that play roles in many biological processes by transferring into the cell the effects of dopamine.

Each of two parents has the genotype brown/red​, which consists of the pair of alleles that determine hair color​, and each parent contributes one of those alleles to a child. Assume that if the child has at least one brown ​allele, that color will dominate and the​ child's hair color will be brown. a. List the different possible outcomes. Assume that these outcomes are equally likely. b. What is the probability that a child of these parents will have the red/red ​genotype? c. What is the probability that the child will have brown hair color​? a. List the possible outcomes.

Answers

Answer:

a. In image attached.

b. 25% or a 1 in 4 chance.

c. 75% or a 3 in 4 chance.

Explanation:

B = Dominant (brown gene)

r = Recessive (red gene)

If two parents both have Br genes, they will both have brown hair.

The Punnet square will look like the image attached.

Pectoralis Minor External Obliques Gluteus maximus Hamstrings Which are axial muscles or appendicular muscles?

Answers

Answer:

they are axial muscles

Which of the following properties is the temperature at which a liquid turns to gas? (3 points)
оа
Magnetism
Ob
Thermal conductivity
ос
Melting point
Boiling point
Od

Answers

thermal conductivity

Answer:

Boiling point

Explanation:

I did the test

What are the
traditional
circulatory
endpoints of
resuscitation
in shock?​

Answers

Explanation:

traditional circulatory endpoints of resuscitation in shock include hypertension,tachycardia,decreased mentation and urine output

When an enzymatic reaction is in progress, do you expect to see an increase, decrease or no change in each of following: (i) substrate (ii) product (iii) enzyme

Answers

Answer:

During Enzymatic reaction, enzymes binds to a substrate to form a which then forms the product.

Substrate: A reactant in an enzymatic or chemical reaction is called substrate. Product: The final outcome or substance after completion of reaction is called product.Enzyme: Enzyme helps to increase the rate of reaction and act as a catalyst.

Change in shape of substrate, product and enzymes depends on the temperature outside the optimal range which affect bonding between enzyme and substrate. Enzymes definitely changes their size when bind to substrat and the process is called induced fit which increases the rate of reaction.

During an enzymatic reaction, their is an decrease in the concentration of substrate, an increase in the concentration of product, and no change in the concentration of the enzyme.

What is enzymatic reaction?

A reaction, which is mediated with the help of an enzyme is termed as enzymatic reaction. A substance, which functions as a catalyst in living organisms and regulate the rate at which the chemical reactions takes place is known as an enzyme.

The enzymatic reactions follows the lock and key model, in which the free enzyme combines with the substrate and forms the enzyme-substrate complex, this is further catalyzed into the enzyme-product complex, from which the free enzyme and product get discharged. The product never further binds with the free enzyme.

In the reaction, the substrate get used up thus its concentration decreases, while the formation of product takes place thus its concentration increases. However, there is no change in the concentration of the enzyme as it does not get used up in the process.

Thus, in enzymatic reaction, substrate decreases, product increases, and enzyme concentration remains the same.

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What is the maximum magnification of most classroom compound light microscopes? 500x 1,000x 2,500x 5,000x

Answers

Answer:

2,500

Explanation:

Answer:

the answer is 2,500x

Match the reactant in gluconeogenesis with the appropriate enzyme.

Enzyme:

a. Fructose dehydrogenase
b. PEP kinase
c. Phosphoglycerase
d. Pyruvate kinase
e. Phosphoglyceromutase
f. Glucose kinase
g. Phosphoglycerokinase
h. Phosphofructokinase
i. Hexokinase


1. glucose
2. fructose-6-phosphate
3. fructose-I , 6-bisphosphate
4. phosphoenolpyruvate
5. 1 , 3-bisphosphoglycerate
6. 3-phosphoglycerate

Answers

Answer: a c g e

Explanation:

The main evolutionary consequence of the process of 'plate tectonics' has been

Answers

The movement of plate tectonics produces new environments and gives organisms a new environment that they now need to adapt and evolve to fit into.

In the image, the arrow Is pointing to a celestial object. Which attribute disqualifies the object from being a planet?
A. It appears to have no moons.
B. It is spherical in shape.
C. Its neighborhood is not clear.
D. It has horizontal bands on its surface.

Answers

The correct answer is C. Its neighborhood is not clear.

Explanation:

One of the key features of a planet is that it clears its neighborhood; this means the planet gravitational force is strong enough that there are no other celestial bodies on its orbit or next to it except by its moons or satellites. This does not occur in the image presented because there are smaller celestial bodies near to the main or bigger celestial body. This likely occurs if the celestial body is not big enough to clear its neighborhood, and therefore it is not a planet.

21. When looking at the effect of pH on sucrase activity, what was the wavelength of light
used in the spectrophotometer?​

Answers

Answer:

540nm

Explanation:

Sucrase is one of the digestive enzymes responsible for the digestion of sucrose containing foods. Its major function is the catalysis of the hydrolysis of sucrose into its subunits; fructose and glucose.

Enzymes, as biochemical catalysts always function maximally at an optimum pH. As the pH of the system departs from the ideal pH for any enzyme, the activity of that enzyme decreases accordingly.

The activity of sucrose is studied using a spectrophotometer of wavelength about 540nm. As the pH of the solution departs from the specific ideal pH of sucrase, its activity decreases as stated above.

Is the talc renewable?

Answers

Answer:

no.its nonrenewable.

talc is nonrenewable.

thanks.

3'T.A.C.C.A.T.A.T.T.G.A.G.5'

Answers

Answer:

a u g g u a u a a c u c

Explanation:

i think this is it, but there is no explanation of what to do

Which type of nuclear power reactor is used in only 1 percent of all power plants?

Answers

Answer:

Breeder reactors

Explanation:

The type of nuclear power reactor that is used in only 1 percent of all power plants would be Breeder reactors.

1. Research 5 functions of the frontal lobe and explain them in detail with example.
2. Explain what you think might happen if someone damaged frontal lobe. You must mention the functions you have listed

Answers

Answer:

Solving a problem, memory, language, judgement,  social and sexual behavior.

Explanation:

5 functions of the frontal lobe are given below:

1) it control the expression of emotions.

2) it is responsible in solving a problem.

3) it also control the memory and language.

4) it helps in judgement.

5) it is responsible for social and sexual behavior.

If frontal lobe is damaged, the individual may lose its memory, judgement ability. the social and sexual behavior is also disturbed. It may lose the ability to solve a problem and also have language problem.

Your town is considering building a bridge across a large lake. Much of the town's drinking water comes from this lake. Before
they make a decision, they hire a team of scientists to investigate the impact of building this bridge. Which statement best
describes why this study is important?
The project will stimulate the town's economy by providing many employment opportunities for the town's residents.

There will be many people opposed to the bridge project, and the study will provide results that will persuade them to
change their minds.

The study will provide the potential risks and benefits of the project and help the town make an informed decision.

If there is a problem after the bridge is built, the town can use the study to impose a fine against the scientists who
conducted the study.

Answers

Answer:

C. The study will provide the potential risks and benefits of the project and help the town make an informed decision.

Explanation:

Answer:

c is the anwser

Explanation:

I took the test

DNA is transcribed to messenger RNA (mRNA), and the mRNA is translated to proteins on the ribosomes. A sequence of three nucleotides on an mRNA molecule is called a codon. As you can see in the table, most codons specify a particular amino acid to be added to the growing protein chain. In addition, one codon (shown in blue) codes for the amino acid methionine and functions as a "start" signal. Three codons (shown in red) do not code for amino acids, but instead function as "stop" signals.
Sort the following ten codons into one of the three bins, according to whether they code for a start codon, an in-sequence amino acid, or a stop codon
AUG, UAG, UAA, UGA, AAA, UGC, AUC, CAC, GCA, ACU

Answers

Answer:

start codon - AUG

in-sequence amino acid - AAA, UGC, AUC, CAC, GCA, ACU

stop codon - UAA, UAG, UGA

Explanation:

From the amino acid chart, the start codon is represented by the codon AUG which also code for the amino acid methionine. There are three stop codons including UAA, UAG, and UGA. The stop codons signal the end of the translation process of the mRNA. Every other codon within the mRNA chain codes for different amino acids except methionine.

Hence, using the attached amino acid chart:

start codon - AUG

in-sequence amino acid - AAA, UGC, AUC, CAC, GCA, ACU

stop codon - UAA, UAG, UGA

Every interaction with the outside world is because of A) epithelial tissue B) connective tissue C) nervous tissue D) muscular tissue

Answers

Answer:

A cutaneous membrane is a multi-layered membrane composed of epithelial and connective tissues. The apical surface of this membrane exposed to the external environment and is covered with dead, keratinized cells that help protect the body from desiccation and pathogens....

Is this statement true or false?

A hypothesis is a lucky guess.

Answers

True because it can either be wrong or right

Answer:

false!

Explanation: i took the test

Which of the following is not a function of Mitosis? A. Growth and development B. Produce genetically identical daughter cells C. Produce genetically different daughter cells D. Replace damaged cells

Answers

C. Produce genetically different daughter cells, is not a function of mitosis

Which area of the respiratory system is vascularized with the richest capillary network in the body?​

Answers

Answer:

There are from 200-500 million alveoli (mean diameter = 200 micrometers) in adult human lungs

Explanation:

The epithelial cells of the alveolar septum are markedly thinned and the capillary network immediately beneath the epithelium is the richest in the body.hope this helps you :)

Answer:

The answer is alveoli

10. Write the two likely errors carried out while using ruler during measuring length of anywhy did to like me process carried out while using a ruler during measuring length of any object

Answers

Answer:

positive error and negative error.

Explanation:

There are two types of error which is possible when measuring of a length with the help of ruler. Positive error occurs if the calculated value is larger than actual value while negative error occurs when the calculated value is smaller than actual value. To avoid positive error, the increased value should be subtracted from calculated value while negative error can be corrected by adding the value with the reading.

whats the answer guys help me out

Answers

Answer:

Read Exp:

Explanation:

1st one - Attracts Pollinators.

2nd one -  Prevents water loss.

3rd one - Traps insects.

what are 3 major functions of the femur?

Answers

Answer:

The femur is the longest bone in the human skeleton. It functions in supporting the weight of the body and allowing motion of the leg. The femur articulates proximally with the acetabulum of the pelvis forming the hip joint, and distally with the tibia and patella to form the knee joint.

Explanation:

Holding the body weight once standing and moving. People are being stabilized as they move. Connecting the hips and knees' muscles, tendons, and ligaments to the rest of your body. These are three functions of femur.

What is femur?

The femur is the bone in the thigh. It is person's body's longest and strongest bone. It is an essential component of the ability to stand and move.

There can be many functions of this bone, some are listed below:

Hold the body weight.Stabilize the body while moving.Connecting hip and knees.

Thus, above mentioned are three functions of femur.

For more details regarding femur, visit:

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what is the first step you would take to solve this equation and why 49n+986=-43​

Answers

Answer:

Subtracting 986 from both sides.

Explanation:

Your goal is to isolate n.

Which two layers are approximately the same age?
core sample 1
core sample 2

Answers

Complete question:

Examine the following diagrams. Columns I and II contain rock layers A, B, C, D, E, and F. Both columns were taken from the same dig site at different locations. Which two layers are of approximately the same age? (Note: The diagrams are attached).

Answer:

C and D  

Explanation:  

Layers C and D apparently contain the same fossilized organisms in the same substrate. Their appearance is very similar. By knowing that certain organisms used to live in a certain period of time, if we find two strata that show the same fossilized organisms, then we can estimate that these two strata are the same age. Fossilized organisms are used to estimate the age of a rocky stratum.

Life history can be illustrated through fossilized organisms trapped or converted into rocks. These fossils are known as index or guide fossils, of animal or vegetal nature, and they can apport information about the rocky stratum in which they are located or incrusted. These fossils are used to determine the age of the rocky sediments or the environmental conditions under which they were deposited.

 

Answer:both of them are

Explanation:

If the gene encoding one of the proteins that forms cohesin were mutated such that cohesin could not perform its normal function, what would be the consequence? Check all that apply.

Answers

Answer:

The following apply:

- Chromosomes would not be able to condense.

- Chromosomes would not be able to properly align at the metaphase plate.

- The separation of sister chromatids would not likely occur specifically during anaphase A.

- The nuclear envelope would not be able to reform.

Have you ever noticed that aside from zoos, you don't see kangaroos, wombats, and koala bears outside of Australia? This is because there is a trench that is a "biogeographical transition zone between Asian and Australian flora and fauna". What is the name of that trench?

Answers

Answer:

Wallace Line

Explanation:

Wallace Line is a boundary line which is a transitional zone between Asia and Australia. This boundary drawn by the British naturalist Alfred Russel Wallace in 1859. This boundary restricts the transferring organisms such as flora and fauna from Australia to Asia. So that's why kangaroos, wombats, and koala bears are only present in Australia not in the continent of Asia.

What growth information leads to your answer? The colonies are able to grow on minimal medium containing no additional supplements. The colonies are able to grow on complete medium.

Answers

Answer:

A growth medium or culture medium is a solid, liquid or semi-solid designed to support the growth of a population of microorganisms or cells via the process of cell proliferation,[1] or small plants like the moss Physcomitrella patens.[2] Different types of media are used for growing different types of cells.[3]

The two major types of growth media are those used for cell culture, which use specific cell types derived from plants or animals, and microbiological culture, which are used for growing microorganisms, such as bacteria or fungi. The most common growth media for microorganisms are nutrient broths and agar plates; specialized media are sometimes required for microorganism and cell culture growth.[1] Some organisms, termed fastidious organisms, require specialized environments due to complex nutritional requirements. Viruses, for example, are obligate intracellular parasites and require a growth medium containing living cells.

What is the maximum magnification of most classroom compound light microscopes? 500x 1,000x 2,500x 5,000x

Answers

Answer:

2,500x

Explanation:

am doing the review rn

Answer:

1,000x

Explanation:

i got yall

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