Fluorometers are designed so that the path of the excitation light is at a right angle to the path of the emitted light. What is the purpose of this design

Answers

Answer 1

Answer: prevent excitation light from reaching the detector

Explanation:

A fluorometer refers to the device that's used in the measurement of parameters that are of visible spectrum fluorescence. They also prevent excitation light from reaching the detector.

These parameters are used in the identification of the amount and presence of molecules in a medium.


Related Questions

How much energy does it take to boil water for pasta? For a one-pound box of pasta
you would need four quarts of water, which requires 15.8 kJ of energy for every degree Celsius (°C) of temperature increase. Your thermometer measures the starting
temperature as 48°F. Water boils at 212°F.


a. [1 pts] How many degrees Fahrenheit (°F) must you raise the temperature?
b. [2 pts] How many degrees Celsius (°C) must you raise the temperature?
c. [2 pts] How much energy is required to heat the four quarts of water from
48°F to 212°F (boiling)?

Answers

Answer:

a. 164°F

b. [tex]91.\overline 1 \ ^{\circ} C[/tex]

c. [tex]140.\overline 4[/tex] kJ

Explanation:

The starting temperature of the water, T₁ = 48F

The temperature at which the water boils, T₂ = 212°F

a. The difference between the initial and the boiling water temperature, ΔT = T₂ - T₁

Therefore;

ΔT = 212°F - 48°F = 164°F

The temperature by which he temperature must be raised, ΔT = 164°F

b. 48°F = ((48 - 32)×5/9)°C = (80/9)°C = [tex]8.\overline 8 \ ^{\circ} C[/tex]

212°F = ((212 - 32)×5/9)°C = 100°C

∴ ΔT = 100°C - [tex]8.\overline 8 \ ^{\circ} C[/tex] = [tex]9.\overline 1 \ ^{\circ} C[/tex]

c. The heat capacity of the water = The heat required to increase four quartz of water by 1 °C = 15.8 kJ

∴ The heat required to raise four quartz of water by [tex]9.\overline 1 \ ^{\circ} C[/tex], ΔQ = 15.8 kJ/°C × [tex]9.\overline 1 \ ^{\circ} C[/tex] = [tex]140.\overline 4[/tex] kJ.

(a) What is the escape speed on a spherical asteroid whose radius is 301 km and whose gravitational acceleration at the surface is 0.412 m/s2

Answers

Answer:

[tex]V.E=498.02m/s^2[/tex]

Explanation:

From the question we are told that:

Radius [tex]r=301Km[/tex]

Gravitational acceleration [tex]g=0.412 m/s^2[/tex]

Generally the equation for Escape velocity is mathematically given by

 [tex]V.E^2=2gr[/tex]

 [tex]V.E^2=2*0.412m/s^2*301000[/tex]

 [tex]V.E^2=248024[/tex]

 [tex]V.E=\sqrt{248024}[/tex]

 [tex]V.E=498.02m/s^2[/tex]

Is torque only produced when the force is applied perpendicular to the moment arm?

Answers

Yes. Only a perpendicular component produces a torque.

The Torque is only produced when the force is applied perpendicular to the moment arm.

What is moment of force?

The equal and opposite force acting at a point from the axis of rotation is called the moment.

M = F x r

Moment of force is also called as Torque.

Moment arm is the distance of the point of application of force from the axis of rotation of the body.

Thus, the perpendicular component only, produces the torque.

Learn more about moment of force.

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A dog wishes to swim across a slow moving stream. The current velocity is 3.0m/s. The distance directly across the stream is 50m. If the dog point himself directly across the stream, it will take .....

Answers

Answer:

Time = 16.667 seconds

Explanation:

Given the following data;

Distance = 50 meters

Velocity = 3 m/s

To find the time it will take;

Velocity can be defined as the rate of change in displacement (distance) with time. Velocity is a vector quantity and as such it has both magnitude and direction.

Mathematically, velocity is given by the equation;

Velocity = distance/time

Making time the subject of formula, we have;

Time = distance/velocity

Time = 50/3

Time = 16.667 seconds

Determine the amount of potential energy of a 4.2 kg book that is placed on a shelf with a height of 0.9 meters. Round your answer to one decimal place​

Answers

Answer:

that is a correct answer maybe .

A stationary body explodes into four identical fragments such that three of them fly off mutually perpendicular to each other, each with same KE, E 0 ​ . The energy of explosion will be Aa

Answers

Answer:

6Eo

Explanation:

The power of the kettle was 1.5 kW. The 0.2kg heating element took 5 seconds to heat from 20 °C to 100 °C. Calculate the specific heat capacity of water using this information.

Answers

Answer:

Specific heat capacity, c = 468.75 J/Kg°C

Explanation:

Given the following data;

Power = 1.5 kW to Watts = 1.5 * 1000 = 1500 Watts

Time = 5 seconds

Mass = 0.2 kg

Initial temperature = 20°C

Final temperature = 100°C

To find specific heat capacity;

First of all, we would have to determine the energy consumption of the kettle;

Energy = power * time

Energy = 1500 * 5

Energy = 7500 Joules

Next, we would calculate the specific heat capacity of water.

Heat capacity is given by the formula;

[tex] Q = mcdt[/tex]

Where;

Q represents the heat capacity or quantity of heat. m represents the mass of an object. c represents the specific heat capacity of water. dt represents the change in temperature.

dt = T2 - T1

dt = 100 - 20

dt = 80°C

Making c the subject of formula, we have;

[tex] c = \frac {Q}{mdt} [/tex]

Substituting into the equation, we have;

[tex] c = \frac {7500}{0.2*80} [/tex]

[tex] c = \frac {7500}{16} [/tex]

Specific heat capacity, c = 468.75 J/Kg°C

the electric charge remains at rest in a. static electricity, b. current electricity, c. none of these​

Answers

Answer:

Option (a) is correct.

Explanation:

The electric charge is the inherent property of matter which appears due to the deficiency of charge or the excess number of electrons.

There are two types of charges, positive and negative.

When an object has deficiency of charge it becomes positive charge and when it gains some electrons, it becomes negative charge.

When the charge is in motion, it is called electric current and when the charge is at rest, it is called static current.

A charge at rest contributes the electric field only while the charge is in motion contributes the electric and magnetic field both.

Mean diameter of cylinder measured by A is 2.50 cm with a possible error of 0.25 cm whereas mean height of cylinder measured by B is 10.0 cm with a possible error of 0.5 cm. Whose measurement is more accurate?

Answers

Answer:

The measurement of first cylinder is more accurate.(A)

Explanation:

The least possible error higher will be accuracy.

What is the relationship between Avogadro's number and a mole?
A. There are 6.02 x 1023 items in a mole, which equals Avogadro's
number
B. A mole is a smaller amount than Avogadro's number.
C. Avogadro's number is used to count particles, and a mole is used
to measure mass.
D. A mole applies to any item, but Avogadro's number is limited to
atoms.
SUBM

Answers

Answer:

A. There are 6.02 x 1023 items in a mole, which equals Avogadro's

number

Explanation:

The mole of a substance is

The U.S. has plans to build a 30-meter telescope, while the Europeans are thinking about a 39-meter telescope. What technological innovation allows astronomers to be thinking about telescopes with that large an aperture

Answers

Answer:

The mirror in these telescopes will be constructed from many smaller mirrors which will work together.

Explanation:

A telescope is defined as an optical instrument that is used to observe or view the distant objects. A telescope is made up of curved lenses or mirrors or by combining both.

In the context, both the US and the Europeans are planning to make a telescope which is 30 m and 39 m respectively. This could be possible as the mirrors that are used to make these telescope will be made from a many smaller mirrors that will work together as a single large mirror having a very large aperture.

Why would an airplane flying at 10,000 meters above the ground have more gravitational potential
energy than the same airplane flying at 1,000 meters above the ground?

Answers

Answer:

The gravitational potential energy (gpe) possessed by an object or body is directly proportional to the height of the object or body.

Explanation:

Gravitational potential energy (GPE) is an energy possessed by an object or body due to its position above the earth.

Mathematically, gravitational potential energy is given by the formula;

G.P.E = mgh

Where;

G.P.E represents potential energy measured in Joules.

m represents the mass of an object.

g represents acceleration due to gravity measured in meters per seconds square.

h represents the height measured in meters.

Generally, the gravitational potential energy (gpe) of an object or body is directly proportional to the height of the object or body. Thus, the gravitational potential energy of a body increases as the height of the body increases.

In conclusion, an object with a higher height would have a higher gravitational potential energy.

What is an electric fuse? What is the working principle of electric fuse?

Answers

What is an electric fuse?Answer: Electric fuse is a safety device used to limit the current in an electric circuit which melts and breaks the circuit whenever there is an excess flow of current through the circuit.What is the working principle of electric fuse?An electric fuse is based on the principle of heating effect of electric current. It is made up of thin metallic wire of non-combustible material. A fuse is always connected between the ends of the terminal in a series connection with the circuit.

Which two factors affect the size of the gravitational field?

Answers

Answer:

Explanation:

mass and distance

How does the construction of dams positively affect natural resources?
by providing water for irrigation and restoring trees to areas where forests once existed
by creating reservoirs, preventing flooding, and renewing destroyed ecosystems
by preventing flooding, creating reservoirs, and providing water for irrigation
by renewing destroyed ecosystems and restoring trees to areas where forest once existed

Answers

Other person is incorrect, I did the test on edg.

Answer:  

A. by providing water for irrigation and restoring trees to areas where forests once existed

The construction of dams positively affect natural resources by providing water for irrigation.

What is natural resources?

Natural resources serves as materials thst is found in nature which are used by humans. These natural substances are soil, water.

Therefore, building of dams can help irrigation by using water which is a natural resources.

Learn more about natural resources at;

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What is the current flowing through the circuit shown? (V= 110 V, R, = 200, R2 = 300, R3 = 10 0) (Ohm's law: V = IR)
A. 1.8 A
B. 20 A
C. 0.05 A
D. 0.55 A​

Answers

Answer:

A

Explanation:

The first thing you have to do is go back and list the resistances correctly. R1 = 20 R2 = 30 and R3 = 10.

Leave the units off if you can't make an omega.

The resistance of a series circuit (that's what this is) is r1 + r2 + r3 = 10 + 20 + 30 = 60 ohms

Now use ohms law.

R = 60 ohms

V = 110 volts.

I = V / R

I = 110/60

I = 1.833 to the nearest 1/10 = 1.8

In 'coin on card' experiment a smooth card is used. ​

Answers

Answer:

In coin card experiment smooth card is used so that the card can slide easily from glass

In three words I can sum up everything I’ve learned about life: it goes on. ― Robert Frost

What is the size of the force acting on a copper wire with a magnetic flux density of 3.6 x 10-2 T acting at
right angles to the wire of length 24 m and current of C:048 A? Give your answer to an appropriate
number of significant figures.

Answers

Answer:

F = 0.414 N

Explanation:

Given that,

Magnetic flux density,[tex]B=3.6\times 10^{-2}\ T[/tex]

The length of the wire, l = 24 m

Current, I = 0.48 A

We need to find the force acting on the wire. The formula for the force is given by:

[tex]F=ILB[/tex]

Put all the values,

[tex]F=0.48\times 24\times 3.6\times 10^{-2}\\\\F=0.414\ N[/tex]

So, the force acting on the copper wire is equal to 0.414 N.

The magnetic force of the copper wire is  41.472 N.

Magnetic force of the copper wire

The magnetic force of the copper wire is calculated by applying the following equation.

F = BIL x sinθ

Where;

θ is the inclination of the magnetic fieldI is the currentL is the length of the wireB is the magnetic field strength = flux density

F = (3.6 x 10⁻²) x (48) x 24 x sin(90)

F = 41.472 N

Thus, the magnetic force of the copper wire is  41.472 N.

Learn more about magnetic force here:  https://brainly.com/question/13277365

help help help please please​

Answers

Answer:

below

Explanation:

that is the procedure above

Ehy Newton's law of gravitation also called universal law?

Answers

Answer:

Gravity is a natural phenomenon that causes all massive objects to attract each other. The magnitude or force of this interaction depends on the masses and distance of the bodies. This causes objects to fall toward Earth, for example, and keep satellites and celestial bodies in their orbits, such as the Earth's orbit around the Sun. Gravity also causes the tidal phenomenon.

In physics, gravity can be approximated by Isaac Newton's laws of gravity. Although the accuracy of the law is sufficient, for example, when calculating the orbits of spacecraft, gravity is best described by Albert Einstein’s general theory of relativity, in which it is considered the curvature of space-time.

As a girl pushes and object across a wood floor she suddenly comes to an area where the floor has been waxed recently making it slippery . What becomes true if the coefficient of kinetic friction

Answers

Answer:

This question is incomplete but the completed question is below

As a girl pushes an object across a wood floor, she suddenly comes to an area where the floor has been waxed recently, making it slippery. What becomes true of the coefficient of kinetic friction? A. The coefficient of kinetic friction increases. B. The coefficient of kinetic friction decreases. C. The coefficient of kinetic friction becomes zero. D. The coefficient of kinetic friction becomes negative.

The correct option is B

Explanation:

Coefficient of kinetic friction can be defined as the frictional force resisted by the motion of an object. From the question, it might take the girl to apply a force equivalent to just half that of the weight of the object to overcome friction to keep the object moving on a wood floor. Once she gets to the waxed area, the frictional force reduces, thereby also reducing the coefficient of kinetic friction further. Thus, she will be able to use less than half of the force (equivalent to less than half of the weight of the object) to push the object.

Thus, the correct option is B.

A squirrel jumps into the air with a velocity of 4 m/s at an angel of 50 degrees. What is the maximum height reached by the squirrel?

Answers

Answer:

Explanation:

Assuming the squirrel is jumping off the ground, here's what we know but don't really know...

v₀ = 4.0 at 50.0°

So that's not really the velocity we are looking for. We are dealing with a max height problem, which is a y-dimension thing. Therefore, we need the squirrel's upward velocity, which is NOT 4.0 m/s. We find it in the following way:

[tex]v_{0y}=4.0sin(50.0)[/tex] which gives us that the upward velocity is

v₀ = 3.1 m/s

Moving on here's what we also know:

a = -9.8 m/s/s and

v = 0

Remember that at the very top of the parabolic path, the final velocity is 0. In order to find the max height of the squirrel, we need to know how long it took him to get there. We are using 2 of our 3 one-dimensional equations in this problem. To find time:

v = v₀ + at and filling in:

0 = 3.1 - 9.8t and

-3.1 = -9.8t so

t = .32 seconds.

Now that we know how long it took him to get to the max height, we use that in our next one-dimensional equation:

Δx = [tex]v_0t+\frac{1}{2}at^2[/tex] and filling in:

Δx = [tex]3.1(.32)+\frac{1}{2}(-9.8)(.32)^2[/tex] and using the rules for adding and subtracting sig fig's correctly, we can begin to simplify this:

Δx = .99 - .50 so

Δx = .49 meters

Can somebody help me understand this

Answers

I think it’s C. Marshmallow and toothpicks are used to show the composition of a water molecule

(c) The metal block had a mass of 1.50 kg
The specific heat capacity of the metal was 900 J/kg °C
Calculate the change in thermal energy of the metal during 240 seconds.
Use the Physics Equations Sheet.
Give your answer in kilojoules.​

Answers

Answer:56

Explanation: I did the test

The decrease in the intensity of light over distance, such as with increasing depth in water, is known as ______.

Answers

Answer:

Attenuation

Step By step Explanation:

The decrease in the intensity of light over distance, such as with increasing depth in water, is known as ______.

It is called attenuation.

The decraese in the amplitude of the signals is called attenuation.

The weight of a body is 600 N. What is the mass of the body on the surface of the earth?​

Answers

Explanation:

soln,

weight=600N

mass=?

gravity=9.8 m/s²

now,

mass=weight/gravitymass=600/9.8mass=61.22kg

hope it helps.

stay safe healthy and happy.

Answer:

m = 61.22 kg

Explanation:

F = 600 N

g = 9.8 m/s²

m = ?

F = mg

600 = m(9.8)

---> m = 61.22 kg

d. 'g' vanishes at centre of
6) A prism of 6° is made of material of refractive index What is the
deviation caused by it
d. 40
c.120
a. 60
b.8°
7) An object is placed 1cm from lens having magnification 5. Then its foc
length is
a. 0.8 cm b. 1.2 cm c. -1.2 cm d. -0.8cm​

Answers

Answer:

a. 0.8 cm

Explanation:

The distance of the object from the lens, u = 1 cm

The magnification of the lens, m = 5

The focus of a lens formula is given as follows;

[tex]f = \dfrac{1}{\dfrac{1}{v} + \dfrac{1}{v} }[/tex]

The magnification of the lens, m = -v/u

Where;

v = The distance of the image from the lens

Therefore, we have;

v = m × u

∴ v = 5 × 1 cm = 5 cm (on the other side of the lens)

From which we get;

[tex]f = \dfrac{1}{\dfrac{1}{1} + \dfrac{1}{5} } = \dfrac{5}{6} \approx 0.8[/tex]

The focal length ≈ 0.8 cm

A car is stationary. It accelerates at 0.8 ms^2
for 10 s and then at 0.4 ms^2
for a further 10 s. Use
the equations of motion to deduce the car’s final displacement. You will have to split the journey
into two parts, since the acceleration changes after 10 s.

Answers

Answer:

the car’s final displacement is 60 m

Explanation:

Given;

initail velocity of the car, u = 0

acceleration of the car, a = 0.8 m/s²

time of motion, t = 10 s

The first displacement of the car:

[tex]x_1 = ut + \frac{1}{2} at^2\\\\x_1 = 0 + \frac{1}{2} (0.8)(10)^2\\\\x_1 = 40 \ m[/tex]

The second displacement of the car;

acceleration, a = 0.4 m/s²,   time of motion, t = 10 s

[tex]x_2 = ut + \frac{1}{2} at^2\\\\x_2 = 0 + \frac{1}{2} (0.4)(10)^2\\\\x_2 = 20 \ m[/tex]

The final displacement of the car;

x = x₁  +  x₂

x = 40 m  +  20 m

x = 60 m

Therefore, the car’s final displacement is 60 m

prove that vector addition obeys commutative and associative laws​

Answers

the order of vector addition doesn't affect the resultant vector and grouping or order of pair doesn't effect the sum.

A stone is thrown with an initial speed of 12 m/s at an angle of 30o above the horizontal from the top edge of a cliff. If it takes the stone 5.6 s to reach the bottom, how far does the stone travel horizontally

Answers

Answer:

[tex]d=58m[/tex]

Explanation:

From the question we are told that:

Initial Speed [tex]U=12m/s[/tex]

Time [tex]T=5.6s[/tex]

Angle [tex]\theta=30[/tex]

Generally the  Newton's equation for motion is mathematically given by

[tex]d=d'+ut+\frac{at^2}{2}[/tex]

[tex]d=12cos30*5.6[/tex]

[tex]d=58m[/tex]

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