How will you isolate DNA from a pure fungal culture

Answers

Answer 1

Answer:

Explanation:

There are several ways of isolating DNA from a pure fungal culture but you can use the CTAB method.

In this method, genomic DNA can be extracted.

First of all, the cell walls of fungal mycelia can first be broken down by grinding it using glass rods or you can use or liquid nitrogen. Place it in a plate. You can now CTAB extraction buffer , then incubate at 65°C, then let it undergo purification with phenol:chloroform:isoamyl alcohol 25: 24: 1 and carry out precipitation with isopropanol . Then the DNA can be extracted and then dissolved in 50 μl of pure water.


Related Questions

A membrane consisting only of phospholipids undergoes a sharp transition from the crystalline form to the fluid form as it is heated. What would be the effect on this transition in a membrane containing 80% phospholipid and 20% cholesterol

Answers

Answer:

Having 20% cholesterol in a membrane would reduce the fluidity.

Explanation:

At high temperatures, cholesterol stabilizes the membrane and increases the melting point.

The plasma membrane is the layer that surrounds the cell and is composed of cholesterol and phospholipid. The presence of cholesterol reduces the fluidity and transition.

What are the components of the plasma membrane?

The plasma membrane separates the exterior and the interior of the cell and through a semi-permeable nature allows the transport of the molecules. It is composed of phospholipids and cholesterol molecules.

The phase transition of the membrane is known by the change in the rigidity as it starts free-flowing at a high temperature called transition temperature.

In the presence of cholesterol, the movement and the fluidity reduce as it interferes with the fatty acid chain and now requires more temperature to convert the rigid structure to become fluid.

Therefore, membrane-containing cholesterol requires more temperature to modify the fluidity as compared to phospholipids alone.

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Elephants are considered K-strategists because they give birth to only one calf at a time and most have five years in between having another calf. Calves stay under the protection of the mother and her herd until maturity at 10-15 years of age. What type of survivorship curve do elephants tend to follow

Answers

Answer:

Type I curve

Explanation:

The K-strategist are species characterized by relatively stable populations that fluctuate at the carrying capacity of the habitat or niche in which organisms reside. Elephants are considered as K-strategists because they have a low population growth rate and relatively stable populations. There are three different types of survivorship curves. The Type I curve (also referred to as A curve) is characteristic of k-strategist organisms. Humans and elephants exhibit a Type I survivorship curve in which organisms tend to die when they become elderly. These species have a small number of offspring and provide parental care to ensure their survival. In a Type II survivorship curve, species produce many offspring and only some offspring survive (e.g., birds), while in Type III survivorship curve organisms produce many more offspring and most do not survive (i.e., r-strategists such as frogs or insects).

The passage suggests that in designing the experiment, Johnson relied on the fact that A) fungal hyphae warn beanstalks of danger if a nearby plant is attacked. B) wasps are harmful to the ongoing existence of broad bean plants. C) broad bean plants release noxious chemicals to ward off infestation. D) aphids are able to withstand the aggressive maneuvers used by wasps.

Answers

the correct answer is AlC

characteristics of Pisces

Answers

Answer:

Pisces are known as amazingly imaginative, creative, empathetic, and kind, they can also be overly sentimental, impressionable, and closed off.

Explanation:

Hope this helps!

In pea plants, flowers are either white or purple: the purple color is produced by pigments called anthocyanins. The production of anthocyanins is a two-step process: the first step is controlled by the C gene and the second by the P gene. Both genes must produce functional proteins for anthocyanin to be produced. This is an example of:

Answers

Answer:

This is an example of gene translation.

Explanation:

Gene translation refers to the genetic process where a set of genes is used to create amino acids that will be responsible for creating a protein needed to perform some function or characteristic of the organism. In the question above, we can see that anthocyanin, responsible for the pigment of flowers, is created through the work of proteins that are created from gebes C and P. This is an example of gene translation, as it presents the formation of proteins regulated by genes.

oxygen is carried by blood to all the cells of body in the form of ___________​

Answers

Answer:

Oxygen is carried by blood to all the cells of the body in the form of haemoglobin.

Explanation:

Oxygen is carried in the blood in two forms:

(1) dissolved in plasma and red blood cells water (about 2% of the total)

(2) reversibly bound to haemoglobin (about 98% of the total).

Haemoglobin is a protein in the red blood cells and carries oxygen around our bodies. At the same time, carbon dioxide that is dissolved in the blood comes out of the capillaries back into the air sacs, ready to be breathed out.

Describe how and where viruses reproduce and the function of RNA and DNA in this process.

Answers

Answer:

Using the host's cellular metabolism, the viral DNA begins to replicate and form proteins. Then fully formed viruses assemble. These viruses break, or lyse, the cell and spread to other cells to continue the cycle. Like the lytic cycle, in the lysogenic cycle the virus attaches to the host cell and injects its DNA.

explain what it means to view something from a frae of reference. provide an example that illustrate your explanation.

Answers

Answer:

ex. where you are standing now

a perspective that one uses to determine if an object is moving.

Explanation:

in physics, a frame of reference, or reference frame, is a perspective that one uses to determine if an object is moving. ... For example, when you see a ball roll down a street, you can tell the ball is moving because the frame of reference is the streets, whatever may be on the side of the roads, or the Earth.

wikipedia

Cystic fibrosis is most common in individuals of Northern European descent, affecting 1 in 3200 newborns. Assuming that these alleles are at Hardy-Weinberg equilibrium, what is the frequency of the disease-causing CFTR alleles in this population

Answers

Answer:

0.0177

Explanation:

Cystic fibrosis is an autosomal recessive disease, thereby an individual must have both copies of the CFTR mutant alleles to have this disease. The Hardy-Weinberg equilibrium states that p² + 2pq + q² = 1, where p² represents the frequency of the homo-zygous dominant genotype (normal phenotype), q² represents the frequency of the homo-zygous recessive genotype (cystic fibrosis phenotype), and 2pq represents the frequency of the heterozygous genotype (individuals that carry one copy of the CFTR mutant allele). Moreover, under Hardy-Weinberg equilibrium, the sum of the dominant 'p' allele frequency and the recessive 'q' allele frequency is equal to 1. In this case, we can observe that the frequency of the homo-zygous recessive condition for cystic fibrosis (q²) is 1/3200. In consequence, the frequency of the recessive allele for cystic fibrosis can be calculated as follows:

1/3200 = q² (have two CFTR mutant alleles) >>  

q = √ (1/3200) = 1/56.57 >>

- Frequency of the CFTR allele q = 1/56.57 = 0.0177  

- Frequency of the dominant 'normal' allele p = 1 - q = 1 - 0.0177 = 0.9823

If you grew up with a family pet, then you have surely noticed that they often seem to hear things that you don’t hear. Now that you’ve read this section, you probably have some insight as to why this may be. How would you explain this to a friend who never had the opportunity to take a class like this?

Answers

Answer:

Force her/him

Explanation:

so that she /he can hear u




2. Explain two different ways that you could classify the following
items: banana, lemon, sandwich, milk, orange, meatball, salad.

Answers

Answer:

Two different ways that you could classify the following  items: banana, lemon, sandwich, milk, orange, meatball, and salad, are the following:

Fruits and not fruits, where the fruits would be banana, lemon and orange; and the non-fruits would be sandwich, milk, meatball and salad.

Food of animal and vegan origin, where the food of animal origin is sandwich, milk and meatball, and the vegan food is banana, lemon, orange and salad.

The following food items can be classified based on either plant or animal origins.

Classification of food products

Food is a substance that contains nutrients that is required for the growth and maintenance of the living organisms.

Food can be gotten from various sources which includes plant and animal sources.

Examples of plant sources are banana, lemon, sandwich, orange and salad

Examples of animal sources are milk and meatball. This is because it can be processed from cow and ram.

Therefore, the following food items can be classified based on either plant or animal origins.

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Two species of closely related frogs are found in the same pond high in the Andes of South America. Both species only have teeth on the top jaw. One has small teeth for holding small live prey prior to swallowing. The other species has very large sharp teeth for injuring and killing large prey prior to biting off pieces of flesh for consumption. The above is an example of:

Answers

Complete question:

Two species of closely related frogs are found in the same pond high in the Andes of South America. Both species only have teeth on the top jaw. One has small teeth for holding small live prey prior to swallowing. The other species has very large sharp teeth for injuring and killing large prey prior to biting off pieces of flesh for consumption. The above is an example of:

Directional selectionAnalogous structuresCharacter displacementHybridization Vestigial structures

Answer:

Character displacement

Explanation:

Competition is an ecological and evolutive process very common in nature. Competition might be intra- or interspecific. Competition between different species in a community or ecosystem might be due to the same resource use, or the same territory, shelter, etcetera. When a resource is useful for two or more species, and limited, they compete to gain it.

The principle of competitive exclusion states that different species with the same requirements sharing the niche can not coexist indefinitely based on the same limited resource. When two competing species coexist, this is because of niche partitioning or niche differentiation.

Differentiation of effective niche is closely related to character displacement.

Character displacement is the result of interspecific competition, in which two or more species that live in the same habitat manage to avoid competition by developing different traits. Morphological divergence, or any adaptative trait development, fixated genetically, is the product of niche segregation. Species tend to differentiate morphologically in the presence of strong competitors. Traits divergence favors coexistence in the same place.

In the exposed example, both species live in the same pond. But to avoid competition and competitive exclusion, species developed different teeth sizes to feed on different prey items.  

You are carrying a case of 24 bottles of water up the stairs to your third-story apartment. What type of contraction are the muscles in your arms experiencing

Answers

Answer:

Concentric contraction.

Explanation:

Concentric contraction is a type of contraction that the muscles in arms experiencing when an individual lifting 24 bottles of water up the stairs. A concentric contraction is a type of muscle activation that creates tension on your muscle as it shortens. As your muscle shortens, it produces enough force to move an object in the upward direction. In weight training we can easily see concentric movement.

What is added to tacos, burritos, and soups that is
high in fiber?

Answers

Answer: flax seed whole wheat tortillas.

1⁄2 cup low-fat refried beans.

1⁄4 cup low-fat cheddar cheese.

leaves lettuce.

1⁄ cup salsa.

1⁄4 cup fat free sour cream.

Pinto beans are added to tacos, burritos, and soups that are high in fiber.

What are fibers and their types?

The body is unable to digest fiber, which is a form of carbohydrate. While fiber cannot be converted into glucose, the sugar that results from the breakdown of most carbs, fiber travels through the body undigested.

The solubility, viscosity, and fermentability of dietary fibers, which have an impact on how they are metabolized by the body, can be used to classify them in general. Dietary fibers have a wide range of chemical compositions.

Fibre comes in two varieties: soluble and insoluble. Both are crucial for good health, digestion, and illness prevention. Thus, Pinto beans are added to tacos, burritos, and soups that are high in fiber.

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i.Name two heart diseases in humans.


ii.State three causes of heart diseases.​

Answers

Answer:

Arrhythmia

Atherosclerosis

Common causes of heart diseases

Diabetes.

Drug abuse.

Excessive use of alcohol or caffeine.

High blood pressure.

A particular cross gives a modified dihybrid ratio of 9:7. What phenotypic ratio would you expect in a testcross of the fully heterozygous F1 crossed with the fully recessive type

Answers

Answer:

3:1

Explanation:

Due to technical problems, you will find the complete explanation in the attached files.

¿Qué nombres reciben los tejidos que dan
origen a los tejidos adultos de las plantas?
a. Tejidos diferenciados
b. Tejidos indiferenciados (meristemos)
c. Tejidos vegetales

Answers

Answer:

D

Explanation:

Which of the cardiac cell characteristics describes the
ability to initiate an electrical impulse?

Answers

Answer:

The correct answer is: automaticity.

Explanation:

Cardiac muscle has several properties. These properties are: automaticity (given by the pacemaker cells), conductivity (meaning that each cardiac cell can transmit the electrical impulse to the next cardiac cells), contractility (like other types of muscles, cardiac muscle cells can contract), and irritability (each cell can contract on its own without the external stimuli).

Cardiac pacemaker cells are the ones with the capacity to initiate the electrical impulse by creating rhythmic impulses called action potentials, and thus directly regulating heart rate.

Pacemaker cells are located in the sinoatrial (SA) node, in the upper portion of the right atrial wall. In these cells, depolarization of the cardiac muscle begins, and the electrical impulse generated by it is transmitted to the atrioventricular (AV) node, the His bundle and then the Purkinje fibers - this order of events is necessary for the correct contraction of the heart to occur. All of these structures are part of the Conduction System of the heart.

7. Shawn plotted the data in a bar graph with two bars: one representing the number of bees per colony in the control cages and the other representing the number of bees per colony in the cages exposed to the fungicide. If the results support the hypothesis that fungicides harm bee development, what would you expect this bar graph to show? Describe the expected pattern in a few sentences.

Answers

Answer:

The first bar increases in length as compared to the second bar.

Explanation:

The length of the bar of control cages in graph increases because the number of bees per colony increases while on the other hand, the length of the bar of the cage that is exposed to fungicides decreases because the bees are negatively affected from the application of fungicides. They act abnormal in behaviour and adversely affected the reproduction of bees that greatly affected its population.

Which of the following are structures of the
lymphatic system? Check all that apply.
Heart
Bone Marrow
Thymus
Spleen
Blood Vessels
Tonsils
Adenoids

Answers

Answer:Bone Marrow

Thymus

Spleen

Explanation:Bone Marrow

Thymus

Spleen

The following are structures of the lymphatic system -

Bone MarrowThymusSpleenTonsilsAdenoids

The lymphatic systemis a network of tissues, vessels, and organs.these structures work together to move a colorless, watery fluid called lymph back into your circulatory system (your bloodstream).The lymphatic system has the following structures:lymph nodes,spleen,thymus the lymphatic tissue found in the small intestine (Peyer's patches)adenoid tonsils,palatinetubal tonsils

Thus, the following are structures of the lymphatic system -

Bone MarrowThymusSpleenTonsilsAdenoids

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Explain how the results from testing Barley High Lysine (BHL) are related to the primary, secondary and tertiary structure of protein?

Answers

Answer:

Enhanced derivatives of barley chymotrypsin inhibitor-2 are known as Barley high lysine (BHL) proteins. BHL has tryptophan, threonine, and isoleucine in a folded structure similar as the tertiary structure of protein.

Derivatives of BHL are digestible in gastric juice and other fluid. Denaturation of these proteins shows proteins in gastric fluid minus pepsin which is looks as the secondary structure of protein. It has four soluble protein groups –

a) Albumins

b) globulin fraction in embryo and scutellary proteins

c) Prolamins

d) Glutelin

Discuss the role of the
kidney in
1. Regulation of blood pressure.
2. Regulation of ECF volume and electrolytes.

Answers

Answer:

blood pressure is regulated by baroreceptors which act via the brain to influence the nervous and the endocrine systems. Blood pressure that is too low is called hypotension, pressure that is consistently too high is called hypertension, and normal pressure is called

Further studies of the genes in the region around the MHC locus identified C4 as a likely causative gene. A particular allele of C4 was significantly associated with schizophrenia. In addition, it was found that individuals with high expression of this allele of C4 had elevated risk of developing schizophrenia. Which of the following variants would be most likely to increase risk of schizophrenia from this allele?

a. a variant that decreases binding of an activating transcription factor to the C4 promoter
b. a 5’ UTR variant that destabilizes the C4 mRNA
c. a duplication of the C4 gene
d. a nonsense coding variant

Answers

Answer:

c. a duplication of the C4 gene

Explanation:

Schizophrenia is a disease that affects the brain, which is characterized by an imbalance of neurotransmitters and the presence of damaged brain tissues. In humans, the Complement component 4 (C4) protein is found at the neurons (i.e., cell bodies, dendrites and axons), which are the most common brain cells, and neuronal synapses. Moreover, gene duplication can increase gene expression when repressive mechanisms (e.g., DNA methylation at promoter sites) capable of silencing the expression of the duplicated gene are lacking. Recently, it has been discovered that a chromosome rearrangement associated with a tandem duplication of a genomic region that contains a C4 gene may contribute to schizophrenia risk by increasing the expression of this gene​. In this example, a, b and d options are associated with a decrease in expression of the C4 gene, either by decreasing its transcription (options a and d) or by posttranscriptional mechanisms (option c).

based on the 1:2:1 proportions of carbon, hydrogen and oxygen, it can be determined that a glucose molecule is a

Answers

Answer:

Carbohydrate (monosaccharide)

Explanation:

Carbohydrates are one of the four biological molecules in nature. Carbohydrates are structurally composed of carbon, hydrogen and oxygen elements. However, these elements (C,H,O) are present in each carbohydrate in the ratio 1:2:1 respectively.

As stated in this question, glucose is an organic molecule made up of carbon, hydrogen and oxygen in the 1:2:1 proportion, hence, it is a CARBOHYDRATE. Since glucose possess one sugar component, it is classified as a MONOSACCHARIDE with a chemical formula C6H12O6.

Explain why virus could not fit into any one of the five kingdom

Answers

Answer:

To make copies of themselves they necessarily need the intervention of a cell. Therefore, viruses are not living beings. That is why they are not included in any Kingdom in which living beings are included

Read each description below regarding the the CNS control of the ANS.

a. It's the integrating centers for reflexes controlling heart rate and blood pressure.
b. It activates the fight-or-flight response.
c. It's the integrating center for thermoregulaton.
d. Sexual thoughts or images can increase blood flow to the genitals.
e. It's the major control center of the ANS.

1. Cerebral Cortex
2. Hypothalamus
3. Brainstem
4. Spinal Cord

Answers

(1)The cerebral cortex, also known as the cerebral mantle, is the outer layer of neural tissue of the cerebrum of the brain in humans and other mammals. The cerebral cortex mostly consists of the six-layered neocortex, with just 10% consisting of allocortex.

(2)The hypothalamus is a small region of the brain. It's located at the base of the brain, near the pituitary gland. While it's very small, the hypothalamus plays a crucial role in many important functions, including: releasing hormones. regulating body temperature.

(3) The brainstem (or brain stem) is the posterior stalk-like part of the brain that connects the cerebrum with the spinal cord. In the human brain the brainstem is composed of the midbrain, the pons, and the medulla oblongata Ten pairs of cranial nerves come from the brainstem.

(4)The spinal cord is a long, fragile tubelike structure that begins at the end of the brain stem and continues down almost to the bottom of the spine. The spinal cord consists of bundles of nerve axons forming pathways that carry incoming and outgoing messages between the brain and the rest of the body.

we should conserve environment give reason​

Answers

Answer:

for healthy living and long life

to save nature and the animals

What is an office what is an office ​

Answers

Answer:

Explanation:

An office can be referred to a building or rooms or a room which is arranged and set up with furnitures and things needed for commercial use or professional work to achieve a goal.

An office is also refered to position of authority occupied by someone to perform some tasks.

Answer:

here's your answer

Explanation:

The office helps in controlling the activities of different people and department of an organisation. Through controlling it ensures that the various activities of business are performed with much accuracy. Memory Center: Office protects important information of past in a safe manner.

Describe the impact of technology on the environmental today

Answers

Explanation:

Other detrimental effects include diseases such as typhoid and cholera, eutrophication and the destruction of ecosystems which negatively affects the food chain. Resource depletion is another negative impact of technology on the environment. It refers to the consumption of a resource faster than it can be replenished

18. Why are enzymes important to cells?
о
They provide structural support.
о
They form the two layers of membranes.
They store large quantities of energy.
о
They help bring about chemical reactions

Answers

Answer:

An enzyme is a type of protein found within a cell. Enzymes create chemical reactions in the body. They actually speed up the rate of a chemical reaction to help support life.

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