If the complex cholesterol- HRP would not be commercially available, use the knowledge you learnt and internet to determine an alternative way for detecting cholesterol.

Answers

Answer 1

Answer:

Classical chemical methods, enzymatic assays, gas chromatography (GC), liquid chromatography (LC), and mass spectrometry (MS)

Explanation:

Classical chemical methods, enzymatic assays, gas chromatography (GC), liquid chromatography (LC), and mass spectrometry (MS) are the alternate methods used for the detection of cholesterol. These methods are used when HRP would not be commercially available for the analysis or detection of cholesterol in the patient's body so these methods can be used for the purpose of detection of cholesterol.


Related Questions

¿Qué afirmación(es) es(son) correcta(s)?:
a) El dióxido de azufre de la quema de carbón en centrales
eléctricas protege contra la lluvia ácida.
b) Los óxidos de nitrógeno de las emisiones de combustión
provocan el aumento del pH en el agua de lluvia.
c) Una solución ácida de dióxido de carbono en agua de lluvia se
llama lluvia ácida.
d) El dióxido de azufre junto con los óxidos de nitrógeno son las
principales causas de la lluvia ácida.

Answers

Explanation:

jwjejeueuru4ibwsbbefbrvbrbebdbfhhdhdhdbdbdfbd3 eebdddd

a special effects artist mixes two different liquids in a bowl. both liquids are white. when she heats the bowl, a new compound forms. will the new compound be a white liquid?

Answers

Answer:

if a special effects artist mixes two different liquids in a bowl and they both are too white !

after heats those liquids

its forms curd like substance

its either will be white, its can increase its colour as darkish white or lightly yellowishblack

when she heats the bowl, a new compound forms and it will be white before heating and after heating it may be white or other dark color.

What are the properties of a compound ?

A compound composed up of two or more elements which are chemically combined with a fixed proportion by their mass.

For example, Water, Sodium Chloride,  Ammonium Chloride etc.

They have fixed melting and  boiling points.

Formation of compound is a change in chemical reaction and the components of compound are mixed in a fixed proportion.

Two major types of compounds are covalent and ionic compounds.

Covalent compounds refers to formation of covalent bond among two nonmetals, like water or methane. These molecules are neutral and weak.

When metal react with non metal it form Ionic compounds, are held together by opposite charges, so the bond is stronger than covalent compound.

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Human being get energy from

Answers

Are you asking how humans get energy?
Any food they consume or sleep. Hope this helps

What is the function of the mitochondria?
A. Stores the cell's DNA
B. Builds proteins
C. Produces energy for the cell by respiration
OD. Stores the cell's glucose
Reset Selection

Answers

C. Mitochondria is like the power plant of the cell and produces its energy.

Answer:

Produces energy for the cell by respiration

Explanation:

The glucose obtained from food is broken down to pyruvic acid in the cytoplasm. This pyruvic acid is broken down into oxygen, water and energy rich ATP molecules in the Mitochondria.

Why should body temperature not be allowed to fluctuate too much? ​

Answers

Answer:

Because that can destroy the helpful enzymes in the body, and therefore cause a lot of problems or maybe cause death

What is a community?
1 all the animals that live in a habitat
2 a single species that lives in a habitat
3 all the species that live in a habitat
4 a population that lives in a single habitat

Answers

Answer:

3. All the species that live in a habitat.

A community is where all the species live in a habitat. Hence the correct option is 1.

A community is an ecological term that encompasses all the different species of organisms that coexist and interact within a specific habitat or geographic area. It includes plants, animals, fungi, and microorganisms that share the same environment and form intricate ecological relationships with each other.

These relationships can be competitive, predatory, symbiotic, or other forms of interactions that influence the dynamics and structure of the community. Understanding the composition and interactions within a community is vital in studying the biodiversity, ecosystem functioning, and overall health of a given habitat.

Hence the correct option is 1.

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You are studying an enzyme that is inactivated by phosphorylation and create a mutant in which the threonine that is normally phosphorylated is replaced with glutamate. Predict the impact of this change on the activity of this enzyme. Group of answer choices

Answers

Answer:

always active  

Explanation:

Phosphorylation is a posttranslational modification that consists of the addition of phosphate groups to specific amino acids on the protein. Phosphorylation acts as a molecular switch for proteins that are phosphorylated (i.e., in some situations phosphorylation acts to activate protein function, whereas in other situations phosphorylation can inactivate protein function). Phosphorylation modifies the three-dimensional structure of the protein, thereby affecting, for example, the accessibility of the active site of a phosphorylated enzyme to its substrate. Phosphorylation can occur only at the side chains of three amino acids: Serine, Threonine and Tyrosine. In this case, the enzyme is inactivated by phosphorylation on the Threonine residue, so it is expected that the mutant enzyme cannot be phosphorylated, remaining in an active state.

Ecosystems rely on interdependence between species to keep balance. Which of the following is a threat to a stable
ecosystem?
A. Loss of biodiversity
B. High biodiversity
C. Low biodiversity
D. Increase in biodiversity

Answers

Answer:

loss of biodiversity

Explanation:

Biodiversity- refers to the variety of life on Earth at all its levels, from genes to ecosystems, and can encompass the evolutionary, ecological, and cultural processes that sustain life.

loss in biodiversity affect food chains greatly

thanks

hope it helps

The conversion of liquid water into gaseous water is called?

Answers

Answer:

vapourization

Explanation:

When the water is heated, it changes into water vapour which is called vapourization or sometimes we can also call it evaporation.

Amoeba sisters video recap Biomagnification

Answers

I suggest you watch the Amoeba Sisters video again, 1-2x and take notes.

Biomagnification is when toxins / heavy metal are present in small amounts in fish, but accumulate as you move into higher trophic levels. This is one reason why it can be toxic for humans to eat tertiary or quaternary consumers, such as swordfish, shark, etc.

Biomagnification refers to the presence of higher concentration of chemical toxins as a result of the accumulation of toxins in organisms.

What is biomagnigication?

Biomagnification is best explained as a condition in which the chemical concentration of a toxin is amplified in an organism compared to the environment in which the organism is found.

Biomagnification usually is observed as one goes higher in the trophic levels of organisms.

For example, chemical pollutant found in water may be present at tolerable levels. However, in organisms, living in the water, the concentration of the pollutant is higher as these organisms accumulate these toxins in their tissues.

Therefore, biomagnification refers to the accumulation of toxins in organisms higher than found in their environment.

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functions of insulin

Answers

Insulin is a hormone that controls blood glucose (sugar) levels by signaling the liver and muscle and fat cells to take in glucose from the blood. Insulin therefore helps cells to take in glucose to be used for energy. If the body has sufficient energy, insulin signals the liver to take up glucose and store it as glycogen.

Answer:

Insulin helps control blood glucose levels by signaling the liver and muscle and fat cells to take in glucose from the blood. Insulin therefore helps cells to take in glucose to be used for energy. If the body has sufficient energy, insulin signals the liver to take up glucose and store it as glycogen.

Explanation:

What variable should Anurag change in his experiment

Answers

Answer:

For geological carbon sequestration, the reaction of aqueous CO2 with silicate rock permits carbonate formation, achieving permanent carbon sequestration.A

Explanation:

Please help me with these questions
I will mark the Brillianest​

Answers

Answer:

[tex] \pink{\qquad \boxed{\boxed{\begin{array}{cc} \maltese \: \: \: \bf \: see \: the \: picture \: \: \end{array}}}}[/tex]

what is the definition of wildlife?

Answers

Answer:

wild animals collectively; the native fauna (and sometimes flora) of a region.

Explanation:

I hope this helped :)

Animals living in their natural habitat and not within the possession or control of humans.

Carnivore that feeds on primary consumers
Question 3 Multiple Choice Worth 3 points
(01.01 LC)
Which of the following is an example of a decomposer?

Answers

we need the options in order to answer

Our body needs both vitamin and mineral in a small quantity ,still they are important why?​

Answers

Answer:

Vitamins and minerals are considered essential nutrients—because acting in concert, they perform hundreds of roles in the body. They help shore up bones, heal wounds, and bolster your immune system. They also convert food into energy, and repair cellular damage.

Organisrns that transfer diseases to hurnans are
O hosts
O pathogens
O parasites
O vectors

Answers

Parasites is correct

Phân tích các quy luật hoạt động thần kinh cấp cao ở trẻ và vận dụng trong thiết lập thói quen học tập và kỉ luật ở học sinh tiểu học.

Answers

Answer:

very different than ducks do u want it is not the

The meaning of ALARA in radiation?

Answers

Answer:

The guiding principle of radiation safety is “ALARA”. ALARA stands for “as low as reasonably achievable”.

i need help in biology questions please G10?

Answers

Answer:

ok where is it

we can help only if there is something attached

Attach the picture or something so we could respond

what is haemopoiesis??​

Answers

Haemopoiesis is from greek meaning “ to make. new blood” •

Explanation:

It refers to the formation of blood cellular. components.

The most basic organization level of life is a ____________. A. membrane B. tissue C. cell D. organ

Answers

[tex]Hello[/tex] [tex]There![/tex]

The answer is...

C. Cell.

Hopefully, this helps you!!

[tex]AnimeVines[/tex]


What component of Earth's atmosphere exists entirely as a result of photosynthesis?
oxygen pas
n mas
O water vapor
O nitrogen gas
O carbon dioxide gas

Answers

Answer:

Explanation:

Carbon dioxide

The last one is correct
Hope its help for your questions

Question 5
Not yet answered
Marked out of 1.00
P Flag question
Complete the following sentence: "The interior of living cells is more
than the exterior because more
ions are expelled than ions are taken in by the sodium-potassium pump."
Select one:
O a. electropositive. Nak
O b. electronegative, Na.
O C. electronegative, Na, K
O d. electropositive, Na+, K+
Question 6
Not yet answered
Marked out of 1.00
P Flag question
The high concentration of protons in the inner mitochondrial space relative to the mitochondrial matrix represent
O
В.
77°F
AGD (0)
10-25 PM
7/28/2021

Answers

Answer:

i really really need the brainly points

Explanation:

sry for this answer, i need the answers for myself

The mass of the Sun is 1.99 × 1030 kg. Jupiter is 7.79 × 108 km away from the Sun and has a mass of 1.90 × 1027 kg. The gravitational force between the Sun and Jupiter to three significant figures is × 1023 N.

Answers

Answer:

4.16

Explanation:

edge2021

Answer:

4.16

Explanation:

Occurs in mitochondria.
Select one:
a. Both Photosynthesis and Cellular Respiration
b. Neither Photosynthesis nor Cellular Respiration
c. Photosynthesis
d. Cellular Respiration

Answers

Answer:

d. cellular respiration

Explanation:

mitochondria is where cellular respiration occurs, and photosynthesis occurs in the chloroplast, so d would be the most reasonable answer.

A man bought a goldfish in a pet shop. Upon returning home, he put the goldfish in a bowl of recently boiled water that had been cooled quickly. A few minutes later the fish was found dead. Explain what happened to the fish

Answers

Answer:

lack of oxygen in the water

Explanation:

The fish most likely died from lack of oxygen in the water. This is because fishes actually use their gills to extract and breathe in the oxygen from the water while also expelling carbon dioxide from their lungs. Similar to how humans breathe. When the water was boiled it caused the dissolved gases to be expelled, which includes oxygen. Therefore, without the necessary oxygen in the water, the fish ultimately suffocated.

Boiling the water reduce its amount of dissolved oxygen which is needed by the fish to breathe, that's why the fish died after few minutes.

What is dissolved oxygen?

Dissolved oxygen is the amount of oxygen present in the water.

The organisms live to consume dissolved oxygen to breathe.

The amount of dissolved oxygen is high in the current water like rivers than in the still water like pond.

If the amount of DO is high in the water, it causes bubble gas disease in the aquatic organisms.

If the amount of DO is low in the water than, fishes and other aquatic organism cant survive due to low oxygen level.

Thus, boiling the water reduce its amount of dissolved oxygen which is needed by the fish to breathe, that's why the fish died after few minutes.

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how much water was retained by soil C​

Answers

Answer:

we dont know sorry but i dont know

QUESTION 11 1. Through PCR, we have determined the PER3 genotypes for a class of students as follows: H4/H4 = 125 individuals; H4/H5 = 85 individuals and H5/H5=24 individuals. Using the allele's frequencies experimentally derived, calculate the frequency of the H4/H5 genotype that would be expected if the class were a population in Hardy-Weinberg equilibrium. 1. 0.28 2. 0.51 3. 0.19 4. 0.72 5. 0.14 6. 0.24 7. 0.41 QUESTION 12 1. Through PCR, we have determined the PER3 genotypes for a class of students as follows: H4/H4 = 125 individuals; H4/H5 = 85 individuals and H5/H5=24 individuals. Using the genotype frequencies derived assuming that the class were a population in Hardy-Weinberg equilibrium, calculate the number of H4/H4 individuals that would be expected in the class (rounded numbers). 1. 19 2. 57 3. 72 4. 147 5. 171 6. 120 7. 96 QUESTION 13 1. Through PCR, we have determined the PER3 genotypes for a class of students as follows: H4/H4 = 125 individuals; H4/H5 = 85 individuals and H5/H5=24 individuals. Considering the Hardy Weinberg equilibrium and comparing the observed and the expected number of individuals for the three genotypes, calculate the value of the Chi-square statistic 1. 2.69 2. 0.05 3. 28.67 4. 14.59 5. 0.50 6. 22.31 7. 3.84 QUESTION 14 1. Through PCR, we have determined the PER3 genotypes for a class of students as follows: H4/H4 = 125 individuals; H4/H5 = 85 individuals and H5/H5=24 individuals. Considering the Hardy Weinberg equilibrium, which is the (correct) null hypothesis tested by Chi-square? 1. The whole class represents a population that is in Hardy-Weinberg equilibrium 2. The whole class represents a population that may not be in Hardy-Weinberg equilibrium 3. The whole class represents a population that is not in Hardy-Weinberg equilibrium 4. The whole class represents a population that may be in Hardy-Weinberg equilibrium QUESTION 15 1. Through PCR, we have determined the PER3 genotypes for a class of students as follows: H4/H4 = 125 individuals; H4/H5 = 85 individuals and H5/H5=24 individuals. Considering the Hardy Weinberg equilibrium and calculating the Chi-square statistic, do you reject or fail to reject the null-hypothesis? 1. Comparing the value of Chi-square that I calculated with the critical value of Chi-square for the appropriate degrees of freedom I conclude that P>0.05. Hence, I reject the null hypothesis. 2. Comparing the value of Chi-square that I calculated with the critical value of Chi-square for the appropriate degrees of freedom, I conclude that P>0.05. Hence, I fail to reject the null hypothesis. 3. Comparing the value of Chi-square that I calculated with the critical value of Chi-square for the appropriate degrees of freedom I conclude that P<0.05. Hence, I reject the null hypothesis. 4. Comparing the value of Chi-square that I calculated with the critical value of Chi-square for the appropriate degrees of freedom I conclude that P<0.05. Hence, I fail to reject the null hypothesis.

Answers

According to Hardy-Weinberg, when a population is in equilibrium, it will have the same allelic frequencies generation after generation, meaning that they are stable, they are not evolving.

When any evolutive force is acting on the population, this equilibrium breacks, and allelic and genotypic frequencies change through generations, differing from the expected ones.  

A) Option 7 is the correct answer ⇒ 0.41

B) Option 6 is the correct answer ⇒ 120

C) Option 7 is the correct answer ⇒ 3.84

D) Option 1 is the correct answer ⇒ The class represents a population that is in H-W equilibrium

E) Option 1 is correct. Comparing the value of Chi-square that I calculated with the critical value of Chi-square for  the appropriate degrees of freedom I conclude that P>0.05. Hence, I reject the null hypothesis.

-------------------------------------------

Allelic frequencies in a locus are represented as p and q, referring to the

allelic dominant or recessive forms. The genotypic frequencies after one generation are p² (H0m0zyg0us  dominant), 2pq (H3ter0zygous), (H0m0zyg0us recessive). Populations in H-W equilibrium will get the same

allelic frequencies generation after generation.

The sum of the allelic frequencies equals 1, this is p + q = 1.

In the same way, the sum of genotypic frequencies equals 1, this is

p² + 2pq + q² = 1

Being

 p the dominant allelic frequency,

 q the recessive allelic frequency,

 p² the h0m0zyg0us dominant genotypic frequency

 q² the h0m0zyg0us recessive genotypic frequency

 2pq the h3ter0zyg0us genotypic frequency

 

Situation: Through PCR, we have determined the PER3 genotypes for a class of students as follows:

 H4/H4 = 125 individuals;

 H4/H5 = 85 individuals;

 H5/H5=24 individuals.

⇒ Total number of individuals= 125 + 85 + 24 = 234

⇒ Genotypic frequencies, F(xx):

 F(H4/H4) = 125/234 =0.534

 F(H4/H5) = 85/234 = 0.363

 F(H5/H5) = 24/234 = 0.102

⇒ Allelic frequencies, f(x):

 f(H4) = p = F(H4/H4) + 1/2 F(H4/H5) = 0.534 + 0.363/2 = 0.534 + 0.182 = 0.716

 f(H5) = q = F(H5/H5) + 1/2 F(H4/H5) = 0.102 + 0.363/2 = 0.102 + 0.182 = 0.284

Questions:

A)  According to the theoreticall frame, we know that 2pq is the h3ter0zygous genotypic frequency. So,  

F(H4/H5) = 2pq = 2 x 0.716 x 0.284 = 0.408 ≅ 0.41 ⇒ Option 7 is the correct answer.

-------------------------------------------------------------------------------------------------------------

B)  According to the theoreticall frame, we know that p² is the h0m0zyg0us genotypic frequency. So,

p = 0.716

p² = 0.5126 ≅ 0.513 ⇒ This is the genotypic frequency.

To calculate the number of individuals carrying this genotype, we need to multiply it by the total number of

individuals.

H4/H4 individuals = p² x total number of individuals = 0.513 x 234 = 120

Option 6 is the correct answer.

-----------------------------------------------------------------------------------------------------------

C)  Up to here we know that 2pq = 0.41 and p² = 0.513

Now we need to calculate q ²

q = 0.284, then q² = 0.284² = 0.08

These are the expected frequencies if the population was in H-W equilibrium.

The expected number of individuals with each genotype are:

 H4/H4 = 0.513 x 234 = 120 individuals

 H4/H5 = 0.41 x 234 = 96 individuals

 H5/H5= 0.08 x 234 = 18 individuals

The observed number of individuals with each genotype are:

 H4/H4 = 125 individuals

 H4/H5 = 85 individuals

 H5/H5=24 individuals

X² = ∑ (Observed - Expected)²/Expected)

X² = ((125-120)²/120) + ((85 - 96)²/96) + ((24-18)²/18)

X² = 0.21 + 1.26 + 2 =

X² = 3.47

The clossest option is option 7 = 3.84. The difference might be related to decimals and rounding.

-------------------------------------------------------------------------------------------------------------

D)  The correct answer is  1 ⇒ The whole class represents a population that is in Hardy-Weinberg equilibrium

The null hypothesis always predict that populations are in H-W equilibrium.

-----------------------------------------------------------------------------------------------------------

E)  

 X² = 3.47

 Freedom degrees = n - 1 = 3 - 1 = 2

 Table p value: 7.82

 Significance level, 5% = 0.05

 Table value/Critical value = 5.991

5.991 > 0.347

Meaning that the difference between the observed individuals and the expected individuals is statistically  significant. Not probably to have differe by random chances. There is enough evidence to reject the null

hypothesis.

Option 1 is correct. Comparing the value of Chi-square that I calculated with the critical value of Chi-square for  the appropriate degrees of freedom I conclude that P>0.05. Hence, I reject the null hypothesis.

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For every 100ml of deoxygenated blood delivers approximately _____ml of CO2 to the alveoli.​

Answers

Answer:

For every 100ml of deoxygenated blood delivers approximately __4___ml of CO2 to the alveoli.

Answer:

4ml

Explanation:

For every 100ml of deoxygenated blood delivers approximately 4 ml of CO2 to the alveoli.

Hope it is helpful....
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I need help asap please How do simple organisms exchange substances?O A. Directly across the alveoli.OB. Through spiracles.C. Directly across the external membranes.D. Through specialized gas exchange systems. Three important nutritional additions to training for a long distance race areO ProteinO WaterO Increase caloriesO All of the answer choices find the surface area of the prism HURRY HURRY 14feet 23feet round each measurement to the nearest ten What process must happen for clouds to form? Mrs. Helton is making gift bags as prizes for her math classes. She has 30 little bags of Hershey Kisses and 15 Blow Pops. What is the greatest number of gift bags she can make if all gift bags are the same, and she does not want any left over? Y= 3x-12x+6=y substitution method (4m-n-3). 12n138m 20 1-2. Roel earns P 8,000 per month as basic salary. In addition, he earned P 5,725.50 as commission and P 852.50 as overtime pay in January. How much did he earn that month?3-4. A biscuit factory originally packaged 0.65kg of crackers. To increase profitability, the company reduced the weight by 0.035 kg with a more affordable price. What is the new weight per pack of crackers? it was raining .........( heavy/heavily) como se llama el primer cartlago de la trquea Which groups' main disagreement at the framing was over the basis of representation in Congress?Group of answer choicessmall states and large statespopulists and elitistsstates' rights advocates and advocates for strong national governmentthe North and the South 1. Find the ratio of of 25cm and 1m .2. Find the product of (a + 4)(a 4). Question 7 of 20The Supreme Court has recently ruled that a federal lawoutlawing the burning of the American flag in protestviolates the First Amendment's guarantee of free speech.The Court delivered a split decision, with five justicesvoting to overturn the law and four voting to uphold it.How could one of the major organizations within the federal governmentmost effectively overcome the Supreme Court's ruling to allow the federalgovernment to outlaw flag burning?A. Federal district and appellate judges could refuse to follow theSupreme Court's precedent.B. The federal bureaucracy could refuse to comply with the SupremeCourt's orders.C. Congress could propose a constitutional amendment that wouldoutlaw flag burning.D. The president could vold the Supreme Court's ruling using her vetopower.SUBMIT Which table represents a linear function? please help !!!! i would really appreciate it What is the role of syntax in this line?At this, after a second in which his head made the movement of a baffled dog's on a scent and then gave a frantic little shake for air andlight, he was at me in a white rage, bewildered, glaring vainly over the place and missing wholly, though it now, to my sense, filled theroom like the taste of polson, the wide, overwhelming presence.(from The Turn of the Screw by Henry James)OA. parodying archalsmsOB. describing actionOcclarifying meaningOD. intensifying horror QUESTION 2A board is 86 cm. in lenght and must be cut so that one piece is 20 cm. longer than the other pieceFind the lenght of each piece.A26 cm and 60 cmb. 33 cm and 53 cmC 30 cm and 56 cmd. 70 cm and 16 cm determine the general term of this sequence -15:-11;-7;,,173