In a diploid cell, a FISH probe should produce ____________ signals in metaphase FISH and ____________ signals in interphase FISH.

Answers

Answer 1

Answer: Four, two

Explanation:

FISH (fluorescence in situ hybridization) is a technique for chromosome mapping with probes that emit fluorescence and allow the visualization, distinction and study of chromosomes of nuclei in metaphase or inferophase. In this way, any type of anomaly such as inversions, aneuploidies, duplications or microdeletions can be seen. To do this, a non-specific DNA staining is performed with DAPI (a fluorophore that marks the nucleus where DNA is present) and the fluorescence will be seen under the microscope.

When cells are in metaphase, their chromosomes are condensed in preparation for cell division. To stop them in metaphase, colchicine is used which is a microtubule depolymerizing agent responsible for separating the sister chromatids of a chromosome, thus preventing the progression of cell division and arresting the cell cycle in metaphase. Here we will see translocations, microdeletions or duplications.

Interphase FISH is used to see decondensed chromatin, which allows higher resolution in detecting small abnormalities. It is mainly used for the detection of aneuploidies or large deletions, duplications or translocations when samples are difficult to grow or are scarce, as in the case of fetal or tumor cells. It is not possible to distinguish between a normal karyotype and a karyotype showing a balanced translocation. In addition, it can be used in the analysis of solid tumors, which divide very infrequently.

During metaphase, the chromosomes are duplicated so there would be two sets of chromosomes (a total of four chromosomes). Then, there would be four signals for each probe. During interphase, the chromosomes are not dupicated. Since it is a diploid organisms, there would be two chromosomes and this will represen two signals for probe.


Related Questions

7. Which substances are produced by the hydrolysis of an
oil?
(1) water and nucleic acids
(2) amino acids and dipeptides
(3) fatty acids and glycerol
(4) glucose and water

Answers

Answer:

3) Fatty acids and Glycerol

Name the organ of locomotion of englena and chlamydomonas

Answers

Answer:

Paramecium:The primary locomotory organ is cilia which is covers the whole body of paramecium. They also have flagella as its secondary locomotary organ. Euglena: They have flagella and they move by beating up the flagella. Chlamydomonas :A flagellum is a long, whip-like projection of a cell made up of microtubules.

Which characteristics distinguish a law from a
theory? Check all that apply

Answers

a theory is an idea that has yet to be proven by experimentation. A law is a theory that has been proven correct.

What produces two fertility hormones, Follicle stimulating hormone and luteinizing hormone?
A) Testes
B) Pituitary
C) pancreas
D) Ovaries

Answers

[tex]\underline \bold{ \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: }[/tex]

Question:

What produces two fertility hormones, Follicle stimulating hormone and luteinizing hormone?

A) TestesB) PituitaryC) pancreasD) Ovaries

Answer:

[tex]\huge\quad\quad \underline{ \boxed{ \red{\sf{b.)\:Pituitary}}}}[/tex]

Explanation:

Did you know that Pituitary produces two fertility hormones, Follicle stimulating hormone and luteinizing hormone.

[tex]\underline \bold{ \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: \: }[/tex]

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What is the correct chronological order of the steps leading to protein synthesis?
rRNA, mRNA, DNA, protein
mRNA, DNA, rRNA , protein
DNA, mRNA, rRNA, protein
rRNA, DNA, mRNA, protein

Answers

Answer:

the third option is the right one.

Explanation:

m RNA gets its information from DNA and then sends it to rRNA which will later turn the message into a protein.

hi can u Help me in my hw, Define the following terms:1)Epicenter 2)Seismograph(don't answer if u don't know the answer plz)​

Answers

Answer:

Epicenter is the point on Earth's surface directly above the focal point of the earth quake.

Actually it's above the hypocenter.

Seismograph: Is an instrument that records the seismic waves caused by action of earth quake effect.

The second generation of purebred cross of -hom-ozygo us recessive organism, with a —hom-ozygous dominant organism, contain a ratio of approximately _______ expressed dominant traits to recessive traits.
A. 2:1
B. 1:1
C. 1:3
D. 3:1

Answers

Answer:

D. 3:1

Explanation:

Gregor Mendel, in one of his numerous studies, performed a cross between purebred dominant (TT) pea plants and purebred recessive (tt) pea plants. All the offsprings of this cross appeared heterozygous tall (Tt) in the F1 generation.

However, when he self-crossed the F1 generation i.e. Tt × Tt, Mendel obtained a phenotypic ratio of 3 tall plants (dominant traits) : 1 short plant (recessive). This showed that the masked gene (recessive) was still present.

Raw materials required in the autotrophic mode of nutrition involve:​

Answers

Answer:

Water,mineral salts and carbon dioxide

All parts of a nonvascular
plant must be near a
because it doesn't have
long roots.

A. water source
B. heat source
C. food source
D. air source

Answers

Answer:

a.water source

Explanation:

Please help me ????!!!!

Answers

Answer:

1. Form Bonds 2. True

Explanation:

What are the two main phases of the cell cycle

Answers

Answer:

interphase and the mitotic (M) phase.

Explanation:

The stages of the cell cycle are divided into two major phases: interphase and the mitotic (M) phase.

Answer:

The interphase & the miotic phase

Explanation:

QI the father
13. A father and mother claim they have been given the wrong baby at the hospital. Both parents are
blood type A. The baby they have been given is blood type o. Does the genetic evidence support
their claim?

Answers

No, the claim is not based on scientific evidence because to heterozygous parents with genotype AO may have offspring with O phenotype and therefore OO genotype (proportion 1/4 of descendence).

What is the ABO blood type?

The ABO blood type is a group of phenotypes controlled by a single locus, which is based on the alleles that encode surface antigens in red blood cells. The O phenotype is due to the recessive allele, while the A allele is dominant and therefore masks the O allele in heterozygous individuals.

Therefore, we can conclude that parents having the AO genotype may produce offspring with OO genotype

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PLEASE HELP ASAP FAST

Answers

Answer:

B.UAA is correct answer

Describe three different measures that can be obtained from a single age distribution graph comparing males and females of a given population.

Answers

Measurements of height weight and age

A single age distribution graph comparing males and females of a given population can provide a wealth of information. Three of the most important measures that can be obtained from such a graph are the population size, the age structure, and the gender ratio.

What is the way to measure the population size ?

The population size can be determined by adding the total number of males and females represented in the graph. This is an important measure of the overall population size, and can be used to compare the population size of different countries or regions.

The age structure of the population can be determined by looking at the distribution of males and females across different age groups. This is an important measure of the demographic makeup of a population, and can be used to identify trends in population growth or decline.

Finally, the gender ratio of the population can be determined by comparing the number of males and females in each age group. This is an important measure of the overall gender balance in a population, and can be used to identify areas where there is a need for greater gender equality.

In conclusion, a single age distribution graph comparing males and females of a given population can provide a wealth of information. Three of the most important measures that can be obtained from such a graph are the population size, the age structure, and the gender ratio.

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What are micronutrients and why do we need them?

Answers

Explanation:

Micronutrients are one of the major groups of nutrients your body needs. They include vitamins and minerals. Vitamins are necessary for energy production, immune function, blood clotting and other functions. Meanwhile, minerals play an important role in growth, bone health, fluid balance and several other processes.

if my answer helps you than mark me as brainliest.. thanku

Is the podocyte process of the glomerular capillary wall the same as the endothelial layer?

Answers

Answer:

The podocytes have long foot processes called pedicels, for which the cells are named (podo- + -cyte). The pedicels wrap around the capillaries and leave slits between them. Blood is filtered through these slits, each known as a filtration slit or slit diaphragm or slit pore.

explain how typical cells are adapted to their function​

Answers

Answer:

Many cells are specialized. They have structure that are adapted to their function. For eg : muscle cells bring parts of the body together. They contain protein fibres that can contract when energy is available, making the cells shorter.

What is conjugation?

A. when two haploid cells fuse and reproduce
B. when two organisms exchange information and reproduce
asexually through mitosis
C. when a cell divides and produces two identical cells

Answers

Answer:

when two organisms exchange information and reproduce

Marianela takes a huge drink of her coffee, assuming that it is at a tolerable temperature, and the heat sears her mouth. Although the pain is so great that her reflex is to spit out the coffee, she does not do so. The reflex to spit out the coffee is inhibited at the

Answers

Answer:

This question lacks options, options are:

A) glial cell

B) interneuron

C) sensory neuron

D) motor neuron.  

The correct answer is D.

Explanation:

The brain sends instructions to the muscles that control voluntary movement through nerve cells called motor neurons. The function of the motor neuron is basically to carry the information that comes from the descending pathways of the spinal cord, in order to generate an activation in the tissues of the skeletal and visceral muscles of all parts of the body. Motor neurons need the information sent to them by sensory or efferent neurons. Since in order to perform the appropriate muscle movements for the situation, our brain must receive information from the outside. By sending sensory stimulation through interoreceptors, motor neurons can regulate visceral activity, stimulating or inhibiting the activity of their effector tissues. In this way, our nervous system integrates the information from both types of neurons and allows us to move and react according to the demands and circumstances of our external context.

What allows humans to have different traits from each other?
A. They store DNA in different places.
B. They have different genes.
C. They have different haploidnumbers.
gling
D. They have different number
Ichromosomes.
SUBMIT

Answers

Answer:

B. They have different genes.

Explanation:

Which of the following controls the involuntary actions in the body​

Answers

medulla oblongata

Involuntary actions are under the voluntary control of the medulla oblongata which is generally referred to as the midbrain. The involuntary movements are characterised by relatively slower speed. The best example of this movement is the beating movement of the human heart.

What would most likely happen if the number of shrimp decreased?
A. The plankton population would increase.
B. The salmon population would increase.
C. The salmon population would not change.
D. The plankton population would not change.

Answers

Answer:

A.

Explanation:

Shrimp eat plankton, so, if the number of shrimp decrease, the plankton will increase. hope I helped, and God bless you!

which climate does sparrow live hot or cold

Answers

Answer:

Hot ok please give me brilliance

Explanation:

please

what is photosynthesis​

Answers

Answer:

The process of making food by Green plants (autotroph) is called photosynthesis. For performing photosynthesis water, sunlight and carbon dioxide are used by the plants.

They take sunlight from sun, water from soil and carbon dioxide gas from the air present around us. The leaves have small holes called stomata which allows air to enter the plant. Leaves also contains chlorophyll in them which traps sunlight and helps in making food. Roots of plants absorbs water and Minerals from the soil and transfers them leaves through stem.

The chemical equation for the photosynthesis

is -

carbon dioxide + water -> glucose + oxygen + energy

[tex] \huge \boxed{\mathfrak{Question} \downarrow}[/tex]

What's photosynthesis?

[tex] \large \boxed{\mathfrak{Answer \: with \: Explanation} \downarrow}[/tex]

Photosynthesis is the process in which green plants derive their food (nutrients and water) through the capturing of light energy (sunlight) and using it to convert carbon dioxide (CO2) and water (H2O) into oxygen (O2) and other substances (compounds) that are rich in energy. Chlorophyll a green pigment present in plants is used in this process.

__________________

Formula ⟶ [tex]\boxed{\bf\:6CO_2 + 6H_2O \rightarrow C_6H_{12}O_6 + 6O_2} [/tex]

__________________

So, the products of photosynthesis are [tex]\large\boxed{\boxed{\bf\:C_6H_{12}O_6 + 6O_2}}[/tex]

Which part of plant carries water and minerals from roots to other parts of the plant

Answers

Answer: The xylem distributes water and dissolved minerals upward through the plant, from the roots to the leaves. The phloem carries food downward from the leaves to the roots. Xylem cells constitute the major part of a mature woody stem or root.

The data table and the cladogram below provide information about the evolutionary history of several different species of animal. Using this information, which of the following is true of trout?

They are more closely related to salamanders than they are to birds.

They are more evolved than salamanders and lizards.
They evolved into a separate species due to the presence of lungs.
They have jaws and lungs but lack claws or nails, feathers, and mammary glands.

Answers

Answer:

They are more closely related to salamanders than they are to birds.

Explanation:

According to the data, trout are more closely related to salamanders than birds.

The first data table shows how the salamander only has one extra trait that the trout does not have (lungs).

On the other hand, birds have three extra traits that the trout doesn't have (claws/nails, feathers, and lungs).

The cladogram further shows how trout are more closely related to salamanders compared to birds.

Trout and the salamander are closer together on the cladogram, while the bird is further away. This means they are more related on the evolutionary timeline.

So, the correct answer is that They are more closely related to salamanders than they are to birds.

The statement which is true about Trout is as follows:

They are more closely related to salamanders than they are to birds.

Thus, the correct option is A.

What is Cladogram?

A Cladogram may be defined as a type of family tree or classification tree which is based on evolutionary relationships. It is also known as a phylogenetic tree.

Trout and the salamander are positioned closer to one another on the cladogram while the bird is being far away. The presence of lungs is marked from the salamander, so trout do not have lungs.

Trouts evolved from Hagfish but not from salamanders and lizards. Trouts only have jaws but lungs, claws or nails, feathers, and mammary glands.

Therefore, the correct option for this question is A.

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1. Sensation is to as perception is to a. vision, olfaction b. conscious, unconscious c. awareness, interpretation d. taste, vision.​

Answers

Answer:

opt (c)

Explanation:

HELP ASAP I WILL GIVE BRAINLIST

The picture shows two containers filled with a gas, both initially at room temperature.

Which statement is correct?

The gas particles in both containers have the same average kinetic energy because they have equal number of particles.

The gas particles in both containers have the same average kinetic energy because they have the same volume.

The average kinetic energy of the gas particles is greater in container A because it has a lower temperature.

The average kinetic energy of the gas particles is greater in container B because its particles move faster.

Answers

Answer:

1) CO₂

2) 0.2551 g

Explanation:

The balanced reactions are:

CaCO₃ + 2HCl → CaCl₂ + H₂O + CO₂

MgCO₃ + 2HCl → MgCl₂ + H₂O + CO₂

1) The gas produced is CO₂.

2) Calculate mass of CaCO₃:

(0.5236 g) (0.4230) = 0.2215 g CaCO₃

Convert to moles:

(0.2215 g CaCO₃) (1 mol / 100.1 g) = 0.002213 mol CaCO₃

Find moles of CaCO₃:

(0.002213 mol CaCO₃) (1 mol CO₂ / mol CaCO₃) = 0.002213 mol CO₂

Convert to mass:

(0.002213 mol CO₂) (44.01 g / mol) = 0.09738 g CO₂

Calculate mass of MgCO₃:

(0.5236 g) (0.5770) = 0.3021 g MgCO₃

Convert to moles:

(0.3021 g MgCO₃) (1 mol / 84.31 g) = 0.003583 mol MgCO₃

Find moles of MgCO₃:

(0.003583 mol MgCO₃) (1 mol CO₂ / mol MgCO₃) = 0.003583 mol CO₂

Convert to mass:

(0.003583 mol CO₂) (44.01 g / mol) = 0.1577 g CO₂

Total mass of CO₂:

0.09738 g CO₂ + 0.1577 g CO₂ = 0.2551 g CO₂

Answer:

The average kinetic energy of the gas particles is greater in container B because its particles move faster.

Explanation:

What role does mutation play in the evolution of a species and the development of new characteristics?

Answers

Answer:

Mutation plays a vital part in evolution; it is the greatest source of genetic variation. In evolution, it creates a new DNA sequence for a particular gene, creating a new allele. Additionally, recombination can produce a DNA sequence (a new allele) for a specific gene through intragenic recombination.

Explanation:

Hope this helps!

How are oxygen and carbon dioxide exchanged between the alveoli and the
capillaries?
A. Endocytosis
B. Osmosis
C. Simple diffusion
5
D. Active transport

Answers

Answer:

B. Osmosis

Explanation:

Osmosis is the process in which oxygen and carbon dioxide exchanged occur between the alveoli and the  capillaries because the oxygen enters the body and the carbondioxide gas leaves the cell through a semi-permeable membrane and we know that Osmosis is a process in which smaller molecules moves from higher concentration to lower concentration through semi-permeable membrane of the cell.

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