Describe 2 limitations of a 3D cell model.

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Answers

Answer 1

Answer:

One of the major disadvantages of 3D cultures is that there is a requirement for the separation of single cells from spheroid structures by proteolytic degradation of single layers, which can take several hours or even a few days. There are many 3D methods where the efficiency, lifespan, repeatability, along with the comfort of work are poorer than in the case of 2D systems, which again is a disadvantage.

Explanation:

3D methods have become a lot more prevalent and are used in the area of cancer research, they can also be applied in tissue engineering. Cell culture is a commonly utilized in vitro tool for expanding our understanding of cell biology, tissue shape, disease mechanisms, medication action, protein production, and tissue engineering development. The majority of cancer biology research is based on in vitro experiments with two-dimensional (2D) cell cultures. However, because 2D cultures have numerous limits, 3D is utilised.

The following can be referred to for better understanding:

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Related Questions

The radiative zone is the outer shell of the sun from which light energy is radiated.

True
False

Answers

Answer:

False

Explanation:

The Radiative Zone is located just beyond the sun's Inner Core, at a distance of 0.25 to 0.7 solar radii. Photon emission and capture by hydrogen and helium ions cause energy to be radiated in this zone.

OAmalOHopeO

The answer is false. No explanation

Translate the words into math symbols. The difference of nine and a number is three. What steps would you take to translate the statement? Check all that apply. Replace "difference" with a division sign. Replace "difference" with a subtraction sign. Replace "a number" with the variable n, to represent an unknown value. Replace "is" with an equals sign. The translated equation is 9 ÷ n = 3. The translated equation is 9 – n = 3.

Answers

+•-my thing gotta be 20 things long tho

Answer:

Here are the correct answers that you should check.  

Replace "difference" with a subtraction sign.

Replace "a number" with the variable n, to represent an unknown value.

Replace "is" with an equals sign.

The translated equation is 9 – n = 3.

Explanation:

can there be rainbow fish

Answers

Answer:

Yes, Rainbow Trout

Explanation:

Answer:

there might be maybe scientist just haven't found them yet

hope this helps

Explanation:

Pls help................................

Answers

Answer:

from the lens

Explanation:

it easy gasbbsbsbbsbsbsbbsbsbssbsbssbsbsbbbssbsnns

Hehdnjdiens brisk w skxinenaiiz ebe

There are no nucleus and mitochondria in the red blood cells (RBCs). For this reason, RBCs are able to carry more hemoglobin molecules and deliver oxygen to the right place. Look at the following statements about RBCs: 1. RBCs cannot produce any ATP due to the lack of mitochondria 2. RBCs produce 2 molecules of ATP from 1 molecule of glucose 3. RBCs produce CO2 and water from glucose 4. RBCs produce lactic acid from glucose 5. RBCs produce ethyl alcohol and CO2 from glucose Which of the statement(s) is/are true? A. 2 and 4 B. 1 C. 3 and 5 D. 4 and 5 E. 2, 4 and 5

Answers

alright bet you can put a big boy
I think c is the answer

Elaborate on how an observation aids in the process of drawing a conclusion in the scientific method

Answers

Answer:

Restate: Restate the lab experiment. Describe the assignment.

Explain: Explain the purpose of the lab. ...

Results: Explain your results. ...

Uncertainties: Account for uncertainties and errors. ...

New: Discuss new questions or discoveries that emerged from the experiment.

Explanation:

Restate: Restate the lab experiment. Describe the assignment.

Explain: Explain the purpose of the lab....

Results: Explain your results....

Uncertainties: Account for uncertainties and errors. ...

New: Discuss new questions or discoveries that emerged from the experiment.

Explanation:

A camel is lost in the desert. It is not finding any food or water. It needs 9000 kilo calorie energy and 4 litre water daily. Its mass is 650 kg and 10% of its mass is fat. It has a water reservoir near its neck with a reserve of 312 litre water. It can live using its fat storage. 1g fat contains 9 kilo calorie energy. How long the camel can survive with it's reserve?

Answers

The correct answer to how long the camel can survive with its reserve would be 65 days.

First, let us calculate the total amount of energy derivable from the fat in the body of the animal:

Mass of the camel = 650 kgMass of fat = 10% of the total mass

              = 10% x 650 = 65 kg

If 1g of fat is equivalent to 9000 kilocalories of energy in the animal, then, 65 kg of fat would be equivalent to:

65 x 1000 x 9000   = 585,000 kilocalories of energy

The total amount of energy is 585,000 kilocalories and the total amount of reserved water is 312 litres:

Amount of energy required per day = 9000 kilocaloriesNumber of days 585,000 kilocalories of energy can serve = 585,000/9000

                                  = 65 days

Amount of water needed per day = 4 litresNumber of days 312 litres of water can serve = 312/4

                                                  = 78 days

The water reserve can last 78 days while the energy reserve can last 65 days. Since the camel needs both to survive, it means the animal can only survive for 65 days.

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Pls help this is an assignment!!
What is the length of a human cheek cell under the 100x objective lens of a microscope (aka 1000x magnification)?

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Answer:

When we look at cells under the microscope, our usual measurements fail to work. In  objectives we use (4 X, 10 X, 40 X and 100 X) arm.  

At 100 x magnification you will  see 2 mm. At 400 x magnification you will see 0.45 mm, or 450 microns. At 1000 x magnification you will  see 0.180 mm, or 180 microns.

Explanation:

In 3-5 sentances explain how the rate of photosynthesis or cellular respiration can be measured using products or reactants

Answers

The method most commonly used to measure the rate of photosynthesis and cellular respiration is by determining the amount of carbon dioxide (CO2) consumption and production, respectively. Both consumption and production of CO2 can be indirectly measured by using bromothymol, which is a pH indicator.

⦁ True or false?- Yogurt has good bacteria that is beneficial to humans

Answers

this is true :) i hope this helps
Answer: True

Explaination:

What codes for proteins?
amino acids O
RNA O
polypeptides O
DNA O ​

Answers

Answer:

DNA my friend

pls mark brainiest if it helped :)

Pretty sure the answer is DNA

Please help with this problem!

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I believe the answer is 3.

Hope this helped you! Please follow my account, thanks!

cool winds from the surrounding land

Which bright solar feature is shown in the picture above?

Solar flare
Prominence
Sunspot
Convection zone

Answers

Answer:

Prominence

Explanation:

Prominence is a huge, glistening, and gaseous feature that extends outward from the sun's surface. The photosphere is one of the layers of the sun where prominences are anchored before moving into the corona.

OAmalOHopeO

The answer is Prominence

Which is true of genes?

O They are sections of DNAthat produce the proteins that control an organism's characteristics

O They are proteins that produce sections of DNA which control the cell's activities

O They are features that can be observed rather than measured.

O They are the same as traits which show up in an organism if they are dominant​

Answers

the correct answer is: they are sections of DNA that produce the proteins that control an organisms characteristics

The statement that is true about Gene is that They are sections of DNA that produce the proteins that control an organism's characteristics.

What is Gene?

Gene refer to an hereditary unity which is transmitted from parent to their offsprings and it is transferred from one generation to another.

It is the major constituent of an organism DNA.

Therefore, The statement that is true about Gene is that They are sections of DNA that produce the proteins that control an organism's characteristics

Learn more about genes below.

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What is the name of each labeled part?

Answers

Answer:

ttyttgggyhj

gyyuf

gfthj

fghifvbb.

drjj

You can find the answer online by searching up the picture

what are the importance of agriculturist ? 4 points.

Please help!

Answers

Answer: HOPE IT WILL HELP U ...

Explanation:

1. It help to provide the direct employment to farmer.

2.It is the natural-friendly and most peaceful method of livelihood .

3.It is the primary source of the economic prior to the industrial revolution.

4.It contributes to the nations food  safety and health .

Agriculture plays a critical role in the entire life of a given economy. Agriculture is the backbone of the economic system of a given country. In addition to providing food and raw material, agriculture also provides employment opportunities to a very large percentage of the population.

HOPE SO THE GIVEN ANSWER HELPS YOU

Think of Earth as a ball with a rod through it, standing for the axis. This makes it easier to imagine Earths orientation in space relative to other objects like the Sun. Now consider the Sun as another ball. Imagine the Sun located in the center of a disk, kind of like a Frisbee. The planets in the solar system would be positioned on the Frisbee but at varying distances from the Sun. The area we are imagining as a Frisbee is actually called the plane of the ecliptic. It is the plane of Earths orbit around the Sun. Earths axis is not oriented perpendicular to the plane of its orbit. It is tilted at 23.5 degrees from the perpendicular. Why is this an important detail? This orientation in space, relative to the Sun, has great influence on Earths seasonal changes.

QUESTION 1
In paragraph 4, what word or phrase gives you a clue to what the word perpendicular means?

Somewhat like a Frisbee

Relative to the Sun

Rather it is tilted

Orientation in space

Answers

Explanation:

A ball is thrown straight up from a rooftop 320 feet high. The formula below describes the ball's height above the ground, h, in feet, t seconds after it was thrown. The ball misses the rooftop on its way down and eventually strikes the ground. How long will it take for the ball to hit the ground? Use this information to provide tick marks with appropriate numbers along the horizontal axis in the figure shown.

h=-16t^2+16t+320

The answer is “rather it is tilted”

What animal makes pepperoni?

Answers

sometimes it's made of cow and pig or it's just pig it just depends on what kind

Choose an organism. Use credible websites to research the classification of that organism and the characteristics its shares with other organisms you selected and full the table with information you gathered. PLSS HELP FAST!!

Answers

Kingdom: Anamalia
Phylum: Chordata
Class: Mammalia
Order: Artiodactyla
Family: Bovidae
Genus: Bos
Species: B. Taurus

The organism is Bovine (cattle, cow), the website is new world encyclopedia.

Hope this helps! Please make me the brainliest, it’s not necessary but appreciated, I put a lot of effort and research into my answers. Have a good day, stay safe and stay healthy.

PLEASE HELP WILL GIVE BRAINLEST

Analyze the given diagram of the carbon cycle below.


An image of carbon cycle is shown. The sun, a cloud, two trees, one on the left and the other on the right, an animal, lake, and a factory are shown in the image. An arrow is shown from the sun towards the left tree marked A. The sun is marked B. There is an arrow from the air above the clouds, marked C, towards the left tree. An arrow from a location close to the ground marked D points towards Dead Organisms, which is a label under the animal. An arrow marked E points from the right tree straight up to the clouds. An arrow marked F points from the animal straight up to the clouds. An arrow marked G points from the factory towards the air above the clouds, C. There is an arrow pointing from the air to the lake labeled Carbonates in Water, an arrow pointing down from dead organisms to Fossils and Fossil Fuels, and an arrow from Fossils to the factory.


Part 1: Which compound does C represent?

Part 2: Name a process that could release this compound into the air.

Part 3: Explain how the elements that form it are conserved during the carbon cycle. Use complete sentences to explain your answer.


Justify how this compound was created from a recycling of carbon in the carbon cycle. Use complete sentences to explain your answer.

Answers

I hope this can help some.

Choose one microscopic species that causes a human illness. Give the scientific name of the species, the disease it causes, and the vector, and then describe the symptoms in a 130 word paragraph or two.

Answers

Answer:

malaria

Explanation:

Mhm yup what they said

How are alleles for the same gene related?

O

A. They have the same genetic sequence but are found on different

chromosomes.

O

B. They have different genetic sequences and are found on different

chromosomes.

O

c. They have the same genetic sequence but are found in different

individuals

O

D. They have different genetic sequences but are found in the same

location on a chromosome.

SUBMIT

Answers

B!! i hope it works I got it right on edge
the correct answer is B

Think about how sedimentary rocks form. Can you determine which fossils in the image are the oldest and which fossils are the youngest? Explain ur answer. PLS HELP FAST!!!!

Answers

Answer:

Considering the formation of sedimentary rocks, the fossils in layer C are the oldest. Sedimentary rocks form in layers, with the oldest rock at the bottom. So, the fossils in layers A and B are newer than the ones in layer C. The fossils in layer A are the newest fossils

Explanation:

⦁ What technique can be used as the first step to identifying bacteria?

Answers

Answer:

Isolating a single bacterium species is the first step in identifying the bacteria possibly responsible for a disease process.

Explanation:

Following isolation, one of the first steps in identifying a bacterial isolate is the Gram stain, which allows for the determination of the Gram reaction, morphology, and arrangement of the organism.

THANK YOU!

I'm very bad at this o-o

Answers

It is c i got it right on a test
C & D are the chromosomes homologous

Which tissue of the chicken wing is commonly referred to as the meat? ​

Answers

Answer:

The muscle tissue on a chicken wing is meat. The same is true for most joints.

Explanation:

Answer:

The muscle surrounding bone is commonly referred to as the meat.

Which picture illustrates the telophase stage of mitosis?
Picture A, where spindle fibers start to pull away from O the center of the cell.

Picture B, where nuclear membranes form around each set of chromosomes resulting in two nuclei.

Picture C. where each pair of sister chromatids attaches to a spindle fiber by its centromere.

Picture D. where chromosomes coil up, nuclear membrane breaks down, and spindle fiber system forms.​

Answers

Answer:

Picture B,

Explanation:

where nuclear membranes form around each set of chromosomes resulting in two nuclei.

The correct option is B. Picture B, where nuclear membranes form around each set of chromosomes resulting in two nuclei.

What is telophase?

The last phase of cell division is known as telophase. The nuclear envelopes reform around the fresh nuclei in either half of the dividing cell during telophase.

The nucleus' ribosome-producing regions, or nucleolus, are back. The major components of the spindle apparatus begin to depolymerize as soon as the cell has finished transporting the chromosomes.

The chromosomes dissociate from their tightly bonded structure and return to free chromatin as telophase nears completion. Telophase is terminated by cytokinesis, a process that divides the cell into two new cells.

Therefore, The correct option is B. Picture B, where nuclear membranes form around each set of chromosomes resulting in two nuclei.

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Part 1: Which compound does C represent?
Part 2: Name a process that could release this compound into the air.
Part 3: Explain how the elements that form it are conserved during the carbon cycle. Use complete sentences to explain your answer.

Justify how this compound was created from a recycling of carbon in the carbon cycle. Use complete sentences to explain your answer.

Answers

I would go with Carbon dioxide
You should do carbon dioxide

Which of the following is likely to become a fossil
1. a skeleton in a large lake
2. a jellyfish in the ocean
3. a skeleton in a river bed that is drying up
4. an earthworm in a damp forest

Answers

Answer:

a skeleton in a river bed that is drying up

Number three a skeleton on a really bad strain

please help will mark brainliest for answer with explanation, but will report if rubbish answers are given
thank you​

Answers

Answer:

A is the cell surface membrane

B is the nucleus

C is the cytoplasm

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In which of the following triangles is cos B = 0.8?ABCD Pls Answer it for 50 points pls What is arsenic??? how does baby get its shape in the womb of an woman? One of the principles stated in the Declaration of Independence is that government shouldO have unlimited power to rule the peoplebe based upon the consent of the governedO guarantee economic equality among citizensbe led by educated citizens please helpppp. Which of these could be the graph of F(x) = In x + 3?A. Graph AB. Graph BC. Graph CD. Graph D Let S be a set of linearly dependent vectors in Rn. Select the best statement. A. The set S could, but does not have to, span Rn. B. The set S spans Rn, as long as no vector in S is a scalar multiple of another vector in the set. C. The set S cannot span Rn. D. The set S must span Rn. E. The set S does not span Rn if some vector in S is a scalar multiple of another vector in the set. F. The set S spans Rn, as long as it does not include the zero vector. G. none of the above B Page view A Read aloud Grammar Practice A' Did you go to the cinema last night (go) B No. 1 at home (stay) you ever to Mexico? (go) B Yes, I have last year. (go) A you any Latin American novels? (read) B Yes, 1 Spanish when i was at university (study) A What? you for dinner last night? (have) B Nothing 1 hungry (not be . Read the sentences carefully, Identify whether it is the simple past or the present perfect form. Change the words in the brackets into the correct forms of the simple past OR the present perfect tense. Write your answers in the following slide. Question no 1 is done for you. you ever to a famous actor? (speak) a famous person. (meet) 10 B No, 1 never AN you at the end of Titonic? (cry) very sad. (be) B Yes, it2 A 13 the new James Bond film? (see) you B No. Is it good? AH you your homework last night? (do) ING Rob cuts a circular hole out of a rectangular piece of paper. The paper measures 20 centimeters by 30 centimeters. The hole is 10 centimeters in diameter. How much of the piece of paper, in square centimeters, is left over after the hole is cut out? Choose five famous scientists and state their invention. (need to pass it asap) 2+4? I am omisha please give me answer watering flower trees .. my hobby.A. wereB.amC.areD.is URGENT !!!!!!!When the government puts restrictions on how credit card companies and financial institutions can behave with regard to who they can lend money or give credit, this reducing predatory lending, this is an example of which type of policy? (budget, monetary, regulatory, or fiscal?) pls write as much as possible ab it Match each term with the best description. a. Membrane-bound structure that supports energy production. b. Semi-permeable, living covering of the cell. c. Long, whip-like appendage used for locomotion. d. Membrane-bound structure containing the hereditary material of the cell. e. Gel-like substance encompassing the contents of the cell f. Basic structural and functional unit of living orgaisms1. Cell2. Cell membrane3. Cytoplasm4. Flagellum5. Mitochondrion6. Nucleus Escribe un anuncio, en espaol, sobre un prximo concierto de rock. Crea detalles ficticios sobre la banda, el lugar y la fecha del evento. Enumere los instrumentos y msicos, y describa la banda, su msica y el tipo de canciones del programa. Trate de usar el tiempo futuro tanto como sea posible please answer this question!! You are not able to go to the cinema with your friend because you are sick. Write approximately 100 ja* in Korean Help please!Find the inverse equation of this function f(x) = (x + 6)^2 + 1Thank you!! PLEASE HELP!!!! 30 POINTS & BRAINLIEST!!!! Answer the question below in one paragraph of at least four sentences. Your first sentence should state your opinion. Use specific evidence from this activity to support your answer. Evaluate the accuracy of the following claim:"The differences between India and Algeria show that economic imperialism is less damaging to a country than traditional, formal imperialism." Government works to maintain a competitive business environment by preventing __________.A.too many new companies going into business all at onceB.one business from unfairly taking over a marketC.competition from outside the USD.prices from becoming too high Jenna's company allows occasional personal use of work computers or phones as long as it isn't "excessive" or "inappropriate." Which action is most likely to be permitted? a) Using a company computer to search for a new job b) Missing a customer email because you have too many personal emails c) Being a few minutes late for a meeting because you're placing an online shopping order d) Calling your doctor to say you'll be late for an appointment after work